Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
Apprimus Verlag
2020
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Applied microbiology
Volume 21 |
| Schlagworte: | |
| Online-Zugang: | kostenfrei Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | XIII, 133 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 235 g |
| ISBN: | 9783863598624 3863598628 |
| DOI: | 10.18154/RWTH-2020-09097 |
Internformat
MARC
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| 100 | 1 | |a Otto, Maike |d 1989- |e Verfasser |0 (DE-588)1212611519 |4 aut | |
| 245 | 1 | 0 | |a Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine |c Maike Otto |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Aachen |b Apprimus Verlag |c 2020 | |
| 300 | |a XIII, 133 Seiten |b Illustrationen, Diagramme |c 21 cm x 14.8 cm, 235 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Applied microbiology |v Volume 21 | |
| 502 | |b Dissertation |c Rheinisch-Westfälische Technische Hochschule Aachen |d 2020 | ||
| 650 | 0 | 7 | |a Biotechnologie |0 (DE-588)4069491-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Phenylalanin |0 (DE-588)4174216-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pseudomonas |0 (DE-588)4210233-9 |2 gnd |9 rswk-swf |
| 653 | |a Microbial | ||
| 653 | |a catalysis | ||
| 653 | |a renewable | ||
| 653 | |a aromatics | ||
| 653 | |a metabolic | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Pseudomonas |0 (DE-588)4210233-9 |D s |
| 689 | 0 | 1 | |a Biotechnologie |0 (DE-588)4069491-4 |D s |
| 689 | 0 | 2 | |a Phenylalanin |0 (DE-588)4174216-3 |D s |
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| 830 | 0 | |a Applied microbiology |v Volume 21 |w (DE-604)BV042644516 |9 21 | |
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| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032189568 | ||
Datensatz im Suchindex
| _version_ | 1804181561329844224 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
SUMMARY
.....................................................................................................................................................................
ILL
ZUSAMMENFASSUNG
..................................................................................................................................................
V
LIST
OF
ABBREVIATIONS
...........................................................................................................................................
VII
LIST
OF
FIGURES
..........................................................................................................................................................
XI
LIST
OF
TABLES
..........................................................................................................................................................
XIII
1
GENERAL
INTRODUCTION
..................................................................................................................................
3
1.1
A
SHORT
HISTORY
OF
(MICRO)BIOLOGY
..........................................................................................................................
3
1.2
MICROBIAL
CATALYSIS
TODAY
........................................................................................................................................
6
1.3
AROMATICS
PRODUCTION
............................................................................................................................................
7
1.4
THE
POTENTIAL
OF
PSEUDOMONADS
AS
MICROBIAL
CELL
FACTORIES
...............................................................................
9
1.5
PSEUDOMONAS
SYNTHETIC
BIOLOGY
AND
TOOLS
..........................................................................................................
12
1.6
SCOPE
AND
AIM
OF
THIS
STUDY
.................................................................................................................................
14
2
MATERIAL
AND
METHODS
...............................................................................................................................
19
2.1
MEDIA
AND
CULTURE
CONDITIONS
..............................................................................................................................
19
2.2
BIOLECTOR
GROWTH
AND
FLUORESCENCE
ANALYSIS
....................................................................................................20
2.3
PLASMID
AND
STRAIN
CONSTRUCTION
AND
GENOMIC
MODIFICATION
..............................................................................20
2.4
FED-BATCH
IN
CONTROLLED
BIOREACTORS
....................................................................................................................
22
2.5
ANALYTICAL
METHODS
...............................................................................................................................................
22
2.6
RNA
QUANTIFICATION
VIA
Q-RT-PCR
........................................................................................................................
23
2.7
CONFIRMATION
OF
RIBOZYME
AUTOCATALYTIC
ACTIVITY
................................................................................................
23
2.8
EXPRESSION
STABILITY
TEST
.......................................................................................................................................
23
3
RESULTS
...............................................................................................................................................................
37
3.1
RATIONAL
ENGINEERING
OF
PHENYLALANINE
ACCUMULATION
IN
PSEUDOMONAS
TAIWANENSIS
TO
ENABLE
HIGH-YIELD
PRODUCTION
OF
TRANS-CINNAMATE
............................................................................................................................
37
3.1.1
ABSTRACT
.......................................................................................................................................................
37
3.1.2
INTRODUCTION
................................................................................................................................................
37
3.1.3
ENHANCED
TOLERANCE
OF
STREAMLINED
P.
TAIWANENSIS
CHASSIS
STRAINS
........................................................
39
3.1.4
RATIONAL
ENGINEERING
OF
L-PHENYLALANINE
PRODUCTION
..............................................................................
41
3.1.5
CINNAMATE
PRODUCTION
IN
SHAKE
FLASKS
.....................................................................................................
43
3.1.6
CINNAMATE
PRODUCTION
IN
CONTROLLED
BIOREACTORS
....................................................................................
45
3.1.7
CONCLUSION
...................................................................................................................................................48
3.2
EXPANDING
THE
PRODUCT
RANGE:
MICROBIAL
SYNTHESIS
OF
T-CINNAMATE-DERIVED
CHEMICALS
..................................
51
3.2.1
ABSTRACT
.......................................................................................................................................................
51
3.2.2
INTRODUCTION
................................................................................................................................................
51
3.2.3
RESULTS
AND
DISCUSSION
..............................................................................
53
3.2.3.1
STYRENE:
CULTIVATION
PROCEDURES
AND
TOXICITY
ASSESSMENT
............................................................
53
TABLE
OF
CONTENTS
3.23.2
STYRENE
PRODUCTION
WITH
PSEUDOMONAS
..........................................................................................
56
3.2.33
ASSESSMENT
OF
PINOSYLVIN
TOXICITY
.....................................................................................................
60
3.23.4
INVOLVEMENT
OF
THE
TTGGHI
SOLVENT
EFFLUX
PUMP
IN
PINOSYLVIN
TOLERANCE
......................................61
3.23.5
PINOSYLVIN
PRODUCTION
........................................................................................................................
62
3.23.6
BENZOATE,
CATECHOL,
AND
CIS,
C/S-MUCONATE
PRODUCTION
.................................................................66
3.2.4
CONCLUSION
......................................................................................................................................................
70
3.3
TARGETING
16S
RDNA
FOR
STABLE
RECOMBINANT
GENE
EXPRESSION
IN
PSEUDOMONAS
............................................
73
3.3.1
ABSTRACT
..........................................................................................................................................................
73
33.2
INTRODUCTION
..................................................................................................................................................
73
3.3.3
DEVELOPMENT
OF
A
TOOL
FOR
DIRECTED
INTEGRATION
AT
16S
RDNA
SITES
..........................................................
74
3.3.4
IMPROVING
MRNA
TRANSLATION
FOR
ENHANCED
PROTEIN
EXPRESSION
.............................................................77
3.3.5
EXPRESSION
ANALYSIS
OF
DIFFERENT
RRN
PROMOTERS
.........................................................................................
80
3.3.6
MULTIPLE
RRN
INTEGRATION
AND
STABILITY
ANALYSIS
..........................................................................................
82
3.3.7
CONCLUSION
....................................................................................................................................................
83
4
GENERAL
DISCUSSION
.......................................................................................................................................
87
4.1
FUTURE
APPLICATIONS
OF
L-PHENYLALANINE-OVERPRODUCING
CHASSIS
STRAINS
............................................................
87
4.2
ALTERNATIVE
FEEDSTOCKS
...........................................................................................................................................88
4.3
ENHANCING
MALONYL-COA
AVAILABILITY
FOR
IMPROVED
STILBENE
SYNTHESIS
...............................................................
90
4.4
CONCLUSION
..............................................................................................................................................................92
APPENDIX
.......................................................................................................................................................................
93
REFERENCES
................................................................................................................................................................
103
CURRICULUM
VITAE
..................................................................................................................................................
129
|
| adam_txt |
TABLE
OF
CONTENTS
SUMMARY
.
ILL
ZUSAMMENFASSUNG
.
V
LIST
OF
ABBREVIATIONS
.
VII
LIST
OF
FIGURES
.
XI
LIST
OF
TABLES
.
XIII
1
GENERAL
INTRODUCTION
.
3
1.1
A
SHORT
HISTORY
OF
(MICRO)BIOLOGY
.
3
1.2
MICROBIAL
CATALYSIS
TODAY
.
6
1.3
AROMATICS
PRODUCTION
.
7
1.4
THE
POTENTIAL
OF
PSEUDOMONADS
AS
MICROBIAL
CELL
FACTORIES
.
9
1.5
PSEUDOMONAS
SYNTHETIC
BIOLOGY
AND
TOOLS
.
12
1.6
SCOPE
AND
AIM
OF
THIS
STUDY
.
14
2
MATERIAL
AND
METHODS
.
19
2.1
MEDIA
AND
CULTURE
CONDITIONS
.
19
2.2
BIOLECTOR
GROWTH
AND
FLUORESCENCE
ANALYSIS
.20
2.3
PLASMID
AND
STRAIN
CONSTRUCTION
AND
GENOMIC
MODIFICATION
.20
2.4
FED-BATCH
IN
CONTROLLED
BIOREACTORS
.
22
2.5
ANALYTICAL
METHODS
.
22
2.6
RNA
QUANTIFICATION
VIA
Q-RT-PCR
.
23
2.7
CONFIRMATION
OF
RIBOZYME
AUTOCATALYTIC
ACTIVITY
.
23
2.8
EXPRESSION
STABILITY
TEST
.
23
3
RESULTS
.
37
3.1
RATIONAL
ENGINEERING
OF
PHENYLALANINE
ACCUMULATION
IN
PSEUDOMONAS
TAIWANENSIS
TO
ENABLE
HIGH-YIELD
PRODUCTION
OF
TRANS-CINNAMATE
.
37
3.1.1
ABSTRACT
.
37
3.1.2
INTRODUCTION
.
37
3.1.3
ENHANCED
TOLERANCE
OF
STREAMLINED
P.
TAIWANENSIS
CHASSIS
STRAINS
.
39
3.1.4
RATIONAL
ENGINEERING
OF
L-PHENYLALANINE
PRODUCTION
.
41
3.1.5
CINNAMATE
PRODUCTION
IN
SHAKE
FLASKS
.
43
3.1.6
CINNAMATE
PRODUCTION
IN
CONTROLLED
BIOREACTORS
.
45
3.1.7
CONCLUSION
.48
3.2
EXPANDING
THE
PRODUCT
RANGE:
MICROBIAL
SYNTHESIS
OF
T-CINNAMATE-DERIVED
CHEMICALS
.
51
3.2.1
ABSTRACT
.
51
3.2.2
INTRODUCTION
.
51
3.2.3
RESULTS
AND
DISCUSSION
.
53
3.2.3.1
STYRENE:
CULTIVATION
PROCEDURES
AND
TOXICITY
ASSESSMENT
.
53
TABLE
OF
CONTENTS
3.23.2
STYRENE
PRODUCTION
WITH
PSEUDOMONAS
.
56
3.2.33
ASSESSMENT
OF
PINOSYLVIN
TOXICITY
.
60
3.23.4
INVOLVEMENT
OF
THE
TTGGHI
SOLVENT
EFFLUX
PUMP
IN
PINOSYLVIN
TOLERANCE
.61
3.23.5
PINOSYLVIN
PRODUCTION
.
62
3.23.6
BENZOATE,
CATECHOL,
AND
CIS,
C/S-MUCONATE
PRODUCTION
.66
3.2.4
CONCLUSION
.
70
3.3
TARGETING
16S
RDNA
FOR
STABLE
RECOMBINANT
GENE
EXPRESSION
IN
PSEUDOMONAS
.
73
3.3.1
ABSTRACT
.
73
33.2
INTRODUCTION
.
73
3.3.3
DEVELOPMENT
OF
A
TOOL
FOR
DIRECTED
INTEGRATION
AT
16S
RDNA
SITES
.
74
3.3.4
IMPROVING
MRNA
TRANSLATION
FOR
ENHANCED
PROTEIN
EXPRESSION
.77
3.3.5
EXPRESSION
ANALYSIS
OF
DIFFERENT
RRN
PROMOTERS
.
80
3.3.6
MULTIPLE
RRN
INTEGRATION
AND
STABILITY
ANALYSIS
.
82
3.3.7
CONCLUSION
.
83
4
GENERAL
DISCUSSION
.
87
4.1
FUTURE
APPLICATIONS
OF
L-PHENYLALANINE-OVERPRODUCING
CHASSIS
STRAINS
.
87
4.2
ALTERNATIVE
FEEDSTOCKS
.88
4.3
ENHANCING
MALONYL-COA
AVAILABILITY
FOR
IMPROVED
STILBENE
SYNTHESIS
.
90
4.4
CONCLUSION
.92
APPENDIX
.
93
REFERENCES
.
103
CURRICULUM
VITAE
.
129 |
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| author | Otto, Maike 1989- |
| author_GND | (DE-588)1212611519 |
| author_facet | Otto, Maike 1989- |
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| author_sort | Otto, Maike 1989- |
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| building | Verbundindex |
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| discipline | Biologie |
| discipline_str_mv | Biologie |
| doi_str_mv | 10.18154/RWTH-2020-09097 |
| edition | 1. Auflage |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV046780374 |
| illustrated | Illustrated |
| index_date | 2024-07-03T14:49:39Z |
| indexdate | 2024-07-10T08:53:37Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783863598624 3863598628 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032189568 |
| oclc_num | 1164641916 |
| open_access_boolean | 1 |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | XIII, 133 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 235 g |
| psigel | ebook |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Apprimus Verlag |
| record_format | marc |
| series | Applied microbiology |
| series2 | Applied microbiology |
| spelling | Otto, Maike 1989- Verfasser (DE-588)1212611519 aut Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine Maike Otto 1. Auflage Aachen Apprimus Verlag 2020 XIII, 133 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 235 g txt rdacontent n rdamedia nc rdacarrier Applied microbiology Volume 21 Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2020 Biotechnologie (DE-588)4069491-4 gnd rswk-swf Phenylalanin (DE-588)4174216-3 gnd rswk-swf Pseudomonas (DE-588)4210233-9 gnd rswk-swf Microbial catalysis renewable aromatics metabolic (DE-588)4113937-9 Hochschulschrift gnd-content Pseudomonas (DE-588)4210233-9 s Biotechnologie (DE-588)4069491-4 s Phenylalanin (DE-588)4174216-3 s DE-604 Apprimus Verlag (DE-588)1068101474 pbl Erscheint auch als Online-Ausgabe, PDF https://doi.org/10.18154/RWTH-2020-09097 Applied microbiology Volume 21 (DE-604)BV042644516 21 https://doi.org/10.18154/RWTH-2020-09097 Resolving-System kostenfrei Volltext X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=c16c6031c25e4e318f4417ea13278796&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032189568&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Otto, Maike 1989- Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine Applied microbiology Biotechnologie (DE-588)4069491-4 gnd Phenylalanin (DE-588)4174216-3 gnd Pseudomonas (DE-588)4210233-9 gnd |
| subject_GND | (DE-588)4069491-4 (DE-588)4174216-3 (DE-588)4210233-9 (DE-588)4113937-9 |
| title | Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine |
| title_auth | Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine |
| title_exact_search | Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine |
| title_exact_search_txtP | Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine |
| title_full | Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine Maike Otto |
| title_fullStr | Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine Maike Otto |
| title_full_unstemmed | Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine Maike Otto |
| title_short | Microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine |
| title_sort | microbial catalysis of renewable resources into aromatics via the central metabolic precursor phenylalanine |
| topic | Biotechnologie (DE-588)4069491-4 gnd Phenylalanin (DE-588)4174216-3 gnd Pseudomonas (DE-588)4210233-9 gnd |
| topic_facet | Biotechnologie Phenylalanin Pseudomonas Hochschulschrift |
| url | https://doi.org/10.18154/RWTH-2020-09097 http://deposit.dnb.de/cgi-bin/dokserv?id=c16c6031c25e4e318f4417ea13278796&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032189568&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV042644516 |
| work_keys_str_mv | AT ottomaike microbialcatalysisofrenewableresourcesintoaromaticsviathecentralmetabolicprecursorphenylalanine AT apprimusverlag microbialcatalysisofrenewableresourcesintoaromaticsviathecentralmetabolicprecursorphenylalanine |