Holdings: Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing

$Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing$

Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing:

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Bibliographic Details
Main Author:	Prystaz, Katja 1988- (Author)
Format:	Thesis Book
Language:	English
Published:	Ulm [2018]
Subjects:	Interleukin 6 Knochenbruch Osteoblast Frakturheilung Fractures, Bone. Fracture healing. Osteoblasts. Osteoclasts. Interleukin-6. Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Physical Description:	118 Blätter Illustrationen, Diagramme 30 cm

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MARC


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Record in the Search Index

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adam_text	TABLE OF CONTENTS 1. INTRODUCTION................................................................................................................................9 1.1 BONE METABOLISM................................................................................................................... 9 1.1.1 THE OSTEOBLASTS AND OSTEOCLASTS .............................................................................. 10 1.2 FRACTURE HEALING................................................................................................................... 12 1.2.1 INFLAMMATORY PHASE......................................................................................................12 1.2.2 REPAIR PHASE.................................................................................................................13 1.2.3 REMODELLING PHASE.......................................................................................................13 1.3 IL-6 SIGNALLING........................................................................................................................14 1.3.1 IL-6.................................................................................................................................. 14 1.3.2 IL-6 CLASSIC AND TRANS-SIGNALLING.................................................................................16 1.3.3 INTRACELLULAR SIGNALLING OF IL-6 ..................................................................................... 17 1.3.4 PRO- AND ANTI-INFLAMMATORY EFFECTS OF IL-6................................................................19 1.3.5 BLOCKING STRATEGIES OF IL-6 SIGNALLING ....................................................................... 21 1.4 IL-6 AND BONE METABOLISM..................................................................................................25 1.4.1 BONE PHENOTYPES OF GENETICALLY MODIFIED MICE ...................................................... 25 1.4.2 INFLUENCE OF IL-6 ON OSTEOBLASTS ................................................................................ 27 1.4.3 INFLUENCE OF IL-6 ON OSTEOCLASTS ................................................................................ 27 1.5 IL-6 AND FRACTURE HEALING....................................................................................................26 1.6 AIM OF THE STUDY...................................................................................................................29 2. MATERIALS AND METHODS..........................................................................................................30 2.1 MATERIALS............................................................................................................................... 30 2.1.1 ANIMALS..........................................................................................................................30 2.1.2 CONSUMABLES............................................................................................................... 30 2.1.3 CHEMICALS.....................................................................................................................31 2.1.4 BUFFERS AND SOLUTIONS...................................................................................................33 2.1.5 CELL LINES, MEDIA AND REAGENTS FOR CELL CULTURE ....................................................... 36 2.1.6 ANTIBODIES AND CONJUGATES........................................................................................ 38 2.1.7 PRIMERS.......................................................................................................................... 40 2.1.8 KITS ................................................................... ,...........................................................40 2.1.9 EQUIPMENT.....................................................................................................................41 2.1.10 SOFTWARE......................................................................................................................43 2.2 METHODS................................................................................................................................ 43 2.2.1 ANIMAL HOUSING AND STUDY DESIGN.............................................................................43 2.2.2 ANALGESIA, ANAESTHESIA AND PREOPERATIVE PROCEDURE............................................44 2.2.3 FEMUR OSTEOTOMY........................................................................................................ 45 2.2.4 EUTHANASIA AND SAMPLE COLLECTION............................................................................46 2.2.5 LIVER HOMOGENATES.....................................................................................................46 2.2.6 MULTIPLEX ANALYSIS OF PLASMA AND HEMATOMA HOMOGENATES ............................... 47 2.2.7 WESTERN BLOTS.............................................................................................................. 47 2.2.8 FLUORESCENT-ACTIVATED CELL SORTING (FACS)-ANALYSIS ............................................... 48 2.2.9 HISTOLOGY....................................................................................................................... 49 2.2.10 BIOMECHANICAL TESTING OF FEMURS............................................................................. 52 2.2.11 MICRO-COMPUTED TOMOGRAPHY OF FEMURS.................................................................53 2.2.12 PRIMARY CELL ISOLATION AND CELL CULTURE MAINTENANCE ............................................. 54 2.2.13 GENE AND PROTEIN ANALYSES OF IL-6-STIMULATED RAW264.7 CELLS ...................... 54 2.2.14 GENE AND PROTEIN ANALYSES OF IL-6-STIMULATED OSTEOBLASTS ................................ 56 2.2.15 IMMUNOFLUORESCENCE STAINING..................................................................................57 2.2.16 REAL TIME POLYMERASE CHAIN REACTION..................................................................... 58 2.2.17 ELISAS........................................................................................................................ 58 2.2.16 STATISTICAL ANALYSIS..................................................................................................... 59 3. RESULTS.....................................................................................................................................60 3.1 PRESENCE OF IL-6 SIGNALLING-RELATED MOLECULES DURING FRACTURE HEALING.....................60 3.1.1 CHARACTERIZATION OF IMMUNE CELLS IN THE FRACTURE HEMATOMA ............................... 60 3.1.2 CHARACTERIZATION OF THE DEVELOPING FRACTURE CALLUS ................................................ 61 3.2 INHIBITION OF IL-6 SIGNALLING DURING FRACTURE HEALING ........................................................ 62 3.2.1 PRELIMINARY EXPERIMENTS................................................................................................. 62 3.2.2 EFFECTS OF IL-6 INHIBITION DURING THE INFLAMMATORY PHASE............................................65 3.2.3 EFFECTS OF IL-6 INHIBITION DURING THE BONE REPAIR PHASE .............................................. 74 3.3 INFLUENCE OF IL-6 ON OSTEOGENIC AND OSTEOCLASTIC DIFFERENTIATION IN VITRO ................... 76 4. DISCUSSION.............................................................................................................................. 83 4.1 IL-6 SIGNALLING DURING THE TIME COURSE OF BONE FRACTURE HEALING ................................... 64 4.2 ROLE OF IL-6 CLASSIC AND TRANS-SIGNALLING IN THE INFLAMMATORY PHASE...........................86 4.3 ROLE OF IL-6 CLASSIC AND TRANS-SIGNALLING IN THE BONE REPAIR PHASE ............................. 94 5. SUMMARY................................................................................................................................ 98 6. REFERENCES............................................................................................................................100 STATUTORY DECLARATION....................................................................................................................115 ACKNOWLEDGEMENTS...................................................................................................................... 116 CURRICULUM VITAE...........................................................................................................................118
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spelling	Prystaz, Katja 1988- Verfasser (DE-588)1162067055 aut Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Katja Prystaz Ulm [2018] 118 Blätter Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2018 Interleukin 6 (DE-588)4246734-2 gnd rswk-swf Knochenbruch (DE-588)4031318-9 gnd rswk-swf Osteoblast (DE-588)4275206-1 gnd rswk-swf Frakturheilung (DE-588)4209626-1 gnd rswk-swf Fractures, Bone. Fracture healing. Osteoblasts. Osteoclasts. Interleukin-6. (DE-588)4113937-9 Hochschulschrift gnd-content Knochenbruch (DE-588)4031318-9 s Frakturheilung (DE-588)4209626-1 s Osteoblast (DE-588)4275206-1 s Interleukin 6 (DE-588)4246734-2 s DE-604 Universität Ulm (DE-588)30607-1 dgg B:DE-101 application/pdf http://d-nb.info/1162099135/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030612749&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Prystaz, Katja 1988- Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Interleukin 6 (DE-588)4246734-2 gnd Knochenbruch (DE-588)4031318-9 gnd Osteoblast (DE-588)4275206-1 gnd Frakturheilung (DE-588)4209626-1 gnd
subject_GND	(DE-588)4246734-2 (DE-588)4031318-9 (DE-588)4275206-1 (DE-588)4209626-1 (DE-588)4113937-9
title	Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing
title_auth	Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing
title_exact_search	Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing
title_full	Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Katja Prystaz
title_fullStr	Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Katja Prystaz
title_full_unstemmed	Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Katja Prystaz
title_short	Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing
title_sort	role of interleukin 6 in the inflammatory and bone formation phase during fracture healing
topic	Interleukin 6 (DE-588)4246734-2 gnd Knochenbruch (DE-588)4031318-9 gnd Osteoblast (DE-588)4275206-1 gnd Frakturheilung (DE-588)4209626-1 gnd
topic_facet	Interleukin 6 Knochenbruch Osteoblast Frakturheilung Hochschulschrift
url	http://d-nb.info/1162099135/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030612749&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT prystazkatja roleofinterleukin6intheinflammatoryandboneformationphaseduringfracturehealing AT universitatulm roleofinterleukin6intheinflammatoryandboneformationphaseduringfracturehealing

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