Investigating the metabolism of Pichia pastoris for improved recombinant protein production:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
Apprimus Verlag
2019
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Applied Microbiology
Volume 15 |
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | XIII, 172 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 290 g |
| ISBN: | 9783863597832 3863597834 |
| DOI: | 10.18154/RWTH-2019-10507 |
Internformat
MARC
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| 100 | 1 | |a Förster, Jan |d 1985- |e Verfasser |0 (DE-588)1199888842 |4 aut | |
| 245 | 1 | 0 | |a Investigating the metabolism of Pichia pastoris for improved recombinant protein production |c Jan Förster |
| 250 | |a 1. Auflage | ||
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| 502 | |b Dissertation |c RWTH Aachen University |d 2019 | ||
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENT
CONTENT
SUMMARY
...........................................................................................................................
ILL
ZUSAMMENFASSUNG
...........................................................................................................
V
LIST
OF
ABBREVIATIONS
.......................................................................................................
VII
LIST
OF
FIGURES
...................................................................................................................
XI
LIST
OF
TABLES
..................................................................................................................
XIII
1.
GENERAL
INTRODUCTION
................................................................................................
1
1.1.
RECOMBINANT
PROTEIN
PRODUCTION:
AN
OVERVIEW
.................................................
1
PROKARYOTIC
SYSTEMS
.................................................................................................
2
LOWER
EUKARYOTIC
SYSTEMS
.......................................................................................
2
HIGHER
EUKARYOTIC
SYSTEMS
.......................................................................................
3
1.2.
PICHIA
PASTORIS:
A
HOST
ORGANISM
EXTRAORDINAIRE
................................................
5
1.3.
STATE-OF-THE-ART:
TECHNIQUES
AND
BOTTLENECKS
.................................................
8
1.4.
THE
CELL
METABOLISM:
A
NOVEL
TARGET
FOR
OPTIMIZATION
.........................................
9
1.5.
SCOPE
OF
THE
THESIS
............................................................................................
12
2.
RESULTS
.....................................................................................................................
19
2.1.
A
BLUEPRINT
OF
THE
AMINO
ACID
BIOSYNTHESIS
NETWORK
OF
HEMIASCOMYCETES
....
19
2.1.1.
INTRODUCTION
...............................................................................................
19
2.1.2.
MATERIALS
AND
METHODS
.............................................................................
21
2.1.3.
RESULTS
......................................................................................................
24
2.1.4.
DISCUSSION
.................................................................................................
36
2.2.
DEREGULATION
OF
THE
LEUCINE
BIOSYNTHESIS
PATHWAY
IN
PICHIA
PASTORIS
............ 43
2.2.1.
INTRODUCTION
...............................................................................................
43
2.2.2.
METHODS
....................................................................................................49
2.2.3.
RESULTS
......................................................................................................
53
2.2.4.
DISCUSSION
.................................................................................................67
2.3.
MAGNETIZATION
OF
PICHIA
PASTORIS
.......................................................................
75
2.3.1.
INTRODUCTION
...............................................................................................
75
2.3.2.
MATERIALS
AND
METHODS
.............................................................................
78
2.3.3.
RESULTS
......................................................................................................
61
2.3.4.
DISCUSSION
.................................................................................................
87
2.4.
GLYCOSYLATION
OF
RECOMBINANT
E.
COLI
APPA
PHYTASE
........................................
91
2.4.1.
INTRODUCTION
...............................................................................................
91
CONTENT
2.4.2.
MATERIALS
&
METHODS
..................................................................................
96
2.4.3.
RESULTS
.......................................................................................................
98
2.4.4.
DISCUSSION
................................................................................................
104
3.
GENERAL
DISCUSSION
AND
OUTLOOK
.........................................................................
111
3.1.
GENERAL
DISCUSSION
..........................................................................................
111
3.2.
OUTLOOK
..............................................................................................................
119
4.
REFERENCES
.............................................................................................................
123
5.
APPENDIX
................................................................................................................
141
6.
CURRICULUM
VITAE
....................................................................................................
171
II
|
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| author | Förster, Jan 1985- |
| author_GND | (DE-588)1199888842 |
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| discipline | Allgemeine Naturwissenschaft |
| doi_str_mv | 10.18154/RWTH-2019-10507 |
| edition | 1. Auflage |
| format | Thesis Book |
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| id | DE-604.BV046299179 |
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| indexdate | 2024-07-10T08:40:59Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783863597832 3863597834 |
| language | English |
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| publishDateSearch | 2019 |
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| publisher | Apprimus Verlag |
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| series | Applied Microbiology |
| series2 | Applied Microbiology |
| spelling | Förster, Jan 1985- Verfasser (DE-588)1199888842 aut Investigating the metabolism of Pichia pastoris for improved recombinant protein production Jan Förster 1. Auflage Aachen Apprimus Verlag 2019 XIII, 172 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 290 g txt rdacontent n rdamedia nc rdacarrier Applied Microbiology Volume 15 Dissertation RWTH Aachen University 2019 Herstellung (DE-588)4159653-5 gnd rswk-swf Pichia pastoris (DE-588)4407211-9 gnd rswk-swf Rekombinantes Protein (DE-588)4277353-2 gnd rswk-swf Biosynthese (DE-588)4006902-3 gnd rswk-swf Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd rswk-swf Leucin (DE-588)4167477-7 gnd rswk-swf Stoffwechsel (DE-588)4057699-1 gnd rswk-swf Naturwissenschaften metabolism Pichia pastoris protein (DE-588)4113937-9 Hochschulschrift gnd-content Pichia pastoris (DE-588)4407211-9 s Stoffwechsel (DE-588)4057699-1 s Rekombinantes Protein (DE-588)4277353-2 s Leucin (DE-588)4167477-7 s Biosynthese (DE-588)4006902-3 s DE-604 Gentechnisch veränderter Organismus (DE-588)4353579-3 s Herstellung (DE-588)4159653-5 s Apprimus Verlag (DE-588)1068101474 pbl Erscheint auch als Online-Ausgabe, PDF https://doi.org/10.18154/RWTH-2019-10507 Applied Microbiology Volume 15 (DE-604)BV042644516 15 https://doi.org/10.18154/RWTH-2019-10507 Resolving-System kostenfrei Volltext X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=994962f2cc964d7fb966952d2280b8a1&prov=M&dok_var=1&dok_ext=htm Resolving-System kostenfrei Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031676539&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Förster, Jan 1985- Investigating the metabolism of Pichia pastoris for improved recombinant protein production Applied Microbiology Herstellung (DE-588)4159653-5 gnd Pichia pastoris (DE-588)4407211-9 gnd Rekombinantes Protein (DE-588)4277353-2 gnd Biosynthese (DE-588)4006902-3 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Leucin (DE-588)4167477-7 gnd Stoffwechsel (DE-588)4057699-1 gnd |
| subject_GND | (DE-588)4159653-5 (DE-588)4407211-9 (DE-588)4277353-2 (DE-588)4006902-3 (DE-588)4353579-3 (DE-588)4167477-7 (DE-588)4057699-1 (DE-588)4113937-9 |
| title | Investigating the metabolism of Pichia pastoris for improved recombinant protein production |
| title_auth | Investigating the metabolism of Pichia pastoris for improved recombinant protein production |
| title_exact_search | Investigating the metabolism of Pichia pastoris for improved recombinant protein production |
| title_full | Investigating the metabolism of Pichia pastoris for improved recombinant protein production Jan Förster |
| title_fullStr | Investigating the metabolism of Pichia pastoris for improved recombinant protein production Jan Förster |
| title_full_unstemmed | Investigating the metabolism of Pichia pastoris for improved recombinant protein production Jan Förster |
| title_short | Investigating the metabolism of Pichia pastoris for improved recombinant protein production |
| title_sort | investigating the metabolism of pichia pastoris for improved recombinant protein production |
| topic | Herstellung (DE-588)4159653-5 gnd Pichia pastoris (DE-588)4407211-9 gnd Rekombinantes Protein (DE-588)4277353-2 gnd Biosynthese (DE-588)4006902-3 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Leucin (DE-588)4167477-7 gnd Stoffwechsel (DE-588)4057699-1 gnd |
| topic_facet | Herstellung Pichia pastoris Rekombinantes Protein Biosynthese Gentechnisch veränderter Organismus Leucin Stoffwechsel Hochschulschrift |
| url | https://doi.org/10.18154/RWTH-2019-10507 http://deposit.dnb.de/cgi-bin/dokserv?id=994962f2cc964d7fb966952d2280b8a1&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031676539&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV042644516 |
| work_keys_str_mv | AT forsterjan investigatingthemetabolismofpichiapastorisforimprovedrecombinantproteinproduction AT apprimusverlag investigatingthemetabolismofpichiapastorisforimprovedrecombinantproteinproduction |