Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Freiburg im Breisgau
December 2018
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 145 Seiten Illustrationen, Diagramme 30 cm |
Internformat
MARC
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| 100 | 1 | |a Iyer, Shantanu S. |e Verfasser |0 (DE-588)1184841462 |4 aut | |
| 245 | 1 | 0 | |a Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster |c presented by Shantanu S. Iyer |
| 264 | 1 | |a Freiburg im Breisgau |c December 2018 | |
| 300 | |a 145 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Albert-Ludwigs-Universität Freiburg |d 2019 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u http://d-nb.info/1186173165/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031288561&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-031288561 | ||
Datensatz im Suchindex
| _version_ | 1804180073339682816 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
ACKNOWLEDGEMENTS
...... ..........
......4
LIST
OF
FIGURES
.............................................................................................................7
SUMMARY
............................. .......... ......................
........... ............... ..................
10
1
INTRODUCTION
.....................
12
1.1
VARIOUS
MECHANISMS
EXIST
TO
CONTROL
TRANSPOSONS
....................................
16
1.2
TRANSPOSON
CONTROL
MECHANISMS
ARE
EPIGENETIC
IN
NATURE
......................
17
1.3
EPIGENETICS
AND
CHROMATIN
.............................................................................
18
1.3.1
METHYLATION
OF
HISTONE
3
LYSINE
9
................................................................................
22
1.3.2
ACETYLATION
OF
HISTONE
4
LYSINE
16
AND
MOF
............................................................... 24
1.4
THE
MOF-CONTAINING
NSL
COMPLEX
.................................................................
27
1.4.1
THE
NSL
COMPLEX
CONTROLS
HOUSEKEEPING
GENE
EXPRESSION
.......................................
28
1.4.2
THE
NSL
COMPLEX
IMPLICATED
IN
MANY
SCREENS
..........................................................
31
1.5
EPIGENETIC
CONTROL
BY
NON-CODING
RNAS
.......................................................
32
1.6 THE
PIRNA
PATHWAY
.........................................................................................
35
1.6.1
SMALL
RNAS
IN
FLIES
.......................................................................................................
35
1.6.2
OVARY
MORPHOLOGY
.......................................................................................................
37
1.6.3
THE
PRIMARY
PIRNA
PATHWAY
......................................................................................
38
1.6.4
THE
SECONDARY
PATHWAY
..............................................................................................
42
1.6.5
TRANSCRIPTIONAL
GENE
SILENCING
USING
PIRNAS
............................................................44
1.7
THE
PIRNA
PATHWAY
AND
THE
NSL
COMPLEX
....................................................
45
2
AIMS...
......... ..........
47
3
RESULTS
.............................................................................................................
48
3.1
TARGETED
DEPLETION
OF
NSL
COMPLEX
MEMBERS
IN
THE
GERMLINE
CAUSES
WIDESPREAD
TRANSPOSON
UPREGULATION
.......................................................................
48
3.2
SMALL
RNA
SEQUENCING
IDENTIFIES
A
DEFECTING
IN
THE
PRIMARY
PIRNA
BIOGENESIS
.......................................................................................................................
63
3.3
GENERATION
OF
ENDOGENOUSLY
TAGGED
NSL2
USING
CRISER
...........................
73
3.4
NSL1
AND
NSL2
ARE
CHROMATIN
BOUND
AND
BUT
DO
NOT
BIND
PIRNA
CLUSTERS
3.5
A
REEXAMINATION
OF
THE
RNA
SEQUENCING
DATA
REVEALS
THAT
THE
PIRNA
PRECURSOR
TRANSCRIPTS
ARE
DEFECTIVELY
PROCESSED
85
3.6
STAINING
WITH
}2
ANTIBODY,
SPECIFIC
TO
DSRNA,
SHOWS
ACCUMULATION
OF
DSRNA
AT
THE
NUAGE
89
3.7
LOSS
OF
NSL2
IN
THE
GERMLINE CAUSES DESTABILIZATION
AND
LOSS
OF
PIWI
91
3.8
THE
OVARIAN
SOMATIC
SHEATH
CELLS
RECAPITULATE
THE
PIRNA
PATHWAY
PHENOTYPES
OBSERVED
UPON
NSL2
DEPLETION
94
3.9
RNA
SEQUENCING
IN
OSS
CELLS
AFTER
NSL2
RNAI
REVEALS
GENOME
WIDE
TRANSPOSON
UPREGULATION
97
3.10
CHIP
OF
POLII
CONFIRMS
LOSS
OF
TRANSCRIPTIONAL
GENE
SILENCING
99
3.11
SEARCHING
FOR
THE
MOLECULAR
BASIS
OF
PIRNA
PATHWAY
REGULATION
BY
THE
NSL
COMPLEX
106
4
DISCUSSION
ILL
4.1
GENOME-WIDE
UPREGULATION
OF
TRANSPOSONS
112
4.2
ACCUMULATION
OF
PIRNA
PRECURSOR
TRANSCRIPTS 114
4.3
EXAMINING
SIGNATURES
IN
THE
SMALL
RNA
PROFILE
OF
THE
OVARY
115
4.4
THE
NSL
COMPLEX
BINDS
TO
AND
REGULATES
THE
EXPRESSION
OF
ZUC
116
4.5
AN
OVEREXPRESSION
OF
ZUC-GFP
PARTIALLY
RESCUES
THE
PIRNA
PATHWAY
....118
5
MATERIALS
AN
D
METHODS
120
5.1
DROSOPHILA
HUSBANDRY 120
5.2
RNA
ISOLATION
121
5.3
RNA
SEQUENCING
AND
ANALYSIS
121
5.4
QUANTITATIVE
REVERSE
TRANSCRIPTION
PCR
(RT-QPCR)
122
5.5
CHROMATIN
IMMUNOPRECIPITATION
SEQUENCING
(CHLP-SEQ)
123
5.6
CHLP-SEQ
ANALYSIS
125
5.7
SMALL
RNA-SEQUENCING
125
5.8
SMALL
RNA-SEQUENCING
ANALYSIS
126
5.9
IMMUNOFLUORESCENCE
ASSAYS
IN
OVARIES
127
5.10
OSS
CELLS
FLY-CULTURE
128
5.11
RNAI
IN
OSS
CELLS
..............................................................................................
128
5.12
PROTEIN
EXTRACTION
AND
WESTERN
BLOTTING
....................................................
129
6
LIST
OF
ABBREVIATIONS
............................
130
7
REFERENCES
......................................................................................................
134
|
| any_adam_object | 1 |
| author | Iyer, Shantanu S. |
| author_GND | (DE-588)1184841462 |
| author_facet | Iyer, Shantanu S. |
| author_role | aut |
| author_sort | Iyer, Shantanu S. |
| author_variant | s s i ss ssi |
| building | Verbundindex |
| bvnumber | BV045905847 |
| ctrlnum | (OCoLC)1104926478 (DE-599)KXP1663746133 |
| discipline | Biologie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV045905847 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:29:58Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031288561 |
| oclc_num | 1104926478 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 145 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| record_format | marc |
| spelling | Iyer, Shantanu S. Verfasser (DE-588)1184841462 aut Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster presented by Shantanu S. Iyer Freiburg im Breisgau December 2018 145 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Albert-Ludwigs-Universität Freiburg 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1186173165/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031288561&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Iyer, Shantanu S. Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster |
| subject_GND | (DE-588)4113937-9 |
| title | Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster |
| title_auth | Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster |
| title_exact_search | Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster |
| title_full | Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster presented by Shantanu S. Iyer |
| title_fullStr | Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster presented by Shantanu S. Iyer |
| title_full_unstemmed | Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster presented by Shantanu S. Iyer |
| title_short | Characterization of the non-specific lethal (NSL) complex in the germline of Drosophila melanogaster |
| title_sort | characterization of the non specific lethal nsl complex in the germline of drosophila melanogaster |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1186173165/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031288561&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT iyershantanus characterizationofthenonspecificlethalnslcomplexinthegermlineofdrosophilamelanogaster |
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