Microfluidics for cellular and subcellular analysis:
Saved in:
| Other Authors: | , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Cambridge, MA ; San Diego, CA ; Oxford ; London
Academic Press
2018
|
| Edition: | First edition |
| Series: | Methods in cell biology
Volume 148 Microfluidics in cell biology Part C |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xiii, 250 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128142844 |
Staff View
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Record in the Search Index
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| adam_text | Contents
Contributors........................................................ ix
Preface.............................................................xiii
SECTION I MICROFLUIDICS FOR CELL CULTURE, CELL
_______________MANIPULATION AND CELL SORTING____________________________
CHAPTER 1 Viable cell culture in PDMS-based microfluidic
devices.................................................3
Melikhan Tanyeri, Sava§ Tay
1. PDMS: Material Properties...............................4
2. Cell Density and Perfusion..............................9
3. Advantages of PDMS-Based Microfluidic Cell Culture.....11
4. Challenges of PDMS-Based Microfluidic Cell Culture.....13
5. Applications...........................................16
6. Outlook................................................24
References................................................24
CHAPTER 2 High-throughput microfluidic single-cell trapping
arrays for biomolecular and imaging analysis...........35
Xuan Li, Abraham P. Lee
1. Introduction...........................................36
2. Chip Design and Rationale............................. 37
3. Fabrication of the Microfluidic Single-Cell Trapping Array.38
4. Experimental Protocol for Single-Cell Trapping.........42
5. Application 1: Single-Cell mRNA Probing................43
6. Application 2: Single-cell Array for Live-cell Imaging.45
7. Conclusion.............................................49
Acknowledgments...........................................49
References................................................49
CHAPTER 3 Artificial niche microarrays for identifying extrinsic
cell-fate determinants.................................51
Samy Gobaa, Raphael V. Gayet, Matthias P. Lutolf
1. Introduction...........................................52
2. Method.................................................53
3. Discussion.............................................64
Acknowledgments...........................................67
References................................................68
Further Reading...........................................69
Contents
CHAPTER 4 Reconstruction of directed neuronal networks in a
microfluidic device with asymmetric
microchannels...........................................71
Josquin Courte, Renaud Renault, Audric Jan, Jean-Louis Viovy,
Jean-Michel Peyrin, Catherine Villard
1. Introduction...........................................72
2. Method.................................................74
3. Results................................................86
4. Discussion.............................................91
Acknowledgment............................................94
References................................................94
CHAPTER 5 Single-cell 3D electro-rotation.............................97
Liang Huang, Peng Zhao, Fei Liang, Wenhui Wang
1. Introduction...........................................98
2. Theory and Method.....................................100
3. Devices Design, Fabrication and Work Procedures.......102
4. Data Analysis.........................................109
5. Discussion............................................114
Acknowledgments..........................................114
References...............................................114
SECTION II DROPLET-BASED MICROFLUIDICS__________________________________
CHAPTER 6 Direct quantification of EGFR variant allele
frequency in cell-free DNA using a microfluidic-
free digital droplet PCR assay.....................119
Benjamin Demaree, Daniel Weisgerber, Ata Dolatmoradi,
Makiko Hatori, Adam R. Abate
1. Introduction..........................................120
2. Discussion............................................121
3. Methods...............................................124
4. Conclusion............................................130
References...............................................130
CHAPTER 7 Quantifying phenotypes in single cells using
droplet microfluidics..................................133
Fengjiao Lyu, Lucas R. Blauch, Sindy K.Y. Tang
1. Introduction..........................................134
2. Materials and Methods.................................136
Contents vii
3. Example 1. Quantitative Detection of Cells Expressing BlaC
Using Droplet-Based Microfluidics for Use in the Diagnosis
of Tuberculosis........................................142
4. Example 2. Measuring Variability in Metabolism With
Single-Cell Resolution.................................146
5. Conclusions............................................155
References............................................... 156
CHAPTER 8 Modular microfluidics for double emulsion
formation..............................................i6i
Bryant Thompson, Nareh Movsesian, Christine Cheng,
Prathamesh Karandikar, Malancha Gupta, Noah Malmstadt
1. Introduction...........................................162
2. Modular Fluidic and Instrumentation Components (MFICs),.. 164
3. Initiated Chemical Vapor Deposition (iCVD)....166
4. Contact Angle Measurement..............................168
5. Double Emulsion MFIC System............................169
6. Discussion.............................................171
References.............................................. 173
Further Reading...........................................175
CHAPTER 9 Universal anchored-droplet device for cellular
bioassays............................................ 177
Gabriel Amselem, Sébastien Sart, Charles N. Baroud
1. Introduction...........................................178
2. Fabrication of Hydrophobic PDMS Chips..................180
3. Producing and Manipulating Immobilized Microfluidic
Droplets...............................................185
4. Using the Microfluidic Device for Animal Cell and Bacteria
Culture and Analysis...................................190
5. Conclusions............................................196
Acknowledgments...........................................197
References................................................197
SECTION III MICROFLUIDICS FOR CELLULAR ANALYSIS
CHAPTER 10 Cell biology at the interface of nanobiosensors and
microfluidics..........................................203
Nikhil Bhalla, Hung-Ju Chiang, Amy Q. Shen
1. Introduction...........................................204
2. Nanobiosensors.........................................205
3. Microfluidic Devices...................................212
Contents
4. Nanoplasmo-Fluidics: Coupling Nanoplasmonics With
Microfluidics...........................................217
5. Challenges in Nanoplasmo-Fluidics for Cell Biology......221
6. Outlook................................... ............222
References.................................................222
CHAPTER 11 BET-seq: Binding energy topographies revealed
by microfluidics and high-throughput sequencing... 229
Arjun K, Aditham, Tyler C. Shimko, Polly M. Fordyce
1. Introduction.......................................... 230
2. BET-Seq Library Design and Synthesis....................232
3. Performing a BET-Seq Experiment.........................237
4. Sequencing Library Preparation..........................241
5. Analysis................................................245
6. Conclusion..............................................248
Acknowledgments............................................248
References............................................. 249
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| isbn | 9780128142844 |
| language | English |
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| series | Methods in cell biology Microfluidics in cell biology |
| series2 | Methods in cell biology Microfluidics in cell biology |
| spelling | Microfluidics for cellular and subcellular analysis edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel First edition Cambridge, MA ; San Diego, CA ; Oxford ; London Academic Press 2018 xiii, 250 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 148 Microfluidics in cell biology Part C Mikrofluidik (DE-588)4803438-1 gnd rswk-swf Cytologie (DE-588)4070177-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mikrofluidik (DE-588)4803438-1 s Cytologie (DE-588)4070177-3 s b DE-604 Fletcher, Daniel (DE-588)116571180X edt Doh, Junsang (DE-588)1165711648 edt Piel, Matthieu 1973- (DE-588)116571213X edt Methods in cell biology Volume 148 (DE-604)BV002534878 148 Microfluidics in cell biology Part C (DE-604)BV045262771 C Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030785783&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Microfluidics for cellular and subcellular analysis Methods in cell biology Microfluidics in cell biology Mikrofluidik (DE-588)4803438-1 gnd Cytologie (DE-588)4070177-3 gnd |
| subject_GND | (DE-588)4803438-1 (DE-588)4070177-3 (DE-588)4143413-4 |
| title | Microfluidics for cellular and subcellular analysis |
| title_auth | Microfluidics for cellular and subcellular analysis |
| title_exact_search | Microfluidics for cellular and subcellular analysis |
| title_full | Microfluidics for cellular and subcellular analysis edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel |
| title_fullStr | Microfluidics for cellular and subcellular analysis edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel |
| title_full_unstemmed | Microfluidics for cellular and subcellular analysis edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel |
| title_short | Microfluidics for cellular and subcellular analysis |
| title_sort | microfluidics for cellular and subcellular analysis |
| topic | Mikrofluidik (DE-588)4803438-1 gnd Cytologie (DE-588)4070177-3 gnd |
| topic_facet | Mikrofluidik Cytologie Aufsatzsammlung |
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