Mechanical control of lateral roots development in Arabidopsis thaliana:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Freiburg im Breisgau
2018
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 168 Seiten Illustrationen, Diagramme 30 cm |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | IV. TABLE OF CONTENTS
I. ABSTRACT IN
ENGLISH.........................................................................................................................
3
II. ZUSAMMENFASSUNG AUF
DEUTSCH..................................................................................................
4
III.
ACKNOWLEDGMENT............................................................................................................................
5
IV. TABLE OF CONTENTS......................................... 6
V. FIGURE
INDEX...................................................................................................................................10
VI. TABLE
INDEX....................................................................................................................................11
VII.
ABBREVIATIONS................................................................................................................................
12
1
INTRODUCTION...................................................................................................................................16
1.1 PLANT ADAPTATION TO THE
ENVIRONMENT................................................................................
16
1.2 PLANT ROOT SYSTEM
ARCHITECTURE...........................................................................................17
1.3 THE ARCHITECTURE OF THE PRIMARY ROOT
................................................................................
18
1.3.1 THE ROOT APICAL MERISTEM
(RAM)................................................................................18
1.4 AUXIN IS A KEY REGULATOR OF PLANT
DEVELOPMENT..............................................................
20
1.4.1 POLAR AUXIN TRANSPORT
(PAT).........................................................................................21
1.4.2 AUXIN
SIGNALING..............................................................................................................
22
1.5 LATERAL ROOT DEVELOPMENT IN ARABIDOPSIS THALIANA
........................................................
24
1.5.1 LR
PRE-INITIATION.............................................................................................................
25
1.5.2 LR
INITIATION....................................................................................................................
27
1.5.3 LR
PATTERNING..................................................................................................................28
1.5.4 LR EMERGENCE
(OVERGROWTH).......................................................................................
31
1.5.5 LATERAL ROOT
ELONGATION..................................................................................................34
1.6 REGULATION OF LR FORMATION BY THE CROSSTALK BETWEEN AUXIN AND OTHER
PLANT HORMONES
.................................................................................................................................................35
1.7 THE IMPACT OF ABIOTIC FACTORS ON ROOT AND LR DEVELOPMENT
........................................
37
1.7.1 GRAVITY RESPONSE AND ITS IMPACT ON LR
DEVELOPMENT............................................37
1.7.2 THE IMPACT OF WATER ON ROOT
GROWTH..........................................................................38
1.7.3 REGULATION OF ROOTS DEVELOPMENT BY NUTRIENTS
........................................................
40
1.8 THE IMPORTANCE OF MECHANICAL FORCES IN PLANT
DEVELOPMENT......................................46
1.8.1 THE PERCEPTION AND SIGNAL TRANSDUCTION OF MECHANICAL FORCES
..............................
47
1.8.2 THE IMPACT OF MECHANICAL FORCES ON PRIMARY ROOT
GROWTH......................................50
1.8.3 THE IMPACT OF MECHANICAL FORCES ON LR
DEVELOPMENT...........................................52
2 AIM OF
PHD....................................................................................................................................
53
3
RESULTS...........................................................................................................................................
54
3.1 ISOLATION AND IDENTIFICATION OF EMS57, A MUTANT UNABLE TO PRODUCE
LATERAL ROOTS IN
RESPONSE TO MECHANICAL STIMULUS
.....................
...........................................................................
54
3.2 CHARACTERIZATION OF
EMS57..................................................................................................63
3.2.1 THE IMPACT OF MECHANICAL FRICTION ON EMS57 ROOT
GROWTH......................................63
3.2.2 THE IMPACT OF AGAR CONCENTRATION ON LATERAL ROOT
DEVELOPMENT...........................66
3.2.3 THE IMPACT OF PLATES TILTING ON EMS57 ROOT DEVELOPMENT
......................................
69
3.2.4 THE IMPACT OF SHAKING PLATES ON EMS57 ROOT
DEVELOPMENT..................................72
3.3 TRANSCRIPTOMIC ANALYSIS REVEALED THE IMPACT OF THE MUTATION ON GENE
EXPRESSION
PATTERN IN NONE-STIMULATED AND MECHANICALLY STIMULATED
PLANTS.............................................74
3.3.1 TRANSCRIPTOMIC ANALYSIS OF NONE-SHOOK MUTANT REVEALED THAT THE
EMS57-GENE
REPRESS ACTIVATION OF PLANT RESPONSE TO EXTERNAL STIMULI
.......................................................
75
3.3.2 TRANSCRIPTOMIC ANALYSIS OF SHOOK PLANTS: ANALYSIS OF GENES INDUCED
BY
MECHANICAL STIMULUS IN THE WT AND THE
MUTANT.....................................................................
78
3.3.3 GENES DIFFERENTIALLY REGULATED OVER TIME IN SHOOK W T AND THE
MUTANT
..............
84
3.3.4 CLASSIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN MECHANICALLY
STIMULATED W T
AND MUTANT INTO FUNCTIONAL
CATEGORIES.....................................................................................85
3.3.5 GENES DIFFERENTIALLY REGULATED IN THE MUTANT VERSUS WT 1,5H, 4H,
AND 6H AFTER
SHAKING
........................................................................................................................................87
3.4 IMPACT OF VARIOUS NUTRIENTS ON EMS57 GROWTH AND DEVELOPMENT
...............................
91
3.4.1 IMPACT OF IRON AND SULFUR NUTRITION ON PLANT
DEVELOPMENT......................................91
3.4.2 IMPACT OF NITROGEN NUTRITION ON PLANT DEVELOPMENT
.................................................
92
3.4.3 IMPACT OF PHOSPHATE NUTRITION ON PLANT
DEVELOPMENT.............................................94
3.4.4 CROSSTALK BETWEEN PHOSPHATE AND MECHANICAL STIMULUS
.....................................
102
4
DISCUSSION...................................................................................................................................105
4.1.1 COMPARISON OF NONE-STIMULATED MUTANT TO NONE-STIMULATED W T
......................
107
4.1.2 COMPARISON OF MUTANT AND WT RESPONSE TO SHAKING
...........................................
108
4.2 CHARACTERIZATION OF THE GENE PERTURBED IN EMS57
MUTANT............................................112
4.3 CONCLUSIONS AND
OUTLOOK....................................................................................................113
5 MATERIAL AND
METHODS................................................................................................................114
5.1
MATERIALS..............................................................................................................................
114
5.1.1 PLANT
LINES....................................................................................................................114
5.1.2 ACCESSION
NUMBERS....................................................................................................114
5.1.3
OLIGONUCLEOTIDES.................................................................................................
114
5.1.4 MOLECULAR BIOLOGY
MATERIAL........................................................................................
119
5.1.5
CHEMICALS....................................................................................................................119
5.1.6 OTHER
MATERIALS............................................................................................................121
5.1.7
DEVICES........................................................................................................................
121
5.1.8 PROGRAMS +
WEBSITES.................................................................................................
122
5.1.9 PLANT
MEDIA.................................................................................................................
123
5.1.10
SOIL................................................................................................................................
126
5.1.11 BUFFERS AND
SOLUTIONS.................................................................................................
126
5.2
METHODS...............................................................................................................................
127
5.2.1 ARABIDOPSIS THALIANA SEED STERILIZATION
...................................................................
127
5.2.2 PLANT GROWTH
CONDITIONS.............................................................................................128
5.2.3 THE SELECTION OF HOMOZYGOTES IN MAP-BASED
CLONING...........................................128
5.2.4 EXTRACTION OF PLANT D N A
.............................................................................................128
5.2.5 RNA EXTRACTION FROM
PLANTS.......................................................................................129
5.2.6 P C R
..............................................................................................................................129
5.2.7
ELECTROPHORESIS..........................................................................................................
129
5.2.6 RT-QPC R
......................................................................................................................129
5.2.9 PHOSPHATE
MEASUREMENTS........................................................................................129
5.2.10 GUS STAINING OF ARABIDOPSIS THALIANA
....................................................................
130
5.2.11 NBT STAINING OF ARABIDOPSIS
THALIANA.......................................................................130
5.2.12 FM4-64 STAINING OF ARABIDOPSIS
THALIANA................................................................130
5.2.13 ROOT LENGTH
MEASUREMENTS......................................................................................130
5.2.14 MEASUREMENT OF ROOT HAIR DENSITY AND THE DISTANCE BETWEEN QC AND
FIRST ROOT HAIR
.......................................................................................................................................
130
5.2.15 COUNT OF LATERAL
ROOTS.................................................................................................131
5.2.16 CHLOROPHYLL MEASUREMENTS
......................................................................................
131
5.2.17 EMS MUTAGENESIS OF ARABIDOPSIS THALIANA
SEEDS................................................131
5.2.18 IDENTIFICATION OF EMS MUTANT IMPAIRED IN LR PRODUCTION BY
MECHANICAL STIMULUS
.............................................................................................................................................
.
131
5.2.19
MICROSCOPY..................................................................................................................131
5.2.20 TRANSCRIPTOME
ANALYSIS............................................................................................
131
6 REFERENCES
......................
135
7 SUPPLEMENTARY
DATA..................................................................................................................152
7.1 SUPPLEMENTARY TABLE. GO SIGNIFICANTLY OVERREPRESENTED IN W T AFTER
SHAKING
.....
152
7.2 SUPPLEMENTARY TABLE. GO SIGNIFICANTLY OVERREPRESENTED IN WT OR
MUTANT AFTER
SHAKING...........................................................................................................................................
160
|
| any_adam_object | 1 |
| author | Lasok, Hanna |
| author_GND | (DE-588)1164187708 |
| author_facet | Lasok, Hanna |
| author_role | aut |
| author_sort | Lasok, Hanna |
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| building | Verbundindex |
| bvnumber | BV045377419 |
| ctrlnum | (OCoLC)1090101911 (DE-599)BSZ508421055 |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Lasok, Hanna Verfasser (DE-588)1164187708 aut Mechanical control of lateral roots development in Arabidopsis thaliana vorgelegt von Hanna Lasok Freiburg im Breisgau 2018 168 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Albert-Ludwigs-Universität Freiburg im Breisgau 2018 Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Mechanischer Reiz (DE-588)4447802-1 gnd rswk-swf Stressreaktion (DE-588)4138565-2 gnd rswk-swf Wachstum (DE-588)4064115-6 gnd rswk-swf Seitenwurzel (DE-588)4180779-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Wachstum (DE-588)4064115-6 s Seitenwurzel (DE-588)4180779-0 s Mechanischer Reiz (DE-588)4447802-1 s Stressreaktion (DE-588)4138565-2 s DE-604 B:DE-101 application/pdf http://d-nb.info/1170282113/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030763814&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lasok, Hanna Mechanical control of lateral roots development in Arabidopsis thaliana Ackerschmalwand (DE-588)4141299-0 gnd Mechanischer Reiz (DE-588)4447802-1 gnd Stressreaktion (DE-588)4138565-2 gnd Wachstum (DE-588)4064115-6 gnd Seitenwurzel (DE-588)4180779-0 gnd |
| subject_GND | (DE-588)4141299-0 (DE-588)4447802-1 (DE-588)4138565-2 (DE-588)4064115-6 (DE-588)4180779-0 (DE-588)4113937-9 |
| title | Mechanical control of lateral roots development in Arabidopsis thaliana |
| title_auth | Mechanical control of lateral roots development in Arabidopsis thaliana |
| title_exact_search | Mechanical control of lateral roots development in Arabidopsis thaliana |
| title_full | Mechanical control of lateral roots development in Arabidopsis thaliana vorgelegt von Hanna Lasok |
| title_fullStr | Mechanical control of lateral roots development in Arabidopsis thaliana vorgelegt von Hanna Lasok |
| title_full_unstemmed | Mechanical control of lateral roots development in Arabidopsis thaliana vorgelegt von Hanna Lasok |
| title_short | Mechanical control of lateral roots development in Arabidopsis thaliana |
| title_sort | mechanical control of lateral roots development in arabidopsis thaliana |
| topic | Ackerschmalwand (DE-588)4141299-0 gnd Mechanischer Reiz (DE-588)4447802-1 gnd Stressreaktion (DE-588)4138565-2 gnd Wachstum (DE-588)4064115-6 gnd Seitenwurzel (DE-588)4180779-0 gnd |
| topic_facet | Ackerschmalwand Mechanischer Reiz Stressreaktion Wachstum Seitenwurzel Hochschulschrift |
| url | http://d-nb.info/1170282113/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030763814&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT lasokhanna mechanicalcontroloflateralrootsdevelopmentinarabidopsisthaliana |
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