Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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Köln
2019
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| Beschreibung: | VIII, 71 Seiten Illustrationen, Diagramme 21 cm |
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MARC
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| 100 | 1 | |a Bremser, Stephan |e Verfasser |0 (DE-588)1179201248 |4 aut | |
| 245 | 1 | 0 | |a Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin |c vorgelegt von Stephan Bremser aus Köln |
| 264 | 1 | |a Köln |c 2019 | |
| 300 | |a VIII, 71 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Universität zu Köln |d 2018 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-031448361 | ||
Datensatz im Suchindex
| _version_ | 1804180348911747072 |
|---|---|
| adam_text | CONTENTS
ABBREVIATIONS
III
ABSTRACT
V
ZUSAMMENFASSUNG
VII
1
INTRODUCTION
1
1.1
ANATOMY
OF
THE
LOCUS
COERULEUS
.....................................................................
2
1.2
THE
NEUROTRANSMITTER
NORADRENALINE
AND
ITS
RECEPTORS
.................................
4
1.3
THE
ELECTROPHYSIOLOGY
OF
LOCUS
COERULEUS
NEURONS
.......................................
5
1.4
THE
FUNCTIONAL
INTERCONNECTION
OF
THE
NORADRENERGIC
LOCUS
COERULEUS
AND
THE
SEROTONERGIC
DORSAL
RAPHE
NUCLEUS
.................................
7
1.5
METHODOLOGICAL
APPROACH
.................................................................................
10
1.6
THESIS
OBJECTIVES
................................................................................................
12
2
MATERIALS
AND
METHODS
13
2.1
CHEMICALS
............................................................................................................
13
2.2
SOLUTIONS
............................................................................................................
15
2.3
ANIMAL
CARE
......................................................................................................
16
2.4
BRAIN
SLICE
PREPARATION
....................................................................................
16
2.5
PATCH
CLAMP
EXPERIMENTS
.................................................................................
16
2.5.1
WHOLE
CELL
PATCH
CLAMP
EXPERIMENTS
(7
A
VOLTAGE
CLAMP
MEASURE
MENTS)
......................................................................................................
17
2.5.2
PERFORATED
PATCH
CLAMP
EXPERIMENTS
(CURRENT
CLAMP
MEASUREMENTS)
18
2.5.3
PERFORATED
PATCH
CLAMP
EXPERIMENTS
(7
SK
VOLTAGE
CLAMP
MEASURE
MENTS)
......................................................................................................
19
2.5.4
PERFORATED
FURA-2
PATCH
CLAMP
EXPERIMENTS
(CA
2+
IMAGING)
....
19
2.6
PHARMACOLOGICAL
EXPERIMENTS
...............................................................................
20
2.7
DATA
ANALYSIS
..........................................................................................................
20
2.7.1
7
A
ISOLATION
.................................................................................................
20
2.7.2
FREQUENCY
AND
PRECISION
............................................................................
21
2.7.3
ACTION
POTENTIAL
PARAMETERS
...................................................................
21
2.7.4
INPUT
RESISTANCE
........................................................................................
21
2.7.5
EXCITABILITY
.................................................................................................
22
2.7.6
SLOW
AFTERHYPERPOLARISATION
......................................................................
22
2.7.7
DELAYED
EXCITABILITY
..................................................................................
22
2.7.8
7
SK
MEASUREMENT
.....................................................................................
23
2.7.9
CA
2+
TRANSIENTS
........................................................................................
23
2.8
STATISTICS
................................................................................................................
23
2.9
IMMUNOHISTOCHEMISTRY
...........................................................................................
24
2.10
IMAGE
PROCESSING
....................................................................................................
24
I
3
RESULTS
25
3.1
ELECTROPHYSIOLOGICAL
CHARACTERISATION
OF
MOUSE
LOCUS
COERULEUS
NEURONS
.
25
3.1.1
LOCUS
COERULEUS
NEURONS
ARE
ENDOGENOUS
PACEMAKER
.........................
26
3.1.2
LOCUS
COERULEUS
NEURONS
ARE
DISTINGUISHABLE
BY
THE
SHAPE
OF
THEIR
ACTION
POTENTIALS
........................................................................................
27
3.1.3
ALL
LOCUS
COERULEUS
NEURONS
SHOW
A
SLOW
AFTERHYPERPOLARISATION
.
.
30
3.1.4
THE
DELAYED
EXCITABILITY
AFTER
HYPERPOLARISATION
OF
LC
NEURONS
.
.
30
3.2
THE
MODULATION
OF
LOCUS
COERULEUS
NEURONS
BY
5-HT
.....................................
35
3.2.1
5-HT
INHIBITS
LC
NEURONS
IN
A
CONCENTRATION
DEPENDENT
MANNER
.
35
3.2.2
LC
NEURONS
ARE
STRONGLY
INHIBITED
BY
5-HT
...........................................
36
3.2.3
NO
CROSS-REACTIVITY
OF
5-HT
WITH
ADRENERGIC
RECEPTORS
IN
HIGH
CON
CENTRATIONS
.................................................................................................
36
3.2.4
5-HT
MODULATES
THE
EXCITABILITY
OF
LC
NEURONS
..................................
37
3.2.5
5-HT
MODULATES
CALCIUM
CURRENTS
..........................................................
41
3.2.6
5-HT
MODULATES
THE
I
SK
............................................................................
44
4
DISCUSSION
47
4.1
CHARACTERISATION
OF
THE
INTRINSIC
ELECTROPHYSIOLOGICAL
MEMBRANE
PROPERTIES
OF
MOUSE
LOCUS
COERULEUS
NEURONS
......................................................................
47
4.1.1
LOCUS
COERULEUS
NEURONS
ARE
INTRINSIC
PACEMAKER
...............................
47
4.1.2
LOCUS
COERULEUS
NEURONS
SHOW
DIFFERENT
TYPES
OF
ACTION
POTENTIALS
48
4.1.3
THE
SLOW
AFTERHYPERPOLARISATION
IS
MEDIATED
BY
AN
KC
A
CONDUCTANCE
48
4.1.4
THE
7
A
CONTRIBUTES
TO
THE
DELAYED
EXCITABILITY
.....................................
50
4.1.5
FUNCTIONAL
RELEVANCE
OF
THE
SLOW
AFTERHYPERPOLARISATION
AND
THE
DE
LAYED
EXCITABILITY
IN
LOCUS
COERULEUS
NEURONS
.....................................
50
4.2
THE
MODULATION
OF
THE
CELL
INTRINSIC
ELECTROPHYSIOLOGICAL
PROPERTIES
OF
LO
CUS
COERULEUS
NEURONS
BY
5-HT
............................................................................
51
4.2.1
5-HT
BI-DIRECTIONALLY
MODULATES
LOCUS
COERULEUS
NEURONS
...............
51
4.2.2
THE
REDUCTION
OF
THE
EXCITABILITY
BY
5-HT
MIGHT
BE
MEDIATED
BY
GIRK
CHANNELS
...........................................................................................
52
4.2.3
THE
INCREASE
OF
THE
EXCITABILITY
BY
5-HT
IS
MEDIATED
BY
CA
2+
CURRENTS
52
4.2.4
5-HT
REDUCES
THE
CA
2+
INFLUX
DURING
SUSTAINED
EXCITATION
............
53
4.2.5
5-HT
MODULATES
THE
INTRINSIC
SNR
OF
INDIVIDUAL
LC
NEURONS
....
54
4.3
OUTLOOK
...................................................................................................................
55
5
APPENDIX
57
LIST
OF
FIGURES
58
LIST
OF
TABLES
59
BIBLIOGRAPHY
60
DANKSAGUNG
69
ERKLARUNG
70
PART-PUBLICATIONS
/
TEILPUBLIKATIONEN
71
II
|
| any_adam_object | 1 |
| author | Bremser, Stephan |
| author_GND | (DE-588)1179201248 |
| author_facet | Bremser, Stephan |
| author_role | aut |
| author_sort | Bremser, Stephan |
| author_variant | s b sb |
| building | Verbundindex |
| bvnumber | BV046067128 |
| ctrlnum | (OCoLC)1111882880 (DE-599)DNB1184816646 |
| discipline | Medizin |
| format | Thesis Book |
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| indexdate | 2024-07-10T08:34:20Z |
| institution | BVB |
| language | English |
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| physical | VIII, 71 Seiten Illustrationen, Diagramme 21 cm |
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| spelling | Bremser, Stephan Verfasser (DE-588)1179201248 aut Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin vorgelegt von Stephan Bremser aus Köln Köln 2019 VIII, 71 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität zu Köln 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1184816646/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031448361&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bremser, Stephan Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin |
| subject_GND | (DE-588)4113937-9 |
| title | Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin |
| title_auth | Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin |
| title_exact_search | Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin |
| title_full | Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin vorgelegt von Stephan Bremser aus Köln |
| title_fullStr | Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin vorgelegt von Stephan Bremser aus Köln |
| title_full_unstemmed | Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin vorgelegt von Stephan Bremser aus Köln |
| title_short | Intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin |
| title_sort | intrinsic membrane properties of locus coeruleus neurons and their modulation by serotonin |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1184816646/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031448361&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bremserstephan intrinsicmembranepropertiesoflocuscoeruleusneuronsandtheirmodulationbyserotonin |
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