The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Bonn
2018
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| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 107 Seiten Illustrationen 21 cm |
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| 245 | 1 | 0 | |a The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy |c vorgelegt von Ermanila Dhana |
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Datensatz im Suchindex
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|---|---|
| adam_text | TABLE OF CONTENTS
1
SUMMARY.................................................................................................................3
2 TABLE OF
CONTENTS..................................................................................................
4
3
INTRODUCTION...........................................................................................................7
3.1 THE IMMUNE SYSTEM
.........................................................................................................
7
3.2 REGULATORY T
CELLS.............................................................................................................
7
3.2.1 REGULATORY T CELL
DEVELOPMENT.................................................................................7
3.2.2 MECHANISMS OF T REGULATORY CELL
SUPPRESSION.........................................................9
3.2.3 IL-10 SIGNALING
PATHWAY..........................................................................................11
3.2.4 PD-1 SIGNALING
PATHWAY...........................................................................................12
3.3 B
CELLS...............................................................................................................................
13
3.3.1 MECHANISMS OF AUTOREACTIVE B CELL SUPPRESSION
...................................................
13
3.4 SUPPRESSION OF B CELLS BY FOXP3+
TREGS........................................................................16
3.5 REGULATION OF DCS BY FOXP3+
TREGS...............................................................................
16
3.6 THE
KIDNEY.......................................................................................................................17
3.6.1 CHRONIC KIDNEY DISEASES AND FIBROSIS
....................................................................
18
3.6.2 MOUSE MODELS TO STUDY CRYSTAL-INDUCED KIDNEY FIBROSIS
......................................
19
3.6.3 NLRP3
INFLAMMASOME............................................................................................
21
4 AIMS OF THE
STUDY................................................................................................
22
5 MATERIALS AND METHODS
......................................................................................
23
5.1
MATERIALS..........................................................................................................................23
5.1.1 EQUIPMENT, CONSUMABLES, CHEMICALS AND
REAGENTS.............................................23
5.1.2 BUFFERS AND
MEDIA....................................................................................................26
5.1.3
ANTIBODIES................................................................................................................
28
5.1.4 PRIMARY
ANTIBODIES.................................................................................................
29
5.1.5
SOFTWARES..................................................................................................................30
5.1.6
KITS.............................................................................................................................30
5.1.7 MOUSE
STRAINS...........................................................................................................
31
5.2
METHODS...........................................................................................................................32
5.2.1 TREATMENT OF
MICE...................................................................................................
32
5.2.2 CELL
PREPARATION......................................................................................................
32
5.2.3 NUCLEIC ACID
BIOCHEMISTRY.....................................................................................
36
5.2.4 FLOW
CYTOMETRY......................................................................................................
37
5.2.5 PROTEIN
BIOCHEMISTRY..............................................................................................
39
6
RESULTS................................................................................................................
43
6.1 1- IDENTIFICATION OF KEY MOLECULES OF THE PD-L/PD-LL SIGNALING
PATHWAY THAT INDUCE
B CELL APOPTOSIS MEDIATED BY ANTIGEN- SPECIFIC T REGULATORY
CELLS.......................................43
6.1.1 ANTIGEN-SPECIFIC TREGS INDUCE APOPTOSIS IN AUTOREACTIVE B CELLS BY
UPREGULATING
PD-1 EXPRESSION ON THE B CELLS IN
VITRO..............................................................................43
6.1.2 ACTIVATED B CELLS INCREASE PD-1 EXPRESSION UPON BCR STIMULATION-
THE SIGNALING
PATHWAY INVOLVES CA^+
SIGNALING........................................................................................
45
6.1.3 FURTHER PD-1 UPREGULATION ON B CELLS DUE TO INTERACTION WITH
ANTIGEN-SPECIFIC
TREGS IS MEDIATED THROUGH SOLUBLE
FACTORS........................................................................
51
6.1.4 CONNEXIN 43 HEMICHANNELS PLAY AN IMPORTANT ROLE IN UPREGULATING
PD-1 ON B
CELLS 54
6.1.5 METABOLITES OF NAD+ INDUCE PD-1 UPREGULATION ON THE ACTIVATED B
CELLS
...........
57
6.2 II- ACTIVATED T REGULATORY CELLS REGULATE NLRP3 INFLAMMASOME
ACTIVATION IN RENAL
DENDRITIC
CELLS...........................................................................................................................
62
6.2.1 DIFFERENT HEMATOPOIETIC CELL POPULATIONS PRODUCE IL-10 IN THE
KIDNEY UNDER
INFLAMMATORY
CONDITIONS.....................................................................................................62
6.2.2 DEPLETION OF FOXP3+ TREGS ATTENUATES KIDNEY INFLAMMATION AND
FIBROSIS
..........
64
6.2.3 ROLE OF IL-10+ T REGULATORY CELLS IN KIDNEY INFLAMMATION
....................................
66
6.2.4 IL-10 DEFICIENT FOXP3+ CELLS CANNOT PROTECT THE KIDNEY FROM
INFLAMMATION AND
FIBROSIS..................................................................................................................................
67
6.2.5 STUDYING NLRP3 INFLAMMASOME ACTIVATION AND REGULATION IN RENAL DCS
IN VITRO
71
7
DISCUSSION...........................................................................................................
79
7.1 I- IDENTIFICATION OF KEY MOLECULES OF THE PD-L/PD-LL SIGNALING
PATHWAY THAT INDUCE
B CELL APOPTOSIS MEDIATED BY ANTIGEN SPECIFIC T REGULATORY
CELLS.........................................79
7.2 II- ACTIVATED T REGULATORY CELLS REGULATE NLRP3 INFLAMMASOME
ACTIVATION IN RENAL
DENDRITIC
CELLS...........................................................................................................................84
8
REFERENCES............................................................................................................89
9 ABBREVIATIONS
......
.
..............................................
105
10 ACKNOWLEDGMENTS................................ 107
|
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| author_GND | (DE-588)1189764385 |
| author_facet | Dhana, Ermanila |
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| indexdate | 2024-07-10T08:37:27Z |
| institution | BVB |
| language | English |
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| spelling | Dhana, Ermanila Verfasser (DE-588)1189764385 aut The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy vorgelegt von Ermanila Dhana Bonn 2018 107 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Rheinische Friedrich-Wilhelms-Universität Bonn 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1192918606/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031559691&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dhana, Ermanila The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy |
| subject_GND | (DE-588)4113937-9 |
| title | The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy |
| title_auth | The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy |
| title_exact_search | The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy |
| title_full | The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy vorgelegt von Ermanila Dhana |
| title_fullStr | The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy vorgelegt von Ermanila Dhana |
| title_full_unstemmed | The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy vorgelegt von Ermanila Dhana |
| title_short | The regulatory function of Tregs in autoimmunity and crystal-induced nephropathy |
| title_sort | the regulatory function of tregs in autoimmunity and crystal induced nephropathy |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1192918606/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031559691&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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