Interactions of cadherins and catenins in Schwann cell/fibroblast cultures:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Münster
2018
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 50 Blätter Illustrationen 30 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV046059813 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 190723s2018 gw a||| m||| 00||| eng d | ||
| 015 | |a 19,H02 |2 dnb | ||
| 016 | 7 | |a 1173938907 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1110066531 | ||
| 035 | |a (DE-599)DNB1173938907 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 610 |2 sdnb | ||
| 084 | |a 570 |2 sdnb | ||
| 100 | 1 | |a Chikobava, Levan |d 1987- |e Verfasser |0 (DE-588)1174654449 |4 aut | |
| 245 | 1 | 0 | |a Interactions of cadherins and catenins in Schwann cell/fibroblast cultures |c vorgelegt con Chikobava, Levan |
| 264 | 1 | |a Münster |c 2018 | |
| 300 | |a 50 Blätter |b Illustrationen |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Westfälische Wilhelms-Universität Münster |d 2018 |g Beschränkt für den Austausch | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u http://d-nb.info/1173938907/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031441157&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-031441157 | ||
Datensatz im Suchindex
| _version_ | 1804180334321860608 |
|---|---|
| adam_text | TABLE OF CONTENTS
1.
INTRODUCTION.................................................................................................................1
1.1 STRUCTURE OF THE INTACT PERIPHERAL N
ERVE......................................................................................1
1.2 ORIGIN OF SCHWANN CELLS IN PERIPHERAL NERVOUS SYSTEM
...........................................................3
1.3 PERIPHERAL NERVE
INJURY...................................................................................................................4
1.4 MECHANISMS AND FUNCTIONS OF EPH AND EPHRIN SIGNALLING
........................................................
6
2. MATERIALS AND
METHODS............................................................................................
9
2.1 CELL CULTURE
METHODS.....................................................................................................................
9
2.1.1 PURIFICATION OF RAT SCHWANN CELLS FROM PERIPHERAL
NERVES..............................................9
2.1.2 CELL
CULTURE..............................................................................................................................
9
2.1.3 MIGRATION
ASSAYS..................................................................................................................11
2.2 IMMUNOLOGICAL
STAINING...............................................................................................................
11
2.2.1
IMMUNOCYTOCHEMISTRY........................................................................................................
11
2.2.2 IMAGE ANALYSIS AND
QUANTITATION.......................................................................................14
2.3 PROTEIN BIOCHEMICAL
METHODS....................................................................................................14
2.3.1 ISOLATION OF TOTAL PROTEIN FROM
CELLS...................................................................................
14
2.3.2 QUANTITATION OF WHOLE PROTEIN EXTRACTS USING PIERCE BCA PROTEIN
ASSAY
..................
15
2.3.3 SEPARATION OF PROTEINS BY ELECTROPHORETIC MOBILITY (SDS-PAGE)
...............................
15
2.3.4 PROTEIN TRANSFER AND DETECTION (WESTERN BLOT ANALYSIS)
................................................
17
2.3.5
CO-IMMUNOPRECIPITATION.....................................................................................................19
3. RESULTS
20
3.1 IN THE PRESENCE OF FIBROBLASTS SCHWANN CELLS SHOW INCREASED
EXPRESSION OF N-CADHERIN
AND
CATENINS............................................................................................................................................20
3.2 N-CADHERIN AND CATENINS ARE LOCALISED IN CELL-CELL CONTACT REGIONS
AND CELL MEMBRANES
IN SCHWANN CELLS CULTURED WITH
FIBROBLASTS.........................................................................................23
3.3 N-CADHERIN IS ASSOCIATED WITH CATENINS IN TWO DIFFERENT COMPLEXES IN
SCHWANN CELLS..25
3.4 SCHWANN CELLS TREATED WITH SNEW SHOW DEFECTIVE ACTIN POLYMERISATION
AND FAIL TO
ESTABLISH
GROUPS......................................................................................................................................
27
3.5 CO-CULTURED SCHWANN CELLS AND FIBROBLASTS SHOW HIGHER RATES OF
MIGRATION THAN
MONOCULTURES OF SCHWANN CELLS OR
FIBROBLASTS...................................................................................
28
4.
DISCUSSION...............................................................................................................
29
5.
APPENDIX..................................................................................................................
33
5.1
UNITS...............................................................................................................................................33
5.2 EQUIPMENT AND
DEVICES..............................................................................................................
35
5.3 TABLE
DIRECTORY.............................................................................................................................
36
5.4 FIGURE
DIRECTORY............................................................................................................................37
6.
REFERENCES...............................................................................................................
38
7. CURRICULUM
VITAE......................................................................................................47
8.
DANKSAGUNG.............................................................................................................50
|
| any_adam_object | 1 |
| author | Chikobava, Levan 1987- |
| author_GND | (DE-588)1174654449 |
| author_facet | Chikobava, Levan 1987- |
| author_role | aut |
| author_sort | Chikobava, Levan 1987- |
| author_variant | l c lc |
| building | Verbundindex |
| bvnumber | BV046059813 |
| ctrlnum | (OCoLC)1110066531 (DE-599)DNB1173938907 |
| discipline | Biologie Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01421nam a2200361 c 4500</leader><controlfield tag="001">BV046059813</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">190723s2018 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">19,H02</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1173938907</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Broschur</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1110066531</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1173938907</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">570</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Chikobava, Levan</subfield><subfield code="d">1987-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1174654449</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Interactions of cadherins and catenins in Schwann cell/fibroblast cultures</subfield><subfield code="c">vorgelegt con Chikobava, Levan</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Münster</subfield><subfield code="c">2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">50 Blätter</subfield><subfield code="b">Illustrationen</subfield><subfield code="c">30 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Westfälische Wilhelms-Universität Münster</subfield><subfield code="d">2018</subfield><subfield code="g">Beschränkt für den Austausch</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://d-nb.info/1173938907/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031441157&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-031441157</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV046059813 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:34:06Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031441157 |
| oclc_num | 1110066531 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 50 Blätter Illustrationen 30 cm |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| record_format | marc |
| spelling | Chikobava, Levan 1987- Verfasser (DE-588)1174654449 aut Interactions of cadherins and catenins in Schwann cell/fibroblast cultures vorgelegt con Chikobava, Levan Münster 2018 50 Blätter Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Westfälische Wilhelms-Universität Münster 2018 Beschränkt für den Austausch (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1173938907/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031441157&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chikobava, Levan 1987- Interactions of cadherins and catenins in Schwann cell/fibroblast cultures |
| subject_GND | (DE-588)4113937-9 |
| title | Interactions of cadherins and catenins in Schwann cell/fibroblast cultures |
| title_auth | Interactions of cadherins and catenins in Schwann cell/fibroblast cultures |
| title_exact_search | Interactions of cadherins and catenins in Schwann cell/fibroblast cultures |
| title_full | Interactions of cadherins and catenins in Schwann cell/fibroblast cultures vorgelegt con Chikobava, Levan |
| title_fullStr | Interactions of cadherins and catenins in Schwann cell/fibroblast cultures vorgelegt con Chikobava, Levan |
| title_full_unstemmed | Interactions of cadherins and catenins in Schwann cell/fibroblast cultures vorgelegt con Chikobava, Levan |
| title_short | Interactions of cadherins and catenins in Schwann cell/fibroblast cultures |
| title_sort | interactions of cadherins and catenins in schwann cell fibroblast cultures |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1173938907/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031441157&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT chikobavalevan interactionsofcadherinsandcateninsinschwanncellfibroblastcultures |
Es ist kein Print-Exemplar vorhanden.
Inhaltsverzeichnis