Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Ulm
[2018]
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 118 Blätter Illustrationen, Diagramme 30 cm |
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| 245 | 1 | 0 | |a Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing |c Katja Prystaz |
| 264 | 1 | |a Ulm |c [2018] | |
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| 653 | |a Fractures, Bone. | ||
| 653 | |a Fracture healing. | ||
| 653 | |a Osteoblasts. | ||
| 653 | |a Osteoclasts. | ||
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
1.
INTRODUCTION................................................................................................................................9
1.1 BONE
METABOLISM...................................................................................................................
9
1.1.1 THE OSTEOBLASTS AND OSTEOCLASTS
..............................................................................
10
1.2 FRACTURE
HEALING...................................................................................................................
12
1.2.1 INFLAMMATORY
PHASE......................................................................................................12
1.2.2 REPAIR
PHASE.................................................................................................................13
1.2.3 REMODELLING
PHASE.......................................................................................................13
1.3 IL-6
SIGNALLING........................................................................................................................14
1.3.1
IL-6..................................................................................................................................
14
1.3.2 IL-6 CLASSIC AND
TRANS-SIGNALLING.................................................................................16
1.3.3 INTRACELLULAR SIGNALLING OF IL-6
.....................................................................................
17
1.3.4 PRO- AND ANTI-INFLAMMATORY EFFECTS OF
IL-6................................................................19
1.3.5 BLOCKING STRATEGIES OF IL-6 SIGNALLING
.......................................................................
21
1.4 IL-6 AND BONE
METABOLISM..................................................................................................25
1.4.1 BONE PHENOTYPES OF GENETICALLY MODIFIED MICE
......................................................
25
1.4.2 INFLUENCE OF IL-6 ON OSTEOBLASTS
................................................................................
27
1.4.3 INFLUENCE OF IL-6 ON OSTEOCLASTS
................................................................................
27
1.5 IL-6 AND FRACTURE
HEALING....................................................................................................26
1.6 AIM OF THE
STUDY...................................................................................................................29
2. MATERIALS AND
METHODS..........................................................................................................30
2.1
MATERIALS...............................................................................................................................
30
2.1.1
ANIMALS..........................................................................................................................30
2.1.2
CONSUMABLES...............................................................................................................
30
2.1.3
CHEMICALS.....................................................................................................................31
2.1.4 BUFFERS AND
SOLUTIONS...................................................................................................33
2.1.5 CELL LINES, MEDIA AND REAGENTS FOR CELL CULTURE
.......................................................
36
2.1.6 ANTIBODIES AND
CONJUGATES........................................................................................
38
2.1.7
PRIMERS..........................................................................................................................
40
2.1.8 KITS
...................................................................
,...........................................................40
2.1.9
EQUIPMENT.....................................................................................................................41
2.1.10
SOFTWARE......................................................................................................................43
2.2
METHODS................................................................................................................................
43
2.2.1 ANIMAL HOUSING AND STUDY
DESIGN.............................................................................43
2.2.2 ANALGESIA, ANAESTHESIA AND PREOPERATIVE
PROCEDURE............................................44
2.2.3 FEMUR
OSTEOTOMY........................................................................................................
45
2.2.4 EUTHANASIA AND SAMPLE
COLLECTION............................................................................46
2.2.5 LIVER
HOMOGENATES.....................................................................................................46
2.2.6 MULTIPLEX ANALYSIS OF PLASMA AND HEMATOMA HOMOGENATES
...............................
47
2.2.7 WESTERN
BLOTS..............................................................................................................
47
2.2.8 FLUORESCENT-ACTIVATED CELL SORTING (FACS)-ANALYSIS
...............................................
48
2.2.9
HISTOLOGY.......................................................................................................................
49
2.2.10 BIOMECHANICAL TESTING OF
FEMURS.............................................................................
52
2.2.11 MICRO-COMPUTED TOMOGRAPHY OF
FEMURS.................................................................53
2.2.12 PRIMARY CELL ISOLATION AND CELL CULTURE MAINTENANCE
.............................................
54
2.2.13 GENE AND PROTEIN ANALYSES OF IL-6-STIMULATED RAW264.7 CELLS
......................
54
2.2.14 GENE AND PROTEIN ANALYSES OF IL-6-STIMULATED OSTEOBLASTS
................................
56
2.2.15 IMMUNOFLUORESCENCE
STAINING..................................................................................57
2.2.16 REAL TIME POLYMERASE CHAIN
REACTION.....................................................................
58
2.2.17
ELISAS........................................................................................................................
58
2.2.16 STATISTICAL
ANALYSIS.....................................................................................................
59
3.
RESULTS.....................................................................................................................................60
3.1 PRESENCE OF IL-6 SIGNALLING-RELATED MOLECULES DURING FRACTURE
HEALING.....................60
3.1.1 CHARACTERIZATION OF IMMUNE CELLS IN THE FRACTURE HEMATOMA
...............................
60
3.1.2 CHARACTERIZATION OF THE DEVELOPING FRACTURE CALLUS
................................................
61
3.2 INHIBITION OF IL-6 SIGNALLING DURING FRACTURE HEALING
........................................................
62
3.2.1 PRELIMINARY
EXPERIMENTS.................................................................................................
62
3.2.2 EFFECTS OF IL-6 INHIBITION DURING THE INFLAMMATORY
PHASE............................................65
3.2.3 EFFECTS OF IL-6 INHIBITION DURING THE BONE REPAIR PHASE
..............................................
74
3.3 INFLUENCE OF IL-6 ON OSTEOGENIC AND OSTEOCLASTIC DIFFERENTIATION IN
VITRO
...................
76
4.
DISCUSSION..............................................................................................................................
83
4.1 IL-6 SIGNALLING DURING THE TIME COURSE OF BONE FRACTURE HEALING
...................................
64
4.2 ROLE OF IL-6 CLASSIC AND TRANS-SIGNALLING IN THE INFLAMMATORY
PHASE...........................86
4.3 ROLE OF IL-6 CLASSIC AND TRANS-SIGNALLING IN THE BONE REPAIR PHASE
.............................
94
5.
SUMMARY................................................................................................................................
98
6.
REFERENCES............................................................................................................................100
STATUTORY
DECLARATION....................................................................................................................115
ACKNOWLEDGEMENTS......................................................................................................................
116
CURRICULUM
VITAE...........................................................................................................................118
|
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| author | Prystaz, Katja 1988- |
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| discipline | Biologie |
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| spelling | Prystaz, Katja 1988- Verfasser (DE-588)1162067055 aut Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Katja Prystaz Ulm [2018] 118 Blätter Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2018 Interleukin 6 (DE-588)4246734-2 gnd rswk-swf Knochenbruch (DE-588)4031318-9 gnd rswk-swf Osteoblast (DE-588)4275206-1 gnd rswk-swf Frakturheilung (DE-588)4209626-1 gnd rswk-swf Fractures, Bone. Fracture healing. Osteoblasts. Osteoclasts. Interleukin-6. (DE-588)4113937-9 Hochschulschrift gnd-content Knochenbruch (DE-588)4031318-9 s Frakturheilung (DE-588)4209626-1 s Osteoblast (DE-588)4275206-1 s Interleukin 6 (DE-588)4246734-2 s DE-604 Universität Ulm (DE-588)30607-1 dgg B:DE-101 application/pdf http://d-nb.info/1162099135/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030612749&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Prystaz, Katja 1988- Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Interleukin 6 (DE-588)4246734-2 gnd Knochenbruch (DE-588)4031318-9 gnd Osteoblast (DE-588)4275206-1 gnd Frakturheilung (DE-588)4209626-1 gnd |
| subject_GND | (DE-588)4246734-2 (DE-588)4031318-9 (DE-588)4275206-1 (DE-588)4209626-1 (DE-588)4113937-9 |
| title | Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing |
| title_auth | Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing |
| title_exact_search | Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing |
| title_full | Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Katja Prystaz |
| title_fullStr | Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Katja Prystaz |
| title_full_unstemmed | Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing Katja Prystaz |
| title_short | Role of Interleukin-6 in the inflammatory and bone formation phase during fracture healing |
| title_sort | role of interleukin 6 in the inflammatory and bone formation phase during fracture healing |
| topic | Interleukin 6 (DE-588)4246734-2 gnd Knochenbruch (DE-588)4031318-9 gnd Osteoblast (DE-588)4275206-1 gnd Frakturheilung (DE-588)4209626-1 gnd |
| topic_facet | Interleukin 6 Knochenbruch Osteoblast Frakturheilung Hochschulschrift |
| url | http://d-nb.info/1162099135/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030612749&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT prystazkatja roleofinterleukin6intheinflammatoryandboneformationphaseduringfracturehealing AT universitatulm roleofinterleukin6intheinflammatoryandboneformationphaseduringfracturehealing |
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