Holdings: Interactions of cadherins and catenins in Schwann cell/fibroblast cultures

Interactions of cadherins and catenins in Schwann cell/fibroblast cultures:

Saved in:

Bibliographic Details
Main Author:	Chikobava, Levan 1987- (Author)
Format:	Thesis Book
Language:	English
Published:	Münster 2018
Subjects:	Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Physical Description:	50 Blätter Illustrationen 30 cm

Staff View

MARC


LEADER	00000nam a2200000 c 4500
001	BV046059813
003	DE-604
005	00000000000000.0
007	t
008	190723s2018 gw a\|\|\| m\|\|\| 00\|\|\| eng d
015			\|a 19,H02 \|2 dnb
016	7		\|a 1173938907 \|2 DE-101
020			\|c Broschur
035			\|a (OCoLC)1110066531
035			\|a (DE-599)DNB1173938907
040			\|a DE-604 \|b ger \|e rda
041	0		\|a eng
044			\|a gw \|c XA-DE
049			\|a DE-355
084			\|a 610 \|2 sdnb
084			\|a 570 \|2 sdnb
100	1		\|a Chikobava, Levan \|d 1987- \|e Verfasser \|0 (DE-588)1174654449 \|4 aut
245	1	0	\|a Interactions of cadherins and catenins in Schwann cell/fibroblast cultures \|c vorgelegt con Chikobava, Levan
264		1	\|a Münster \|c 2018
300			\|a 50 Blätter \|b Illustrationen \|c 30 cm
336			\|b txt \|2 rdacontent
337			\|b n \|2 rdamedia
338			\|b nc \|2 rdacarrier
502			\|b Dissertation \|c Westfälische Wilhelms-Universität Münster \|d 2018 \|g Beschränkt für den Austausch
655		7	\|0 (DE-588)4113937-9 \|a Hochschulschrift \|2 gnd-content
856	4	2	\|m B:DE-101 \|q application/pdf \|u http://d-nb.info/1173938907/04 \|3 Inhaltsverzeichnis
856	4	2	\|m DNB Datenaustausch \|q application/pdf \|u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031441157&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA \|3 Inhaltsverzeichnis
999			\|a oai:aleph.bib-bvb.de:BVB01-031441157

Record in the Search Index

_version_	1804180334321860608
adam_text	TABLE OF CONTENTS 1. INTRODUCTION.................................................................................................................1 1.1 STRUCTURE OF THE INTACT PERIPHERAL N ERVE......................................................................................1 1.2 ORIGIN OF SCHWANN CELLS IN PERIPHERAL NERVOUS SYSTEM ...........................................................3 1.3 PERIPHERAL NERVE INJURY...................................................................................................................4 1.4 MECHANISMS AND FUNCTIONS OF EPH AND EPHRIN SIGNALLING ........................................................ 6 2. MATERIALS AND METHODS............................................................................................ 9 2.1 CELL CULTURE METHODS..................................................................................................................... 9 2.1.1 PURIFICATION OF RAT SCHWANN CELLS FROM PERIPHERAL NERVES..............................................9 2.1.2 CELL CULTURE.............................................................................................................................. 9 2.1.3 MIGRATION ASSAYS..................................................................................................................11 2.2 IMMUNOLOGICAL STAINING............................................................................................................... 11 2.2.1 IMMUNOCYTOCHEMISTRY........................................................................................................ 11 2.2.2 IMAGE ANALYSIS AND QUANTITATION.......................................................................................14 2.3 PROTEIN BIOCHEMICAL METHODS....................................................................................................14 2.3.1 ISOLATION OF TOTAL PROTEIN FROM CELLS................................................................................... 14 2.3.2 QUANTITATION OF WHOLE PROTEIN EXTRACTS USING PIERCE BCA PROTEIN ASSAY .................. 15 2.3.3 SEPARATION OF PROTEINS BY ELECTROPHORETIC MOBILITY (SDS-PAGE) ............................... 15 2.3.4 PROTEIN TRANSFER AND DETECTION (WESTERN BLOT ANALYSIS) ................................................ 17 2.3.5 CO-IMMUNOPRECIPITATION.....................................................................................................19 3. RESULTS 20 3.1 IN THE PRESENCE OF FIBROBLASTS SCHWANN CELLS SHOW INCREASED EXPRESSION OF N-CADHERIN AND CATENINS............................................................................................................................................20 3.2 N-CADHERIN AND CATENINS ARE LOCALISED IN CELL-CELL CONTACT REGIONS AND CELL MEMBRANES IN SCHWANN CELLS CULTURED WITH FIBROBLASTS.........................................................................................23 3.3 N-CADHERIN IS ASSOCIATED WITH CATENINS IN TWO DIFFERENT COMPLEXES IN SCHWANN CELLS..25 3.4 SCHWANN CELLS TREATED WITH SNEW SHOW DEFECTIVE ACTIN POLYMERISATION AND FAIL TO ESTABLISH GROUPS...................................................................................................................................... 27 3.5 CO-CULTURED SCHWANN CELLS AND FIBROBLASTS SHOW HIGHER RATES OF MIGRATION THAN MONOCULTURES OF SCHWANN CELLS OR FIBROBLASTS................................................................................... 28 4. DISCUSSION............................................................................................................... 29 5. APPENDIX.................................................................................................................. 33 5.1 UNITS...............................................................................................................................................33 5.2 EQUIPMENT AND DEVICES.............................................................................................................. 35 5.3 TABLE DIRECTORY............................................................................................................................. 36 5.4 FIGURE DIRECTORY............................................................................................................................37 6. REFERENCES............................................................................................................... 38 7. CURRICULUM VITAE......................................................................................................47 8. DANKSAGUNG.............................................................................................................50
any_adam_object	1
author	Chikobava, Levan 1987-
author_GND	(DE-588)1174654449
author_facet	Chikobava, Levan 1987-
author_role	aut
author_sort	Chikobava, Levan 1987-
author_variant	l c lc
building	Verbundindex
bvnumber	BV046059813
ctrlnum	(OCoLC)1110066531 (DE-599)DNB1173938907
discipline	Biologie Medizin
format	Thesis Book
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01421nam a2200361 c 4500</leader><controlfield tag="001">BV046059813</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">190723s2018 gw a\|\|\| m\|\|\| 00\|\|\| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">19,H02</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1173938907</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Broschur</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1110066531</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1173938907</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">570</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Chikobava, Levan</subfield><subfield code="d">1987-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1174654449</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Interactions of cadherins and catenins in Schwann cell/fibroblast cultures</subfield><subfield code="c">vorgelegt con Chikobava, Levan</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Münster</subfield><subfield code="c">2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">50 Blätter</subfield><subfield code="b">Illustrationen</subfield><subfield code="c">30 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Westfälische Wilhelms-Universität Münster</subfield><subfield code="d">2018</subfield><subfield code="g">Beschränkt für den Austausch</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://d-nb.info/1173938907/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031441157&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-031441157</subfield></datafield></record></collection>
genre	(DE-588)4113937-9 Hochschulschrift gnd-content
genre_facet	Hochschulschrift
id	DE-604.BV046059813
illustrated	Illustrated
indexdate	2024-07-10T08:34:06Z
institution	BVB
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-031441157
oclc_num	1110066531
open_access_boolean
owner	DE-355 DE-BY-UBR
owner_facet	DE-355 DE-BY-UBR
physical	50 Blätter Illustrationen 30 cm
publishDate	2018
publishDateSearch	2018
publishDateSort	2018
record_format	marc
spelling	Chikobava, Levan 1987- Verfasser (DE-588)1174654449 aut Interactions of cadherins and catenins in Schwann cell/fibroblast cultures vorgelegt con Chikobava, Levan Münster 2018 50 Blätter Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Westfälische Wilhelms-Universität Münster 2018 Beschränkt für den Austausch (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1173938907/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031441157&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Chikobava, Levan 1987- Interactions of cadherins and catenins in Schwann cell/fibroblast cultures
subject_GND	(DE-588)4113937-9
title	Interactions of cadherins and catenins in Schwann cell/fibroblast cultures
title_auth	Interactions of cadherins and catenins in Schwann cell/fibroblast cultures
title_exact_search	Interactions of cadherins and catenins in Schwann cell/fibroblast cultures
title_full	Interactions of cadherins and catenins in Schwann cell/fibroblast cultures vorgelegt con Chikobava, Levan
title_fullStr	Interactions of cadherins and catenins in Schwann cell/fibroblast cultures vorgelegt con Chikobava, Levan
title_full_unstemmed	Interactions of cadherins and catenins in Schwann cell/fibroblast cultures vorgelegt con Chikobava, Levan
title_short	Interactions of cadherins and catenins in Schwann cell/fibroblast cultures
title_sort	interactions of cadherins and catenins in schwann cell fibroblast cultures
topic_facet	Hochschulschrift
url	http://d-nb.info/1173938907/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031441157&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT chikobavalevan interactionsofcadherinsandcateninsinschwanncellfibroblastcultures

Holdings

There is no print copy available.

Interlibrary loan Place Request Caution: Not in THWS collection! Indexes
Indexes

MARC

Record in the Search Index

There is no print copy available.

Similar Items