Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Ulm
2018
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 92 Seiten Illustrationen, Diagramme 30 cm |
Internformat
MARC
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| 100 | 1 | |a Commisso, Barbara |e Verfasser |0 (DE-588)1181826233 |4 aut | |
| 245 | 1 | 0 | |a Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 |c Barbara Commisso, Vasto/Italy |
| 264 | 1 | |a Ulm |c 2018 | |
| 300 | |a 92 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Universität Ulm |d 2018 | ||
| 653 | |a Amyotrophic lateral sclerosis | ||
| 653 | |a Mice | ||
| 653 | |a Motor cortex | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 710 | 2 | |a Universität Ulm |0 (DE-588)30607-1 |4 dgg | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-031444499 | ||
Datensatz im Suchindex
| _version_ | 1804180341659795456 |
|---|---|
| adam_text | T
ABLE
OF
CONTENTS
LIST
OF
ABBREVIATIONS
......................................................................................................
1
1.
INTRODUCTION.
..............................................................................................................
5
1.1
AMYOTROPHIC
LATERAL
SCLEROSIS:
EPIDEMIOLOGY
&
HISTORY
.................................
5
1.2
ETIOLOGY
OF
ALS
..........................................................................................................
7
1.3
NEURONAL
NETWORK
PERSPECTIVE
.............................................................................
9
1.4
THE
MOTOR
NETWORK
...............................................................................................
10
1.5
NEUROIMAGING
TECHNIQUES
...................................................................................
13
1.6
MRIINALS
...............................................................................................................
15
1.7
ADENO-
ASSOCIATED
VIRUS
FOR
NETWORK
INVESTIGATION
..........................
18
1.8
ALS
ANIMAL
MODEL:
SOD1-G93A...........................................................................
21
1.9
AIM
OF
THE
THESIS
....................................................................................................
23
2.
MATERIAL
......................................................................................................................
24
2.1
HARDWARE
................................................................................................................
24
2.2
IN
SILICO
TOOLS
..........................................................................................................
25
2.3
ANTIBODIES
...............................................................................................................
25
2.4
CHEMICALS,
DRUGS
AND
MATERIALS
..........
..............................................................
25
2.5
REAGENTS
FOR
IMMUNOSTAINING
............................................................................
27
2.6
REAGENTS
FOR
VIRAL
PRODUCTION
............................................................................
27
2.7
VIRUSES AND
TRACER
.................................................................................................
29
2.8
PRIMERS
...................................................................................................................
30
2.9
ORGANISMS
...............................................................................................................
30
3.
METHODS
.......................................................................................................................
31
3.1
ADENO
ASSOCIATED
VIRUSES
PRODUCTION
..............................................................
31
3.3
INTRACEREBRAL
INJECTIONS
.......................................................................................
38
3.3
PERFUSION
.................................................................................................................
39
3.4
IMMUNOHISTOCHEMISTRY
.......................................................................................
40
3.5
IMAGE
ACQUISITION
..................................................................................................
41
3.6
IMAGE
ANALYSIS
.......................................................................................................
42
3.7
STATISTICAL
ANALYSIS
.................................................................................................
44
4.
RESULTS
............................................
46
3
4.1
IN-
VIVO
ANALYSIS
PROVE
RAAV9-SLR
TO
BE
THE
MOST
EFFICIENT
VIRAL
TOOL
FOR
RETROGRADE
CONNECTIVITY
TRACING
46
49
4.2
MAPPING
MOTOR
CORTEX
LONG-
RANGE
INPUT
CONNECTIVITY
VIA
AAV9-SLR
49
4.3
PMO
INPUT
CONNECTIVITY
IS
ALREADY
ALTERED
IN
EARLY-PRESYMPTOMATIC
MSOD
55
4.4
PMO
INPUT
HYPERCONNECTIVITY
IS
DOUBLE
PROVEN
AND
SPECIFIC
FOR
THE
MOTOR
NETWORK
60
4.5
INPUT
HYPERCONNECTIVITY
IN
MSOD
PROGRESSIVELY
AFFECTS
CORTICAL
AND
SUBCORTICAL
STRUCTURES
DURING
DISEASE
PROGRESSION
62
64
4.6
CORTICAL
PYRAMIDAL
NEURONS
PART
OF
THE
MOTOR
SUBNETWORK
PRESENT
SIMULTANEOUS
LOSS
OF
SPINES
ON
BASAL
DENDRITES
......................
65
4.7
MISFOLDED
SOD
EXPRESSION
IS
NOT
ENRICHED
IN
NEURONS
PROJECTING
TO
PMO
68
71
4.8
ALTERED
PMO
OUTPUT
LONG-
RANGE
CONNECTIVITY
AT
EARLY-PRESYMPTOMATIC
MSOD
.........................................................
72
5.
DISCUSSION
74
5.1
RECOMBINANT
AAV9
AS
THE
MOST
APPROPRIATE
TOOL
.......................
74
5.2
CLUES
FROM
SPINE
DENSITY
75
5.3
THE
MEANING
OF
HYPER
CONNECTIVITY
..........................
75
5.4
LIMITATIONS
................................................................
77
5.5
CONCLUSION 78
6.
SUMMARY
79
7.
LIST
OF
REFERENCE
80
S.
DECLARATION
ON
OATH
92
4
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| author | Commisso, Barbara |
| author_GND | (DE-588)1181826233 |
| author_facet | Commisso, Barbara |
| author_role | aut |
| author_sort | Commisso, Barbara |
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| building | Verbundindex |
| bvnumber | BV046063192 |
| ctrlnum | (OCoLC)1111893790 (DE-599)KXP1662417330 |
| discipline | Medizin |
| format | Thesis Book |
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| indexdate | 2024-07-10T08:34:13Z |
| institution | BVB |
| institution_GND | (DE-588)30607-1 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031444499 |
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| physical | 92 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2018 |
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| spelling | Commisso, Barbara Verfasser (DE-588)1181826233 aut Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 Barbara Commisso, Vasto/Italy Ulm 2018 92 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2018 Amyotrophic lateral sclerosis Mice Motor cortex (DE-588)4113937-9 Hochschulschrift gnd-content Universität Ulm (DE-588)30607-1 dgg B:DE-101 application/pdf http://d-nb.info/1188066455/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031444499&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Commisso, Barbara Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 |
| subject_GND | (DE-588)4113937-9 |
| title | Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 |
| title_auth | Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 |
| title_exact_search | Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 |
| title_full | Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 Barbara Commisso, Vasto/Italy |
| title_fullStr | Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 Barbara Commisso, Vasto/Italy |
| title_full_unstemmed | Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 Barbara Commisso, Vasto/Italy |
| title_short | Stage-dependent hyperconnectivity to primary motor cortex in ALS mouse model revealed via retrograde- AAV9 |
| title_sort | stage dependent hyperconnectivity to primary motor cortex in als mouse model revealed via retrograde aav9 |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1188066455/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031444499&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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