Holdings: Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae

Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae:

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Bibliographic Details
Main Author:	Walter, Kerstin 1985- (Author)
Format:	Thesis Book
Language:	English
Published:	Aachen [2018]
Subjects:	Saccharomyces cerevisiae Biotransformation Triterpenoide Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Physical Description:	XIII, 106 Seiten Illustrationen 21 cm

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MARC


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adam_text	CONTENTS ACKNOWLEDGEMENT III SUMMARY V III ZUSAMMENFASSUNG IX LIST O F ABBREVIATIONS X GENERAL INTRODUCTION 1 1.1 SECONDARY PLANT METABOLITES 2 1.2 HETEROLOGOUS SYNTHESIS O F CYCLIC TRITERPENOIDS 4 1.3 PATHWAY ENZYMES FOR BETULINIC ACID PRODUCTION 6 1.3.1 SQUALENE SYNTHASE 6 1.3.2 SQUALENE EPOXIDASE 7 1.3.3 LUPEOL SYNTHASE 8 1.3.4 MICROSOMAL P450 SYSTEM (DASS II) 9 1.3.5 LANOSTEROL SYNTHASE 11 1.4 METABOLIE ENGINEERING STRATEGIES FOR STRAIN OPTIMIZATION 11 1.5 POLE O F ER AND LP IN TRITERPENOID PRODUCTION 13 1.6 MANIPULATION O F ER PROLIFERATION 15 1.6.1 INHIBITOR OF LIPID BIOSYNTHESIS - OPIL 17 1.6.2 CIS-PRENYLTRANSFERASE, A KEY ENZYME FOR DOLICHOL SYNTHESIS RER2 17 1.6.3 IMPACT OF PAH1 DELETION 18 1.7 SCOPE O F THIS THESIS 18 SUBCELLULAR LOCALIZATION O F HETEROLOGOUS BETULINIC ACID SYNTHESIS ENZYM ES AND INTERM EDIATES IN S. CEREVISIAE 20 2.1 SUMMARY 21 2.2 INTRODUCTION 21 2.3 MATERIALS AND METHODS 22 2.4 RESULTS 27 2.4.1 SUBCELLULAR LOCALIZATION OF TRITERPENOIDS 29 2.5 DISCUSSION 36 IM PACT O F INCREASED ENDOPLASM IC RETICULUM SIZE ON S. CEREVISIAE*S TRITERPENE AND STEROL M ETABOLISM 38 3.1 SUMMARY 39 3.2 INTRODUCTION 39 3.3 MATERIALS AND METHODS 42 3.3.2 MEDIA AND GROWTH CONDITIONS 44 3.4 RESULTS 46 3.4.1 IMPACT OF UPREGULATED MEVALONATE PATHWAY ACTIVITY ON ER SIZE 46 3.4.2 INFLUENCE OF DTT TREATMENT ON ER SIZE 47 3.4.3 ER SIZE ALTERATION IN S. CEREVISIAE AH22 RER2A 50 3.4.4 ER SIZE MORPHOLOGY ?/ //A-STRAINS 54 3.4.5 ACTIVITY OF ER-ASSOCIATED BIOSYNTHESIS OF TERPENOIDS AND STEROLS IN OPI1 A- AND W2A-STRAINS 57 3.5 DISCUSSION 60 IM PACT O F INCREASED E R SIZE ON ABUNDANCE AND ACTIVITY O F ER RESIDENT ENZYM ES 62 4.1 SUMMARY 63 4.2 INTRODUCTION 63 4.3 MATERIAL AND METHODS 65 4.4 RESULTS 68 4.1.1 IMPACT OFPAH1A ON SQUALENE PRODUCTION 68 4.1.2 CHARACTERIZATION OF TRITERPENE PRODUCTION OF STRAINS WITH ALTERED LIPID SYNTHESIS 69 4.1.3 PAH1 DELETION IN BETULINIC ACID PRODUCING IN S. CEREVISIAE CEN.PK SIMOL575 PTY-OB-BA #4 71 4.5 DISCUSSION 74 IM PACT O F ERG L DISLOCALIZATION ON CYCLIC TRITERPENOID TITER IN S. CEREVISIAE 75 5.1 SUMMARY 76 5.2 INTRODUCTION 76 5.3 MATERIAL AND M ETHODS 78 5.4 RESULTS 80 5.5 DISCUSSION 82 GENERAL D ISCU SSIO N AND O UTLOOK 83 6.1 ENZYME KINETICS O F MEM BRANE PROTEINS 84 6.2 E R ENLARGEMENT STRATEGIES 86 6.3 M EMBRANE AND CELL SURFACE ENGINEERING 87 6.4 LIPOTOXICITY EFFECTS 89 6.5 TOXICITY EFFECTS O F BETULINIC ACID 6.6 ARTIFICIAL MEMBRANES AND VESICLES 91 6.7 CONCLUSION AND OUTLOOK 94 REFERENCES 96
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spelling	Walter, Kerstin 1985- Verfasser (DE-588)1162967293 aut Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae vorgelegt von Dipl-.Ing. Kerstin Walter Aachen [2018] XIII, 106 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation RWTH Aachen 2018 Saccharomyces cerevisiae (DE-588)4178812-6 gnd rswk-swf Biotransformation (DE-588)4006908-4 gnd rswk-swf Triterpenoide (DE-588)4186237-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Biotransformation (DE-588)4006908-4 s Triterpenoide (DE-588)4186237-5 s Saccharomyces cerevisiae (DE-588)4178812-6 s DE-604 B:DE-101 application/pdf http://d-nb.info/1164997998/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030808151&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Walter, Kerstin 1985- Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae Saccharomyces cerevisiae (DE-588)4178812-6 gnd Biotransformation (DE-588)4006908-4 gnd Triterpenoide (DE-588)4186237-5 gnd
subject_GND	(DE-588)4178812-6 (DE-588)4006908-4 (DE-588)4186237-5 (DE-588)4113937-9
title	Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae
title_auth	Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae
title_exact_search	Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae
title_full	Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae vorgelegt von Dipl-.Ing. Kerstin Walter
title_fullStr	Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae vorgelegt von Dipl-.Ing. Kerstin Walter
title_full_unstemmed	Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae vorgelegt von Dipl-.Ing. Kerstin Walter
title_short	Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae
title_sort	compartment engineering to increase metabolic flux through the mevalonate pathway in s cerevisiae
topic	Saccharomyces cerevisiae (DE-588)4178812-6 gnd Biotransformation (DE-588)4006908-4 gnd Triterpenoide (DE-588)4186237-5 gnd
topic_facet	Saccharomyces cerevisiae Biotransformation Triterpenoide Hochschulschrift
url	http://d-nb.info/1164997998/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030808151&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT walterkerstin compartmentengineeringtoincreasemetabolicfluxthroughthemevalonatepathwayinscerevisiae

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