Resin Microscopy and On-Section Immunocytochemistry:
Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. Resin-embedded tissue is routinely used and new techniques...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2001
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Springer Lab Manuals
|
| Schlagworte: | |
| Online-Zugang: | UBR01 URL des Erstveröffentlichers |
| Zusammenfassung: | Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired and appropriate choices made. In the first part of the book, the background of the various resins available are discussed and information on inexpensive alternative technologies where they exist is provided. The various steps involved in tissue processing, beginning with fixation, are first described in theory, then detailed protocols are presented for their application, including troubleshooting sections. The second part rationalises the great variety of labelling methods that are commonly used for "on-section" cytochemistry and immunocytochemistry, where colloidal gold is currently overwhelmingly the most popular marker. The principles behind the usage of various markers and their limitations and advantages are discussed |
| Beschreibung: | 1 Online-Ressource (XIX, 273 p) |
| ISBN: | 9783642569302 |
| DOI: | 10.1007/978-3-642-56930-2 |
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Datensatz im Suchindex
| _version_ | 1804180489040297984 |
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| any_adam_object | |
| author | Newman, Geoffrey R. Hobot, Jan A. |
| author_facet | Newman, Geoffrey R. Hobot, Jan A. |
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| author_sort | Newman, Geoffrey R. |
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| ctrlnum | (ZDB-2-SBL)978-3-642-56930-2 (OCoLC)725313930 (DE-599)BVBBV046148594 |
| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-3-642-56930-2 |
| edition | Second Edition |
| format | Electronic eBook |
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| id | DE-604.BV046148594 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:36:34Z |
| institution | BVB |
| isbn | 9783642569302 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031528771 |
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| physical | 1 Online-Ressource (XIX, 273 p) |
| psigel | ZDB-2-SBL ZDB-2-SBL_2000/2004 ZDB-2-SBL ZDB-2-SBL_2000/2004 |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Springer Berlin Heidelberg |
| record_format | marc |
| series2 | Springer Lab Manuals |
| spelling | Newman, Geoffrey R. Verfasser aut Resin Microscopy and On-Section Immunocytochemistry by Geoffrey R. Newman, Jan A. Hobot Second Edition Berlin, Heidelberg Springer Berlin Heidelberg 2001 1 Online-Ressource (XIX, 273 p) txt rdacontent c rdamedia cr rdacarrier Springer Lab Manuals Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired and appropriate choices made. In the first part of the book, the background of the various resins available are discussed and information on inexpensive alternative technologies where they exist is provided. The various steps involved in tissue processing, beginning with fixation, are first described in theory, then detailed protocols are presented for their application, including troubleshooting sections. The second part rationalises the great variety of labelling methods that are commonly used for "on-section" cytochemistry and immunocytochemistry, where colloidal gold is currently overwhelmingly the most popular marker. The principles behind the usage of various markers and their limitations and advantages are discussed Cell Biology Immunology Biochemistry, general Cytology Biochemistry Elektronenmikroskopie (DE-588)4014327-2 gnd rswk-swf Markierung Chemie (DE-588)4201779-8 gnd rswk-swf Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Gewebe (DE-588)4020840-0 gnd rswk-swf Schnittpräparat (DE-588)4202716-0 gnd rswk-swf Kunstharz (DE-588)4033651-7 gnd rswk-swf Einbettung Mikroskopie (DE-588)4321430-7 gnd rswk-swf Gewebe (DE-588)4020840-0 s Kunstharz (DE-588)4033651-7 s Einbettung Mikroskopie (DE-588)4321430-7 s DE-604 Schnittpräparat (DE-588)4202716-0 s Markierung Chemie (DE-588)4201779-8 s Elektronenmikroskopie (DE-588)4014327-2 s Immuncytochemie (DE-588)4161368-5 s 1\p DE-604 Hobot, Jan A. aut Erscheint auch als Druck-Ausgabe 9783642477317 Erscheint auch als Druck-Ausgabe 9783540672777 Erscheint auch als Druck-Ausgabe 9783642569319 https://doi.org/10.1007/978-3-642-56930-2 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Newman, Geoffrey R. Hobot, Jan A. Resin Microscopy and On-Section Immunocytochemistry Cell Biology Immunology Biochemistry, general Cytology Biochemistry Elektronenmikroskopie (DE-588)4014327-2 gnd Markierung Chemie (DE-588)4201779-8 gnd Immuncytochemie (DE-588)4161368-5 gnd Gewebe (DE-588)4020840-0 gnd Schnittpräparat (DE-588)4202716-0 gnd Kunstharz (DE-588)4033651-7 gnd Einbettung Mikroskopie (DE-588)4321430-7 gnd |
| subject_GND | (DE-588)4014327-2 (DE-588)4201779-8 (DE-588)4161368-5 (DE-588)4020840-0 (DE-588)4202716-0 (DE-588)4033651-7 (DE-588)4321430-7 |
| title | Resin Microscopy and On-Section Immunocytochemistry |
| title_auth | Resin Microscopy and On-Section Immunocytochemistry |
| title_exact_search | Resin Microscopy and On-Section Immunocytochemistry |
| title_full | Resin Microscopy and On-Section Immunocytochemistry by Geoffrey R. Newman, Jan A. Hobot |
| title_fullStr | Resin Microscopy and On-Section Immunocytochemistry by Geoffrey R. Newman, Jan A. Hobot |
| title_full_unstemmed | Resin Microscopy and On-Section Immunocytochemistry by Geoffrey R. Newman, Jan A. Hobot |
| title_short | Resin Microscopy and On-Section Immunocytochemistry |
| title_sort | resin microscopy and on section immunocytochemistry |
| topic | Cell Biology Immunology Biochemistry, general Cytology Biochemistry Elektronenmikroskopie (DE-588)4014327-2 gnd Markierung Chemie (DE-588)4201779-8 gnd Immuncytochemie (DE-588)4161368-5 gnd Gewebe (DE-588)4020840-0 gnd Schnittpräparat (DE-588)4202716-0 gnd Kunstharz (DE-588)4033651-7 gnd Einbettung Mikroskopie (DE-588)4321430-7 gnd |
| topic_facet | Cell Biology Immunology Biochemistry, general Cytology Biochemistry Elektronenmikroskopie Markierung Chemie Immuncytochemie Gewebe Schnittpräparat Kunstharz Einbettung Mikroskopie |
| url | https://doi.org/10.1007/978-3-642-56930-2 |
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