Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro:
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Format: | Abschlussarbeit Buch |
Sprache: | English |
Veröffentlicht: |
Köln
23. Februar 2018
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Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
Beschreibung: | Text englisch. Zusammenfassung in deutscher und englischer Sprache |
Beschreibung: | 87 Seiten Illustrationen 21 cm |
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245 | 1 | 0 | |a Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro |c vorgelegt von Helina Maru |
264 | 1 | |a Köln |c 23. Februar 2018 | |
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502 | |b Dissertation |c Universität zu Köln |d 2018 | ||
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Datensatz im Suchindex
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adam_text | TABLE OF CONTENTS
GLOSSARY.......................................................................................................................................
9
INTRODUCTION................................................................................................................................
11
1.1
LINEZOLID.....................................................................................................................11
1.1.1 MECHANISM OF
ACTION........................................................................................11
1.1.2 LINEZOLID-INDUCED PERIPHERAL
NEUROPATHY....................................................12
1.2 THE PERIPHERAL NERVOUS
SYSTEM..............................................................................14
1.2.1 NEURONS AND PERIPHERAL
NERVES.......................................................................14
1.2.2 SCHWANN
CELLS.....................................................................................................
16
1.3 INTRACELLULAR
CA2+-HOMEOSTASIS................................................................................
19
1.3.1 MECHANISMS OF
CA2+-HOMEOSTASIS.................................................................
19
1.3.2 CA2+-HOMEOSTASIS IN NEURONAL LESIONS AND NEURONAL DEGENERATION
..........
20
1.4 AIMS OF THIS
THESIS......................................................................................................23
2
METHODS.............................................................................................................................24
2.1
ANIMALS.......................................................................................................................24
2.2 PRIMARY CELL
CULTURES................................................................................................24
2.2.1 COLLAGEN COATING OF CULTURE DISHES
...............................................................
24
2.2.2 SCHWANN
CELLS...................................................................................................
25
2.2.3 DRG-NEURON CULTURES
......................................................................................
26
2.3 STORAGE AND DILUTION OF THE
DRUGS..........................................................................27
2.4 ASSESSMENT OF CELL SURVIVAL AND MORPHOLOGICAL CHANGES IN VITRO
...................
27
2.4.1 VIABILITY OF SCHWANN CELLS UNDER LINEZOLID-TREATMENT
.................................
27
2.4.2 IMMUNOFLUORESCENCE STAINING
.......................................................................
29
2.5 MICROPLATE
ASSAYS....................................................................................................
30
2.5.1 CA2+-IMAGING..
2.5.2 MITOTRACKER MICROPLATE-ASSAY
.......
2.5.3 LUMINESCENT ATP DETECTION ASSAY
..............................................................
33
2.6 REAL-TIME PCR OF GROWTH FACTOR LEVELS IN SCHWANN CELL CULTURES
....................
33
2.7 STATISTICAL
ANALYSIS....................................................................................................38
2.8 REAGENTS AND
EQUIPMENT.......................................................................................39
2.8.1
DRUGS...................................................................................................................39
2.8.2 REAGENTS FOR CELL CULTURE AND DRG
EXTRACTION............................................39
2.8.3 REAGENTS FOR IMMUNOFLUORESCENCE
..............................................................
40
2.8.4
ANTIBODIES..........................................................................................................
40
3
RESULTS...............................................................................................................................
43
3.1 HIGH DOSAGES OF LINEZOLID REDUCE SCHWANN CELL
VIABILITY..................................43
3.2 LINEZOLID-TREATMENT REDUCES NEU RITE OUTGROWTH OF DRG-NEURONS
...................
45
3.3 INTRACELLULAR CA2+-LEVELS IN SCHWANN CELLS AND DRG-NEURONS ARE
INCREASED
AFTER
LINEZOLID-TREATMENT.......................................................................................................46
3.4 DRG-NEURONS SHOW A REDUCED MITOCHONDRIAL MEMBRANE POTENTIAL AFTER
LINEZOLID-TREATMENT................................................................................................................
47
3.5 INTRACELLULAR ATP IS NOT DECREASED AFTER LINEZOLID-TREATMENT
...........................
49
3.6 EXPRESSION OF BDNF IN SCHWANN CELLS IS ENHANCED AFTER 72H LINEZOLID-
TREATMENT.................................................................................................................................50
3.7 KB-R7943 PARTIALLY PREVENTS LINEZOLID-INDUCED DECREASE IN NEUNTE
OUTGROWTH51
3.8 KB-R7943 REDUCES INTRACELLULAR CA2+-LEVELS IN SCHWANN CELLS TREATED
WITH
LINEZOLID...................................................................................................................................53
4
DISCUSSION..........................................................................................................................55
4.1 INCREASED MORTALITY OF SCHWANN CELLS AFTER LINEZOLID-TREATMENT
.......................
55
4.2 NEURITE OUTGROWTH OF DRG-NEURONS AFTER
LINEZOLID-TREATMENT...........................56
4.3 INTRACELLULAR CA2+-LEVELS AFTER LINEZOLID-TREATMENT
...............................................
57
4.4 MITOCHONDRIAL FUNCTION IN NEURONS AFTER LINEZOLID-TREATMENT
..............................
58
4.4.1 MITOCHONDRIAL MEMBRANE-POTENTIAL IN DRG-NEURONS AND SCHWANN CELLS
AFTER
LINEZOLID-TREATMENT...................................................................................................
59
4.4.2 ATR-FLUORESCENCE IN DRG-NEURONS AND SCHWANN CELLS AFTER LINEZOLID-
TREATMENT.............................................................................................................................60
4.5 GROWTH FACTOR EXPRESSION IN SC CULTURES AFTER LINEZOLID-TREATMENT
................
61
4.6 BLOCKING REVERSE
NA+-CA2+-EXCHANGE..................................................................64
4.6.1 REVERSE NCX-OPERATION IN LINEZOLID-INDUCED NEURITE OUTGROWTH
ARREST .65
4.6.2 THE EFFECT OF BLOCKING NCX-REVERSE-OPERATION THE INTRACELLULAR
CA2+-
CONCENTRATION IN SCHWANN CELLS AFTER LINEZOLID-TREATMENT
............................................
65
4.7 LIMITATIONS AND OUTLOOK OF THIS
THESIS..................................................................66
5
SUMMARY..........................................................................................................................
68
6
ZUSAMMENFASSUNG..........................................................................................................
70
7
REFERENCES........................................................................................................................
72
8 PRELIMINARY
PUBLICATIONS.................................................................................................80
9
APPENDIX...........................................................................................................................
81
9.1 LIST OF
FIGURES...........................................................................................................
81
9.2 AUSFUEHRLICHE ZUSAMMENFASSUNG DER ERGEBNISSE IN DEUTSCHER SPRACHE
......
82
9.2.1 FRAGESTELLUNG UND ZIELSETZUNG DER
ARBEIT....................................................62
9.2.2 UEBERLEBEN VON
SCHWANN-ZELLEN....................................................................82
9.2.3 NEURITENWACHSTUM SENSIBLER NEURONE
........................................................
83
9.2.4 VERAENDERUNGEN DER INTRAZELLULAEREN KALZIUMKONZENTRATION UNTER
LINEZOLIDTHERAPIE...............................................................................................................63
9.2.5 EINFLUSS DER LINEZOLIDTHERAPIE AUF DIE FUNKTION DER MITOCHONDRIEN
........
83
9.2.6 EXPRESSION VON WACHSTUMSFAKTOREN UNTER LINEZOLIDTHERAPIE
.................
84
9.2.7 ROLLE DES NATRIUM-KALZIUM-AUSTAUSCHERS BEI LINEZOLID-INDUZIERTEN
VERAENDERUNGEN DES ZELLULAEREN
KALZIUMHAUSHALTS......................................................65
10
LEBENSLAUF........................................................................................................................
66
|
any_adam_object | 1 |
author | Maru, Helina 1991- |
author_GND | (DE-588)1157038123 |
author_facet | Maru, Helina 1991- |
author_role | aut |
author_sort | Maru, Helina 1991- |
author_variant | h m hm |
building | Verbundindex |
bvnumber | BV045262301 |
ctrlnum | (OCoLC)1136273521 (DE-599)DNB1157132200 |
discipline | Medizin |
format | Thesis Book |
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spelling | Maru, Helina 1991- Verfasser (DE-588)1157038123 aut Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro vorgelegt von Helina Maru Köln 23. Februar 2018 87 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Text englisch. Zusammenfassung in deutscher und englischer Sprache Dissertation Universität zu Köln 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1157132200/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030650230&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Maru, Helina 1991- Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro |
subject_GND | (DE-588)4113937-9 |
title | Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro |
title_auth | Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro |
title_exact_search | Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro |
title_full | Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro vorgelegt von Helina Maru |
title_fullStr | Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro vorgelegt von Helina Maru |
title_full_unstemmed | Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro vorgelegt von Helina Maru |
title_short | Effekte von Linezolid auf Zellen des peripheren Nervensystems in vitro |
title_sort | effekte von linezolid auf zellen des peripheren nervensystems in vitro |
topic_facet | Hochschulschrift |
url | http://d-nb.info/1157132200/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030650230&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
work_keys_str_mv | AT maruhelina effektevonlinezolidaufzellendesperipherennervensystemsinvitro |
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