Verfügbarkeit: Development basis of the heteroblastic leaf shape transition in A. thaliana

Development basis of the heteroblastic leaf shape transition in A. thaliana:

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Bibliographische Detailangaben
1. Verfasser:	Jenke, Hannah (VerfasserIn)
Format:	Abschlussarbeit Buch
Sprache:	English
Veröffentlicht:	Köln März 2018
Schlagworte:	Hochschulschrift
Online-Zugang:	Inhaltsverzeichnis Inhaltsverzeichnis
Beschreibung:	XIV, 146 Seiten Illustrationen 21 cm

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adam_text	TABLE OF CONTENTS CHAPTER 1 : INTRODUCTION...............................................................1 1.1 I NTRODUCTION TO DEVELOPMENTAL BIOLOGY .............................................. 2 1.2 T HE ORIGIN OF LEAVES : T HE SHOOT APICAL MERISTEM ............................... 3 1.2.1 HOW DOES THE WUS/CLVFEEDBACK LOOP MAINTAIN AND REGULATE THE SAM? ............................................................ 3 1.2.2 HOW DO KNOX GENES MAINTAIN THE SAM? ......................... 4 1.2.3 HOW DO HORMONES BALANCE SAM MAINTENANCE AND ORGANOGENESIS?................................................................. 7 1.2.4 THE BOUNDARY REGION BETWEEN THE SAM AND LATERAL ORGANS............................................................................... 8 1.3 L EAF MORPHOGENESIS ........................................................................................ 9 1.3.1 HOW DO LEAVES INITIATE? ..................................................... 9 1.3.2 HOW ARE THE ADAXIAL-ABAXIAL SIDES PATTERNED? ............... 11 1.3.3 HOW IS THE BLADE MARGIN PATTERNED? ............................... 12 1.3.4 THE CONTROVERSY O F LEAF MERISTEMS ................................... 15 1.4 H ETEROBLASTIC DEVELOPMENT AND THE SPL TRANSCRIPTION FACTOR FAMILY...........................................................................................................17 1.4.1 HOW IS HETEROBLASTIC DEVELOPMENT REGULATED? ................. 18 1.4.2 THE SQUAMOSA PROMOTER-BINDING PROTEIN LIKE GENE FAM ILY ........................................................... 19 1.4.3 WHAT IS THE ROLE O F SPL9 AND SPL 15 DURING THE LEAF SHAPE TRANSITION?...................................................................... 22 1.4.4 GENES, MECHANICAL FORCES ANDFORMS ............................... 23 1.5 A IMS AND OBJECTIVES ..................................................................................26 CHAPTER 2 : MATERIALS AND METHODS ..................................... 28 2.1 M OLECULAR BIOLOGY ...................................................................................... 29 2.1.1 BACTERIAL STRAINS.............................................................. 29 2.1.2 POLYMERASE CHAIN REACTION .............................................. 29 2.1.3 RNA EXTRACTION................................................................ 30 2.1.4 CONSTRUCTS AND CLONING STRATEGY ...................................... 30 2.1.5 OLIGONUCLEOTIDES............................................................. 33 2.1.6 BACTERIAL TRANSFORMATION ................................................. 34 2.1.7 PLANT TRANSFORMATION O F A. THALIANA AND C. HIRSUTA ....... 34 2.1.8 SEQUENCING REACTIONS ...................................................... 35 2.1.9 GENOMIC DNA EXTRACTION FOR POLYMERASE CHAIN REACTIONS......................................................................... 35 2.1.10 ANALYSIS O F POLYPLOIDIC NUCLEI ....................................... 35 2.2 P LANT METHODS ............................................ 36 2.2.1 PLANT GROWTH CONDITIONS .................................................. 36 2.2.2 SELECTION O F TRANSGENIC PLANTS ......................................... 36 2.2.3 PLANT MATERIAL.................................................................. 36 2.2.4 PHENOTYPIC ANALYSIS ......................................................... 38 2.2.5 CROSS-POLLINATION ............................................................. 38 2.3 M ICROSCOPY AND TIME - LAPSE EXPERIMENTS ............................................... 38 2.3.1 SCANNING ELECTRON MICROSCOPY ....................................... 38 2.3.2 CONFOCAL MICROSCOPY ....................................................... 38 2.3.3 TIME LAPSE EXPERIMENTS ................................................... 40 2.3.4 MORPHOGRAPHX........................ 40 2.3.5 STOMATA COUNTING ............................................................. 40 2.3.6 QUANTIFICATION O F DAUGHTER CELLS ...................................... 40 CHAPTER 3 : JUVENILE AND ADULT LEAF MORPHOGENESIS IN A. THALIANA AND C. HIRSUTA .............................. 42 3.1 I NTRODUCTION ....................................................................................................43 3.2 R ESULTS ...............................................................................................................45 3.2.1 ADULT A. THALIANA LEAVES GROW SLOWER IN THE MEDIAL REGION AND PETIOLE ...................................................................... 45 3.2.2 ADULT A. THALIANA LEAVES TRANSIT LATER INTO THE CELL EXPANSION PHASE............................................................. 48 3.2.3 ADULT C. HIRSUTA LEAVES EXHIBIT SLOWER GROWTH AND LATER CELL EXPANSION................................................................. 50 3.2.4 LEAVES DO NOT MAINTAIN A SPECIALIZED DOMAIN TO GENERAL BLADE AND PETIOLE CELLS .................................................... 53 3.3 D ISCUSSION ......................................................................................................... 55 3.4 C ONCLUSIONS ..................................................................................................... 59 CHAPTER 4 : SPL9 MODULATES THE PROGRESSION OF CELL DIFFERENTIATION ........................................................ 60 4.1 I NTRODUCTION ....................................................................................................61 4.2 R ESULTS ...............................................................................................................62 4.2.1 SPL9 IS HIGHLY EXPRESSED IN THE ADULT MEDIAL REGION AND PETIOLE ............................................................................. 62 4.2.2 SPL9 IS SUFFICIENT TO SWITCH JUVENILE TO ADULT GROWTH PATTERNS ........................................................................... 64 4.2.3 SPL9 MODERATES CELL EXPANSION ....................................... 66 4.2.4 SPL9 DELAYS THE DEVELOPMENT O F PAVEMENT CELLS, STOMATA AND ENDOREDUPLICATION ................................................... 68 4.3 D ISCUSSIO N ........................................................................................................ 70 4.4 C ONCLUSIONS .....................................................................................................72 CHAPTER 5 : THE EFFECT OF LOCALLY MODERATING DIFFERENTIATION ON LEAF MORPHOGENESIS. 73 5.1 I NTRODUCTION ....................................................................................................74 5.2 R E SU L T S ..............................................................................................................75 5.2.1 LOCAL SPL9R EXPRESSION CAUSES LOCAL SHAPE MODIFICATIONS NON-CELL AUTONOMOUSLY ................................................... 75 5.2.2 MARGINAL SPL9R EXPRESSION INTERFERES WITH THE MARGIN S DIFFERENTIATION ................................................................. 77 5.2.3 SPL9R EXPRESSION IN THE STIPULE AFFECTS ITS DIFFERENTIATION ................................................................. 78 5.2.4 SPL9R EXPRESSION IN THE PROXIMAL BLADE INTERFERES WITH PETIOLE-BLADE SEPARATION ................................................ 80 5.2.5 SPL9R INFLUENCES DIRECTIONAL GROWTH ............................... 81 5.3 D ISCUSSIO N ........................................................................................................ 88 5.4 C ONCLUSIONS .....................................................................................................90 CHAPTER 6 : MOLECULAR FUNCTIONS OF SPL9 ........................ 91 6.1 I NTRODUCTION ....................................................................................................92 6.2 R E SU L T S ............................................................................................................. 93 6.2.1 SPL9 MAY PROLONG CELL PROLIFERATION DOMAIN- DEPENDENTLY.................................................................... 93 6.2.2 SPL9 MAY ALTER CELL WALL PROPERTIES IN HIGHLY PROLIFERATIVE ENVIRONMENTS.................................................................. 96 6.2,3 SPL9 PROBABLY MODULATES MULTIPLE PROCESSES ............. 101 6.3 D ISCUSSION ........................................................................................................108 6.4 C ONCLUSIONS ................................................................................................... 111 CHAPTER 7 : GENERAL DISCUSSION...............................................112 7.1 SPL9 REGULATES TISSUE GROWTH THROUGH MODULATING CELL DIFFERENTIATION AND GROWTH DIRECTION..........................................113 7.2 M OLECULAR FUNCTIONS OF SPL9 ................................................................. 115 7.3 HOW IS MORPHOGENESIS CONTROLLED ? ...................................................... 118 REFERENCES........................................................................................... 121
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spelling	Jenke, Hannah Verfasser (DE-588)1164190164 aut Development basis of the heteroblastic leaf shape transition in A. thaliana vorgelegt von Hannah Jenke Köln März 2018 XIV, 146 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität zu Köln 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1169445616/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030809421&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Jenke, Hannah Development basis of the heteroblastic leaf shape transition in A. thaliana
subject_GND	(DE-588)4113937-9
title	Development basis of the heteroblastic leaf shape transition in A. thaliana
title_auth	Development basis of the heteroblastic leaf shape transition in A. thaliana
title_exact_search	Development basis of the heteroblastic leaf shape transition in A. thaliana
title_full	Development basis of the heteroblastic leaf shape transition in A. thaliana vorgelegt von Hannah Jenke
title_fullStr	Development basis of the heteroblastic leaf shape transition in A. thaliana vorgelegt von Hannah Jenke
title_full_unstemmed	Development basis of the heteroblastic leaf shape transition in A. thaliana vorgelegt von Hannah Jenke
title_short	Development basis of the heteroblastic leaf shape transition in A. thaliana
title_sort	development basis of the heteroblastic leaf shape transition in a thaliana
topic_facet	Hochschulschrift
url	http://d-nb.info/1169445616/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030809421&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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