Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology
Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products wi...
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| Weitere Verfasser: | , , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2002
|
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier! |
| Beschreibung: | 1 Online-Ressource (VIII, 205 p. 539 illus., 528 illus. in color) |
| ISBN: | 9783642593970 |
| DOI: | 10.1007/978-3-642-59397-0 |
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| author2 | Dietmaier, Wolfgang Wittwer, Carl Sivasubramanian, Natarajan |
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| discipline | Biologie Medizin |
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| isbn | 9783642593970 |
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| spelling | Rapid Cycle Real-Time PCR — Methods and Applications Genetics and Oncology edited by Wolfgang Dietmaier, Carl Wittwer, Natarajan Sivasubramanian Berlin, Heidelberg Springer Berlin Heidelberg 2002 1 Online-Ressource (VIII, 205 p. 539 illus., 528 illus. in color) txt rdacontent c rdamedia cr rdacarrier Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier! Oncology Molecular Medicine Oncology Medicine Onkologie (DE-588)4075658-0 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Polymerase-Kettenreaktion (DE-588)4256726-9 s Onkologie (DE-588)4075658-0 s DE-604 Molekulargenetik (DE-588)4039987-4 s Dietmaier, Wolfgang edt Wittwer, Carl edt Sivasubramanian, Natarajan edt Erscheint auch als Druck-Ausgabe 9783642639654 Erscheint auch als Druck-Ausgabe 9783540426004 Erscheint auch als Druck-Ausgabe 9783642593987 https://doi.org/10.1007/978-3-642-59397-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Rapid Cycle Real-Time PCR — Methods and Applications Genetics and Oncology Oncology Molecular Medicine Oncology Medicine Onkologie (DE-588)4075658-0 gnd Molekulargenetik (DE-588)4039987-4 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4075658-0 (DE-588)4039987-4 (DE-588)4256726-9 (DE-588)4143413-4 |
| title | Rapid Cycle Real-Time PCR — Methods and Applications Genetics and Oncology |
| title_auth | Rapid Cycle Real-Time PCR — Methods and Applications Genetics and Oncology |
| title_exact_search | Rapid Cycle Real-Time PCR — Methods and Applications Genetics and Oncology |
| title_full | Rapid Cycle Real-Time PCR — Methods and Applications Genetics and Oncology edited by Wolfgang Dietmaier, Carl Wittwer, Natarajan Sivasubramanian |
| title_fullStr | Rapid Cycle Real-Time PCR — Methods and Applications Genetics and Oncology edited by Wolfgang Dietmaier, Carl Wittwer, Natarajan Sivasubramanian |
| title_full_unstemmed | Rapid Cycle Real-Time PCR — Methods and Applications Genetics and Oncology edited by Wolfgang Dietmaier, Carl Wittwer, Natarajan Sivasubramanian |
| title_short | Rapid Cycle Real-Time PCR — Methods and Applications |
| title_sort | rapid cycle real time pcr methods and applications genetics and oncology |
| title_sub | Genetics and Oncology |
| topic | Oncology Molecular Medicine Oncology Medicine Onkologie (DE-588)4075658-0 gnd Molekulargenetik (DE-588)4039987-4 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Oncology Molecular Medicine Oncology Medicine Onkologie Molekulargenetik Polymerase-Kettenreaktion Aufsatzsammlung |
| url | https://doi.org/10.1007/978-3-642-59397-0 |
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