Holdings: Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin

Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin:

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Bibliographic Details
Main Author:	Bremser, Stephan (Author)
Format:	Thesis Book
Language:	English
Published:	Köln 2019
Subjects:	Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Physical Description:	VIII, 71 Seiten Illustrationen, Diagramme 21 cm

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adam_text	CONTENTS ABBREVIATIONS III ABSTRACT V ZUSAMMENFASSUNG VII 1 INTRODUCTION 1 1.1 ANATOMY OF THE LOCUS COERULEUS ..................................................................... 2 1.2 THE NEUROTRANSMITTER NORADRENALINE AND ITS RECEPTORS ................................. 4 1.3 THE ELECTROPHYSIOLOGY OF LOCUS COERULEUS NEURONS ....................................... 5 1.4 THE FUNCTIONAL INTERCONNECTION OF THE NORADRENERGIC LOCUS COERULEUS AND THE SEROTONERGIC DORSAL RAPHE NUCLEUS ................................. 7 1.5 METHODOLOGICAL APPROACH ................................................................................. 10 1.6 THESIS OBJECTIVES ................................................................................................ 12 2 MATERIALS AND METHODS 13 2.1 CHEMICALS ............................................................................................................ 13 2.2 SOLUTIONS ............................................................................................................ 15 2.3 ANIMAL CARE ...................................................................................................... 16 2.4 BRAIN SLICE PREPARATION .................................................................................... 16 2.5 PATCH CLAMP EXPERIMENTS ................................................................................. 16 2.5.1 WHOLE CELL PATCH CLAMP EXPERIMENTS (7 A VOLTAGE CLAMP MEASURE MENTS) ...................................................................................................... 17 2.5.2 PERFORATED PATCH CLAMP EXPERIMENTS (CURRENT CLAMP MEASUREMENTS) 18 2.5.3 PERFORATED PATCH CLAMP EXPERIMENTS (7 SK VOLTAGE CLAMP MEASURE MENTS) ...................................................................................................... 19 2.5.4 PERFORATED FURA-2 PATCH CLAMP EXPERIMENTS (CA 2+ IMAGING) .... 19 2.6 PHARMACOLOGICAL EXPERIMENTS ............................................................................... 20 2.7 DATA ANALYSIS .......................................................................................................... 20 2.7.1 7 A ISOLATION ................................................................................................. 20 2.7.2 FREQUENCY AND PRECISION ............................................................................ 21 2.7.3 ACTION POTENTIAL PARAMETERS ................................................................... 21 2.7.4 INPUT RESISTANCE ........................................................................................ 21 2.7.5 EXCITABILITY ................................................................................................. 22 2.7.6 SLOW AFTERHYPERPOLARISATION ...................................................................... 22 2.7.7 DELAYED EXCITABILITY .................................................................................. 22 2.7.8 7 SK MEASUREMENT ..................................................................................... 23 2.7.9 CA 2+ TRANSIENTS ........................................................................................ 23 2.8 STATISTICS ................................................................................................................ 23 2.9 IMMUNOHISTOCHEMISTRY ........................................................................................... 24 2.10 IMAGE PROCESSING .................................................................................................... 24 I 3 RESULTS 25 3.1 ELECTROPHYSIOLOGICAL CHARACTERISATION OF MOUSE LOCUS COERULEUS NEURONS . 25 3.1.1 LOCUS COERULEUS NEURONS ARE ENDOGENOUS PACEMAKER ......................... 26 3.1.2 LOCUS COERULEUS NEURONS ARE DISTINGUISHABLE BY THE SHAPE OF THEIR ACTION POTENTIALS ........................................................................................ 27 3.1.3 ALL LOCUS COERULEUS NEURONS SHOW A SLOW AFTERHYPERPOLARISATION . . 30 3.1.4 THE DELAYED EXCITABILITY AFTER HYPERPOLARISATION OF LC NEURONS . . 30 3.2 THE MODULATION OF LOCUS COERULEUS NEURONS BY 5-HT ..................................... 35 3.2.1 5-HT INHIBITS LC NEURONS IN A CONCENTRATION DEPENDENT MANNER . 35 3.2.2 LC NEURONS ARE STRONGLY INHIBITED BY 5-HT ........................................... 36 3.2.3 NO CROSS-REACTIVITY OF 5-HT WITH ADRENERGIC RECEPTORS IN HIGH CON CENTRATIONS ................................................................................................. 36 3.2.4 5-HT MODULATES THE EXCITABILITY OF LC NEURONS .................................. 37 3.2.5 5-HT MODULATES CALCIUM CURRENTS .......................................................... 41 3.2.6 5-HT MODULATES THE I SK ............................................................................ 44 4 DISCUSSION 47 4.1 CHARACTERISATION OF THE INTRINSIC ELECTROPHYSIOLOGICAL MEMBRANE PROPERTIES OF MOUSE LOCUS COERULEUS NEURONS ...................................................................... 47 4.1.1 LOCUS COERULEUS NEURONS ARE INTRINSIC PACEMAKER ............................... 47 4.1.2 LOCUS COERULEUS NEURONS SHOW DIFFERENT TYPES OF ACTION POTENTIALS 48 4.1.3 THE SLOW AFTERHYPERPOLARISATION IS MEDIATED BY AN KC A CONDUCTANCE 48 4.1.4 THE 7 A CONTRIBUTES TO THE DELAYED EXCITABILITY ..................................... 50 4.1.5 FUNCTIONAL RELEVANCE OF THE SLOW AFTERHYPERPOLARISATION AND THE DE LAYED EXCITABILITY IN LOCUS COERULEUS NEURONS ..................................... 50 4.2 THE MODULATION OF THE CELL INTRINSIC ELECTROPHYSIOLOGICAL PROPERTIES OF LO CUS COERULEUS NEURONS BY 5-HT ............................................................................ 51 4.2.1 5-HT BI-DIRECTIONALLY MODULATES LOCUS COERULEUS NEURONS ............... 51 4.2.2 THE REDUCTION OF THE EXCITABILITY BY 5-HT MIGHT BE MEDIATED BY GIRK CHANNELS ........................................................................................... 52 4.2.3 THE INCREASE OF THE EXCITABILITY BY 5-HT IS MEDIATED BY CA 2+ CURRENTS 52 4.2.4 5-HT REDUCES THE CA 2+ INFLUX DURING SUSTAINED EXCITATION ............ 53 4.2.5 5-HT MODULATES THE INTRINSIC SNR OF INDIVIDUAL LC NEURONS .... 54 4.3 OUTLOOK ................................................................................................................... 55 5 APPENDIX 57 LIST OF FIGURES 58 LIST OF TABLES 59 BIBLIOGRAPHY 60 DANKSAGUNG 69 ERKLARUNG 70 PART-PUBLICATIONS / TEILPUBLIKATIONEN 71 II
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spelling	Bremser, Stephan Verfasser (DE-588)1179201248 aut Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin vorgelegt von Stephan Bremser aus Köln Köln 2019 VIII, 71 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität zu Köln 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1184816646/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031448361&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Bremser, Stephan Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin
subject_GND	(DE-588)4113937-9
title	Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin
title_auth	Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin
title_exact_search	Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin
title_full	Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin vorgelegt von Stephan Bremser aus Köln
title_fullStr	Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin vorgelegt von Stephan Bremser aus Köln
title_full_unstemmed	Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin vorgelegt von Stephan Bremser aus Köln
title_short	Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin
title_sort	intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin
topic_facet	Hochschulschrift
url	http://d-nb.info/1184816646/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031448361&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT bremserstephan intrinsicmembranepropertiesoflocuscoeruleusneuronsandtheirmodulationbyserotonin

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