Gnotobiotics:
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
London, United Kingdom
Elsevier, Academic Press
[2017]
|
| Schriftenreihe: | American College of Laboratory Animal Medicine series
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | xiii, 414 Seiten Illustrationen |
| ISBN: | 9780128045619 0128045612 |
Internformat
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| 245 | 1 | 0 | |a Gnotobiotics |c edited by Trenton R. Schoeb, Kathryn A. Eaton |
| 264 | 1 | |a London, United Kingdom |b Elsevier, Academic Press |c [2017] | |
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Datensatz im Suchindex
| _version_ | 1804179054379663360 |
|---|---|
| adam_text | Contents
List of Contributors
Foreword
1. Historical Overview
Trenton R. Schoeb and Roger P. Orcutt
References
2. Principles of Establishing and
Operating a Gnotobiotic Facility
Trenton R. Schoeb, RichardJ. Rahija, Cassie Boyd,
Roger P. Orcutt and Kathryn A. Eaton
1. Introduction
2. Institutional Factors
2.1 Financial Support
2.2 Space Requirements
2.3 Autoclave
2.4 Personnel
2.5 Microbiology Laboratory
2.6 Facility Productivity and Experimental
Utilization
2.7 Facility Operation and Compliance
3. Isolator Selection
3.1 Isolator Types and Construction
3.2 Individual Positive Pressure Cage
Systems
4. Sterilization
4.1 Steam
4.2 Ionizing Radiation
4.3 Liquid Chemical
4.4 Gas and Vapor Chemical
4.5 Dry Heat
4.6 Filtration
4.7 Further Information
5. Operation
5.1 Standard Operating Procedures
5.2 Isolator Setup
5.3 Sterilization and Entry of Supplies
5.4 Introduction and Transfer of Animals
5.5 Working in Isolators
5.6 Monitoring for Contamination
5.7 Derivation of Germfree Mice 50
5.8 Establishing Defined Microbiota 51
6. Resources 52
6.1 Recommended Reading 52
6.2 Online 54
6.3 Manufacturers 54
6.4 Laboratories 55
Glossary 56
References 58
3. Procedures for Rodents
Cassie Boyd, Kathryn A. Eaton, Trenton R. Schoeb,
Alton G. Swennes, Hayden Bickerton, Billie J.
Parsons, Stacey Sinclair and Chriss Vowles
1. Introduction 66
2. Background and Acknowledgments 67
2.1 University of Texas Southwestern Medical
Center (UT Southwestern) 67
2.2 University of Michigan (Michigan) 67
2.3 University of Alabama at Birmingham
(UAB) 67
2.4 Baylor College of Medicine (BCM) 68
3. General Information 68
3.1 UT Southwestern 68
3.2 UAB 68
3.3 BCM 69
4. Sterilization and Sterilization Monitoring 71
4.1 UT Southwestern 74
4.2 UAB 75
4.3 BCM 83
5. Sterilizing Cylinders 86
5.1 UT Southwestern 86
5.2 UAB 114
5.3 BCM 125
6. Water 136
6.1 UT Southwestern 136
6.2 UAB 138
6.3 BCM 141
7. Transferring Supplies Into Isolators 141
7.1 UT Southwestern 142
7.2 UAB 161
7.3 BCM 173
ix
xi
14
22
22
22
22
25
25
25
25
26
27
27
32
32
33
34
34
37
38
38
38
38
38
38
40
45
45
46
v
vi Contents
8. Isolator Setup 183
8.1 UT Southwestern 184
8.2 Michigan 223
83 UAB 224
8.4 BCM 268
9. Husbandry 290
9.1 UAB 293
9.2 BCM 296
10. Microbiological Monitoring 298
10.1 UT Southwestern 298
10.2 Michigan 304
103 UAB 305
11. Records and Maintenance 310
11.1 UAB 311
11.2 BCM 312
12. Derivation of Germfree Mice 312
12.1 Michigan 313
12.2 UAB 313
13. Establishing Microbiota in Germfree
Mice 317
13.1 UAB 317
14. Alternatives to Isolators 318
14.1 UAB 319
15. Shipping and Receiving Mice 320
15.1 UAB 321
16. Materials Lists and Fabrication
Diagrams 323
16.1 UAB 323
References 333
4. Developing a Business Plan for Your
Gnotobiotics Program
George Langan and Betty Theriault
1. Introduction 335
2. Operational Management Considerations
in Establishing a Gnotobiotics Program 335
5. Gnotobiotic Pigs: From Establishing
Facility to Modeling Human
Infectious Diseases
Lijuan Yuan, Peter M. Jobst and Mariah Weiss
1. History of Gnotobiotic Pigs 349
2. Establishing and Operating a Gnotobiotic
Pig Facility 350
2.1 Facilities 350
2.2 Specialized Equipment 351
2.3 Isolation Systems 353
2.4 Space Requirements 354
2.5 Preparation of Isolators 354
2.6 Sow Hysterectomy 355
2.7 Feeding 358
2.8 Spraying Materials Prior to Entry Into
an Isolator 359
2.9 Sample Collection and Administration
oflnocula 359
2.10 Cleaning a Dirty Isolator 360
3. Gnotobiotic Pig Models for Studies of
Infectious Diseases 360
3.1 Gnotobiotic Pig Models for Human
Enteric Viruses 360
3.2 Gnotobiotic Pig Models for Bacterial
and Fungal Infections 362
3.3 Studies of Probiotics and Prebiotics
Using Gnotobiotic Pigs 362
3.4 Gnotobiotic Pigs Colonized With
Human Gut Microbiota 363
3.5 Concluding Remarks 363
References 364
6. Gnotobiotic Fish as Models to Study
Host-Microbe Interactions
Torunn Forbergand Kathryn Milligan-Myhre
3. Developing a Business Model 337 1. Introduction 369
3.1 Identifying the Users and Volume 2. Brief Review of Fish Gnotobiology 370
of Work 337 3. Protocol for Derivation and Husbandry
3.2 Identifying Space and Equipment of Gnotobiotic Fish 371
Requirements 339 4. Facilities 371
3.3 Labor---What Products and Services
Will Be provided and Who Will Provide 5. Considerations for Disinfection of Eggs 371
Them 341 5.1 Polyvinylpyrrolidone-Iodine 372
4. Identifying Expenses 342 5.2 Sodium Hypochlorite Solution 373
4.1 Consumable Supplies 342 5.3 Glutaraldehyde 373
4.2 Labor 344 5.4 Hydrogen Peroxide 373
4.3 Support Costs 345 5.5 Fish Husbandry in Water Containing
4.4 Quality Control Costs 346 Antimicrobial Agents 373
5. Establishing Fees 346 6. Confirmation of Sterility 374
6. Subsidization 347 6.1 Microscopic Visualization of Flask 374
7. Financial Reviews 347 6.2 Culture-Based Detection 374
8. Summary 347 6.3 PCR Amplification of the Bacteria-
References 348 Specific 16S Ribosomal RNA Gene 374
Contents vii
6.4 In Situ Hybridization 374
6.5 Flow Cytometry 375
7. Long-Term Husbandry 375
7.1 Tissue Culture Flasks 375
7.2 Aerated Bottles 376
7.3 Beakers/Tanks in Gnotobiotic Bubble 376
8. Food Considerations for Long-Term
Experiments 377
8.1 Formulated Food 377
8.2 Rotifers 377
8.3 Paramecium 377
8.4 Artemi a 377
8.5 Copepods 378
8.6 Tetrahymena 378
8.7 Algae 378
9. Other Considerations for Gnotobiotic
Fish Husbandry 378
10. Derivation of Germfree Stickleback
Embryos 379
10.1 Solutions 380
References 380
Use of Gnotobiotic Mice in the
Study of Metabolic Syndrome
Benoit Chassaing and Andrew T. Cewirtz
1. introduction 385
1.1 To What Extent Is a Microbiota
Required for Metabolic Syndrome? 386
1.2 Is an Altered Microbiota Sufficient
to Drive Metabolic Syndrome? 387
2. Conclusion and Future Directions 389
Acknowledgments 389
References 389
8. Gnotobiotics and Inflammatory
Bowel Disease
Trenton R. Schoeb
1. Introduction 391
2. Cytokines and Cytokine Signaling 394
2.1 Interleukin-10 394
2.2 Interleukin-2 395
2.3 TGF-(31 396
2.4 TNF-a 396
2.5 Other Cytokines 396
3. T-Cell Receptors and MHC 396
3.1 T-Cell Receptor and MF1C 11 Deficient
Mice 396
3.2 HLA B27 p2m Transgenic Rats 397
4. Other Mutants 397
4.1 Core 1-Derived O-Glycan Deficiency 397
4.2 Fas-Associated via Death Domain
Protein 397
4.3 Glutathione Peroxidase Deficiency 397
4.4 Nfil3 Deficiency 397
4.5 PI3 Kinase p1106 Deficiency 398
4.6 Telomerase Deficiency 398
4.7 T-Bet Deficiency 398
4.8 TLR5 Deficiency 398
5. T-Cell Transfer 399
6. Inbred Strains 401
6.1 SAMP1/Yit 401
6.2 C3H/HeJBir 401
7. Conclusion 401
References 401
Index 411
The expanding interest in the role of the microbiota in health and disease is driving a rapidly expanding
interest in establishing and operating gnotobiotic facilities.
This text reviews how gnotobiotic animals of various species are or can be used to study the role of the
microbiota in various areas of research of current interest. These fields range from metabolism (nutrition,
obesity obesity-related diseases like type-2 diabetes, hypertension, and cardiovascular disease) and
cancer, to diseases of dysregulated immunity and inflammation such as inflammatory bowel diseases
(Crohn s disease and ulcerative colitis).
This is the first readily accessible source of detailed operational protocols, discussions of required
infrastructure and support, and common problems and solutions, which are necessary to establish and
successfully operate a gnotobiotic facility.
Gnotobiotics provides readers with a comprehensive guide to scientific application of gnotobiotic
technology.
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| spelling | Gnotobiotics edited by Trenton R. Schoeb, Kathryn A. Eaton London, United Kingdom Elsevier, Academic Press [2017] xiii, 414 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier American College of Laboratory Animal Medicine series Gnotobiologie (DE-588)4157779-6 gnd rswk-swf Gnotobiotik (DE-588)4294572-0 gnd rswk-swf Gnotobiotik (DE-588)4294572-0 s Gnotobiologie (DE-588)4157779-6 s DE-604 Schoeb, Trenton R. edt Eaton, Kathryn A. edt Erscheint auch als Online-Ausgabe 978-0-12-804583-1 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030668256&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030668256&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
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| subject_GND | (DE-588)4157779-6 (DE-588)4294572-0 |
| title | Gnotobiotics |
| title_auth | Gnotobiotics |
| title_exact_search | Gnotobiotics |
| title_full | Gnotobiotics edited by Trenton R. Schoeb, Kathryn A. Eaton |
| title_fullStr | Gnotobiotics edited by Trenton R. Schoeb, Kathryn A. Eaton |
| title_full_unstemmed | Gnotobiotics edited by Trenton R. Schoeb, Kathryn A. Eaton |
| title_short | Gnotobiotics |
| title_sort | gnotobiotics |
| topic | Gnotobiologie (DE-588)4157779-6 gnd Gnotobiotik (DE-588)4294572-0 gnd |
| topic_facet | Gnotobiologie Gnotobiotik |
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