Microfluidics for cellular and subcellular analysis:
Gespeichert in:
| Weitere Verfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA ; San Diego, CA ; Oxford ; London
Academic Press
2018
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in cell biology
Volume 148 Microfluidics in cell biology Part C |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xiii, 250 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128142844 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV045399665 | ||
| 003 | DE-604 | ||
| 005 | 20190201 | ||
| 007 | t | ||
| 008 | 190115s2018 a||| |||| 00||| eng d | ||
| 020 | |a 9780128142844 |9 978-0-12-814284-4 | ||
| 035 | |a (OCoLC)1083283030 | ||
| 035 | |a (DE-599)BVBBV045399665 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 084 | |a WC 5100 |0 (DE-625)148108:13423 |2 rvk | ||
| 245 | 1 | 0 | |a Microfluidics for cellular and subcellular analysis |c edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel |
| 250 | |a First edition | ||
| 264 | 1 | |a Cambridge, MA ; San Diego, CA ; Oxford ; London |b Academic Press |c 2018 | |
| 300 | |a xiii, 250 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v Volume 148 | |
| 490 | 1 | |a Microfluidics in cell biology |v Part C | |
| 650 | 0 | 7 | |a Mikrofluidik |0 (DE-588)4803438-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Cytologie |0 (DE-588)4070177-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Mikrofluidik |0 (DE-588)4803438-1 |D s |
| 689 | 0 | 1 | |a Cytologie |0 (DE-588)4070177-3 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Fletcher, Daniel |0 (DE-588)116571180X |4 edt | |
| 700 | 1 | |a Doh, Junsang |0 (DE-588)1165711648 |4 edt | |
| 700 | 1 | |a Piel, Matthieu |d 1973- |0 (DE-588)116571213X |4 edt | |
| 830 | 0 | |a Methods in cell biology |v Volume 148 |w (DE-604)BV002534878 |9 148 | |
| 830 | 0 | |a Microfluidics in cell biology |v Part C |w (DE-604)BV045262771 |9 C | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg - ADAM Catalogue Enrichment |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030785783&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030785783 | ||
Datensatz im Suchindex
| _version_ | 1804179267126296576 |
|---|---|
| adam_text | Contents
Contributors........................................................ ix
Preface.............................................................xiii
SECTION I MICROFLUIDICS FOR CELL CULTURE, CELL
_______________MANIPULATION AND CELL SORTING____________________________
CHAPTER 1 Viable cell culture in PDMS-based microfluidic
devices.................................................3
Melikhan Tanyeri, Sava§ Tay
1. PDMS: Material Properties...............................4
2. Cell Density and Perfusion..............................9
3. Advantages of PDMS-Based Microfluidic Cell Culture.....11
4. Challenges of PDMS-Based Microfluidic Cell Culture.....13
5. Applications...........................................16
6. Outlook................................................24
References................................................24
CHAPTER 2 High-throughput microfluidic single-cell trapping
arrays for biomolecular and imaging analysis...........35
Xuan Li, Abraham P. Lee
1. Introduction...........................................36
2. Chip Design and Rationale............................. 37
3. Fabrication of the Microfluidic Single-Cell Trapping Array.38
4. Experimental Protocol for Single-Cell Trapping.........42
5. Application 1: Single-Cell mRNA Probing................43
6. Application 2: Single-cell Array for Live-cell Imaging.45
7. Conclusion.............................................49
Acknowledgments...........................................49
References................................................49
CHAPTER 3 Artificial niche microarrays for identifying extrinsic
cell-fate determinants.................................51
Samy Gobaa, Raphael V. Gayet, Matthias P. Lutolf
1. Introduction...........................................52
2. Method.................................................53
3. Discussion.............................................64
Acknowledgments...........................................67
References................................................68
Further Reading...........................................69
Contents
CHAPTER 4 Reconstruction of directed neuronal networks in a
microfluidic device with asymmetric
microchannels...........................................71
Josquin Courte, Renaud Renault, Audric Jan, Jean-Louis Viovy,
Jean-Michel Peyrin, Catherine Villard
1. Introduction...........................................72
2. Method.................................................74
3. Results................................................86
4. Discussion.............................................91
Acknowledgment............................................94
References................................................94
CHAPTER 5 Single-cell 3D electro-rotation.............................97
Liang Huang, Peng Zhao, Fei Liang, Wenhui Wang
1. Introduction...........................................98
2. Theory and Method.....................................100
3. Devices Design, Fabrication and Work Procedures.......102
4. Data Analysis.........................................109
5. Discussion............................................114
Acknowledgments..........................................114
References...............................................114
SECTION II DROPLET-BASED MICROFLUIDICS__________________________________
CHAPTER 6 Direct quantification of EGFR variant allele
frequency in cell-free DNA using a microfluidic-
free digital droplet PCR assay.....................119
Benjamin Demaree, Daniel Weisgerber, Ata Dolatmoradi,
Makiko Hatori, Adam R. Abate
1. Introduction..........................................120
2. Discussion............................................121
3. Methods...............................................124
4. Conclusion............................................130
References...............................................130
CHAPTER 7 Quantifying phenotypes in single cells using
droplet microfluidics..................................133
Fengjiao Lyu, Lucas R. Blauch, Sindy K.Y. Tang
1. Introduction..........................................134
2. Materials and Methods.................................136
Contents vii
3. Example 1. Quantitative Detection of Cells Expressing BlaC
Using Droplet-Based Microfluidics for Use in the Diagnosis
of Tuberculosis........................................142
4. Example 2. Measuring Variability in Metabolism With
Single-Cell Resolution.................................146
5. Conclusions............................................155
References............................................... 156
CHAPTER 8 Modular microfluidics for double emulsion
formation..............................................i6i
Bryant Thompson, Nareh Movsesian, Christine Cheng,
Prathamesh Karandikar, Malancha Gupta, Noah Malmstadt
1. Introduction...........................................162
2. Modular Fluidic and Instrumentation Components (MFICs),.. 164
3. Initiated Chemical Vapor Deposition (iCVD)....166
4. Contact Angle Measurement..............................168
5. Double Emulsion MFIC System............................169
6. Discussion.............................................171
References.............................................. 173
Further Reading...........................................175
CHAPTER 9 Universal anchored-droplet device for cellular
bioassays............................................ 177
Gabriel Amselem, Sébastien Sart, Charles N. Baroud
1. Introduction...........................................178
2. Fabrication of Hydrophobic PDMS Chips..................180
3. Producing and Manipulating Immobilized Microfluidic
Droplets...............................................185
4. Using the Microfluidic Device for Animal Cell and Bacteria
Culture and Analysis...................................190
5. Conclusions............................................196
Acknowledgments...........................................197
References................................................197
SECTION III MICROFLUIDICS FOR CELLULAR ANALYSIS
CHAPTER 10 Cell biology at the interface of nanobiosensors and
microfluidics..........................................203
Nikhil Bhalla, Hung-Ju Chiang, Amy Q. Shen
1. Introduction...........................................204
2. Nanobiosensors.........................................205
3. Microfluidic Devices...................................212
Contents
4. Nanoplasmo-Fluidics: Coupling Nanoplasmonics With
Microfluidics...........................................217
5. Challenges in Nanoplasmo-Fluidics for Cell Biology......221
6. Outlook................................... ............222
References.................................................222
CHAPTER 11 BET-seq: Binding energy topographies revealed
by microfluidics and high-throughput sequencing... 229
Arjun K, Aditham, Tyler C. Shimko, Polly M. Fordyce
1. Introduction.......................................... 230
2. BET-Seq Library Design and Synthesis....................232
3. Performing a BET-Seq Experiment.........................237
4. Sequencing Library Preparation..........................241
5. Analysis................................................245
6. Conclusion..............................................248
Acknowledgments............................................248
References............................................. 249
|
| any_adam_object | 1 |
| author2 | Fletcher, Daniel Doh, Junsang Piel, Matthieu 1973- |
| author2_role | edt edt edt |
| author2_variant | d f df j d jd m p mp |
| author_GND | (DE-588)116571180X (DE-588)1165711648 (DE-588)116571213X |
| author_facet | Fletcher, Daniel Doh, Junsang Piel, Matthieu 1973- |
| building | Verbundindex |
| bvnumber | BV045399665 |
| classification_rvk | WC 5100 |
| ctrlnum | (OCoLC)1083283030 (DE-599)BVBBV045399665 |
| discipline | Biologie |
| edition | First edition |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01893nam a2200433 cb4500</leader><controlfield tag="001">BV045399665</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20190201 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">190115s2018 a||| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780128142844</subfield><subfield code="9">978-0-12-814284-4</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1083283030</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV045399665</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 5100</subfield><subfield code="0">(DE-625)148108:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Microfluidics for cellular and subcellular analysis</subfield><subfield code="c">edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">First edition</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Cambridge, MA ; San Diego, CA ; Oxford ; London</subfield><subfield code="b">Academic Press</subfield><subfield code="c">2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">xiii, 250 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in cell biology</subfield><subfield code="v">Volume 148</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Microfluidics in cell biology</subfield><subfield code="v">Part C</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mikrofluidik</subfield><subfield code="0">(DE-588)4803438-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Cytologie</subfield><subfield code="0">(DE-588)4070177-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Mikrofluidik</subfield><subfield code="0">(DE-588)4803438-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Cytologie</subfield><subfield code="0">(DE-588)4070177-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fletcher, Daniel</subfield><subfield code="0">(DE-588)116571180X</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Doh, Junsang</subfield><subfield code="0">(DE-588)1165711648</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Piel, Matthieu</subfield><subfield code="d">1973-</subfield><subfield code="0">(DE-588)116571213X</subfield><subfield code="4">edt</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in cell biology</subfield><subfield code="v">Volume 148</subfield><subfield code="w">(DE-604)BV002534878</subfield><subfield code="9">148</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Microfluidics in cell biology</subfield><subfield code="v">Part C</subfield><subfield code="w">(DE-604)BV045262771</subfield><subfield code="9">C</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg - ADAM Catalogue Enrichment</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030785783&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030785783</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV045399665 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:17:09Z |
| institution | BVB |
| isbn | 9780128142844 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030785783 |
| oclc_num | 1083283030 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | xiii, 250 Seiten Illustrationen, Diagramme |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| publisher | Academic Press |
| record_format | marc |
| series | Methods in cell biology Microfluidics in cell biology |
| series2 | Methods in cell biology Microfluidics in cell biology |
| spelling | Microfluidics for cellular and subcellular analysis edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel First edition Cambridge, MA ; San Diego, CA ; Oxford ; London Academic Press 2018 xiii, 250 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 148 Microfluidics in cell biology Part C Mikrofluidik (DE-588)4803438-1 gnd rswk-swf Cytologie (DE-588)4070177-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mikrofluidik (DE-588)4803438-1 s Cytologie (DE-588)4070177-3 s b DE-604 Fletcher, Daniel (DE-588)116571180X edt Doh, Junsang (DE-588)1165711648 edt Piel, Matthieu 1973- (DE-588)116571213X edt Methods in cell biology Volume 148 (DE-604)BV002534878 148 Microfluidics in cell biology Part C (DE-604)BV045262771 C Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030785783&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Microfluidics for cellular and subcellular analysis Methods in cell biology Microfluidics in cell biology Mikrofluidik (DE-588)4803438-1 gnd Cytologie (DE-588)4070177-3 gnd |
| subject_GND | (DE-588)4803438-1 (DE-588)4070177-3 (DE-588)4143413-4 |
| title | Microfluidics for cellular and subcellular analysis |
| title_auth | Microfluidics for cellular and subcellular analysis |
| title_exact_search | Microfluidics for cellular and subcellular analysis |
| title_full | Microfluidics for cellular and subcellular analysis edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel |
| title_fullStr | Microfluidics for cellular and subcellular analysis edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel |
| title_full_unstemmed | Microfluidics for cellular and subcellular analysis edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel |
| title_short | Microfluidics for cellular and subcellular analysis |
| title_sort | microfluidics for cellular and subcellular analysis |
| topic | Mikrofluidik (DE-588)4803438-1 gnd Cytologie (DE-588)4070177-3 gnd |
| topic_facet | Mikrofluidik Cytologie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030785783&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 (DE-604)BV045262771 |
| work_keys_str_mv | AT fletcherdaniel microfluidicsforcellularandsubcellularanalysis AT dohjunsang microfluidicsforcellularandsubcellularanalysis AT pielmatthieu microfluidicsforcellularandsubcellularanalysis |