Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen, Lahn
VVB Laufersweiler Verlag
2019
|
| Schriftenreihe: | Edition Scientifique
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 165 Seiten Illustrationen, Diagramme |
| ISBN: | 9783835967564 3835967568 |
Internformat
MARC
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| 020 | |a 9783835967564 |c : EUR 34.80 (DE), EUR 35.80 (AT) |9 978-3-8359-6756-4 | ||
| 020 | |a 3835967568 |9 3-8359-6756-8 | ||
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| 044 | |a gw |c XA-DE-HE | ||
| 049 | |a DE-188 | ||
| 084 | |a 630 |2 sdnb | ||
| 100 | 1 | |a Mahmoud Fawzy Mohammed, Ahmad |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany |c submitted by Ahmad Mahmoud Fawzy Mohammed |
| 264 | 1 | |a Gießen, Lahn |b VVB Laufersweiler Verlag |c 2019 | |
| 300 | |a XIII, 165 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Edition Scientifique | |
| 502 | |b Dissertation |c Justus-Liebig-Universität Gießen |d 2019 | ||
| 650 | 7 | |a cattle |2 cabt | |
| 650 | 7 | |a Mycobacterium avium subsp. paratuberculosis |2 cabt | |
| 650 | 7 | |a epidemiology |2 cabt | |
| 650 | 7 | |a disease prevalence |2 cabt | |
| 650 | 7 | |a polymerase chain reaction |2 cabt | |
| 650 | 7 | |a germany |2 cabt | |
| 650 | 0 | 7 | |a Genotypisierung |0 (DE-588)7641829-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mycobacterium paratuberculosis |0 (DE-588)4520169-9 |2 gnd |9 rswk-swf |
| 653 | |a Doktorarbeit | ||
| 653 | |a Uni | ||
| 653 | |a Wissenschaft | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Mycobacterium paratuberculosis |0 (DE-588)4520169-9 |D s |
| 689 | 0 | 1 | |a Genotypisierung |0 (DE-588)7641829-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 710 | 2 | |a VVB LAUFERSWEILER Verlag |0 (DE-588)1065553803 |4 pbl | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031495490&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-031495490 | ||
Datensatz im Suchindex
| _version_ | 1804180432516808704 |
|---|---|
| adam_text | CONTENTS
I
CONTENTS
CONTENTS
...............................................................................................................................................
I
LIST
OF
ABBREVIATIONS
.......................................................................................................................
VI
LIST
OF
TABLES
.....................................................................................................................................
X
LIST
OF
FIGURES
.................................................................................................................................XII
1.
INTRODUCTION
........................................................................................................................
-
I
-
2.
REVIEW
OF
THE
LITERATURE
.....................................................................................................
-
3
-
2.1.
MYCOBACTERIA
..............................................................................................................
-
3
-
2.2.
MYCOBACTERIUM
AVIUM-INTRACELLULARE
COMPLEX
.......................................................
-
4
-
2.2.1.
MYCOBACTERIUM
AVIUM
SUBSP.
AVIUM
..................................................................
-
5
-
2.2.2.
MYCOBACTERIUM
AVIUM
SUBSP.
SILVATICUM
..........................................................
-
5
-
2.3.
MYCOBACTERIUM
AVIUM
SUBSP.
PARATUBERCULOSIS
......................................................
-
5
-
2.3.1.
ANIMAL
HOST
RANGE
...............................................................................................
-
5
-
2.3.2.
CLINICAL
FINDINGS
(JOHNE
*
S
DISEASE)
.....................................................................
-
6
-
2.3.3.
PATHOPHYSIOLOGY
..................................................................................................
-7-
2.3.4.
SEROLOGICAL
DIAGNOSIS
(SERUM
AND
MILK
ELISA)
................................................
-
8
-
2.3.5.
TRANSMISSION
........................................................................................................
-
8
-
2.3.6.
SURVIVAL
AND
RESISTANCE
........................................................................................
-
9
-
2.3.7.
CONTROL
................................................................................................................
-10-
2.3.8.
CULTURAL
AND
PHENOTYPIC
CHARACTERISTICS
..........................................................
-12
-
2.3.9.
MOLECULAR
CHARACTERISTICS
...................................................................................
-14
-
2.3.10.
ZOONOTIC
POTENTIAL
..........................................................................................
-15
-
2.4.
MAP
GENETIC
DIVERSITY
AND
ITS
ASSOCIATED
EFFECTS
........................ ........................
-15
-
2.4.1.
MAP
TYPES
...........................................................................................................
-15-
2.4.2.
GENOTYPING
METHODS
.........................................................................................
-16
-
2.4.2.1.
PULSED
FIELD
GEL
ELECTROPHORESIS
.................................................................
-16
-
2.4.2.2.
IS900-RESTRICTION
FRAGMENT
LENGTH
POLYMORPHISM
(IS900-RFLP)
.......
-17
-
2.4.2.3.
TANDEM
REPEATS-BASED
GENOTYPING
...........................................................
-
18
-
2.4.2.3.1.
MYCOBACTERIAL
INTERSPERSED
REPETITIVE
UNIT-VARIABLE
NUMBER
TR
.....
-18-
2.4.2.3.2.
MULTI-LOCUS
SHORT
SEQUENCE
REPEATS
(MLSSR)
...................................
-19
-
2.4.2.3.3.
REPEAT
COPY
NUMBER
DETERMINATION
BY
MELTING
CURVE
ANALYSIS
........
-
20
-
2.4.2.4.
COMBINATION
OF
DIFFERENT
GENOTYPING
MARKERS
........................................
-
22
-
2.4.2
5.
SINGLE
NUCLEOTIDE
POLYMORPHISMS
............................................................
-
24
-
CONTENTS
II
2.4.2.6.
WHOLE
GENOME
SEQUENCING
.......................................................................
-
25
-
2.4.3.
GENOTYPE-PHENOTYPE
ASSOCIATION
......................................................................
-
25
-
2.4.3.1.
STRAIN-DEPENDENT
GROWTH
PATTERNS
.............................................................
-
25
-
2.4.3.2.
STRAIN
DEPENDENT
EPIDEMIOLOGICAL
FEATURES
..............................................
-
26
-
2.4.3.3.
STRAIN-DEPENDENT
VIRULENCE
AND
INFECTIVITY
ATTRIBUTES
.............................
-
27
-
2.4.4.
WITHIN-HERD
AND
WITHIN-ANIMAL
STRAIN
DIVERSITY
.............................................
-
28
-
2.5.
PHENOTYPIC
TESTS
........................................................................................................
-
28
-
2.5.1.
MATRIX-ASSISTED
LASER
DESORPTION
IONISATION-TIME
OF
FLIGHT
MASS
SPECTROMETRY
-28-
2.5.2.
FOURIER
TRANSFORM
INFRARED
SPECTROSCOPY
.........................................................
-
29
-
3.
MATERIALS
AND
METHODS
......................................................................................................
-31-
3.1.
MYCOBACTERIAL
STRAINS,
ISOLATES
AND
CULTURES
..........................................................
-31-
3.2.
SUBCULTURING
MYCOBACTERIAL
STRAINS,
ISOLATES
AND
CULTURES
.....................................
-
33
-
3.3.
DNA
ISOLATION
.........................................................................................................
-
34
-
3.4.
MOLECULAR
CONFIRMATION
OF
THE
FIELD
ISOLATES/CULTURES
..........................................
-
34
-
3.4.1.
MAP
CULTURES
.......................................................................................................
-34-
3.4.2.
MAH
AND
MAA
ISOLATES
..................................................................................
-
34
-
3.5.
MAP
GENOTYPIC
CHARACTERISATION
AND
OPTIMISATION
OF
THE
GENOTYPING
METHODOLOGY
..-
35-
3.5.1.
TESTING
A
COST
EFFECTIVE
ALTERNATIVE
APPROACH
FOR
TR-BASED
MAP
GENOTYPING-
35
3.5.1.1.
MIRU-VNTR
PCR
...................................................................................
-36
-
3.5.1.2.
AGAROSE
GEL
ELECTROPHORESIS
......................................................................
-
38
-
3.5.1.3.
DETERMINATION
OF
MIRU-VNTR
ALLELES
...................................................
-
38
-
3.5.1.4.
MIRU-VNTR
NOMENCLATURE
.....................................................................
-
40
-
3.5.1.4.1.
INMV
PANEL
..........................................................................................
-40-
3.5.1.4.2.
MV
PANEL
..............................................................................................
-41-
3.5.1.5.
SSRPCR
....................................................................................................
-41
-
3.5.1.6.
DETERMINATION
OF
SSR
ALLELES
.....................................................................
-41-
3.5.1.7.
SSR
NOMENCLATURE
.....................................................................................
-
41
-
3.5.1.8.
CALCULATION
OF
ALLELIC
DIVERSITY
AND
DISCRIMINATORY
POWER
INDICES
.........
-
42
-
3.5.1.8.1.
MAP
CULTURES
USED
...............................................................................
-
42
-
3.5.1.8.2.
ALLELIC
DIVERSITY
OF
TR
LOCI
.................................................................
-
42
-
3.5.1.8.3.
DISCRIMINATORY
POWER
..........................................................................
-
42
-
CONTENTS
III
3.5.2.
SEARCHING
FOR
NOVEL
MAP
VNTR
LOCI
.............................................................
-
43
-
3.5.2.1.
IN
SILICO
SELECTION
OF
VNTR
CANDIDATES
AND
PRIMER
DESIGN
...................
-
43
-
3.5.2.2.
IN
VITRO
VERIFICATION
OF
VNTR
CANDIDATES
................................................
-
43
-
3.5.2.2.1.
MAP
CULTURES
USED
...............................................................................
-
43
-
3.5.2.2.2.
VNTR
CANDIDATES
*
PCR
.......................................................................
-
44
-
3.5.2.3.
DETERMINATION
OF
THE
ADDED
VALUE
OF
NOVEL
VNTR
LOCI
......................... -
44
-
3.5.2.3.1.
MAP
CULTURES
USED
.................................................................................
-44-
3.5.2.3.2.
DISCRIMINATORY
POWER
............................................................................
-
44
-
3.5.3.
INVESTIGATING
SEQUENCE
DATA
FOR
VNTR
7
LOCUS
................................................
-
44
-
3.5.3.1.
IN
SILICO
ANALYSIS
..........................................................................................
-
45
-
3.5.3.2.
IN
VITRO
ANALYSIS
......................................................................................
-45-
3.5.3.2.1.
PILOT
STUDY
..............................................................................................
-
45
-
3.5.3.2.2.
COMPREHENSIVE
ANALYSIS
.......................................................................
-
45
-
3.5.4.
DEVELOPMENT
OF
MCA
AS
A
MIRU-VNTR
ALLELE-DETERMINING
METHOD
..........
-
45
-
3.5.4.1.
MIRU-VNTR
MARKERS
...............................................................................
-
45
-
3.5.4.2.
CULTURES
USED
..............................................................................................
-
46
-
3.5.4.3.
PRIMER
DESIGN
..............................................................................................
-
47
-
3
5.4.4.
PCR
CONDITIONS
AND
MELTING
TEMPERATURE
CALCULATION
..............................
-
47
-
3.5.4.5.
CUTOFF-BASED
ALLELE
DETERMINATION
.............................................................
-
48
-
3.5.5.
HIERARCHICAL
TYPING
APPROACH
(SNP
+
INMV)
................................................
-
49
-
3.5.5.1.
INMV
PCR
..................................................................................................
-49-
3.5.5.2.
EVALUATION
OF
INMV
TYPES
AS
A
MEANS
FOR
DIFFERENTIATION
BETWEEN
MAP
TYPES
C
AND
S
................................................................................................................
-
49
-
3.5.5.2.1.
SNP-BASED
DIFFERENTIATION
BETWEEN
MAP
TYPES
C
AND
S
.................
-
49
-
3.5.5.2.2.
INMV-BASED
PHYLOGENETIC
ANALYSIS
....................................................
-
50
-
3.5.5.3.
INMV-GUIDED
SNP
ANALYSIS
FOR
MAP
TYPE
C
..........................................
-
50
-
3.5.5.4.
DISCRIMINATORY
POWER
EVALUATION
.............................................................
-
53
-
3.5.5.4.1.
MAP
CULTURES
USED
...............................................................................
-
53
-
3.5.5.4.2.
DISCRIMINATORY
POWER
CALCULATION
......................................................
-
53
-
3.5.5.5.
EVALUATION
OF
THE
PHYLOGENETIC
ACCURACY
OF
DIFFERENT
TR
PANEL
COMBINATIONS
-53-
3.6.
MAP
PHENOTYPIC
CHARACTERISATION
USING
FT-IR
....................................................
-
53
-
3.6.1.
MAP
CULTURES
AND
SAMPLE
PREPARATION
............................................................
-
53
-
3.6.2.
FT-IR
SPECTROSCOPIC
MEASUREMENT
...................................................................
-
54
-
CONTENTS
IV
3.6.3.
CLASSIFICATION
OF
MAP
FT-IR
SPECTRA
BASED
ON
ARTIFICIAL
NEURAL
NETWORKS
...
-
54
-
3.7.
STATISTICAL
ANALYSIS
...................................................................................................
-
55
-
4.
RESULTS
...................................................................................
-56-
4.1.
SUBCULTURING
MYCOBACTERIAL
STRAINS,
ISOLATES
AND
CULTURES
.....................................
-
56
-
4.2.
OPTIMISATION
OF
THE
MAP
GENOTYPING
METHODOLOGY
............................................
-
56
-
4.2.1.
TESTING
A
COST
EFFECTIVE
ALTERNATIVE
APPROACH
FOR
TR-BASED
MAP
GENOTYPING-
56
4.2.1.1.
ALLELIC
DIVERSITY
OF
TR
LOCI
........................................................................
-
68
-
4.2.1.2.
DISCRIMINATORY
POWER
OF
TYPING
PANELS
BOTH
INDEPENDENTLY
AND
COMBINED
..-
69-
4.2.2.
NOVEL
VNTRLOCI
...............................................................................................
-71
-
4.2.2.1.
SELECTED
VNTR
CANDIDATES
........................................................................
-71-
4.2.2.2.
IN
VITRO
VERIFICATION
OF
VNTR
CANDIDATES
................................................
-
74
-
4.2.2.3.
ADDED
VALUE
OF
VNTR
1798
LOCUS
...........................................................
-
75
-
4.2.3.
INVESTIGATING
SEQUENCE
DATA
FOR
VNTR
7
LOCUS
..............................................
-
78
-
4.2.3.1.
IN
SILICO
ANALYSIS
.........................................................................................
-
78
-
4.2.3.2.
IN
VITRO
ANALYSIS
..........................................................................................
-
81
-
4.2.4.
DEVELOPMENT
OF
MCA
AS
A
MIRU-VNTR
ALLELE
DETERMINING
METHOD
.........
-
86
-
4.2.4.1.
PRIMER
DESIGN
..............................................................................................
-
86
-
4.2.4.2.
CUTOFF-BASED
ALLELE
DETERMINATION
............................................................
-
87
-
4.2.5.
HIERARCHICAL
TYPING
APPROACH
(SNP
+
INMV)
...............................................
-
90
-
4.2.5.1.
SNP
ANALYSIS
(INMV-GUIDED
APPROACH)
...................................................
-
90
-
4.2.5.1.1.
EVALUATION
OF
INMV
TYPES
AS
A
MEANS
FOR
DIFFERENTIATION
BETWEEN
MAP
TYPES
C
AND
S
............................................................................................................
-
90
-
4.2.5.1.2.
PRIORITISATION
OF
SNP
ANALYSIS
FOR
MAP
TYPE
C
.................................
-
93
-
4.2.5.2.
ADDED
VALUE
OF
THE
HIERARCHICAL
TYPING
APPROACH
(SNP
+
INMV)
........
-
97
-
4.2.5.3.
EVALUATION
OF
THE
PHYLOGENETIC
ACCURACY
OF
DIFFERENT
TR
PANEL
COMBINATIONS
-101-
4.3.
GENOTYPIC
CHARACTERISATION
OF
MAP
FIELD
CULTURES
OBTAINED
FROM
DAIRY
HERDS
IN
HESSE,
THURINGIA
AND
SAXONY
............................................................................................
-103
-
4.3.1.
TR-BASED
MAP
GENOTYPING
RESULTS
...............................................................
-103
-
4.3.1.1.
INMV
TYPES
...............................................................................................
-103-
4.3.1.2.
MV
TYPES
....................................................................................................
-104-
4.3.1.3.
SSR
TYPES
...................................................................................................
-105-
CONTENTS
V
4.3.1.4.
COMBINED
TYPES
(ALL
TANDEM
REPEATS)
.....................................................
-105
-
4.3.2.
HIERARCHICAL
TYPING
APPROACH
(SNP
4-
INMV)
..............................................
-106
-
4.3.2.1.
DISTRIBUTION
OF
MAP
PHYLOGENETIC
GROUPS
(SNP
ANALYSIS)
IN
HESSE,
THURINGIA
AND
SAXONY
...............................................................................................
-106
-
4.3.2.2.
POTENTIAL
APPLICATION
OF
SNP
+
INMV
TYPING
IN
EPIDEMIOLOGICAL
TRACING
-
109-
4.3.3.
INTRA-HERD
AND
INTRA-ANIMAL
STRAIN
DIVERSITY
...................................................
-110
-
4.3.3.1.
INTRA-HERD
DIVERSITY
....................................................................................
-110
-
4.3.3.2.
INTRA-ANIMAL
DIVERSITY
..............................................................................
-
111
-
4.4.
MAP
PHENOTYPIC
CHARACTERISATION
USING
FT-IR
...................................................
-ILL-
5.
DISCUSSION
.......................................................................................................................
-114-
5.1.
OPTIMISATION
OF
THE
MAP
GENOTYPING
METHODOLOGY
..........................................
-114-
5.2.
GENOTYPIC
CHARACTERISATION
OF
MAP
FIELD
CULTURES
OBTAINED
FROM
DAIRY
HERDS
IN
HESSE,
THURINGIA
AND
SAXONY
............................................................................................
-122
-
5.3.
MAP
PHENOTYPIC
CHARACTERISATION
USING
FT-IR
AND
CORRELATION
BETWEEN
GENOTYPING
AND
PHENOTYPING
.................................................................................................................
-127
-
6.
CONCLUSION
.......................................................................................................................
-129
-
SUMMARY
.................................................................................................................................
-131
-
ZUSAMMENFASSUNG
..................................................................................................................
-133
-
REFERENCES
...............................................................................................................................
-135
-
ANNEX
......................................................................................................................................
-163
-
ACKNOWLEDGEMENTS
................................................................................................................
-164
-
|
| any_adam_object | 1 |
| author | Mahmoud Fawzy Mohammed, Ahmad |
| author_facet | Mahmoud Fawzy Mohammed, Ahmad |
| author_role | aut |
| author_sort | Mahmoud Fawzy Mohammed, Ahmad |
| author_variant | f m a m fma fmam |
| building | Verbundindex |
| bvnumber | BV046114969 |
| ctrlnum | (OCoLC)1085134963 (DE-599)DNB1176726536 |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV046114969 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:35:40Z |
| institution | BVB |
| institution_GND | (DE-588)1065553803 |
| isbn | 9783835967564 3835967568 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031495490 |
| oclc_num | 1085134963 |
| open_access_boolean | |
| owner | DE-188 |
| owner_facet | DE-188 |
| physical | XIII, 165 Seiten Illustrationen, Diagramme |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| publisher | VVB Laufersweiler Verlag |
| record_format | marc |
| series2 | Edition Scientifique |
| spelling | Mahmoud Fawzy Mohammed, Ahmad Verfasser aut Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany submitted by Ahmad Mahmoud Fawzy Mohammed Gießen, Lahn VVB Laufersweiler Verlag 2019 XIII, 165 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Edition Scientifique Dissertation Justus-Liebig-Universität Gießen 2019 cattle cabt Mycobacterium avium subsp. paratuberculosis cabt epidemiology cabt disease prevalence cabt polymerase chain reaction cabt germany cabt Genotypisierung (DE-588)7641829-7 gnd rswk-swf Mycobacterium paratuberculosis (DE-588)4520169-9 gnd rswk-swf Doktorarbeit Uni Wissenschaft (DE-588)4113937-9 Hochschulschrift gnd-content Mycobacterium paratuberculosis (DE-588)4520169-9 s Genotypisierung (DE-588)7641829-7 s DE-604 VVB LAUFERSWEILER Verlag (DE-588)1065553803 pbl DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031495490&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mahmoud Fawzy Mohammed, Ahmad Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany cattle cabt Mycobacterium avium subsp. paratuberculosis cabt epidemiology cabt disease prevalence cabt polymerase chain reaction cabt germany cabt Genotypisierung (DE-588)7641829-7 gnd Mycobacterium paratuberculosis (DE-588)4520169-9 gnd |
| subject_GND | (DE-588)7641829-7 (DE-588)4520169-9 (DE-588)4113937-9 |
| title | Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany |
| title_auth | Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany |
| title_exact_search | Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany |
| title_full | Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany submitted by Ahmad Mahmoud Fawzy Mohammed |
| title_fullStr | Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany submitted by Ahmad Mahmoud Fawzy Mohammed |
| title_full_unstemmed | Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany submitted by Ahmad Mahmoud Fawzy Mohammed |
| title_short | Genotypic and phenotypic characterisation of Mycobacterium avium subsp. paratuberculosis (MAP) cultures from different states of Germany |
| title_sort | genotypic and phenotypic characterisation of mycobacterium avium subsp paratuberculosis map cultures from different states of germany |
| topic | cattle cabt Mycobacterium avium subsp. paratuberculosis cabt epidemiology cabt disease prevalence cabt polymerase chain reaction cabt germany cabt Genotypisierung (DE-588)7641829-7 gnd Mycobacterium paratuberculosis (DE-588)4520169-9 gnd |
| topic_facet | cattle Mycobacterium avium subsp. paratuberculosis epidemiology disease prevalence polymerase chain reaction germany Genotypisierung Mycobacterium paratuberculosis Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031495490&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mahmoudfawzymohammedahmad genotypicandphenotypiccharacterisationofmycobacteriumaviumsubspparatuberculosismapculturesfromdifferentstatesofgermany AT vvblaufersweilerverlag genotypicandphenotypiccharacterisationofmycobacteriumaviumsubspparatuberculosismapculturesfromdifferentstatesofgermany |