The forbidden subject: how oppositional aesthetics banished natural beauty from the arts
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridgeshire, UK
The White Horse Press
2019
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 275 Seiten Illustrationen 24 cm |
| ISBN: | 9781912186099 |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV046708280 | ||
| 003 | DE-604 | ||
| 005 | 20200721 | ||
| 007 | t | ||
| 008 | 200506s2019 a||| b||| 00||| eng d | ||
| 020 | |a 9781912186099 |c Hardback |9 978-1-912186-09-9 | ||
| 035 | |a (OCoLC)1190676443 | ||
| 035 | |a (DE-599)BVBBV046708280 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-19 | ||
| 084 | |a AR 14500 |0 (DE-625)8329: |2 rvk | ||
| 100 | 1 | |a Quigley, Peter |d 1951- |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a The forbidden subject |b how oppositional aesthetics banished natural beauty from the arts |c Peter Quigley |
| 264 | 1 | |a Cambridgeshire, UK |b The White Horse Press |c 2019 | |
| 300 | |a 275 Seiten |b Illustrationen |c 24 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 336 | |b sti |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 653 | 0 | |a Nature (Aesthetics) | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032118719&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 940 | 1 | |q UBM-RCC | |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032118719 | ||
Datensatz im Suchindex
| _version_ | 1804181440825393152 |
|---|---|
| adam_text | TABLE
OF
CONTENT
ABSTRACT
................................................................................................................................
1
1.
INTRODUCTION
.....................................................................................................................
2
1.1.
THE
RESPIRATORY
SYSTEM
...........................................................................................
2
1.1.1.
LUNG
DEVELOPMENT
...........................................................................................
4
1.1.2.
CYSTIC
FIBROSIS
..................................................................................................
5
1.1.3.
THERAPEUTIC
OPTIONS
FOR
CYSTIC
FIBROSIS
............................................................
6
1.2.
HUMAN
INDUCED
PLURIPOTENT
STEM
CELLS
IN
REGENERATIVE
MEDICINE
........................
7
1.2.1.
DIRECTED
DIFFERENTIATION
OF
HIPSCS
INTO
LUNG
EPITHELIAL
CELLS
...........................
9
1.3.
METHODS
FOR
GENERATION
OF
REPORTER
CELL
LINES
........................................................
11
1.3.1.
KNOCKIN
HUMAN
PLURIPOTENT
STEM
CELL
REPORTER
LINES
IN
REGENERATIVE
MEDICINE
..........................................
13
2.
AIMS
OF
THE
STUDY
.........................................................................................................
15
3.
MATERIAL
..........................................................................................................................
16
3.1.
CHEMICALS
...............................................................................................................
16
3.2.
PRIMARY
ANTIBODIES
.................................................................................................
17
3.3.
ISOTYPE
CONTROLS
.......................................................................................................
19
3.4.
SECONDARY
ANTIBODIES
............................................................................................
19
3.5.
CULTURE
MEDIA
........................................................................................................
20
3.6.
BUFFERS
....................................................................................................................
21
3.7.
VECTORS
&
TALENS
.................................................................................................
22
3.7.1.
NKX2.1
TARGETING
VECTOR
................................................................................
22
3.7.2.
P63
TARGETING
VECTOR
.......................................................................................
23
3.7.3.
CCSP
TARGETING
VECTOR
...................................................................................
24
3.7.4.
FLP
RECOMBINASE
EXPRESSING
VECTOR
...............................
25
3.7.5.
TALENS
..........................................................................................................
26
3.8.
BACTERIA
..................................................................................................................
27
3.9.
PRIMER
.....................................................................................................................
27
3.10.
CELL
LINES
...............................................................................................................
29
3.10.1.
MOUSE
EMBRYONIC
FIBROBLASTS
......................................................................
29
3.10.2.
HHSC_F1285_T-IPS2
.................................................................................
29
4.
METHODS
........................................................................................................................
30
IV
4.1.
CELL
CULTURE
30
4.1.1.
MOUSE
EMBRYONIC
FIBROBLASTS
(MEFS)
30
4.1.2.
CULTIVATION
OF
UNDIFFERENTIATED
HIPSCS
30
4.2.
DIRECTED
DIFFERENTIATION
OF
HIPSCS
31
4.3.
FLOW
CYTOMETRY
STAINING
33
4.4.
IMMUNOFLUORESCENCE
STAINING
34
4.5.
REPORTER
CELL
LINE
GENERATION
35
4.6.
UNDIRECTED
DIFFERENTIATION
OF
HIPSCS
INTO
DERIVATIVES
OF
ALL
THREE
GERM
LAYERS
35
4.7.
KARYOTYPE
ANALYSIS
36
4.8.
MRNA
EXPRESSION
ANALYSIS
36
4.9.
TRANSMISSION
ELECTRON
MICROSCOPY
37
4.10.
STATISTICAL
ANALYSIS
37
5.
RESULTS
38
5.1.
GENERATION
OF
A
HIPSC-NKX2.1EGFP
KNOCKIN
REPORTER
LINE
38
5.1.1.
TARGETED
INTEGRATION
OF
THE
NKX2.1
TARGETING
VECTOR
INTO
THE
NKX2.1
LOCUS
38
5.1.2.
CHARACTERIZATION
OF
THE
HIPSC-NKX2.1EGFP
REPORTER
LINE
41
5.1.3.
DIRECTED
DIFFERENTIATION
OF
THE
HIPSC-NKX2.1EGFP
LINE
INTO
LUNG
EPITHELIAL
PROGENITOR
CELLS
.
43
5.2.
GENERATION
OF
THE
HIPSC-NKX2.1EGFP-CCSPMVENUS
NU
C
KNOCKIN
REPORTER
LINE
49
5.2.1.
TARGETED
INTEGRATION
OF
THE
CCSP
TARGETING
VECTOR
INTO
THE
CCSP
LOCUS
49
5.2.2.
CHARACTERIZATION
OF
THE
HIPSC-NKX2.1EGFP-CCSPMVENUS
NU
C
REPORTER
LINES
50
5.3.
GENERATION
OF
THE
HIPSC-NKX2.1EGFP-P63MVENUS
NU
C
KNOCKIN
REPORTER
LINE
53
5.3.1.
TARGETED
INTEGRATION
OF
THE
P63
TARGETING
VECTOR
INTO
THE
P63
LOCUS
53
5.3.2.
CHARACTERIZATION
OF
THE
HIPSC-NKX2.1EGFP-P63MVENUS
NU
C
REPORTER
LINE
56
5.3.3.
DIRECTED
DIFFERENTIATION
OF
THE
HIPSC-NKX2.1EGFP-P63MVENUS
NU
C
REPORTER
LINE
58
5.3.4.
ANTIBIOTIC
PURIFICATION
OF
P63MVENUS
N
UC
P0S
CELLS
63
5.3.5.
FURTHER
MATURATION
OF
P63MVENUS
N
UC
P0S
G418-SELECTED
CELLS
UNDER
AIR
LIQUID-INTERFACE
CONDITIONS
66
6.
DISCUSSION
74
6.1.
TALEN-BASED
GENERATION
OF
FUNCTIONAL
HIPSC
KNOCKIN
REPORTER
LINES
74
6.2.
DIFFERENTIATION
POTENTIAL
OF
ESTABLISHED
HIPSC
KNOCKIN
REPORTER
LINES
77
6.3.
PURIFICATION
OF
POTENTIAL
AIRWAY
BASAL
CELLS
BY
USE
OF
THE
HIPSC-NKX2.1EGFP-
P63MVENUS
NU
C
REPORTER
LINE
.................................................................................
80
7.
CONCLUSION
AND
OUTLOOK
............................................................................................84
8.
REFERENCES
....................................................................................................................86
9.
APPENDIX
.......................................................................................................................96
9.1.
SUPPLEMENTAL
INFORMATIONS
...................................................................................
96
9.2.
LIST
OF
FIGURES
AND
TABLES
......................................................................................100
9.3.
LIST
OF
ABBREVIATIONS
.............................................................................................
101
9.4.
VIDEO
OF
ALI
CULTURES
............................................................................................104
10.
CURRICULUM
VITAE
........................................................
105
11.
AUTHOR S
STATEMENT
OF
CONTRIBUTION
.....................................................................
107
12.
DECLARATION
................................................................................................................
108
VI
|
| adam_txt |
TABLE
OF
CONTENT
ABSTRACT
.
1
1.
INTRODUCTION
.
2
1.1.
THE
RESPIRATORY
SYSTEM
.
2
1.1.1.
LUNG
DEVELOPMENT
.
4
1.1.2.
CYSTIC
FIBROSIS
.
5
1.1.3.
THERAPEUTIC
OPTIONS
FOR
CYSTIC
FIBROSIS
.
6
1.2.
HUMAN
INDUCED
PLURIPOTENT
STEM
CELLS
IN
REGENERATIVE
MEDICINE
.
7
1.2.1.
DIRECTED
DIFFERENTIATION
OF
HIPSCS
INTO
LUNG
EPITHELIAL
CELLS
.
9
1.3.
METHODS
FOR
GENERATION
OF
REPORTER
CELL
LINES
.
11
1.3.1.
KNOCKIN
HUMAN
PLURIPOTENT
STEM
CELL
REPORTER
LINES
IN
REGENERATIVE
MEDICINE
.
13
2.
AIMS
OF
THE
STUDY
.
15
3.
MATERIAL
.
16
3.1.
CHEMICALS
.
16
3.2.
PRIMARY
ANTIBODIES
.
17
3.3.
ISOTYPE
CONTROLS
.
19
3.4.
SECONDARY
ANTIBODIES
.
19
3.5.
CULTURE
MEDIA
.
20
3.6.
BUFFERS
.
21
3.7.
VECTORS
&
TALENS
.
22
3.7.1.
NKX2.1
TARGETING
VECTOR
.
22
3.7.2.
P63
TARGETING
VECTOR
.
23
3.7.3.
CCSP
TARGETING
VECTOR
.
24
3.7.4.
FLP
RECOMBINASE
EXPRESSING
VECTOR
.
25
3.7.5.
TALENS
.
26
3.8.
BACTERIA
.
27
3.9.
PRIMER
.
27
3.10.
CELL
LINES
.
29
3.10.1.
MOUSE
EMBRYONIC
FIBROBLASTS
.
29
3.10.2.
HHSC_F1285_T-IPS2
.
29
4.
METHODS
.
30
IV
4.1.
CELL
CULTURE
30
4.1.1.
MOUSE
EMBRYONIC
FIBROBLASTS
(MEFS)
30
4.1.2.
CULTIVATION
OF
UNDIFFERENTIATED
HIPSCS
30
4.2.
DIRECTED
DIFFERENTIATION
OF
HIPSCS
31
4.3.
FLOW
CYTOMETRY
STAINING
33
4.4.
IMMUNOFLUORESCENCE
STAINING
34
4.5.
REPORTER
CELL
LINE
GENERATION
35
4.6.
UNDIRECTED
DIFFERENTIATION
OF
HIPSCS
INTO
DERIVATIVES
OF
ALL
THREE
GERM
LAYERS
35
4.7.
KARYOTYPE
ANALYSIS
36
4.8.
MRNA
EXPRESSION
ANALYSIS
36
4.9.
TRANSMISSION
ELECTRON
MICROSCOPY
37
4.10.
STATISTICAL
ANALYSIS
37
5.
RESULTS
38
5.1.
GENERATION
OF
A
HIPSC-NKX2.1EGFP
KNOCKIN
REPORTER
LINE
38
5.1.1.
TARGETED
INTEGRATION
OF
THE
NKX2.1
TARGETING
VECTOR
INTO
THE
NKX2.1
LOCUS
38
5.1.2.
CHARACTERIZATION
OF
THE
HIPSC-NKX2.1EGFP
REPORTER
LINE
41
5.1.3.
DIRECTED
DIFFERENTIATION
OF
THE
HIPSC-NKX2.1EGFP
LINE
INTO
LUNG
EPITHELIAL
PROGENITOR
CELLS
.
43
5.2.
GENERATION
OF
THE
HIPSC-NKX2.1EGFP-CCSPMVENUS
NU
C
KNOCKIN
REPORTER
LINE
49
5.2.1.
TARGETED
INTEGRATION
OF
THE
CCSP
TARGETING
VECTOR
INTO
THE
CCSP
LOCUS
49
5.2.2.
CHARACTERIZATION
OF
THE
HIPSC-NKX2.1EGFP-CCSPMVENUS
NU
C
REPORTER
LINES
50
5.3.
GENERATION
OF
THE
HIPSC-NKX2.1EGFP-P63MVENUS
NU
C
KNOCKIN
REPORTER
LINE
53
5.3.1.
TARGETED
INTEGRATION
OF
THE
P63
TARGETING
VECTOR
INTO
THE
P63
LOCUS
53
5.3.2.
CHARACTERIZATION
OF
THE
HIPSC-NKX2.1EGFP-P63MVENUS
NU
C
REPORTER
LINE
56
5.3.3.
DIRECTED
DIFFERENTIATION
OF
THE
HIPSC-NKX2.1EGFP-P63MVENUS
NU
C
REPORTER
LINE
58
5.3.4.
ANTIBIOTIC
PURIFICATION
OF
P63MVENUS
N
UC
P0S
CELLS
63
5.3.5.
FURTHER
MATURATION
OF
P63MVENUS
N
UC
P0S
G418-SELECTED
CELLS
UNDER
AIR
LIQUID-INTERFACE
CONDITIONS
66
6.
DISCUSSION
74
6.1.
TALEN-BASED
GENERATION
OF
FUNCTIONAL
HIPSC
KNOCKIN
REPORTER
LINES
74
6.2.
DIFFERENTIATION
POTENTIAL
OF
ESTABLISHED
HIPSC
KNOCKIN
REPORTER
LINES
77
6.3.
PURIFICATION
OF
POTENTIAL
AIRWAY
BASAL
CELLS
BY
USE
OF
THE
HIPSC-NKX2.1EGFP-
P63MVENUS
NU
C
REPORTER
LINE
.
80
7.
CONCLUSION
AND
OUTLOOK
.84
8.
REFERENCES
.86
9.
APPENDIX
.96
9.1.
SUPPLEMENTAL
INFORMATIONS
.
96
9.2.
LIST
OF
FIGURES
AND
TABLES
.100
9.3.
LIST
OF
ABBREVIATIONS
.
101
9.4.
VIDEO
OF
ALI
CULTURES
.104
10.
CURRICULUM
VITAE
.
105
11.
AUTHOR'S
STATEMENT
OF
CONTRIBUTION
.
107
12.
DECLARATION
.
108
VI |
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| id | DE-604.BV046708280 |
| illustrated | Illustrated |
| index_date | 2024-07-03T14:30:29Z |
| indexdate | 2024-07-10T08:51:42Z |
| institution | BVB |
| isbn | 9781912186099 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032118719 |
| oclc_num | 1190676443 |
| open_access_boolean | |
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| physical | 275 Seiten Illustrationen 24 cm |
| psigel | UBM-RCC |
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| publisher | The White Horse Press |
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| spelling | Quigley, Peter 1951- Verfasser aut The forbidden subject how oppositional aesthetics banished natural beauty from the arts Peter Quigley Cambridgeshire, UK The White Horse Press 2019 275 Seiten Illustrationen 24 cm txt rdacontent sti rdacontent n rdamedia nc rdacarrier Nature (Aesthetics) DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032118719&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Quigley, Peter 1951- The forbidden subject how oppositional aesthetics banished natural beauty from the arts |
| title | The forbidden subject how oppositional aesthetics banished natural beauty from the arts |
| title_auth | The forbidden subject how oppositional aesthetics banished natural beauty from the arts |
| title_exact_search | The forbidden subject how oppositional aesthetics banished natural beauty from the arts |
| title_exact_search_txtP | The forbidden subject how oppositional aesthetics banished natural beauty from the arts |
| title_full | The forbidden subject how oppositional aesthetics banished natural beauty from the arts Peter Quigley |
| title_fullStr | The forbidden subject how oppositional aesthetics banished natural beauty from the arts Peter Quigley |
| title_full_unstemmed | The forbidden subject how oppositional aesthetics banished natural beauty from the arts Peter Quigley |
| title_short | The forbidden subject |
| title_sort | the forbidden subject how oppositional aesthetics banished natural beauty from the arts |
| title_sub | how oppositional aesthetics banished natural beauty from the arts |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032118719&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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