Verfügbarkeit: The immature HIV-1 capsid and its assembly

The immature HIV-1 capsid and its assembly:

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Bibliographische Detailangaben
1. Verfasser:	Obr, Martin (VerfasserIn)
Format:	Abschlussarbeit Buch
Sprache:	English
Veröffentlicht:	Heidelberg 2017
Schlagworte:	Hochschulschrift
Online-Zugang:	Inhaltsverzeichnis Inhaltsverzeichnis
Beschreibung:	119 Seiten Illustrationen, Diagramme 30 cm

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MARC


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Datensatz im Suchindex

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adam_text	TABLE OF CONTENTS 1. IN TRO D U C TIO N ............................................................................................................11 1.1. RETROVIRUSES AND THEIR CLASSIFICATION.............................................................11 1.1.1. HIV-L GENOME..................................................................................................... 13 1.2. HIV-1 LIFE CYCLE................................................................................................... 15 1.2.1. EARLY PHASE......................................................................................................... 16 1.2.2. LATE PHASE...........................................................................................................18 1.3. THE HIV-1 PARTICLE............................................................................................. 19 1.3.1. ARCHITECTURE OF THE IMMATURE VIRION...............................................................19 1.3.2. MATURATION AND THE MATURE HIV-1 CORE ......................................................... 23 1.4. HIV-1 INHIBITORS TARGETING C A ..........................................................................24 1.4.1. ASSEMBLY INHIBITORS........................................................................................... 25 1.4.2. MATURATION INHIBITORS........................................................................................26 1.4.3. POST-ENTRY INHIBITORS TARGETING CA................................................................... 27 1.5. IN VITRO SYSTEMS OF HIV-1 ASSEMBLY ................................................................ 27 1.5.1. MATURE CAPSID MODELS.......................................................................................28 1.5.2. IMMATURE MODELS AND PROPOSED SWITCHING MECHANISMS ............................ 28 2. TH ESIS A LM S ...........................................................................................................31 3. M ATERIALS AND M E TH O D S ......................................................................................32 3.1. MATERIALS............................................................................................................. 32 3.1.1. CHEMICALS AND CONSUMABLES............................................................................32 3.1.2. ENZYMES...............................................................................................................33 3.1.3. BUFFER RECIPIES....................................................................................................34 3.1.4. INSTRUMENTS........................................................................................................ 35 3.2. METHODS.............................................................................................................. 37 3.2.1. DMA METHODS 37 3.2.3. BVM QUANTIFICATION...........................................................................................42 3.2.4. CELL CULTURE METHODS ........................................................................................ 43 4. R E S U LTS .................................................................................................................... 44 4.1. STRUCTURAL AND BIOCHEMICAL ANALYSIS OF MATURATION INHIBITOR BINDING TO IMMATURE CA ......................................................................................................44 4.1.1. ESTABLISHING THE IN VITRO SYSTEM FOR ML BINDING ............................................. 45 4.1.2. QUANTITATIVE DETERMINATION OF BVM IN VIPS ................................................. 48 4.1.3. ASSESSMENT OF BVM ACTIVITY IN VITRO................................................................51 4.1.4. STRUCTURAL ANALYSIS AND VERIFICATION OF BVM BINDING WITHIN CASP1 LATTICE.................................................................................................................. 54 4.2. DECIPHERING THE ROLE OF CA-NTD IN THE ASSEMBLY OF IMMATURE HIV-1 PARTICLE .................................................... 55 4.2.1. MUTATIONAL ANALYSIS OF CA-NTD RESIDUES CRITICAL FOR IMMATURE PARTICLE FORMATION............................................................................................................55 4.2.2. THE STRUCTURE OF AMACANCSP2 E75A/E76A TUBES........................................64 4.2.3. MUTATIONAL ANALYSIS OF AN IMMATURE INTERFACE IN CA-NTD ........................... 65 4.2.4. RESCUE OF THE IMMATURE ASSEMBLY OF THE R18A AND E75A/E76A MUTATIONS...........................................................................................................68 4.2.5. INFLUENCE OF PH ON CA LATTICE ASSEMBLY .......................................................... 71 4.3. GENETICALLY ENCODED LABELING OF CA IN VITRO ................................................. 73 4.3.1. EXPRESSION OF AMBER MUTANTS......................................................................... 76 4.3.2. IN VITRO ASSEMBLY OF AMBER MUTANTS .............................................................. 77 4.3.3. LABELING OF AMBER MUTANTS..............................................................................78 4.3.4. DEVELOPMENT OF IN VITRO MODEL FOR THE CONICAL CAPSID OF HIV-1 ................. 80 4.3.5. THE CONICAL IN VITRO ASSEMBLY SYSTEM OF CA .................................................. 81 4.3.6. THE CONICAL IN VITRO ASSEMBLY SYSTEM OF CANC 82 5. D IS C U S S IO N .............................................................................................................86 5.1. BVM BINDING POCKET..........................................................................................86 5.2. IMMATURE HIV-1 AND ITS ASSEMBLY ................................................................. 89 5.3. GENETICALLY ENCODED LABELING OF CA AND MATURE CORE IN VITRO MODEL.... 95 6. C O N CLUSION AND FU TU RE D IRE C TIO N S ................................................................. 98 6.1. MATURATION INHIBITOR BINDING POCKET .............................................................. 98 6.2. IMMATURE HIV-1 CAPSID AND ITS ASSEM BLY ................................................... 98 6.3. GENETICALLY-ENCODED HIV-1 CA LABELING.......................................................99 APPENDIX I -ABBREVIATIONS USED IN THIS TEXT ....................................................................... 100 APPENDIX II -OLIGONUCLEOTIDE SEQUENCES USED IN THIS WORK ............................................ 103 R E FE RE N C E S ...........................................................................................................................106 LIST O F P U B LIC A TIO N S ..........................................................................................................119
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spelling	Obr, Martin Verfasser aut The immature HIV-1 capsid and its assembly presented by Ing. Mgr. Martin Obr Heidelberg 2017 119 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Ruperto-Carola University of Heidelberg 2017 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1160107017/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030665574&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Obr, Martin The immature HIV-1 capsid and its assembly
subject_GND	(DE-588)4113937-9
title	The immature HIV-1 capsid and its assembly
title_auth	The immature HIV-1 capsid and its assembly
title_exact_search	The immature HIV-1 capsid and its assembly
title_full	The immature HIV-1 capsid and its assembly presented by Ing. Mgr. Martin Obr
title_fullStr	The immature HIV-1 capsid and its assembly presented by Ing. Mgr. Martin Obr
title_full_unstemmed	The immature HIV-1 capsid and its assembly presented by Ing. Mgr. Martin Obr
title_short	The immature HIV-1 capsid and its assembly
title_sort	the immature hiv 1 capsid and its assembly
topic_facet	Hochschulschrift
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