Mitochondria:
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA ; San Diego, CA
Academic Press, an imprint of Elsevier
2020
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| Ausgabe: | 3rd edition |
| Schriftenreihe: | Methods in cell biology
Volume 155 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xxiv, 579 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128202289 |
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| adam_text | Contents Contributors........................................................................................................... xv Preface................................................................................................................ xxiii SECTION I CHAPTER 1 Isolation of mitochondria and analysis of mitochondrial compartments Isolation of mitochondria from cells andtissues......... з Pin-Chao Liao, Christian Bergamini, Romana Fato, Liza A. Pon, Francesco Pallotti 1. 2. 3. 4. 5. 6. Isolation of mitochondria from rat brain....................................... 5 Isolation of mitochondria from rat liver...................................... 10 Isolation of mitochondria from beef heart................................... 13 Isolation of mitochondria from skeletal muscle.......................... 16 Isolation of mitochondria from cultured cells............................. 18 Affinity purification of mitochondria from yeast using magnetic beads............................................................................. 21 Acknowledgments..............................................................................27 References..........................................................................................27 CHAPTER 2 Isolation of mitochondria-associated ER membranes........................................................................ зз Jorge Montesinos, Estela Area-Gomez 1. Introduction.................................................................................. 34 2.
Rationale.......................................................................................34 3. Isolation of MAM domains..........................................................35 4. Precursor techniques.................................................................... 41 5. Analysis and statistics.................................................................. 41 6. Pros and cons................................................................................42 7. Troubleshooting and optimization............................................... 42 8. Summary...................................................................................... 42 Acknowledgments............................................................................. 43 References..........................................................................................43 CHAPTER 3 Studying protein importintomitochondria................... 45 Chantal Priesnitz, Nikolaus Pfanner, Thomas Becker 1. 2. 3. 4. Rationale.......................................................................................47 Cell-free synthesis of radiolabeled precursor proteins................49 Protein import into mitochondria.................................................53 Localization studies of imported proteins....................................60 v
vi Contents 5. Analysis of protein complex assembly by blue native electrophoresis.............................................................................. 64 6. Related techniques........................................................................65 7. Crosslinking of imported proteins................................................68 8. Accumulation of import intermediates........................................ 69 9. Disulfide bond formation of imported proteins...........................70 10. Import of chemical amounts of precursor proteins into mitochondria................................................................................. 71 11. Concluding remarks..................................................................... 72 Acknowledgments..............................................................................73 References..........................................................................................73 SECTION II Assays for mitochondrialmetabolic activities CHAPTER 4 Biochemical assays of TCA cycle and ß-oxidation metabolites........................................................................ 83 Michael 1. Bennett, Feng Sheng, Ann Saada 1. 2. 3. 4. 5. 6. Definition......................................................................................85 Metabolite measurements.............................................................91 Fatty acid enzyme and transporterassays...................................95 Fatty acid oxidation metabolic flux studies............................... 102 Fatty acid metabolite accumulation
assays................................ 104 Radiometric assays of pymvate dehydrogenase and a-ketoglutarate dehydrogenase............................................ 105 7. Spectrophotometric assays of TCA cycle enzymes...................108 References........................................................................................ 116 Further reading.................................................................................120 CHAPTER 5 Assessment of mitochondrial respiratory chain enzymes in cells and tissues....................................... 121 Ann E. Frazier, Amy E. Vincent, Doug M. Turnbull, David R. Thorburn, Robert W. Taylor 1. 2. 3. 4. 5. 6. 7. Rationale..................................................................................... 123 Sample preparation..................................................................... 125 Respiratory chain enzymology...................................................130 Protocols..................................................................................... 133 Adaption of methods to other tissues and organisms................ 145 Alternate methods to assay respiratory chain enzymes.............146 Quantitative immunofluorescence..............................................148
Contents 8. Safety considerations and standards.......................................... 151 9. Summary.....................................................................................152 Acknowledgments........................................................................... 152 References........................................................................................152 CHAPTER 6 Assessing mitochondrial respiratory bioenergetics in whole cells and isolated organelles by microplate respirometry.................... 157 Rishi R. Agrawal, Kirstin A. Tamucci, Marta Pera, Delfina Larrea 1. Definition....................................................................................158 2. Rationale.....................................................................................158 3. Materials, equipment, and reagents........................................... 161 4. Protocols..................................................................................... 163 5. Isolated mitochondria.................................................................168 6. Permeabilized cells.....................................................................173 7. Safety considerations and standards.......................................... 174 8. Examples and discussion............................................................174 9. Troubleshooting and optimization............................................. 178 Acknowledgments........................................................................... 179
References........................................................................................179 CHAPTER 7 Analyzing electron transport chain supercomplexes.........................................................ա Rebeca Acín-Pérez, Pablo Hernansanz-Agustín, José Antonio Enriquez 1. Definition....................................................................................182 2. Rationale.....................................................................................182 3. Materials, equipment and reagents............................................ 183 4. Protocols..................................................................................... 186 5. Precursor techniques...................................................................194 6. Safety considerations and standards.......................................... 195 7. Analysis and statistics................................................................ 195 8. Pros and cons..............................................................................195 9. Alternative methods/procedures.................................................195 10. Troubleshooting and optimization............................................. 196 References........................................................................................197 Further reading.................................................................................197 VII
Contents CHAPTER 8 Measurement of ATP concentrations in mitochondria of living cells using luminescence and fluorescence approaches......................................................................199 Giampaolo Morciano, Hiromi Imamura, Simone Patergnani, Gaia Pedriali, Carlona Giorgi, Paolo Pintón 1. The luciferase assay to measure ATP levels............................. 200 2. Visualization of ATP with subcellular resolution using the fluorescent biosensor, ATeam....................................................209 Acknowledgments............................................................................ 217 References........................................................................................ 217 CHAPTER 9 Visualization of mitochondrial membrane potential in mammalian cells.......................................221 Noemi Esteras, Merel J.W. Adjobo-Hermans, Andrey Y. Abramov, Werner J.H. Koopman 1. Definition...................................................................................223 2. Rationale..................................................................................... 223 3. Materials, equipment and reagents.............................................230 4. Protocols......................................................................................233 5. Safety considerations and standards.......................................... 238 6. Analysis and statistics.................................................................238 7. Troubleshooting and optimization............................................. 241
Acknowledgments............................................................................ 242 Potential conflict of interest............................................................ 243 References........................................................................................ 243 Further reading................................................................................. 245 CHAPTER 10 Histochemical and immunohistochemical staining methods to visualize mitochondrial proteins and activity...................................................... 247 Sandra Franco-lborra, Kūrenai Tanji 1. Histochemistry to visualize mitochondria and mitochondrial SDH and COX activity.......................................249 2. Immunohistochemistry to visualize mitochondria.....................256 Acknowledgments............................................................................267 References........................................................................................ 267
Contents SECTION HI Assays for other mitochondrial activities CHAPTER 11 Measurement of mitochondrial H202 production under varying G2 tensions............................................ 273 Anna Stepanova, Alexander Galkin 1. Definition........................................................................................ 274 2. Rationale..........................................................................................274 3. Materials, equipment and reagents...............................................276 4. Protocol........................................................................................... 278 5. Analysis and statistics....................................................................281 6. Precursor techniques...................................................................... 286 7. Safety considerations and standards.............................................287 8. Pros and cons.................................................................................. 287 9. Alternative methods/procedures................................................... 288 10. Troubleshooting and optimization................................................289 11. Summary......................................................................................... 290 References.............................................................................................290 CHAPTER 12 Live cell imaging of mitochondrial redox state in mammalian cells and yeast.....................................295 Pin-Chao Liao, Sandra Franco-lborra, Yi Yang, Liza A. Pon 1.
Rationale..........................................................................................296 2. Visualization of mitochondrial ROS in C2C12myotubes.........297 3. Visualization of mitochondrial superoxide in yeast................... 302 4. Visualization of mitochondrial NAD PH in mammalian cells using iNap sensors..........................................................................306 5. Use of genetically encoded biosensors to detect mitochondrial redox state in yeast................................................312 Acknowledgments................................................................................ 316 References.............................................................................................317 CHAPTER 13 Analysis of phospholipid synthesis in mitochondria................................................................... 321 Jorge Montesinos, Estela Area-Gomez, Michael Schlame 1. Introduction..................................................................................... 322 2. Mitochondrial biosynthesis of phosphatidylethanolamine....... 322 3. Mitochondrial biosynthesis of cardiolipin................................... 327 References.............................................................................................333 IX
x Contents CHAPTER 14 Biosensors for detection of calcium........................ 337 Elisa Greotti, Diego De Stefani 1. Rationale..................................................................................... 340 2. Materials, equipment and reagents.............................................346 3. Protocols..................................................................................... 351 4. Precursor techniques...................................................................354 5. Safety considerations and standards...........................................354 6. Analysis and statistics................................................................ 354 7. Related techniques......................................................................356 8. Pros and cons.............................................................................. 357 9. Alternative methods/procedures................................................. 357 10. Troubleshooting and optimization..............................................359 11. Summary..................................................................................... 361 References........................................................................................ 361 CHAPTER 15 Measurement of membrane permeability and the mitochondrial permeability transition......... 369 Michela Carraro, Paolo Bernardi 1. Rationale..................................................................................... 370 2. Materials, equipment and reagents.............................................371 3.
Protocol....................................................................................... 372 4. Analysis and statistics................................................................ 376 References........................................................................................ 377 SECTION IV Mitochondrial genes and gene expression CHAPTER 16 Detection of mitochondrial DNA (mtDNA) mutations......................................................................383 Ali Naini, Robert Gilkerson, Sara Shanske, Jiuhong Pang 1. Introduction.................................................................................384 2. MtDNA mutations...................................................................... 386 3. Detecting mtDNA large-scale single deletions by Southern blot analysis........................................................... 387 4. Detecting mtDNA large-scale single deletions by fluorescence in situ hybridization......................................... 387 5. Detecting mtDNA point mutations by PCR/RFLP analysis.... 390 6. Detecting mitochondrial DNA point mutations, deletions and rearrangements by next generation sequencing (NGS).......................................................................390
Contents Acknowledgment.............................................................................399 References....................................................................................... 399 CHAPTER 17 Single-molecule analysis of mtDNA replication With high resolution.......................................................401 Marco Tigano, Aaron Fraser Phillips, Agnei Sfeir 1. Definition................................................................................... 402 2. Materials, equipment and reagents............................................402 3. Protocols.................................................................................... 405 4. Precursor techniques.................................................................. 410 5. Safety considerations and standards..........................................410 6. Analysis and statistics................................................................410 Acknowledgments........................................................................... 414 Competing interests.........................................................................414 References....................................................................................... 414 CHAPTER 18 Cybrid technology.......................................................... 415 Sandra R. Bacman, Nadee Nissanka, Carlos T. Moraes 1. Rationale.................................................................................... 416 2. Generation of p° cells as nuclear donors for cybrids................ 417 3. Generation of mitochondrial
donors..........................................425 4. Generation of cybrids.................................................................430 Acknowledgments........................................................................... 434 References....................................................................................... 434 Further reading................................................................................ 439 CHAPTER 19 Manipulation of mitochondrial genes and mtDNA heteroplasmy...................................................................441 Sandra R. Bacman, P.A. Gammage, M. Minczuk, Carlos T. Moraes 1. Introduction................................................................................ 442 2. Targeting mtDNA with restriction endonucleases.................... 445 3. Targeting mtDNA with mitoTALENs.......................................451 4. Targeting mtDNA with mtZFNs............................................... 462 5. Discussion.................................................................................. 473 6. Concluding remarks................................................................... 477 Acknowledgments........................................................................... 478 References....................................................................................... 478 XI
xii Contents SECTION V Assays for mitochondrial dynamics________ CHAPTER 20 In vitro and in vivo assays for mitochondrial fission and fusion........................................................... 491 Suzanne Hoppins, Laura L. Lackner, Jason E. Lee, Jason A. Mears 1. Rationale..................................................................................... 492 2. Analysis of mitochondrial fusion in vitro..................................494 3. Analysis of mitochondrial fission in vitro................................. 499 4. Analysis of mitochondrial dynamics in vivo............................. 505 Acknowledgments............................................................................ 516 References........................................................................................ 516 CHAPTER 21 Live-cell imaging of mitochondrial motility and interactions in Drosophila neurons and yeast.......................................................................... 519 Pin-Chao Liao, Ryo Higuchi-Sanabria, Theresa C. Swayne, Cierra N. Sing, Liza A. Pon 1. Visualization of mitochondrial trafficking in neurons of Drosophila larvae...................................................................520 2. Multi-color imaging of mitochondria and other organelles in S. cerevisiae............................................................................ 527 3. Visualization and quantitative analysis of mitochondrial motility and distribution in living yeast cells............................ 536
References........................................................................................ 542 CHAPTER 22 Method for live-cell super-resolution imaging of mitochondrial cristae and quantification of submitochondrial membrane potentials................ 545 Dane M. Wolf, Mayuko Segawa, Orian S. Shirihai, Marc Liesa 1. Rationale..................................................................................... 546 2. Materials, equipment and reagents.............................................546 3. Protocols......................................................................................547 4. Safety considerations and standards...........................................549 5. Analysis and statistics................................................................ 549 6. Pros and cons.............................................................................. 553 7. Troubleshooting and optimization............................................. 553 8. Summary..................................................................................... 553 Acknowledgments............................................................................ 554 Conflict of interest........................................................................... 554 References........................................................................................ 554
Contents CHAPTER 23 Detection of mitophagy in mammalian cells, mice, and yeast.............................................................. 557 Hannah Calvelli, Judith Krigman, Mashun Onishi, Derek P. Narendra, Nuo Sun, Koji Okamoto 1. Analysis of mitophagy in cultured mammalian cells................558 2. Analysis of mitophagy in mice..................................................566 3. Analysis of mitophagy in yeast................................................. 569 4. Summary.................................................................................... 576 Acknowledgments........................................................................... 576 References....................................................................................... 577 xiii
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| adam_txt |
Contents Contributors. xv Preface. xxiii SECTION I CHAPTER 1 Isolation of mitochondria and analysis of mitochondrial compartments Isolation of mitochondria from cells andtissues. з Pin-Chao Liao, Christian Bergamini, Romana Fato, Liza A. Pon, Francesco Pallotti 1. 2. 3. 4. 5. 6. Isolation of mitochondria from rat brain. 5 Isolation of mitochondria from rat liver. 10 Isolation of mitochondria from beef heart. 13 Isolation of mitochondria from skeletal muscle. 16 Isolation of mitochondria from cultured cells. 18 Affinity purification of mitochondria from yeast using magnetic beads. 21 Acknowledgments.27 References.27 CHAPTER 2 Isolation of mitochondria-associated ER membranes. зз Jorge Montesinos, Estela Area-Gomez 1. Introduction. 34 2.
Rationale.34 3. Isolation of MAM domains.35 4. Precursor techniques. 41 5. Analysis and statistics. 41 6. Pros and cons.42 7. Troubleshooting and optimization. 42 8. Summary. 42 Acknowledgments. 43 References.43 CHAPTER 3 Studying protein importintomitochondria. 45 Chantal Priesnitz, Nikolaus Pfanner, Thomas Becker 1. 2. 3. 4. Rationale.47 Cell-free synthesis of radiolabeled precursor proteins.49 Protein import into mitochondria.53 Localization studies of imported proteins.60 v
vi Contents 5. Analysis of protein complex assembly by blue native electrophoresis. 64 6. Related techniques.65 7. Crosslinking of imported proteins.68 8. Accumulation of import intermediates. 69 9. Disulfide bond formation of imported proteins.70 10. Import of chemical amounts of precursor proteins into mitochondria. 71 11. Concluding remarks. 72 Acknowledgments.73 References.73 SECTION II Assays for mitochondrialmetabolic activities CHAPTER 4 Biochemical assays of TCA cycle and ß-oxidation metabolites. 83 Michael 1. Bennett, Feng Sheng, Ann Saada 1. 2. 3. 4. 5. 6. Definition.85 Metabolite measurements.91 Fatty acid enzyme and transporterassays.95 Fatty acid oxidation metabolic flux studies. 102 Fatty acid metabolite accumulation
assays. 104 Radiometric assays of pymvate dehydrogenase and a-ketoglutarate dehydrogenase. 105 7. Spectrophotometric assays of TCA cycle enzymes.108 References. 116 Further reading.120 CHAPTER 5 Assessment of mitochondrial respiratory chain enzymes in cells and tissues. 121 Ann E. Frazier, Amy E. Vincent, Doug M. Turnbull, David R. Thorburn, Robert W. Taylor 1. 2. 3. 4. 5. 6. 7. Rationale. 123 Sample preparation. 125 Respiratory chain enzymology.130 Protocols. 133 Adaption of methods to other tissues and organisms. 145 Alternate methods to assay respiratory chain enzymes.146 Quantitative immunofluorescence.148
Contents 8. Safety considerations and standards. 151 9. Summary.152 Acknowledgments. 152 References.152 CHAPTER 6 Assessing mitochondrial respiratory bioenergetics in whole cells and isolated organelles by microplate respirometry. 157 Rishi R. Agrawal, Kirstin A. Tamucci, Marta Pera, Delfina Larrea 1. Definition.158 2. Rationale.158 3. Materials, equipment, and reagents. 161 4. Protocols. 163 5. Isolated mitochondria.168 6. Permeabilized cells.173 7. Safety considerations and standards. 174 8. Examples and discussion.174 9. Troubleshooting and optimization. 178 Acknowledgments. 179
References.179 CHAPTER 7 Analyzing electron transport chain supercomplexes.ա Rebeca Acín-Pérez, Pablo Hernansanz-Agustín, José Antonio Enriquez 1. Definition.182 2. Rationale.182 3. Materials, equipment and reagents. 183 4. Protocols. 186 5. Precursor techniques.194 6. Safety considerations and standards. 195 7. Analysis and statistics. 195 8. Pros and cons.195 9. Alternative methods/procedures.195 10. Troubleshooting and optimization. 196 References.197 Further reading.197 VII
Contents CHAPTER 8 Measurement of ATP concentrations in mitochondria of living cells using luminescence and fluorescence approaches.199 Giampaolo Morciano, Hiromi Imamura, Simone Patergnani, Gaia Pedriali, Carlona Giorgi, Paolo Pintón 1. The luciferase assay to measure ATP levels. 200 2. Visualization of ATP with subcellular resolution using the fluorescent biosensor, ATeam.209 Acknowledgments. 217 References. 217 CHAPTER 9 Visualization of mitochondrial membrane potential in mammalian cells.221 Noemi Esteras, Merel J.W. Adjobo-Hermans, Andrey Y. Abramov, Werner J.H. Koopman 1. Definition.223 2. Rationale. 223 3. Materials, equipment and reagents.230 4. Protocols.233 5. Safety considerations and standards. 238 6. Analysis and statistics.238 7. Troubleshooting and optimization. 241
Acknowledgments. 242 Potential conflict of interest. 243 References. 243 Further reading. 245 CHAPTER 10 Histochemical and immunohistochemical staining methods to visualize mitochondrial proteins and activity. 247 Sandra Franco-lborra, Kūrenai Tanji 1. Histochemistry to visualize mitochondria and mitochondrial SDH and COX activity.249 2. Immunohistochemistry to visualize mitochondria.256 Acknowledgments.267 References. 267
Contents SECTION HI Assays for other mitochondrial activities CHAPTER 11 Measurement of mitochondrial H202 production under varying G2 tensions. 273 Anna Stepanova, Alexander Galkin 1. Definition. 274 2. Rationale.274 3. Materials, equipment and reagents.276 4. Protocol. 278 5. Analysis and statistics.281 6. Precursor techniques. 286 7. Safety considerations and standards.287 8. Pros and cons. 287 9. Alternative methods/procedures. 288 10. Troubleshooting and optimization.289 11. Summary. 290 References.290 CHAPTER 12 Live cell imaging of mitochondrial redox state in mammalian cells and yeast.295 Pin-Chao Liao, Sandra Franco-lborra, Yi Yang, Liza A. Pon 1.
Rationale.296 2. Visualization of mitochondrial ROS in C2C12myotubes.297 3. Visualization of mitochondrial superoxide in yeast. 302 4. Visualization of mitochondrial NAD PH in mammalian cells using iNap sensors.306 5. Use of genetically encoded biosensors to detect mitochondrial redox state in yeast.312 Acknowledgments. 316 References.317 CHAPTER 13 Analysis of phospholipid synthesis in mitochondria. 321 Jorge Montesinos, Estela Area-Gomez, Michael Schlame 1. Introduction. 322 2. Mitochondrial biosynthesis of phosphatidylethanolamine. 322 3. Mitochondrial biosynthesis of cardiolipin. 327 References.333 IX
x Contents CHAPTER 14 Biosensors for detection of calcium. 337 Elisa Greotti, Diego De Stefani 1. Rationale. 340 2. Materials, equipment and reagents.346 3. Protocols. 351 4. Precursor techniques.354 5. Safety considerations and standards.354 6. Analysis and statistics. 354 7. Related techniques.356 8. Pros and cons. 357 9. Alternative methods/procedures. 357 10. Troubleshooting and optimization.359 11. Summary. 361 References. 361 CHAPTER 15 Measurement of membrane permeability and the mitochondrial permeability transition. 369 Michela Carraro, Paolo Bernardi 1. Rationale. 370 2. Materials, equipment and reagents.371 3.
Protocol. 372 4. Analysis and statistics. 376 References. 377 SECTION IV Mitochondrial genes and gene expression CHAPTER 16 Detection of mitochondrial DNA (mtDNA) mutations.383 Ali Naini, Robert Gilkerson, Sara Shanske, Jiuhong Pang 1. Introduction.384 2. MtDNA mutations. 386 3. Detecting mtDNA large-scale single deletions by Southern blot analysis. 387 4. Detecting mtDNA large-scale single deletions by fluorescence in situ hybridization. 387 5. Detecting mtDNA point mutations by PCR/RFLP analysis. 390 6. Detecting mitochondrial DNA point mutations, deletions and rearrangements by next generation sequencing (NGS).390
Contents Acknowledgment.399 References. 399 CHAPTER 17 Single-molecule analysis of mtDNA replication With high resolution.401 Marco Tigano, Aaron Fraser Phillips, Agnei Sfeir 1. Definition. 402 2. Materials, equipment and reagents.402 3. Protocols. 405 4. Precursor techniques. 410 5. Safety considerations and standards.410 6. Analysis and statistics.410 Acknowledgments. 414 Competing interests.414 References. 414 CHAPTER 18 Cybrid technology. 415 Sandra R. Bacman, Nadee Nissanka, Carlos T. Moraes 1. Rationale. 416 2. Generation of p° cells as nuclear donors for cybrids. 417 3. Generation of mitochondrial
donors.425 4. Generation of cybrids.430 Acknowledgments. 434 References. 434 Further reading. 439 CHAPTER 19 Manipulation of mitochondrial genes and mtDNA heteroplasmy.441 Sandra R. Bacman, P.A. Gammage, M. Minczuk, Carlos T. Moraes 1. Introduction. 442 2. Targeting mtDNA with restriction endonucleases. 445 3. Targeting mtDNA with mitoTALENs.451 4. Targeting mtDNA with mtZFNs. 462 5. Discussion. 473 6. Concluding remarks. 477 Acknowledgments. 478 References. 478 XI
xii Contents SECTION V Assays for mitochondrial dynamics_ CHAPTER 20 In vitro and in vivo assays for mitochondrial fission and fusion. 491 Suzanne Hoppins, Laura L. Lackner, Jason E. Lee, Jason A. Mears 1. Rationale. 492 2. Analysis of mitochondrial fusion in vitro.494 3. Analysis of mitochondrial fission in vitro. 499 4. Analysis of mitochondrial dynamics in vivo. 505 Acknowledgments. 516 References. 516 CHAPTER 21 Live-cell imaging of mitochondrial motility and interactions in Drosophila neurons and yeast. 519 Pin-Chao Liao, Ryo Higuchi-Sanabria, Theresa C. Swayne, Cierra N. Sing, Liza A. Pon 1. Visualization of mitochondrial trafficking in neurons of Drosophila larvae.520 2. Multi-color imaging of mitochondria and other organelles in S. cerevisiae. 527 3. Visualization and quantitative analysis of mitochondrial motility and distribution in living yeast cells. 536
References. 542 CHAPTER 22 Method for live-cell super-resolution imaging of mitochondrial cristae and quantification of submitochondrial membrane potentials. 545 Dane M. Wolf, Mayuko Segawa, Orian S. Shirihai, Marc Liesa 1. Rationale. 546 2. Materials, equipment and reagents.546 3. Protocols.547 4. Safety considerations and standards.549 5. Analysis and statistics. 549 6. Pros and cons. 553 7. Troubleshooting and optimization. 553 8. Summary. 553 Acknowledgments. 554 Conflict of interest. 554 References. 554
Contents CHAPTER 23 Detection of mitophagy in mammalian cells, mice, and yeast. 557 Hannah Calvelli, Judith Krigman, Mashun Onishi, Derek P. Narendra, Nuo Sun, Koji Okamoto 1. Analysis of mitophagy in cultured mammalian cells.558 2. Analysis of mitophagy in mice.566 3. Analysis of mitophagy in yeast. 569 4. Summary. 576 Acknowledgments. 576 References. 577 xiii |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV046636858 |
| illustrated | Illustrated |
| index_date | 2024-07-03T14:13:14Z |
| indexdate | 2024-07-10T08:49:54Z |
| institution | BVB |
| isbn | 9780128202289 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032048292 |
| oclc_num | 1150841651 |
| open_access_boolean | |
| owner | DE-703 DE-355 DE-BY-UBR DE-11 |
| owner_facet | DE-703 DE-355 DE-BY-UBR DE-11 |
| physical | xxiv, 579 Seiten Illustrationen, Diagramme |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Academic Press, an imprint of Elsevier |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Mitochondria edited by Liza A. Pon, Eric A. Schon 3rd edition Cambridge, MA ; San Diego, CA Academic Press, an imprint of Elsevier 2020 xxiv, 579 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 155 Mitochondriën gtt Mitochondria Mitochondrium (DE-588)4124939-2 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mitochondrium (DE-588)4124939-2 s Labortechnik (DE-588)4123602-6 s DE-604 Molekularbiologie (DE-588)4039983-7 s Pon, Liza A. (DE-588)1208727826 edt Schon, Eric A. (DE-588)1208728326 edt Erscheint auch als Online-Ausgabe 978-0-12-820229-6 Methods in cell biology Volume 155 (DE-604)BV002534878 155 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032048292&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mitochondria Methods in cell biology Mitochondriën gtt Mitochondria Mitochondrium (DE-588)4124939-2 gnd Molekularbiologie (DE-588)4039983-7 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4124939-2 (DE-588)4039983-7 (DE-588)4123602-6 (DE-588)4143413-4 |
| title | Mitochondria |
| title_auth | Mitochondria |
| title_exact_search | Mitochondria |
| title_exact_search_txtP | Mitochondria |
| title_full | Mitochondria edited by Liza A. Pon, Eric A. Schon |
| title_fullStr | Mitochondria edited by Liza A. Pon, Eric A. Schon |
| title_full_unstemmed | Mitochondria edited by Liza A. Pon, Eric A. Schon |
| title_short | Mitochondria |
| title_sort | mitochondria |
| topic | Mitochondriën gtt Mitochondria Mitochondrium (DE-588)4124939-2 gnd Molekularbiologie (DE-588)4039983-7 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Mitochondriën Mitochondria Mitochondrium Molekularbiologie Labortechnik Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032048292&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT ponlizaa mitochondria AT schonerica mitochondria |