Superresolution optical microscopy: the quest for enhanced resolution and contrast
This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provid...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cham
Springer
[2020]
|
| Schriftenreihe: | Springer Series in Optical Sciences
Volume 227 |
| Schlagworte: | |
| Zusammenfassung: | This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike |
| Beschreibung: | XXII, 403 Seiten Illustrationen, Diagramme |
| ISBN: | 9783030216900 |
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| 520 | |a Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. | ||
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Datensatz im Suchindex
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| author | Masters, Barry R. |
| author_facet | Masters, Barry R. |
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| building | Verbundindex |
| bvnumber | BV046324497 |
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| discipline | Physik |
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| id | DE-604.BV046324497 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:41:40Z |
| institution | BVB |
| isbn | 9783030216900 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031701453 |
| oclc_num | 1155067480 |
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| physical | XXII, 403 Seiten Illustrationen, Diagramme |
| publishDate | 2020 |
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| publisher | Springer |
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| series | Springer Series in Optical Sciences |
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| spelling | Masters, Barry R. Verfasser aut Superresolution optical microscopy the quest for enhanced resolution and contrast Cham Springer [2020] © 2020 XXII, 403 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Springer Series in Optical Sciences Volume 227 This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike bicssc bisacsh Spectroscopy Microscopy Microwaves Optical engineering Lasers Photonics History Physics Fernfeld (DE-588)4154023-2 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf Hochauflösendes Verfahren (DE-588)4287503-1 gnd rswk-swf Hardcover, Softcover / Physik, Astronomie/Mechanik, Akustik Mikroskopie (DE-588)4039238-7 s Fernfeld (DE-588)4154023-2 s Hochauflösendes Verfahren (DE-588)4287503-1 s DE-604 Erscheint auch als Online-Ausgabe 978-3-030-21691-7 Springer Series in Optical Sciences Volume 227 (DE-604)BV000000237 227 |
| spellingShingle | Masters, Barry R. Superresolution optical microscopy the quest for enhanced resolution and contrast Springer Series in Optical Sciences bicssc bisacsh Spectroscopy Microscopy Microwaves Optical engineering Lasers Photonics History Physics Fernfeld (DE-588)4154023-2 gnd Mikroskopie (DE-588)4039238-7 gnd Hochauflösendes Verfahren (DE-588)4287503-1 gnd |
| subject_GND | (DE-588)4154023-2 (DE-588)4039238-7 (DE-588)4287503-1 |
| title | Superresolution optical microscopy the quest for enhanced resolution and contrast |
| title_auth | Superresolution optical microscopy the quest for enhanced resolution and contrast |
| title_exact_search | Superresolution optical microscopy the quest for enhanced resolution and contrast |
| title_full | Superresolution optical microscopy the quest for enhanced resolution and contrast |
| title_fullStr | Superresolution optical microscopy the quest for enhanced resolution and contrast |
| title_full_unstemmed | Superresolution optical microscopy the quest for enhanced resolution and contrast |
| title_short | Superresolution optical microscopy |
| title_sort | superresolution optical microscopy the quest for enhanced resolution and contrast |
| title_sub | the quest for enhanced resolution and contrast |
| topic | bicssc bisacsh Spectroscopy Microscopy Microwaves Optical engineering Lasers Photonics History Physics Fernfeld (DE-588)4154023-2 gnd Mikroskopie (DE-588)4039238-7 gnd Hochauflösendes Verfahren (DE-588)4287503-1 gnd |
| topic_facet | bicssc bisacsh Spectroscopy Microscopy Microwaves Optical engineering Lasers Photonics History Physics Fernfeld Mikroskopie Hochauflösendes Verfahren |
| volume_link | (DE-604)BV000000237 |
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