B Cell Receptor Signaling: Methods and Protocols
This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell...
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
2018
|
| Schriftenreihe: | Methods in Molecular Biology
1707 |
| Schlagworte: | |
| Zusammenfassung: | This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working in the B cell field |
| Beschreibung: | This volume looks at the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently workingin the B cell field Preface…; Table of Contents…; Contributing Authors…; 1. Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution; Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, and Haiying Hang ; 2. Analyzing Mouse B-Cell Responses Specific to LCMV Infection; Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, and Lilin Ye; 3. Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors ; Zhiyong Yang and Christopher D. C. Allen ; 4. Biophysical Techniques to Study B Cell Activation: Single Molecule Imaging and Force Measurements ; Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, and Arpita Upadhyaya ; 5. DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses; Katelyn M. Spillane and Pavel Tolar; 6. - Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments; Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, and Alexander Hoffmann; 7. Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells; Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li, and Lilin Ye; 8. Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation ; Xin Gao, Lin Lin, and Di Yu ;; 9. B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy; Anurag R. Mishral and Akanksha Chaturvedi ; 10. Investigate Early Activation Events of B Cell Receptor Signaling by Membrane Associated Antigen System and Total Internal Reflection Fluorescence Microscopy; Xiaoming Bai, Xiaodong Zhao, and Chaohong Liu ; 11. Imaging the Interactions between B Cells and Antigen-Presenting Cellsal,Jia C. Wang, Madison Bolger-Munro, and Michael R. Gold; 12. - In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes; Yolanda R. Carrasco; 13. Study B Cell Antigen Receptor Nano-scale Organization by In Situ Fab Proximity Ligation Assay; Kathrin Kla¨sener, Jianying Yang, and Michael Reth; 14. Single Particle Tracking of Cell Surface Proteins; Laabiah Wasim and Bebhinn Treanor; 15. The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen; Chung Park, Il-Young Hwang, and John H. Kehrl; 16. Time-lapse Förster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells; Hae Won Sohn and Joseph Brzostowski; 17. Understanding of B Cell Receptor Signaling through a Photo-Activatable Antigen Presentation System; Jing Wang, Zhengpeng Wan, and Wanli Liur; 18. - Use of Streptolysin O-induced Membrane Damage as a Method of Studying the Function of Lipid Rafts during B Cell Activation; Heather Miller and Wenxia Song; 19. Visualization and Quantitative Analysis of the Actin Cytoskeleton upon B Cell Activation; Vid Šuštar, Marika Vainio, Pieta K. Mattila |
| Beschreibung: | Illustrationen 235 mm |
| ISBN: | 9781493974733 9781493984978 |
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| 490 | 1 | |a Methods in Molecular Biology |v 1707 | |
| 500 | |a This volume looks at the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently workingin the B cell field | ||
| 500 | |a Preface…; Table of Contents…; Contributing Authors…; 1. Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution; Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, and Haiying Hang ; 2. Analyzing Mouse B-Cell Responses Specific to LCMV Infection; Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, and Lilin Ye; 3. Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors ; Zhiyong Yang and Christopher D. C. Allen ; 4. Biophysical Techniques to Study B Cell Activation: Single Molecule Imaging and Force Measurements ; Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, and Arpita Upadhyaya ; 5. DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses; Katelyn M. Spillane and Pavel Tolar; 6. | ||
| 500 | |a - Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments; Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, and Alexander Hoffmann; 7. Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells; Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li, and Lilin Ye; 8. Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation ; Xin Gao, Lin Lin, and Di Yu ;; 9. B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy; Anurag R. Mishral and Akanksha Chaturvedi ; 10. Investigate Early Activation Events of B Cell Receptor Signaling by Membrane Associated Antigen System and Total Internal Reflection Fluorescence Microscopy; Xiaoming Bai, Xiaodong Zhao, and Chaohong Liu ; 11. Imaging the Interactions between B Cells and Antigen-Presenting Cellsal,Jia C. Wang, Madison Bolger-Munro, and Michael R. Gold; 12. | ||
| 500 | |a - In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes; Yolanda R. Carrasco; 13. Study B Cell Antigen Receptor Nano-scale Organization by In Situ Fab Proximity Ligation Assay; Kathrin Kla¨sener, Jianying Yang, and Michael Reth; 14. Single Particle Tracking of Cell Surface Proteins; Laabiah Wasim and Bebhinn Treanor; 15. The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen; Chung Park, Il-Young Hwang, and John H. Kehrl; 16. Time-lapse Förster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells; Hae Won Sohn and Joseph Brzostowski; 17. Understanding of B Cell Receptor Signaling through a Photo-Activatable Antigen Presentation System; Jing Wang, Zhengpeng Wan, and Wanli Liur; 18. | ||
| 500 | |a - Use of Streptolysin O-induced Membrane Damage as a Method of Studying the Function of Lipid Rafts during B Cell Activation; Heather Miller and Wenxia Song; 19. Visualization and Quantitative Analysis of the Actin Cytoskeleton upon B Cell Activation; Vid Šuštar, Marika Vainio, Pieta K. Mattila | ||
| 520 | |a This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working in the B cell field | ||
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| indexdate | 2024-07-10T08:20:42Z |
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| isbn | 9781493974733 9781493984978 |
| language | English |
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| physical | Illustrationen 235 mm |
| publishDate | 2018 |
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| publisher | Humana Press |
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| series | Methods in Molecular Biology |
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| spelling | B Cell Receptor Signaling Methods and Protocols edited by Chaohong Liu New York, NY Humana Press 2018 Illustrationen 235 mm txt rdacontent n rdamedia nc rdacarrier Methods in Molecular Biology 1707 This volume looks at the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently workingin the B cell field Preface…; Table of Contents…; Contributing Authors…; 1. Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution; Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, and Haiying Hang ; 2. Analyzing Mouse B-Cell Responses Specific to LCMV Infection; Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, and Lilin Ye; 3. Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors ; Zhiyong Yang and Christopher D. C. Allen ; 4. Biophysical Techniques to Study B Cell Activation: Single Molecule Imaging and Force Measurements ; Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, and Arpita Upadhyaya ; 5. DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses; Katelyn M. Spillane and Pavel Tolar; 6. - Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments; Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, and Alexander Hoffmann; 7. Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells; Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li, and Lilin Ye; 8. Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation ; Xin Gao, Lin Lin, and Di Yu ;; 9. B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy; Anurag R. Mishral and Akanksha Chaturvedi ; 10. Investigate Early Activation Events of B Cell Receptor Signaling by Membrane Associated Antigen System and Total Internal Reflection Fluorescence Microscopy; Xiaoming Bai, Xiaodong Zhao, and Chaohong Liu ; 11. Imaging the Interactions between B Cells and Antigen-Presenting Cellsal,Jia C. Wang, Madison Bolger-Munro, and Michael R. Gold; 12. - In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes; Yolanda R. Carrasco; 13. Study B Cell Antigen Receptor Nano-scale Organization by In Situ Fab Proximity Ligation Assay; Kathrin Kla¨sener, Jianying Yang, and Michael Reth; 14. Single Particle Tracking of Cell Surface Proteins; Laabiah Wasim and Bebhinn Treanor; 15. The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen; Chung Park, Il-Young Hwang, and John H. Kehrl; 16. Time-lapse Förster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells; Hae Won Sohn and Joseph Brzostowski; 17. Understanding of B Cell Receptor Signaling through a Photo-Activatable Antigen Presentation System; Jing Wang, Zhengpeng Wan, and Wanli Liur; 18. - Use of Streptolysin O-induced Membrane Damage as a Method of Studying the Function of Lipid Rafts during B Cell Activation; Heather Miller and Wenxia Song; 19. Visualization and Quantitative Analysis of the Actin Cytoskeleton upon B Cell Activation; Vid Šuštar, Marika Vainio, Pieta K. Mattila This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working in the B cell field Biochemistry Life sciences B-Lymphozyten-Rezeptor (DE-588)4326368-9 gnd rswk-swf Hardcover, Softcover / Biologie/Biochemie, Biophysik B-Lymphozyten-Rezeptor (DE-588)4326368-9 s DE-604 Liu, Chaohong Sonstige oth Erscheint auch als Online-Ausgabe 978-1-4939-7474-0 Methods in Molecular Biology 1707 (DE-604)BV035362695 1707 |
| spellingShingle | B Cell Receptor Signaling Methods and Protocols Methods in Molecular Biology Biochemistry Life sciences B-Lymphozyten-Rezeptor (DE-588)4326368-9 gnd |
| subject_GND | (DE-588)4326368-9 |
| title | B Cell Receptor Signaling Methods and Protocols |
| title_auth | B Cell Receptor Signaling Methods and Protocols |
| title_exact_search | B Cell Receptor Signaling Methods and Protocols |
| title_full | B Cell Receptor Signaling Methods and Protocols edited by Chaohong Liu |
| title_fullStr | B Cell Receptor Signaling Methods and Protocols edited by Chaohong Liu |
| title_full_unstemmed | B Cell Receptor Signaling Methods and Protocols edited by Chaohong Liu |
| title_short | B Cell Receptor Signaling |
| title_sort | b cell receptor signaling methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biochemistry Life sciences B-Lymphozyten-Rezeptor (DE-588)4326368-9 gnd |
| topic_facet | Biochemistry Life sciences B-Lymphozyten-Rezeptor |
| volume_link | (DE-604)BV035362695 |
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