Tailored poly-γ-glutamic acid production with Bacillus subtilis 168:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
Apprimus Verlag
2020
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Applied microbiology
Volume 18 |
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | XXI, 209 Seiten Illustrationen 21 cm, 339 g |
| ISBN: | 9783863598327 3863598326 |
Internformat
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| 100 | 1 | |a Halmschlag, Birthe |d 1990- |e Verfasser |0 (DE-588)1208802070 |4 aut | |
| 245 | 1 | 0 | |a Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 |c Birthe Halmschlag |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Aachen |b Apprimus Verlag |c 2020 | |
| 300 | |a XXI, 209 Seiten |b Illustrationen |c 21 cm, 339 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Applied microbiology |v Volume 18 | |
| 502 | |b Dissertation |c RWTH Aachen University |d 2019 | ||
| 650 | 0 | 7 | |a Polyglutaminsäure |0 (DE-588)4467504-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gentechnisch veränderter Organismus |0 (DE-588)4353579-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ausgangsmaterial |0 (DE-588)4143518-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Herstellung |0 (DE-588)4159653-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biomasse |0 (DE-588)4006877-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Glucose |0 (DE-588)4157675-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biokonversion |0 (DE-588)4145610-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Xylose |0 (DE-588)4190397-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Heubacillus |0 (DE-588)4242465-3 |2 gnd |9 rswk-swf |
| 653 | |a Naturwissenschaften | ||
| 653 | |a Poly | ||
| 653 | |a glutamatic | ||
| 653 | |a acid | ||
| 653 | |a Bacillus | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Polyglutaminsäure |0 (DE-588)4467504-5 |D s |
| 689 | 0 | 1 | |a Herstellung |0 (DE-588)4159653-5 |D s |
| 689 | 0 | 2 | |a Biomasse |0 (DE-588)4006877-8 |D s |
| 689 | 0 | 3 | |a Glucose |0 (DE-588)4157675-5 |D s |
| 689 | 0 | 4 | |a Xylose |0 (DE-588)4190397-3 |D s |
| 689 | 0 | 5 | |a Ausgangsmaterial |0 (DE-588)4143518-7 |D s |
| 689 | 0 | 6 | |a Biokonversion |0 (DE-588)4145610-5 |D s |
| 689 | 0 | 7 | |a Heubacillus |0 (DE-588)4242465-3 |D s |
| 689 | 0 | 8 | |a Gentechnisch veränderter Organismus |0 (DE-588)4353579-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 710 | 2 | |a Apprimus Verlag |0 (DE-588)1068101474 |4 pbl | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |a Halmschlag, Birthe, 1990- |t Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 |b 1. Auflage |d Aachen : Apprimus Wissenschaftsverlag, 2020 |h Online-Ressource, 242 Seiten |
| 830 | 0 | |a Applied microbiology |v Volume 18 |w (DE-604)BV042644516 |9 18 | |
| 856 | 4 | 2 | |m X:MVB |q text/html |u http://deposit.dnb.de/cgi-bin/dokserv?id=07527f0612d346b78676d7d33159fd23&prov=M&dok_var=1&dok_ext=htm |3 Inhaltstext |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1207328464/04 |3 Inhaltsverzeichnis |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-032187584 | ||
Datensatz im Suchindex
| _version_ | 1804181557897854976 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
SUMMARY
.....................................................................................................................................
VII
ZUSAMMENFASSUNG
.........................................................................................................................IX
LIST
OF
ABBREVIATIONS
...................................................................................................................
XIII
LIST
OF
FIGURES
............................................................................................................................
XVII
LIST
OF
TABLES
...............................................................................................................................
XXI
1
GENERAL
INTRODUCTION
...............................................................................................................
1
1.1
METABOLIC
ENGINEERING
.....................................................................................................
3
1.2
COMPUTATIONAL
BASED
DESIGN
OF
METABOLIC
NETWORKS
.....................................................
4
1.2.1
METABOLIC
MODEL
DEVELOPMENT
................................................................................
5
1.2.2
*
OMICS
*
TECHNOLOGIES
FOR
METABOLIC
MODEL
CONSTRUCTION
.......................................
6
1.2.3
MODEL-DRIVEN
METABOLIC
ENGINEERING
......................................................................
7
1.3
MOLECULAR
CONSTRUCTION
OF
PRODUCTION
STRAINS
.................................................................
9
1.3.1
MOLECULAR
BIOLOGY
TOOLS
...........................................................................................
9
1.3.2
GENETIC
ENGINEERING
OF
MODEL
ORGANISM
BACILLUS
SUBTILIS
.....................................
13
1.3.3
MOLECULAR
MANIPULATION
OF
AUTOTROPHIC
ORGANISMS
..............................................
14
1.4
PERFORMANCE
TESTING
........................................................................................................
15
1.4.1
MINIATURIZATION
AND
PARALLELIZATION
.......................................................................
15
1.4.2
HIGH
CONTENT
CHARACTERIZATION
OF
IMPROVED
STRAINS
BY
METABOLIC
FLUX
ANALYSIS
.
18
1.5
SCOPE
AND
OUTLINE
OF
THIS
THESIS
.....................................................................................
22
2
RESULTS
AND
DISCUSSION
...........................................................................................................
27
2.1
POLY-Y-GLUTAMIC
ACID
PRODUCTION
WITH
BACILLUS
SUBTILIS
168
........................................
27
2.1.1
SUMMARY
.................................................................................................................
29
2.1.2
INTRODUCTION
..............................................................................................................
29
2.1.3
RESULTS
.....................................................................................................................
31
2.1.4
DISCUSSION
...............................................................................................................
40
TABLE
OF
CONTENTS
2.2
METABOLIC
ENGINEERING
FOR
ENHANCED
Y-PGA
SYNTHESIS
................................................
45
2.2.1
SUMMARY
..................................................................................................................
47
2.2.2
INTRODUCTION
..............................................................................................................
47
2.2.3
RESULTS
......................................................................................................................49
2.2.4
DISCUSSION
................................................................................................................
63
2.3
IDENTIFICATION
OF
KEY
METABOLITES
IN
POLY-Y-GLUTAMIC
ACID
PRODUCTION
BY
TIMING
Y-PGA
SYNTHETASE
EXPRESSION
..................................................................................................................
67
2.3.1
SUMMARY
..................................................................................................................
69
2.3.2
INTRODUCTION
..............................................................................................................
69
2.3.3
RESULTS
......................................................................................................................
71
2.3.4
DISCUSSION
................................................................................................................79
2.4
TAILOR-MADE
POLY-Y-GLUTAMIC
ACID
PRODUCTION
..............................................................
85
2.4.1
SUMMARY
..................................................................................................................
87
2.4.2
INTRODUCTION
..............................................................................................................
87
2.4.3
RESULTS
AND
DISCUSSION
............................................................................................
89
2.4.4
CONCLUSION
................................................................................................................
99
2.5
RESOURCE
EFFICIENT
POLY-Y-GLUTAMIC
ACID
PRODUCTION
BY
METABOLIC
ENGINEERING
OF
BACILLUS
SUBTILIS
FOR
XYLOSE
UTILIZATION
.....................................................................................
101
2.5.1
SUMMARY
................................................................................................................
103
2.5.2
INTRODUCTION
............................................................................................................
103
2.5.3
RESULTS
.....................................................................................................................
105
2.5.4
DISCUSSION
..............................................................................................................
121
3
GENERAL
DISCUSSION
AND
OUTLOOK
...........................................................................................
127
3.1
MICROBIAL
BIOPOLYMER
PRODUCTION
................................................................................
127
3.2
BACILLUS
SUBTILIS
AS
Y-PGA
PRODUCTION
HOST
.................................................................
128
3.3
METABOLIC
ENGINEERING
ENHANCING
Y-PGA
PRODUCTION
...............................................
129
3.4
UTILIZATION
OF
RENEWABLE
SUBSTRATE
..............................................................................
131
II
TABLE
OF
CONTENTS
3.5
METABOLOME
ANALYSIS
....................................................................................................
133
3.6
TAILOR-MADE
Y-PGA
PRODUCTION
...................................................................................
133
3.7
CONCLUSIONS
&
OUTLOOK
................................................................................................
134
4
MATERIAL
AND
METHODS
..........................................................................................................
139
4.1
CHEMICALS
......................................................................................................................
139
4.2
STRAINS,
MEDIA
AND
CULTURE
CONDITIONS
...........................................................................
139
4.3
BATCH
FERMENTATIONS
......................................................................................................
146
4.4
CONTINUOUS
FERMENTATION
.............................................................................................
147
4.5
MOLECULAR
BIOLOGY
METHODS
.........................................................................................
148
4.5.1
DNA
TECHNIQUES
....................................................................................................
148
4.5.2
GENOME
ENGINEERING
IN
B.
SUBTILIS
......................................................................
164
4.6
ANALYTICAL
METHODS
.......................................................................................................
169
4.6.1
AGAROSE
GEL
ELECTROPHORESIS
.................................................................................
169
4.6.2
HPLC
ANALYSIS
.......................................................................................................
169
4.6.3
DETERMINATION
OF
PROMOTER
ACTIVITY
USING
GFP
...................................................
169
4.6.4
METABOLIC
MODELING
...............................................................................................
170
4.6.5
DETERMINATION
OF
Y-PGA
TITER
BY
CETYLTRIMETHYLAMMONIUM
BROMIDE
ASSAY
....
170
4.6.6
DETERMINATION
OF
Y-PGA
MOLECULAR
WEIGHT
AND
CONCENTRATION
BY
GEL
PERMEATION
CHROMATOGRAPHY
..................................................................................................................
171
4.6.7
DETERMINATION
OF
Y-PGA
CONCENTRATION
BY
GAS
CHROMATOGRAPHY
......................
171
4.6.8
DETERMINATION
OF
THE
STEREOCHEMICAL
COMPOSITION
OF
Y-PGA
BY
LC-MS
.........
172
4.6.9
SAMPLE
PREPARATION
FOR
METABOLOME
ANALYSIS
.....................................................
172
4.6.10
METABOLOME
ANALYSIS
BY
ION
PAIR-LC/MS/MS
....................................................
173
4.6.11
METABOLITE
DATA
PROCESSING
AND
ANALYSIS
..............................................................
173
5
REFERENCES
............................................................................................................................
177
III
|
| adam_txt |
TABLE
OF
CONTENTS
SUMMARY
.
VII
ZUSAMMENFASSUNG
.IX
LIST
OF
ABBREVIATIONS
.
XIII
LIST
OF
FIGURES
.
XVII
LIST
OF
TABLES
.
XXI
1
GENERAL
INTRODUCTION
.
1
1.1
METABOLIC
ENGINEERING
.
3
1.2
COMPUTATIONAL
BASED
DESIGN
OF
METABOLIC
NETWORKS
.
4
1.2.1
METABOLIC
MODEL
DEVELOPMENT
.
5
1.2.2
*
OMICS
*
TECHNOLOGIES
FOR
METABOLIC
MODEL
CONSTRUCTION
.
6
1.2.3
MODEL-DRIVEN
METABOLIC
ENGINEERING
.
7
1.3
MOLECULAR
CONSTRUCTION
OF
PRODUCTION
STRAINS
.
9
1.3.1
MOLECULAR
BIOLOGY
TOOLS
.
9
1.3.2
GENETIC
ENGINEERING
OF
MODEL
ORGANISM
BACILLUS
SUBTILIS
.
13
1.3.3
MOLECULAR
MANIPULATION
OF
AUTOTROPHIC
ORGANISMS
.
14
1.4
PERFORMANCE
TESTING
.
15
1.4.1
MINIATURIZATION
AND
PARALLELIZATION
.
15
1.4.2
HIGH
CONTENT
CHARACTERIZATION
OF
IMPROVED
STRAINS
BY
METABOLIC
FLUX
ANALYSIS
.
18
1.5
SCOPE
AND
OUTLINE
OF
THIS
THESIS
.
22
2
RESULTS
AND
DISCUSSION
.
27
2.1
POLY-Y-GLUTAMIC
ACID
PRODUCTION
WITH
BACILLUS
SUBTILIS
168
.
27
2.1.1
SUMMARY
.
29
2.1.2
INTRODUCTION
.
29
2.1.3
RESULTS
.
31
2.1.4
DISCUSSION
.
40
TABLE
OF
CONTENTS
2.2
METABOLIC
ENGINEERING
FOR
ENHANCED
Y-PGA
SYNTHESIS
.
45
2.2.1
SUMMARY
.
47
2.2.2
INTRODUCTION
.
47
2.2.3
RESULTS
.49
2.2.4
DISCUSSION
.
63
2.3
IDENTIFICATION
OF
KEY
METABOLITES
IN
POLY-Y-GLUTAMIC
ACID
PRODUCTION
BY
TIMING
Y-PGA
SYNTHETASE
EXPRESSION
.
67
2.3.1
SUMMARY
.
69
2.3.2
INTRODUCTION
.
69
2.3.3
RESULTS
.
71
2.3.4
DISCUSSION
.79
2.4
TAILOR-MADE
POLY-Y-GLUTAMIC
ACID
PRODUCTION
.
85
2.4.1
SUMMARY
.
87
2.4.2
INTRODUCTION
.
87
2.4.3
RESULTS
AND
DISCUSSION
.
89
2.4.4
CONCLUSION
.
99
2.5
RESOURCE
EFFICIENT
POLY-Y-GLUTAMIC
ACID
PRODUCTION
BY
METABOLIC
ENGINEERING
OF
BACILLUS
SUBTILIS
FOR
XYLOSE
UTILIZATION
.
101
2.5.1
SUMMARY
.
103
2.5.2
INTRODUCTION
.
103
2.5.3
RESULTS
.
105
2.5.4
DISCUSSION
.
121
3
GENERAL
DISCUSSION
AND
OUTLOOK
.
127
3.1
MICROBIAL
BIOPOLYMER
PRODUCTION
.
127
3.2
BACILLUS
SUBTILIS
AS
Y-PGA
PRODUCTION
HOST
.
128
3.3
METABOLIC
ENGINEERING
ENHANCING
Y-PGA
PRODUCTION
.
129
3.4
UTILIZATION
OF
RENEWABLE
SUBSTRATE
.
131
II
TABLE
OF
CONTENTS
3.5
METABOLOME
ANALYSIS
.
133
3.6
TAILOR-MADE
Y-PGA
PRODUCTION
.
133
3.7
CONCLUSIONS
&
OUTLOOK
.
134
4
MATERIAL
AND
METHODS
.
139
4.1
CHEMICALS
.
139
4.2
STRAINS,
MEDIA
AND
CULTURE
CONDITIONS
.
139
4.3
BATCH
FERMENTATIONS
.
146
4.4
CONTINUOUS
FERMENTATION
.
147
4.5
MOLECULAR
BIOLOGY
METHODS
.
148
4.5.1
DNA
TECHNIQUES
.
148
4.5.2
GENOME
ENGINEERING
IN
B.
SUBTILIS
.
164
4.6
ANALYTICAL
METHODS
.
169
4.6.1
AGAROSE
GEL
ELECTROPHORESIS
.
169
4.6.2
HPLC
ANALYSIS
.
169
4.6.3
DETERMINATION
OF
PROMOTER
ACTIVITY
USING
GFP
.
169
4.6.4
METABOLIC
MODELING
.
170
4.6.5
DETERMINATION
OF
Y-PGA
TITER
BY
CETYLTRIMETHYLAMMONIUM
BROMIDE
ASSAY
.
170
4.6.6
DETERMINATION
OF
Y-PGA
MOLECULAR
WEIGHT
AND
CONCENTRATION
BY
GEL
PERMEATION
CHROMATOGRAPHY
.
171
4.6.7
DETERMINATION
OF
Y-PGA
CONCENTRATION
BY
GAS
CHROMATOGRAPHY
.
171
4.6.8
DETERMINATION
OF
THE
STEREOCHEMICAL
COMPOSITION
OF
Y-PGA
BY
LC-MS
.
172
4.6.9
SAMPLE
PREPARATION
FOR
METABOLOME
ANALYSIS
.
172
4.6.10
METABOLOME
ANALYSIS
BY
ION
PAIR-LC/MS/MS
.
173
4.6.11
METABOLITE
DATA
PROCESSING
AND
ANALYSIS
.
173
5
REFERENCES
.
177
III |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Halmschlag, Birthe 1990- |
| author_GND | (DE-588)1208802070 |
| author_facet | Halmschlag, Birthe 1990- |
| author_role | aut |
| author_sort | Halmschlag, Birthe 1990- |
| author_variant | b h bh |
| building | Verbundindex |
| bvnumber | BV046778330 |
| ctrlnum | (OCoLC)1164659009 (DE-599)DNB1207328464 |
| dewey-full | 660.62 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.62 |
| dewey-search | 660.62 |
| dewey-sort | 3660.62 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| discipline_str_mv | Chemie / Pharmazie |
| edition | 1. Auflage |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV046778330 |
| illustrated | Illustrated |
| index_date | 2024-07-03T14:49:07Z |
| indexdate | 2024-07-10T08:53:33Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783863598327 3863598326 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032187584 |
| oclc_num | 1164659009 |
| open_access_boolean | |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | XXI, 209 Seiten Illustrationen 21 cm, 339 g |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Apprimus Verlag |
| record_format | marc |
| series | Applied microbiology |
| series2 | Applied microbiology |
| spelling | Halmschlag, Birthe 1990- Verfasser (DE-588)1208802070 aut Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 Birthe Halmschlag 1. Auflage Aachen Apprimus Verlag 2020 XXI, 209 Seiten Illustrationen 21 cm, 339 g txt rdacontent n rdamedia nc rdacarrier Applied microbiology Volume 18 Dissertation RWTH Aachen University 2019 Polyglutaminsäure (DE-588)4467504-5 gnd rswk-swf Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd rswk-swf Ausgangsmaterial (DE-588)4143518-7 gnd rswk-swf Herstellung (DE-588)4159653-5 gnd rswk-swf Biomasse (DE-588)4006877-8 gnd rswk-swf Glucose (DE-588)4157675-5 gnd rswk-swf Biokonversion (DE-588)4145610-5 gnd rswk-swf Xylose (DE-588)4190397-3 gnd rswk-swf Heubacillus (DE-588)4242465-3 gnd rswk-swf Naturwissenschaften Poly glutamatic acid Bacillus (DE-588)4113937-9 Hochschulschrift gnd-content Polyglutaminsäure (DE-588)4467504-5 s Herstellung (DE-588)4159653-5 s Biomasse (DE-588)4006877-8 s Glucose (DE-588)4157675-5 s Xylose (DE-588)4190397-3 s Ausgangsmaterial (DE-588)4143518-7 s Biokonversion (DE-588)4145610-5 s Heubacillus (DE-588)4242465-3 s Gentechnisch veränderter Organismus (DE-588)4353579-3 s DE-604 Apprimus Verlag (DE-588)1068101474 pbl Erscheint auch als Online-Ausgabe Halmschlag, Birthe, 1990- Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 1. Auflage Aachen : Apprimus Wissenschaftsverlag, 2020 Online-Ressource, 242 Seiten Applied microbiology Volume 18 (DE-604)BV042644516 18 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=07527f0612d346b78676d7d33159fd23&prov=M&dok_var=1&dok_ext=htm Inhaltstext B:DE-101 application/pdf https://d-nb.info/1207328464/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032187584&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Halmschlag, Birthe 1990- Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 Applied microbiology Polyglutaminsäure (DE-588)4467504-5 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Ausgangsmaterial (DE-588)4143518-7 gnd Herstellung (DE-588)4159653-5 gnd Biomasse (DE-588)4006877-8 gnd Glucose (DE-588)4157675-5 gnd Biokonversion (DE-588)4145610-5 gnd Xylose (DE-588)4190397-3 gnd Heubacillus (DE-588)4242465-3 gnd |
| subject_GND | (DE-588)4467504-5 (DE-588)4353579-3 (DE-588)4143518-7 (DE-588)4159653-5 (DE-588)4006877-8 (DE-588)4157675-5 (DE-588)4145610-5 (DE-588)4190397-3 (DE-588)4242465-3 (DE-588)4113937-9 |
| title | Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 |
| title_auth | Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 |
| title_exact_search | Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 |
| title_exact_search_txtP | Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 |
| title_full | Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 Birthe Halmschlag |
| title_fullStr | Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 Birthe Halmschlag |
| title_full_unstemmed | Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 Birthe Halmschlag |
| title_short | Tailored poly-γ-glutamic acid production with Bacillus subtilis 168 |
| title_sort | tailored poly γ glutamic acid production with bacillus subtilis 168 |
| topic | Polyglutaminsäure (DE-588)4467504-5 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Ausgangsmaterial (DE-588)4143518-7 gnd Herstellung (DE-588)4159653-5 gnd Biomasse (DE-588)4006877-8 gnd Glucose (DE-588)4157675-5 gnd Biokonversion (DE-588)4145610-5 gnd Xylose (DE-588)4190397-3 gnd Heubacillus (DE-588)4242465-3 gnd |
| topic_facet | Polyglutaminsäure Gentechnisch veränderter Organismus Ausgangsmaterial Herstellung Biomasse Glucose Biokonversion Xylose Heubacillus Hochschulschrift |
| url | http://deposit.dnb.de/cgi-bin/dokserv?id=07527f0612d346b78676d7d33159fd23&prov=M&dok_var=1&dok_ext=htm https://d-nb.info/1207328464/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032187584&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV042644516 |
| work_keys_str_mv | AT halmschlagbirthe tailoredpolygglutamicacidproductionwithbacillussubtilis168 AT apprimusverlag tailoredpolygglutamicacidproductionwithbacillussubtilis168 |