Verfügbarkeit: Supramolecular structure of visual pigments in chicken photoreceptors

Supramolecular structure of visual pigments in chicken photoreceptors:

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Bibliographische Detailangaben
1. Verfasser:	Tan, Zhiyang 1989- (VerfasserIn)
Format:	Abschlussarbeit Buch
Sprache:	English
Veröffentlicht:	Köln [2018]
Schlagworte:	Hochschulschrift
Online-Zugang:	Inhaltsverzeichnis Inhaltsverzeichnis
Beschreibung:	Zusammenfassung in deutscher und englischer Sprache. Seiten 1-12 in römischer Zählung
Beschreibung:	127 Seiten Illustrationen 30 cm

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MARC


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adam_text	CONTENTS LIST OF FIGURES LIST OF TABLES............................................................................................................................ VIII ABBREVIATIONS............................................................................................................................ IX 1 INTRODUCTION......................................................................................................................... 13 1.1 AVIAN POLARIZATION VISION.............................................................................................13 1.2 RODS AND CONES............................................................................................................ 18 1.3 AIM.................................................................................................................................24 1.4 TRANSMISSION ELECTRON MICROSCOPY (TEM) ................................................................ 24 1.4.1 SAMPLE PREPARATION.................................................................................................... 27 1.4.2 ELECTRON TOMOGRAPHY (ET) .......................................................................................... 30 1.4.3 TEM MONTAGING MAPPING (MAP)................................................................................34 2 MATERIALS AND METHODS..................................................................................................... 36 2.1 CHEMICALS AND CONSUMABLES...................................................................................... 36 2.2 ANIMALS......................................................................................................................... 37 2.3 INSTRUMENTS...................................................................................................................37 2.3.1 ELECTRON MICROSCOPES.................................................................................................. 38 2.3.2 IMAGE PROCESSING.........................................................................................................39 2.4 SAMPLE PREPARATION.................................................................................................... 39 2.4.1 ANIMAL DISSECTION.........................................................................................................39 2.4.2 CHEMICAL FIXATION..........................................................................................................40 2.4.3 HIGH-PRESSURE FREEZING................................................................................................42 2.4.4 FREEZE SUBSTITUTION..................................................................................................... 43 2.4.5 ULTRA-THIN SECTIONING AT ROOM TEMPERATURE (RT) ..................................................... 44 2.4.6 CRYO-SECTIONING.............................................................................................................45 2.4.7 HEAVY-METAL STAINING (POST-STAINING)........................................................................46 2.4.8 FIDUCIAL MARKER............................................................................................................. 47 2.5 TRANSMISSION ELECTRONIC MICROSCOPY (TEM) METHOD...............................................48 2.5.1 PREPARATION FOR ROOM TEMPERATURE TEM (RT-TEM).................................................48 2.5.2 PREPARATION FOR CRYO-TEM.......................................................................................... 49 2.5.3 MONTAGE IMAGES ACQUIRED FROM RT-TEM AND CRYO-TEM ........................................ 49 2.5.4 CRYO-ELECTRON TOMOGRAPHY (CRYO-ET)........................................................................50 2.6 DATA PROCESSING............................................................................................................ 51 2.6.1 MAP STITCHING...............................................................................................................51 2.6.2 RECONSTRUCTION OF TOMOGRAMS .................................................................................. 51 2.6.3 3D SUB-TOMOGRAM AVERAGING.....................................................................................52 3 RESULTS................................................................................................................................. 54 3.1 DISTINGUISHING CONES AND RODS IN TEM MICROGRAPHS................................................54 3.1.1 CRITICAL ROLE OF THE RETINA EPITHELIA PIGMENT (RPE)..................................................57 3.1.2 FORMATION OF VESICLES INSIDE THE OUTER SEGMENTS...................................................59 3.1.3 CONNECTION BETWEEN INNER SEGMENTS AND OUTER SEGMENTS OF PHOTORECEPTORS..63 3.1.4 A SPECIFIC FEATURE IN CONES OUTER SEGMENTS (COSS): MERGED PLASMA MEMBRANE?! 3.2 CRYO-ELECTRON TOMOGRAPHY (CRYO-ET)...................................................................... 73 3.2.1 DOES THE RECORDING STRATEGY OF A TOMOGRAM INFLUENCE THE DATA QUALITY? ............. 76 3.2.2 STATISTIC SUMMARY OF CRYO-ET DATA ACQUISITION ........................................................ 79 3.2.3 SUPRAMOLECULAR STRUCTURE OF VISUAL PIGMENTS ......................................................... 81 3.2.4 SUB-TOMOGRAM AVERAGING........................................................................................... 85 4 DISCUSSION............................................................................................................................90 4.1 THE BENEFIT AND LIMITATION OF CRYO-ET ...................................................................... 90 4.1.1 PROBLEMS FROM SAMPLE PREPARATION.......................................................................... 91 4.1.2 THE LIMITATION BY CRYO-ET TECHNIQUE.........................................................................94 4.2 VESICLE STRUCTURE IN PHOTORECEPTORS........................................................................101 4.3 REPEATED CYCLE OF MERGED AND ISOLATED PLASMA MEMBRANE ............................... 105 4.4 THE SUPRAMOLECULAR STRUCTURES OF VISUAL PIGMENTS IN PHOTORECEPTORS .............. 106 5 REFERENCES.........................................................................................................................I L L
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author	Tan, Zhiyang 1989-
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spelling	Tan, Zhiyang 1989- Verfasser (DE-588)1162547952 aut Supramolecular structure of visual pigments in chicken photoreceptors vorgelegt von Zhiyang Tan Köln [2018] 127 Seiten Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Zusammenfassung in deutscher und englischer Sprache. Seiten 1-12 in römischer Zählung Dissertation Universität zu Köln 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1163734519/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030613265&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Tan, Zhiyang 1989- Supramolecular structure of visual pigments in chicken photoreceptors
subject_GND	(DE-588)4113937-9
title	Supramolecular structure of visual pigments in chicken photoreceptors
title_auth	Supramolecular structure of visual pigments in chicken photoreceptors
title_exact_search	Supramolecular structure of visual pigments in chicken photoreceptors
title_full	Supramolecular structure of visual pigments in chicken photoreceptors vorgelegt von Zhiyang Tan
title_fullStr	Supramolecular structure of visual pigments in chicken photoreceptors vorgelegt von Zhiyang Tan
title_full_unstemmed	Supramolecular structure of visual pigments in chicken photoreceptors vorgelegt von Zhiyang Tan
title_short	Supramolecular structure of visual pigments in chicken photoreceptors
title_sort	supramolecular structure of visual pigments in chicken photoreceptors
topic_facet	Hochschulschrift
url	http://d-nb.info/1163734519/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030613265&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT tanzhiyang supramolecularstructureofvisualpigmentsinchickenphotoreceptors

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