Holdings: Mechanical control of lateral roots development in Arabidopsis thaliana

Mechanical control of lateral roots development in Arabidopsis thaliana:

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Bibliographic Details
Main Author:	Lasok, Hanna (Author)
Format:	Thesis Book
Language:	English
Published:	Freiburg im Breisgau 2018
Subjects:	Ackerschmalwand Mechanischer Reiz Stressreaktion Wachstum Seitenwurzel Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Physical Description:	168 Seiten Illustrationen, Diagramme 30 cm

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MARC


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adam_text	IV. TABLE OF CONTENTS I. ABSTRACT IN ENGLISH......................................................................................................................... 3 II. ZUSAMMENFASSUNG AUF DEUTSCH.................................................................................................. 4 III. ACKNOWLEDGMENT............................................................................................................................ 5 IV. TABLE OF CONTENTS......................................... 6 V. FIGURE INDEX...................................................................................................................................10 VI. TABLE INDEX....................................................................................................................................11 VII. ABBREVIATIONS................................................................................................................................ 12 1 INTRODUCTION...................................................................................................................................16 1.1 PLANT ADAPTATION TO THE ENVIRONMENT................................................................................ 16 1.2 PLANT ROOT SYSTEM ARCHITECTURE...........................................................................................17 1.3 THE ARCHITECTURE OF THE PRIMARY ROOT ................................................................................ 18 1.3.1 THE ROOT APICAL MERISTEM (RAM)................................................................................18 1.4 AUXIN IS A KEY REGULATOR OF PLANT DEVELOPMENT.............................................................. 20 1.4.1 POLAR AUXIN TRANSPORT (PAT).........................................................................................21 1.4.2 AUXIN SIGNALING.............................................................................................................. 22 1.5 LATERAL ROOT DEVELOPMENT IN ARABIDOPSIS THALIANA ........................................................ 24 1.5.1 LR PRE-INITIATION............................................................................................................. 25 1.5.2 LR INITIATION.................................................................................................................... 27 1.5.3 LR PATTERNING..................................................................................................................28 1.5.4 LR EMERGENCE (OVERGROWTH)....................................................................................... 31 1.5.5 LATERAL ROOT ELONGATION..................................................................................................34 1.6 REGULATION OF LR FORMATION BY THE CROSSTALK BETWEEN AUXIN AND OTHER PLANT HORMONES .................................................................................................................................................35 1.7 THE IMPACT OF ABIOTIC FACTORS ON ROOT AND LR DEVELOPMENT ........................................ 37 1.7.1 GRAVITY RESPONSE AND ITS IMPACT ON LR DEVELOPMENT............................................37 1.7.2 THE IMPACT OF WATER ON ROOT GROWTH..........................................................................38 1.7.3 REGULATION OF ROOTS DEVELOPMENT BY NUTRIENTS ........................................................ 40 1.8 THE IMPORTANCE OF MECHANICAL FORCES IN PLANT DEVELOPMENT......................................46 1.8.1 THE PERCEPTION AND SIGNAL TRANSDUCTION OF MECHANICAL FORCES .............................. 47 1.8.2 THE IMPACT OF MECHANICAL FORCES ON PRIMARY ROOT GROWTH......................................50 1.8.3 THE IMPACT OF MECHANICAL FORCES ON LR DEVELOPMENT...........................................52 2 AIM OF PHD.................................................................................................................................... 53 3 RESULTS........................................................................................................................................... 54 3.1 ISOLATION AND IDENTIFICATION OF EMS57, A MUTANT UNABLE TO PRODUCE LATERAL ROOTS IN RESPONSE TO MECHANICAL STIMULUS ..................... ........................................................................... 54 3.2 CHARACTERIZATION OF EMS57..................................................................................................63 3.2.1 THE IMPACT OF MECHANICAL FRICTION ON EMS57 ROOT GROWTH......................................63 3.2.2 THE IMPACT OF AGAR CONCENTRATION ON LATERAL ROOT DEVELOPMENT...........................66 3.2.3 THE IMPACT OF PLATES TILTING ON EMS57 ROOT DEVELOPMENT ...................................... 69 3.2.4 THE IMPACT OF SHAKING PLATES ON EMS57 ROOT DEVELOPMENT..................................72 3.3 TRANSCRIPTOMIC ANALYSIS REVEALED THE IMPACT OF THE MUTATION ON GENE EXPRESSION PATTERN IN NONE-STIMULATED AND MECHANICALLY STIMULATED PLANTS.............................................74 3.3.1 TRANSCRIPTOMIC ANALYSIS OF NONE-SHOOK MUTANT REVEALED THAT THE EMS57-GENE REPRESS ACTIVATION OF PLANT RESPONSE TO EXTERNAL STIMULI ....................................................... 75 3.3.2 TRANSCRIPTOMIC ANALYSIS OF SHOOK PLANTS: ANALYSIS OF GENES INDUCED BY MECHANICAL STIMULUS IN THE WT AND THE MUTANT..................................................................... 78 3.3.3 GENES DIFFERENTIALLY REGULATED OVER TIME IN SHOOK W T AND THE MUTANT .............. 84 3.3.4 CLASSIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN MECHANICALLY STIMULATED W T AND MUTANT INTO FUNCTIONAL CATEGORIES.....................................................................................85 3.3.5 GENES DIFFERENTIALLY REGULATED IN THE MUTANT VERSUS WT 1,5H, 4H, AND 6H AFTER SHAKING ........................................................................................................................................87 3.4 IMPACT OF VARIOUS NUTRIENTS ON EMS57 GROWTH AND DEVELOPMENT ............................... 91 3.4.1 IMPACT OF IRON AND SULFUR NUTRITION ON PLANT DEVELOPMENT......................................91 3.4.2 IMPACT OF NITROGEN NUTRITION ON PLANT DEVELOPMENT ................................................. 92 3.4.3 IMPACT OF PHOSPHATE NUTRITION ON PLANT DEVELOPMENT.............................................94 3.4.4 CROSSTALK BETWEEN PHOSPHATE AND MECHANICAL STIMULUS ..................................... 102 4 DISCUSSION...................................................................................................................................105 4.1.1 COMPARISON OF NONE-STIMULATED MUTANT TO NONE-STIMULATED W T ...................... 107 4.1.2 COMPARISON OF MUTANT AND WT RESPONSE TO SHAKING ........................................... 108 4.2 CHARACTERIZATION OF THE GENE PERTURBED IN EMS57 MUTANT............................................112 4.3 CONCLUSIONS AND OUTLOOK....................................................................................................113 5 MATERIAL AND METHODS................................................................................................................114 5.1 MATERIALS.............................................................................................................................. 114 5.1.1 PLANT LINES....................................................................................................................114 5.1.2 ACCESSION NUMBERS....................................................................................................114 5.1.3 OLIGONUCLEOTIDES................................................................................................. 114 5.1.4 MOLECULAR BIOLOGY MATERIAL........................................................................................ 119 5.1.5 CHEMICALS....................................................................................................................119 5.1.6 OTHER MATERIALS............................................................................................................121 5.1.7 DEVICES........................................................................................................................ 121 5.1.8 PROGRAMS + WEBSITES................................................................................................. 122 5.1.9 PLANT MEDIA................................................................................................................. 123 5.1.10 SOIL................................................................................................................................ 126 5.1.11 BUFFERS AND SOLUTIONS................................................................................................. 126 5.2 METHODS............................................................................................................................... 127 5.2.1 ARABIDOPSIS THALIANA SEED STERILIZATION ................................................................... 127 5.2.2 PLANT GROWTH CONDITIONS.............................................................................................128 5.2.3 THE SELECTION OF HOMOZYGOTES IN MAP-BASED CLONING...........................................128 5.2.4 EXTRACTION OF PLANT D N A .............................................................................................128 5.2.5 RNA EXTRACTION FROM PLANTS.......................................................................................129 5.2.6 P C R ..............................................................................................................................129 5.2.7 ELECTROPHORESIS.......................................................................................................... 129 5.2.6 RT-QPC R ......................................................................................................................129 5.2.9 PHOSPHATE MEASUREMENTS........................................................................................129 5.2.10 GUS STAINING OF ARABIDOPSIS THALIANA .................................................................... 130 5.2.11 NBT STAINING OF ARABIDOPSIS THALIANA.......................................................................130 5.2.12 FM4-64 STAINING OF ARABIDOPSIS THALIANA................................................................130 5.2.13 ROOT LENGTH MEASUREMENTS......................................................................................130 5.2.14 MEASUREMENT OF ROOT HAIR DENSITY AND THE DISTANCE BETWEEN QC AND FIRST ROOT HAIR ....................................................................................................................................... 130 5.2.15 COUNT OF LATERAL ROOTS.................................................................................................131 5.2.16 CHLOROPHYLL MEASUREMENTS ...................................................................................... 131 5.2.17 EMS MUTAGENESIS OF ARABIDOPSIS THALIANA SEEDS................................................131 5.2.18 IDENTIFICATION OF EMS MUTANT IMPAIRED IN LR PRODUCTION BY MECHANICAL STIMULUS ............................................................................................................................................. . 131 5.2.19 MICROSCOPY..................................................................................................................131 5.2.20 TRANSCRIPTOME ANALYSIS............................................................................................ 131 6 REFERENCES ...................... 135 7 SUPPLEMENTARY DATA..................................................................................................................152 7.1 SUPPLEMENTARY TABLE. GO SIGNIFICANTLY OVERREPRESENTED IN W T AFTER SHAKING ..... 152 7.2 SUPPLEMENTARY TABLE. GO SIGNIFICANTLY OVERREPRESENTED IN WT OR MUTANT AFTER SHAKING........................................................................................................................................... 160
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author	Lasok, Hanna
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spellingShingle	Lasok, Hanna Mechanical control of lateral roots development in Arabidopsis thaliana Ackerschmalwand (DE-588)4141299-0 gnd Mechanischer Reiz (DE-588)4447802-1 gnd Stressreaktion (DE-588)4138565-2 gnd Wachstum (DE-588)4064115-6 gnd Seitenwurzel (DE-588)4180779-0 gnd
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title	Mechanical control of lateral roots development in Arabidopsis thaliana
title_auth	Mechanical control of lateral roots development in Arabidopsis thaliana
title_exact_search	Mechanical control of lateral roots development in Arabidopsis thaliana
title_full	Mechanical control of lateral roots development in Arabidopsis thaliana vorgelegt von Hanna Lasok
title_fullStr	Mechanical control of lateral roots development in Arabidopsis thaliana vorgelegt von Hanna Lasok
title_full_unstemmed	Mechanical control of lateral roots development in Arabidopsis thaliana vorgelegt von Hanna Lasok
title_short	Mechanical control of lateral roots development in Arabidopsis thaliana
title_sort	mechanical control of lateral roots development in arabidopsis thaliana
topic	Ackerschmalwand (DE-588)4141299-0 gnd Mechanischer Reiz (DE-588)4447802-1 gnd Stressreaktion (DE-588)4138565-2 gnd Wachstum (DE-588)4064115-6 gnd Seitenwurzel (DE-588)4180779-0 gnd
topic_facet	Ackerschmalwand Mechanischer Reiz Stressreaktion Wachstum Seitenwurzel Hochschulschrift
url	http://d-nb.info/1170282113/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030763814&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT lasokhanna mechanicalcontroloflateralrootsdevelopmentinarabidopsisthaliana

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