The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Freiburg im Breisgau
[2019?]
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| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 116 Blätter Illustrationen, Diagramme |
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| 100 | 1 | |a Zhang, Pei Pei |d 1989- |e Verfasser |0 (DE-588)1188271709 |4 aut | |
| 245 | 1 | 0 | |a The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization |c vorgelegt 2018 von Peipei Zhang |
| 264 | 1 | |a Freiburg im Breisgau |c [2019?] | |
| 300 | |a 116 Blätter |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Albert-Ludwigs-Universität Freiburg im Breisgau |d 2019 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031836636&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-031836636 | ||
Datensatz im Suchindex
| _version_ | 1804180971647401984 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
DECLARATION
................................................................................................................
3
ABSTRACT
......................................................................................................................
4
ZUSAMMENFASSUNG
...................................................................................................
5
TABLE
OF
CONTENTS
......................................................................................................
6
1.
INTRODUCTION
............................................................................................................
9
1.1.
ANATOMY
OF
THE
EYE
..................................................................................................................
10
1.1.1.
STRUCTURE
OF
THE
RETINA
..........................................................................................................
10
1.1.2.
BLOOD
SUPPLY
OF
THE
RETINA
...................................................................................................
11
1.2.
AGE-RELATED
MACULAR
DEGENERATION
.......................................................................................
13
1.3.
MYELOID
CELLS
..............................................................................................................................
15
1.3.1.
MYELOID
CELLS
IN
THE
HEALTHY
RETINA
.....................................................................................
15
1.3.2.
MYELOID
CELLS
IN
AMD
AND
CNV
...........................................................................................
18
1.4.
INTERFERON
REGULATORY
FACTOR
8
.................................................................................................
20
1.5.
THE
ANIMAL
MODEL
OF
LASER-INDUCED
CNV
..............................................................................
20
1.6.
THE
AIM
OF
THE
PROJECT
..............................................................................................................
21
2.
MATERIALS
AND
METHODS
........................................................................................
23
2.1.
MATERIALS
.....................................................................................................................................
23
2.1.1.
EQUIPMENT
...............................................................................................................................
23
2.1.2.
CONSUMABLES
.........................................................................................................................
24
2.1.3.
MOUSE
DISSECTION
INSTRUMENTS
............................................................................................
24
2.1.4.
CHEMICALS
AND
REAGENTS
.....................................................................................................
25
2.1.5.
BUFFERS
AND
SOLUTIONS
............................................................................................................
26
2.1.6.
ANTIBODIES
FOR
IMMUNOHISTOCHEMISTRY
.................................................................................
27
2.1.7.
ANTIBODIES
FOR
FLOW
CYTOMETRY
AND
FLUORESCENCE-ACTIVATED
CELL
SORTING
.....................
28
2.1.8.
SOFTWARE
..................................................................................................................................
29
2.2.
MOUSE
STRAINS
.........................................................................................................................
29
2.2.1.
CX3CR1
GM+
MICE
.....................................................................................................................
30
6
2.2.2.
CX3CR1
GFPI+
:
LRF&-
MICE
..........................................................................................................
30
2.2.3.
/RF8-VEN
US
REPORTER
MICE
...................................................................................................
30
2.3.
GENOTYPING
................................................................................................................................
31
2.4.
ANESTHESIA
.................................................................................................................................
33
2.5.
PUPILLARY
DILATATION
....................................................................................................................
33
2.6.
INTRACARDIAL
PERFUSION,
BLOOD
ACQUISITION
AND
ENUCLEATION
.................................................
33
2.7.
LASER
TREATMENT
........................................................................................................................
33
2.8.
IN
VIVO
CHARACTERIZATION
............................................................................................................
34
2.8.1.
ELECTRORETINOGRAPHY
...............................................................................................................
34
2.8.2.
OPTICAL
COHERENCE
TOMOGRAPHY
..........................................................................................
35
2.8.3.
FUNDUS
PHOTOGRAPHY
AND
FUNDUS
FLUORESCEIN
ANGIOGRAPHY
..........................................36
2.9.
IMMUNOHISTOCHEMISTRY
.............................................................................................................
36
2.9.1.
IMMUNOHISTOCHEMISTY
OF
WHOLE
FLAT
MOUNTS
.....................................................................
36
2.9.2.
IMMUNOHISTOCHEMISTY
ON
FROZEN
SECTIONS
........................................................................
37
2.9.3.
IMAGING
....................................................................................................................................
38
2.9.4.
ANALYSIS
....................................................................................................................................
39
2.10.
FLOW
CYTOMETRY
AND
FLUORESCENCE-ACTIVATED
CELL
SORTING
..................................................
39
2.10.1.
FLOW
CYTOMETRY
OF
PERIPHERAL
BLOOD
.................................................................................
39
2.10.2.
FLOW
CYTOMETRY
AND
FLUORESCENCE-ACTIVATED
CELL
SORTING
OF
RETINA
............................
40
2.10.3.
DATA
ACQUISITION
...................................................................................................................
41
2.11.
RNA
SEQUENCING
.....................................................................................................................
41
2.11.1.
TOTAL
RNA
ISOLATION
.............................................................................................................
41
2.11.2.
RNA
SEQUENCING
..................................................................................................................
42
2.11.3.
RNA
SEQUENCING
DATA
ANALYSIS
.........................................................................................
42
2.12.
STATISTICAL
ANALYSIS
.................................................................................................................
43
3.
RESULTS
................................................................................................................
44
3.1.
EXPRESSION
PROFILE
OF
THE
IRF
FAMILY
MEMBERS
IN
RETINAL
MICROGLIA
CELLS
.......................
44
3.2.
EXPRESSION
AND
LOCALIZATION
OF
IRF8
IN
RETINAL
CELLS
............................................................
45
3.2.1.
EXPRESSION
PATTERN
OF
IRF8
IN
THE
RETINA
.........................................................................
45
7
3.2.2.
IRF8-VENUS
EXPRESSION
ANALYSIS
IN
CD45
|OW
CD11B
+
RETINAL
MICROGLIA
CELLS
BY
FLOW
CYTOMETRY
..........................................................................................................................................
49
3.3.
THE
IMPACT
OF
IRF8-DEFICIENCY
ON
RETINAL
MICROGLIA
CELLS
.....................................................
51
3.3.1.
IRF8-DEFICIENCY
LEADS
TO
REDUCED
MICROGLIA
CELL
NUMBERS
IN
THE
RETINA
......................
51
3.3.2.
IRF8-DEFICIENCY
LEADS
TO
ABNORMAL
MORPHOLOGY
OF
RETINAL
MICROGLIA
CELLS
....................52
3.4.
IRF8-
DEFICIENCY
DOES
NOT
AFFECT
THE
RETINAL
VASCULATURE
.......................................................
54
3.5.
IRF8-
DEFICIENCY
DOES
NOT
ALTER
THE
RETINAL
ARCHITECTURE
........................................................
56
3.6.
IRF8-DEFICIENCY
DOES
NOT
AFFECT
THE
RETINAL
FUNCTION
.............................................................
57
3.7.
DELETION
OF
IRF8
LEADS
TO
AN
ALTERED
GENE
EXPRESSION
PROFILE
OF
RETINAL
MICROGLIA
CELLS
58
3.7.1.
/RFS-DEFICIENCY
DECREASES
EXPRESSION
OF
MICROGLIA
CELLS
MARKERS
..................................
59
3.7.2.
GENE
ONTOLOGY
ENRICHMENT
ANALYSIS
OF
DIFFERENTIALLY
EXPRESSED
GENES
.....................
61
3.8.
RETINAL
MYELOID
CELLS
AND
LASER-INDUCED
CNV
...................................................................
63
3.9.
THE
ROLE
OF
IRF8
IN
THE
MIGRATION
OF
RETINAL
MICROGLIA
FOLLOWING
LASER
TREATMENT
.......
65
3.10.
THE
EFFECT
OF
IRF8
ON
THE
FORMATION
OF
LASER-INDUCED
CNV
............................................
69
3.10.1.
/RF8-DEFICIENCY
INDUCES
AN
ALTERED
IMMUNE
RESPONSE
IN
RETINAL
MICROGLIA
CELLS
71
3.10.2.
IRF8-
DEFICIENCY
LEADS
TO
A
DEREGULATION
OF
ANGIOGENESIS
............................................
76
4.
DISCUSSION
............................................................................................................
78
4.1.
THE
ROLE
OF
IRF8
IN
RETINAL
MICROGLIA
CELL
HOMEOSTASIS
.....................................................
78
4.2.
THE
IMPACT
OF
IRF8
ON
RETINAL
STRUCTURE
AND
FUNCTION
.......................................................
80
4.3.
IRF8-DEFICIENCY
AND
THE
FORMATION
OF
LASER-INDUCED
CNV
...................................................
82
5.
CONCLUSION
.......................
85
REFERENCES...............................................................................................................
86
LIST
OF
FIGURES
AND
TABLES
.....................................................................................
108
LIST
OF
ABBREVIATIONS
..............................................................................................
109
CURRICULUM
VITAE
....................................................................................................
112
PUBLICATIONS
AND
CONFERENCE
ABSTRACTS
..............................................................
114
ACKNOWLEDGEMENTS
...............................................................................................
115
8
|
| any_adam_object | 1 |
| author | Zhang, Pei Pei 1989- |
| author_GND | (DE-588)1188271709 |
| author_facet | Zhang, Pei Pei 1989- |
| author_role | aut |
| author_sort | Zhang, Pei Pei 1989- |
| author_variant | p p z pp ppz |
| building | Verbundindex |
| bvnumber | BV046424283 |
| ctrlnum | (OCoLC)1141114637 (DE-599)KXP1686060149 |
| discipline | Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV046424283 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:44:14Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031836636 |
| oclc_num | 1141114637 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 116 Blätter Illustrationen, Diagramme |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Zhang, Pei Pei 1989- Verfasser (DE-588)1188271709 aut The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization vorgelegt 2018 von Peipei Zhang Freiburg im Breisgau [2019?] 116 Blätter Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Albert-Ludwigs-Universität Freiburg im Breisgau 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1204266344/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031836636&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zhang, Pei Pei 1989- The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization |
| subject_GND | (DE-588)4113937-9 |
| title | The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization |
| title_auth | The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization |
| title_exact_search | The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization |
| title_full | The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization vorgelegt 2018 von Peipei Zhang |
| title_fullStr | The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization vorgelegt 2018 von Peipei Zhang |
| title_full_unstemmed | The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization vorgelegt 2018 von Peipei Zhang |
| title_short | The Role of the Interferon Regulatory Factor 8 in Retinal Microglia Cells during Retinal Homeostasis and Formation of Choroidal Neovascularization |
| title_sort | the role of the interferon regulatory factor 8 in retinal microglia cells during retinal homeostasis and formation of choroidal neovascularization |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1204266344/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031836636&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT zhangpeipei theroleoftheinterferonregulatoryfactor8inretinalmicrogliacellsduringretinalhomeostasisandformationofchoroidalneovascularization |
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