Holdings: Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro

Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro:

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Bibliographic Details
Main Author:	Maru, Helina 1991- (Author)
Format:	Thesis Book
Language:	English
Published:	Köln 23. Februar 2018
Subjects:	Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Item Description:	Text englisch. Zusammenfassung in deutscher und englischer Sprache
Physical Description:	87 Seiten Illustrationen 21 cm

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MARC


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adam_text	TABLE OF CONTENTS GLOSSARY....................................................................................................................................... 9 INTRODUCTION................................................................................................................................ 11 1.1 LINEZOLID.....................................................................................................................11 1.1.1 MECHANISM OF ACTION........................................................................................11 1.1.2 LINEZOLID-INDUCED PERIPHERAL NEUROPATHY....................................................12 1.2 THE PERIPHERAL NERVOUS SYSTEM..............................................................................14 1.2.1 NEURONS AND PERIPHERAL NERVES.......................................................................14 1.2.2 SCHWANN CELLS..................................................................................................... 16 1.3 INTRACELLULAR CA2+-HOMEOSTASIS................................................................................ 19 1.3.1 MECHANISMS OF CA2+-HOMEOSTASIS................................................................. 19 1.3.2 CA2+-HOMEOSTASIS IN NEURONAL LESIONS AND NEURONAL DEGENERATION .......... 20 1.4 AIMS OF THIS THESIS......................................................................................................23 2 METHODS.............................................................................................................................24 2.1 ANIMALS.......................................................................................................................24 2.2 PRIMARY CELL CULTURES................................................................................................24 2.2.1 COLLAGEN COATING OF CULTURE DISHES ............................................................... 24 2.2.2 SCHWANN CELLS................................................................................................... 25 2.2.3 DRG-NEURON CULTURES ...................................................................................... 26 2.3 STORAGE AND DILUTION OF THE DRUGS..........................................................................27 2.4 ASSESSMENT OF CELL SURVIVAL AND MORPHOLOGICAL CHANGES IN VITRO ................... 27 2.4.1 VIABILITY OF SCHWANN CELLS UNDER LINEZOLID-TREATMENT ................................. 27 2.4.2 IMMUNOFLUORESCENCE STAINING ....................................................................... 29 2.5 MICROPLATE ASSAYS.................................................................................................... 30 2.5.1 CA2+-IMAGING.. 2.5.2 MITOTRACKER MICROPLATE-ASSAY ....... 2.5.3 LUMINESCENT ATP DETECTION ASSAY .............................................................. 33 2.6 REAL-TIME PCR OF GROWTH FACTOR LEVELS IN SCHWANN CELL CULTURES .................... 33 2.7 STATISTICAL ANALYSIS....................................................................................................38 2.8 REAGENTS AND EQUIPMENT.......................................................................................39 2.8.1 DRUGS...................................................................................................................39 2.8.2 REAGENTS FOR CELL CULTURE AND DRG EXTRACTION............................................39 2.8.3 REAGENTS FOR IMMUNOFLUORESCENCE .............................................................. 40 2.8.4 ANTIBODIES.......................................................................................................... 40 3 RESULTS............................................................................................................................... 43 3.1 HIGH DOSAGES OF LINEZOLID REDUCE SCHWANN CELL VIABILITY..................................43 3.2 LINEZOLID-TREATMENT REDUCES NEU RITE OUTGROWTH OF DRG-NEURONS ................... 45 3.3 INTRACELLULAR CA2+-LEVELS IN SCHWANN CELLS AND DRG-NEURONS ARE INCREASED AFTER LINEZOLID-TREATMENT.......................................................................................................46 3.4 DRG-NEURONS SHOW A REDUCED MITOCHONDRIAL MEMBRANE POTENTIAL AFTER LINEZOLID-TREATMENT................................................................................................................ 47 3.5 INTRACELLULAR ATP IS NOT DECREASED AFTER LINEZOLID-TREATMENT ........................... 49 3.6 EXPRESSION OF BDNF IN SCHWANN CELLS IS ENHANCED AFTER 72H LINEZOLID- TREATMENT.................................................................................................................................50 3.7 KB-R7943 PARTIALLY PREVENTS LINEZOLID-INDUCED DECREASE IN NEUNTE OUTGROWTH51 3.8 KB-R7943 REDUCES INTRACELLULAR CA2+-LEVELS IN SCHWANN CELLS TREATED WITH LINEZOLID...................................................................................................................................53 4 DISCUSSION..........................................................................................................................55 4.1 INCREASED MORTALITY OF SCHWANN CELLS AFTER LINEZOLID-TREATMENT ....................... 55 4.2 NEURITE OUTGROWTH OF DRG-NEURONS AFTER LINEZOLID-TREATMENT...........................56 4.3 INTRACELLULAR CA2+-LEVELS AFTER LINEZOLID-TREATMENT ............................................... 57 4.4 MITOCHONDRIAL FUNCTION IN NEURONS AFTER LINEZOLID-TREATMENT .............................. 58 4.4.1 MITOCHONDRIAL MEMBRANE-POTENTIAL IN DRG-NEURONS AND SCHWANN CELLS AFTER LINEZOLID-TREATMENT................................................................................................... 59 4.4.2 ATR-FLUORESCENCE IN DRG-NEURONS AND SCHWANN CELLS AFTER LINEZOLID- TREATMENT.............................................................................................................................60 4.5 GROWTH FACTOR EXPRESSION IN SC CULTURES AFTER LINEZOLID-TREATMENT ................ 61 4.6 BLOCKING REVERSE NA+-CA2+-EXCHANGE..................................................................64 4.6.1 REVERSE NCX-OPERATION IN LINEZOLID-INDUCED NEURITE OUTGROWTH ARREST .65 4.6.2 THE EFFECT OF BLOCKING NCX-REVERSE-OPERATION THE INTRACELLULAR CA2+- CONCENTRATION IN SCHWANN CELLS AFTER LINEZOLID-TREATMENT ............................................ 65 4.7 LIMITATIONS AND OUTLOOK OF THIS THESIS..................................................................66 5 SUMMARY.......................................................................................................................... 68 6 ZUSAMMENFASSUNG.......................................................................................................... 70 7 REFERENCES........................................................................................................................ 72 8 PRELIMINARY PUBLICATIONS.................................................................................................80 9 APPENDIX........................................................................................................................... 81 9.1 LIST OF FIGURES........................................................................................................... 81 9.2 AUSFUEHRLICHE ZUSAMMENFASSUNG DER ERGEBNISSE IN DEUTSCHER SPRACHE ...... 82 9.2.1 FRAGESTELLUNG UND ZIELSETZUNG DER ARBEIT....................................................62 9.2.2 UEBERLEBEN VON SCHWANN-ZELLEN....................................................................82 9.2.3 NEURITENWACHSTUM SENSIBLER NEURONE ........................................................ 83 9.2.4 VERAENDERUNGEN DER INTRAZELLULAEREN KALZIUMKONZENTRATION UNTER LINEZOLIDTHERAPIE...............................................................................................................63 9.2.5 EINFLUSS DER LINEZOLIDTHERAPIE AUF DIE FUNKTION DER MITOCHONDRIEN ........ 83 9.2.6 EXPRESSION VON WACHSTUMSFAKTOREN UNTER LINEZOLIDTHERAPIE ................. 84 9.2.7 ROLLE DES NATRIUM-KALZIUM-AUSTAUSCHERS BEI LINEZOLID-INDUZIERTEN VERAENDERUNGEN DES ZELLULAEREN KALZIUMHAUSHALTS......................................................65 10 LEBENSLAUF........................................................................................................................ 66
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spelling	Maru, Helina 1991- Verfasser (DE-588)1157038123 aut Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro vorgelegt von Helina Maru Köln 23. Februar 2018 87 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Text englisch. Zusammenfassung in deutscher und englischer Sprache Dissertation Universität zu Köln 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1157132200/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030650230&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Maru, Helina 1991- Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro
subject_GND	(DE-588)4113937-9
title	Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro
title_auth	Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro
title_exact_search	Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro
title_full	Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro vorgelegt von Helina Maru
title_fullStr	Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro vorgelegt von Helina Maru
title_full_unstemmed	Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro vorgelegt von Helina Maru
title_short	Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro
title_sort	effekte von linezolid auf zellen des peripheren nervensystems in vitro
topic_facet	Hochschulschrift
url	http://d-nb.info/1157132200/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030650230&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT maruhelina effektevonlinezolidaufzellendesperipherennervensystemsinvitro

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