Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
[2018]
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | XIII, 106 Seiten Illustrationen 21 cm |
Internformat
MARC
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENTS
ACKNOWLEDGEMENT III
SUMMARY V III
ZUSAMMENFASSUNG IX
LIST O F ABBREVIATIONS X
GENERAL INTRODUCTION 1
1.1 SECONDARY PLANT METABOLITES 2
1.2 HETEROLOGOUS SYNTHESIS O F CYCLIC TRITERPENOIDS 4
1.3 PATHWAY ENZYMES FOR BETULINIC ACID PRODUCTION 6
1.3.1 SQUALENE SYNTHASE 6
1.3.2 SQUALENE EPOXIDASE 7
1.3.3 LUPEOL SYNTHASE 8
1.3.4 MICROSOMAL P450
SYSTEM
(DASS II) 9
1.3.5 LANOSTEROL SYNTHASE 11
1.4 METABOLIE ENGINEERING STRATEGIES FOR STRAIN OPTIMIZATION 11
1.5 POLE O F ER AND LP IN TRITERPENOID PRODUCTION 13
1.6 MANIPULATION O F ER PROLIFERATION 15
1.6.1 INHIBITOR OF LIPID BIOSYNTHESIS - OPIL 17
1.6.2 CIS-PRENYLTRANSFERASE, A KEY ENZYME FOR DOLICHOL SYNTHESIS RER2 17
1.6.3 IMPACT OF PAH1 DELETION 18
1.7 SCOPE O F THIS THESIS 18
SUBCELLULAR LOCALIZATION O F HETEROLOGOUS BETULINIC ACID SYNTHESIS ENZYM
ES AND
INTERM EDIATES IN
S. CEREVISIAE
20
2.1 SUMMARY 21
2.2 INTRODUCTION 21
2.3 MATERIALS AND METHODS 22
2.4 RESULTS 27
2.4.1 SUBCELLULAR LOCALIZATION OF TRITERPENOIDS 29
2.5 DISCUSSION 36
IM PACT O F INCREASED ENDOPLASM IC RETICULUM SIZE ON
S. CEREVISIAE*S
TRITERPENE
AND STEROL M ETABOLISM 38
3.1 SUMMARY 39
3.2 INTRODUCTION 39
3.3 MATERIALS AND METHODS 42
3.3.2 MEDIA AND GROWTH CONDITIONS 44
3.4 RESULTS 46
3.4.1 IMPACT OF UPREGULATED MEVALONATE PATHWAY ACTIVITY ON ER SIZE 46
3.4.2 INFLUENCE OF DTT TREATMENT ON ER SIZE 47
3.4.3 ER SIZE ALTERATION IN
S. CEREVISIAE AH22 RER2A 50
3.4.4 ER SIZE MORPHOLOGY ?/ //A-STRAINS 54
3.4.5 ACTIVITY OF ER-ASSOCIATED BIOSYNTHESIS OF TERPENOIDS AND STEROLS
IN
OPI1 A- AND
W2A-STRAINS 57
3.5 DISCUSSION 60
IM PACT O F INCREASED E R SIZE ON ABUNDANCE AND ACTIVITY O F ER RESIDENT
ENZYM ES 62
4.1 SUMMARY 63
4.2 INTRODUCTION 63
4.3 MATERIAL AND METHODS 65
4.4 RESULTS 68
4.1.1 IMPACT OFPAH1A ON SQUALENE PRODUCTION 68
4.1.2 CHARACTERIZATION OF TRITERPENE PRODUCTION OF STRAINS WITH ALTERED
LIPID SYNTHESIS 69
4.1.3
PAH1 DELETION IN BETULINIC ACID PRODUCING IN S. CEREVISIAE CEN.PK
SIMOL575
PTY-OB-BA #4 71
4.5 DISCUSSION 74
IM PACT O F ERG L DISLOCALIZATION ON CYCLIC TRITERPENOID TITER IN
S. CEREVISIAE
75
5.1 SUMMARY 76
5.2 INTRODUCTION 76
5.3 MATERIAL AND M ETHODS 78
5.4 RESULTS 80
5.5 DISCUSSION 82
GENERAL D ISCU SSIO N AND O UTLOOK 83
6.1 ENZYME KINETICS O F MEM BRANE PROTEINS 84
6.2 E R ENLARGEMENT STRATEGIES 86
6.3 M EMBRANE AND CELL SURFACE ENGINEERING 87
6.4 LIPOTOXICITY EFFECTS 89
6.5 TOXICITY EFFECTS O F BETULINIC ACID
6.6 ARTIFICIAL MEMBRANES AND VESICLES 91
6.7
CONCLUSION AND OUTLOOK
94
REFERENCES 96
|
| any_adam_object | 1 |
| author | Walter, Kerstin 1985- |
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| bvnumber | BV045422289 |
| ctrlnum | (OCoLC)1057452565 (DE-599)DNB1164997998 |
| discipline | Biologie |
| format | Thesis Book |
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| id | DE-604.BV045422289 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:17:43Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030808151 |
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| physical | XIII, 106 Seiten Illustrationen 21 cm |
| publishDate | 2018 |
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| spelling | Walter, Kerstin 1985- Verfasser (DE-588)1162967293 aut Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae vorgelegt von Dipl-.Ing. Kerstin Walter Aachen [2018] XIII, 106 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation RWTH Aachen 2018 Saccharomyces cerevisiae (DE-588)4178812-6 gnd rswk-swf Biotransformation (DE-588)4006908-4 gnd rswk-swf Triterpenoide (DE-588)4186237-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Biotransformation (DE-588)4006908-4 s Triterpenoide (DE-588)4186237-5 s Saccharomyces cerevisiae (DE-588)4178812-6 s DE-604 B:DE-101 application/pdf http://d-nb.info/1164997998/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030808151&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Walter, Kerstin 1985- Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae Saccharomyces cerevisiae (DE-588)4178812-6 gnd Biotransformation (DE-588)4006908-4 gnd Triterpenoide (DE-588)4186237-5 gnd |
| subject_GND | (DE-588)4178812-6 (DE-588)4006908-4 (DE-588)4186237-5 (DE-588)4113937-9 |
| title | Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae |
| title_auth | Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae |
| title_exact_search | Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae |
| title_full | Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae vorgelegt von Dipl-.Ing. Kerstin Walter |
| title_fullStr | Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae vorgelegt von Dipl-.Ing. Kerstin Walter |
| title_full_unstemmed | Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae vorgelegt von Dipl-.Ing. Kerstin Walter |
| title_short | Compartment engineering to increase metabolic flux through the mevalonate pathway in S. cerevisiae |
| title_sort | compartment engineering to increase metabolic flux through the mevalonate pathway in s cerevisiae |
| topic | Saccharomyces cerevisiae (DE-588)4178812-6 gnd Biotransformation (DE-588)4006908-4 gnd Triterpenoide (DE-588)4186237-5 gnd |
| topic_facet | Saccharomyces cerevisiae Biotransformation Triterpenoide Hochschulschrift |
| url | http://d-nb.info/1164997998/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030808151&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT walterkerstin compartmentengineeringtoincreasemetabolicfluxthroughthemevalonatepathwayinscerevisiae |
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