The Plant Cell Wall: Methods and Protocols

This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and struc...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Format: Buch
Sprache:English
Veröffentlicht: New York, NY Humana Press [2020]
Ausgabe:Second Edition
Schriftenreihe:Methods in Molecular Biology 2149
Schlagworte:
Zusammenfassung:This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field
Beschreibung:Preface…Acknowledgments…Table of Contents…Contributing Authors…; 1. High-Voltage Paper Electrophoresis (HVPE)Stephen C. Fry; 2. Carbohydrate Gel ElectrophoresisFlorence Goubet, Paul Dupree, and Katja Salomon Johansen; 3. Capillary Electrophoresis with Detection by Laser-Induced FluorescenceAndrew Mort and Xiangmei Wu; 4. Theory and Practice in Measuring In Vitro Extensibility of Growing Plant Cell WallsDaniel J. Cosgrove; 5. Formation of Cellulose-Based Composites with Hemicelluloses and Pectins using Komagataeibacter FermentationDeirdre Mikkelsen, Patricia Lopez-Sanchez, Dongjie Wang, and Michael J. Gidley ; 6. Plant Tissue CulturesAnna Kärkönen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda; 7. Protoplast Isolation and Manipulation in the Unicellular Model Plant, Penium margaritaceumDavid S. Domozych, Eleanore Ritter, Anna Lietz, Berke Tinaz, and Sandra C. Raimundo; 8. Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patensAlison W.
- Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, Virginia Lai, and Arielle M. Chaves; 9. Expression of Cell-Wall Modifying Enzymes in Aspen for Improved Lignocellulose ProcessingMarta Derba-Maceluch and Ewa J. Mellerowicz; 10. Activity and Action of Cell-Wall TransglycanasesLenka Franková and Stephen C. Fry; 11. Screening for Cellulolytic Plant Enzymes using Colorimetric and Fluorescence MethodsKirsten Krause and Stian Olsen; 12. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell WallsStefan J. Hill, Tracey J. Bootten, Laurence D. Melton, and Philip J. Harris; 13. High-Resolution Imaging of Cellulose Organization in Cell Walls by Field Emission Scanning Electron MicroscopyYunzhen Zheng, Gang Ning, and Daniel J. Cosgrove; 14. Analyzing Plant Cell Wall Ultrastructure by Scanning Near-Field Optical Microscopy (SNOM)Tobias Keplinger and Ingo Burgert; 15.
- Raman Imaging of Plant Cell WallsBatirtze Prats Mateu, Peter Bock, and Notburga Gierlinger; 16. Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared SpectroscopyRicardo M. F. da Costa, William Barrett, José Carli, and Gordon G. Allison; 17. Rapid Assessment of Cell Wall Polymer Distribution and Surface Topology of Arabidopsis SeedlingsMary L. Tierney, Li Sun, and David S. Domozych; 18. Analysis of Plant Cell Walls using High-Throughput Profiling Techniques with Multivariate MethodsJohn P. Moore, Yu Gao, Anscha J.J. Zietsman, Jonatan U. Fangel, Johan Trygg, William G.T. Willats, and Melané A. Vivier; 19. Vibratome Sectioning of Plant Materials for (Immuno)Cytochemical StainingOlivier Leroux; 20. Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Cell Walls from Plants and Marine AlgaeDelphine Duffieux, Susan E. Marcus, J. Paul Knox, and Cécile Hervé; 21.
- Electron Tomography and Immunogold Labeling as Tools to Analyze de novo Assembly of Plant Cell WallsMarisa S. Otegui; 22. Contributions to Arabinogalactan Proteins AnalysisRomain Castilleux, Marc Ropitaux, Youssef Manasfi, Sophie Bernard, Maïté Vicré-Gibouin, and Azeddine Driouich; 23. In Situ/Subcellular Localization of Arabinogalactan Protein Expression by Fluorescent In Situ Hybridization, FISHMário Luís da Costa, María-Teresa Solís, Pilar S. Testillano, and Sílvia Coimbra; 24. Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan ProteinsReina J. Veenhof and Zoë A. Popper; 25. Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of ProteinsDavid Roujol, Laurent Hoffmann, Hélène San Clemente, Corinne Schmitt-Keichinger, Christophe Ritzenthaler, Vincent Burlat, and Elisabeth Jamet; 26.
- Bioinformatic Identification of Plant Hydroxyproline-Rich GlycoproteinsXiao Liu, Savannah McKenna, Lonnie R. Welch, and Allan M. Showalter; 27. Bioinformatics Analysis of Plant Cell Wall EvolutionElisabeth Fitzek, Rhiannon Balazic, and Yanbin Yin; 28. Automated Glycan Assembly of Plant Cell Wall OligosaccharidesFabian Pfrengle; 29. Computerized Molecular Modelling of CarbohydratesAlfred D. French and Glenn P. Johnson
Beschreibung:xvii, 544 Seiten Illustrationen, Diagramme 254 mm
ISBN:9781071606193

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