Antibody Engineering:
Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2001
|
| Schriftenreihe: | Springer Lab Manuals
|
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more. This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered. In particular, protocols on the following topics are provided: Hybridoma immortalisation Generation and screening of antibody gene libraries from human donors, mice and rabbits Antibody selection on immunotubes, cells, tissues; proximity and step-back selections Creation of human monoclonal antibodies to toxic or highly pathogenic agents without immunisation Improvment of antibody binding Antibody humanisation Genetic fusions for the production of multifunctional antibody derivatives Radiolabelled recombinant antibodies Bispecific antibodies Antibody - enzyme fusions Intracellular antibodies Determination of affinity and specificity Computer analysis of antibody sequence and structure Epitope analysis by various phage display systems and peptide spot membranes Eukaryotic (plant, baculovirus, yeast, mammalian cells) and prokaryotic production systems for recombinant antibodies Purification systems Xenograft mice Emerging technologies |
| Beschreibung: | 1 Online-Ressource (XII, 790 p. 125 illus., 9 illus. in color) |
| ISBN: | 9783662046050 |
| DOI: | 10.1007/978-3-662-04605-0 |
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Datensatz im Suchindex
| _version_ | 1804180489602334720 |
|---|---|
| any_adam_object | |
| author2 | Kontermann, Roland Dübel, Stefan |
| author2_role | edt edt |
| author2_variant | r k rk s d sd |
| author_facet | Kontermann, Roland Dübel, Stefan |
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| collection | ZDB-2-SBL |
| ctrlnum | (ZDB-2-SBL)978-3-662-04605-0 (OCoLC)1119105746 (DE-599)BVBBV046148837 |
| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Medizin |
| doi_str_mv | 10.1007/978-3-662-04605-0 |
| format | Electronic eBook |
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| genre_facet | Aufsatzsammlung |
| id | DE-604.BV046148837 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:36:35Z |
| institution | BVB |
| isbn | 9783662046050 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031529014 |
| oclc_num | 1119105746 |
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| physical | 1 Online-Ressource (XII, 790 p. 125 illus., 9 illus. in color) |
| psigel | ZDB-2-SBL ZDB-2-SBL_2000/2004 ZDB-2-SBL ZDB-2-SBL_2000/2004 |
| publishDate | 2001 |
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| publisher | Springer Berlin Heidelberg |
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| spelling | Antibody Engineering edited by Roland Kontermann, Stefan Dübel Berlin, Heidelberg Springer Berlin Heidelberg 2001 1 Online-Ressource (XII, 790 p. 125 illus., 9 illus. in color) txt rdacontent c rdamedia cr rdacarrier Springer Lab Manuals Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more. This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered. In particular, protocols on the following topics are provided: Hybridoma immortalisation Generation and screening of antibody gene libraries from human donors, mice and rabbits Antibody selection on immunotubes, cells, tissues; proximity and step-back selections Creation of human monoclonal antibodies to toxic or highly pathogenic agents without immunisation Improvment of antibody binding Antibody humanisation Genetic fusions for the production of multifunctional antibody derivatives Radiolabelled recombinant antibodies Bispecific antibodies Antibody - enzyme fusions Intracellular antibodies Determination of affinity and specificity Computer analysis of antibody sequence and structure Epitope analysis by various phage display systems and peptide spot membranes Eukaryotic (plant, baculovirus, yeast, mammalian cells) and prokaryotic production systems for recombinant antibodies Purification systems Xenograft mice Emerging technologies Cell Biology Immunology Biotechnology Biochemistry, general Cytology Biochemistry Herstellung (DE-588)4159653-5 gnd rswk-swf Labormedizin (DE-588)4073931-4 gnd rswk-swf Antikörper (DE-588)4002290-0 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Rekombinantes Protein (DE-588)4277353-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Antikörper (DE-588)4002290-0 s Rekombinantes Protein (DE-588)4277353-2 s Herstellung (DE-588)4159653-5 s Labormedizin (DE-588)4073931-4 s DE-604 Labortechnik (DE-588)4123602-6 s Kontermann, Roland edt Dübel, Stefan edt Erscheint auch als Druck-Ausgabe 9783540413547 Erscheint auch als Druck-Ausgabe 9783662046067 https://doi.org/10.1007/978-3-662-04605-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Antibody Engineering Cell Biology Immunology Biotechnology Biochemistry, general Cytology Biochemistry Herstellung (DE-588)4159653-5 gnd Labormedizin (DE-588)4073931-4 gnd Antikörper (DE-588)4002290-0 gnd Labortechnik (DE-588)4123602-6 gnd Rekombinantes Protein (DE-588)4277353-2 gnd |
| subject_GND | (DE-588)4159653-5 (DE-588)4073931-4 (DE-588)4002290-0 (DE-588)4123602-6 (DE-588)4277353-2 (DE-588)4143413-4 |
| title | Antibody Engineering |
| title_auth | Antibody Engineering |
| title_exact_search | Antibody Engineering |
| title_full | Antibody Engineering edited by Roland Kontermann, Stefan Dübel |
| title_fullStr | Antibody Engineering edited by Roland Kontermann, Stefan Dübel |
| title_full_unstemmed | Antibody Engineering edited by Roland Kontermann, Stefan Dübel |
| title_short | Antibody Engineering |
| title_sort | antibody engineering |
| topic | Cell Biology Immunology Biotechnology Biochemistry, general Cytology Biochemistry Herstellung (DE-588)4159653-5 gnd Labormedizin (DE-588)4073931-4 gnd Antikörper (DE-588)4002290-0 gnd Labortechnik (DE-588)4123602-6 gnd Rekombinantes Protein (DE-588)4277353-2 gnd |
| topic_facet | Cell Biology Immunology Biotechnology Biochemistry, general Cytology Biochemistry Herstellung Labormedizin Antikörper Labortechnik Rekombinantes Protein Aufsatzsammlung |
| url | https://doi.org/10.1007/978-3-662-04605-0 |
| work_keys_str_mv | AT kontermannroland antibodyengineering AT dubelstefan antibodyengineering |