Structuresor Why things don’t fall down:
I am very much aware that it is an act of extreme rashness to attempt to write an elementary book about structures. Indeed it is only when the subject is stripped of its mathematics that one begins to realize how difficult it is to pin down and describe those structural concepts which are often call...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Boston, MA
Springer US
1978
|
| Schlagworte: | |
| Online-Zugang: | BTU01 Volltext Inhaltsverzeichnis |
| Zusammenfassung: | I am very much aware that it is an act of extreme rashness to attempt to write an elementary book about structures. Indeed it is only when the subject is stripped of its mathematics that one begins to realize how difficult it is to pin down and describe those structural concepts which are often called' elementary'; by which I suppose we mean 'basic' or 'fundamental'. Some of the omis sions and oversimplifications are intentional but no doubt some of them are due to my own brute ignorance and lack of under standing of the subject. Although this volume is more or less a sequel to The New Science of Strong Materials it can be read as an entirely separate book in its own right. For this reason a certain amount of repetition has been unavoidable in the earlier chapters. I have to thank a great many people for factual information, suggestions and for stimulating and sometimes heated discussions. Among the living, my colleagues at Reading University have been generous with help, notably Professor W. D. Biggs (Professor of Building Technology), Dr Richard Chaplin, Dr Giorgio Jeronimidis, Dr Julian Vincent and Dr Henry Blyth; Professor Anthony Flew, Professor of Philosophy, made useful suggestions about the last chapter. I am also grateful to Mr John Bartlett, Consultant Neurosurgeon at the Brook Hospital. Professor T. P. Hughes of the University of the West Indies has been helpful about rockets and many other things besides. My secretary, Mrs Jean Collins, was a great help in times of trouble. Mrs Nethercot of Vogue was kind to me about dressmaking. Mr Gerald Leach and also many of the editorial staff of Penguins have exercised their accustomed patience and helpfulness. Among the dead, l owe a great deal to Dr Mark Pryor - lately of Trinity College, Cambridge - especially for discussions about biomechanics which extended over a period of nearly thirty years. Lastly, for reasons which must surely be obvious, l owe a humble oblation to Herodotus, once a citizen of Halicamassus |
| Beschreibung: | 1 Online-Ressource (395 p) |
| ISBN: | 9781461590743 |
| DOI: | 10.1007/978-1-4615-9074-3 |
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| 520 | |a Among the living, my colleagues at Reading University have been generous with help, notably Professor W. D. Biggs (Professor of Building Technology), Dr Richard Chaplin, Dr Giorgio Jeronimidis, Dr Julian Vincent and Dr Henry Blyth; Professor Anthony Flew, Professor of Philosophy, made useful suggestions about the last chapter. I am also grateful to Mr John Bartlett, Consultant Neurosurgeon at the Brook Hospital. Professor T. P. Hughes of the University of the West Indies has been helpful about rockets and many other things besides. My secretary, Mrs Jean Collins, was a great help in times of trouble. Mrs Nethercot of Vogue was kind to me about dressmaking. Mr Gerald Leach and also many of the editorial staff of Penguins have exercised their accustomed patience and helpfulness. Among the dead, l owe a great deal to Dr Mark Pryor - lately of Trinity College, Cambridge - especially for discussions about biomechanics which extended over a period of nearly thirty years. | ||
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Datensatz im Suchindex
| _version_ | 1804178875586969600 |
|---|---|
| adam_text | CHARAKTERISIERUNG DER
RENINZELLREKRUTIERUNG IN DER
ALDOSTERONSYNTHASE-KNOCKOUT-MAUS
DISSERTATION
ZUR ERLANGUNG DES DOKTORGRADES DER
MEDIZIN
DER FAKULTAET FUER BIOLOGIE UND VORKLINISCHE MEDIZIN
DER UNIVERSITAET REGENSBURG
VORGELEGT VON
CATHARINA KESSLER
AUS ERLANGEN
IM JAHR 2014
HTTP://D-NB.INFO/1053828985
INHALTSVERZEICHNIS
1. ZUSAMMENFASSUNG 5
2. EINLEITUNG 8
2.1.
DAS RENIN-ANGIOTENSIN-ALDOSTERON-SYSTEM (RAAS) 9
2.1.1.
RENIN 10
2.1.1.1. RENINSYNTHESE IN DER JUXTAGLOMERULAEREN ZELLE 11
2.1.1.2. DIE REGULATION DES RENINSYSTEMS 12
2.1.2. ALDOSTERON 13
2.1.2.1. ALDOSTERONWIRKUNG 14
2.1.2.2.
MINERALOCORTICOID REZEPTOR (MR) 15
2.1.2.3. ALDOSTERONSYNTHASE 16
2.1.2.4. ALDOSTERONSYNTHASE-KNOCKOUT-MAUS 17
2.2.
GEFAESSSYSTEM DER MAUSNIERE 19
2.3. ZIELSETZUNG DER ARBEIT 20
3. MATERIAL UND METHODEN 22
3.1.
MATERIAL 23
3.1.1.
GERAETE 23
3.1.2. VERBRAUCHSMATERIAL 24
3.1.3.
CHEMIKALIEN 25
3.1.4. ANTIKOERPER 26
3.1.5.
PUFFER UND LOESUNGEN 27
3.1.5.1.
IMMUNHISTOCHEMIE 27
3.1.5.2.
MOLEKULARBIOLOGIE 28
3.1.6. SOFTWARE UND INTERNETDIENSTE 28
3.2.
METHODEN 29
3.2.1. VERSUCHSTIERE 29
3.2.2.
GENOTYPISIERUNG 29
3.2.3. HISTOLOGISCHE METHODEN 31
3.2.3.1. FIXIERUNG DES NIERENGEWEBES 31
3.2.3.2.
EINBETTUNG IN PARAFFIN 32
3.2.3.3. ANFERTIGEN VON PARAFFINSCHNITTEN UND ENTPARAFFINIERUNG 32
3.2.3.4. IMMUNHISTOCHEMISCHE FAERBUNG 33
3.2.4.
DREIDIMENSIONALE REKONSTRUKTION 34
3.2.4.1.
MIKROSKOPIE UND DIGITALISIERUNG 34
3
3.2.4.2.
DATENAUFBEREITUNG MITTELS IMAGEJ 35
3.2.4.3.
REKONSTRUKTION MITTELS AMIRA 36
3.2.5.
MOLEKULARBIOLOGISCHE METHODEN 38
3.2.5.1.
ISOLIERUNG VON TOTAL-RNA AUS NIEREN 38
3.2.5.2.
REVERSE TRANSKRIPTION (CDNA-SYNTHESE) 39
3.2.5.3.
QUANTITATIVE POLYMERASEKETTENREAKTION (REAL-TIME-PCR) 40
4. ERGEBNISSE 42
4.1.3D-REKONSTRUKTION UND ZELLULAERE ANALYSE 44
4.1.1.
WILDTYP BEI NORMALSALZDIAET (WT NS) 44
4.1.2.
WILDTYP NACH SALZDEPLETION IN KOMBINATION MIT ENALAPRIL FUER
EINE WOCHE (WT LS/E 1 WO) 47
4.1.3.
WILDTYP NACH SALZDEPLETION IN KOMBINATION MIT ENALAPRIL FUER
DREI WOCHEN (WT LS/E 3 WO) 50
4.1.4. ALDOSTERONSYNTHASE-KNOCKOUT-MAUS BEI NORMALSALZDIAET
(KO NS) 54
4.1.5. ALDOSTERONSYNTHASE-KNOCKOUT-MAUS NACH ZEHN TAGEN
SALZDEPLETION (KO LS 10 D) 59
4.2.
MRNA UND VOLUMINA 64
5. DISKUSSION 68
5.1.
RENINEXPRESSIONSMUSTER IN DER MAUSNIERE NACH SALZDEPLETION UND
VERABREICHUNG EINES ACE-HEMMERS 69
5.2.
RAAS-DEFEKTMUTANTE MIT GENETISCHER DELETION DER
ALDOSTERONSYNTHESE 70
6. LITERATURVERZEICHNIS 75
7. ANHANG 83
7.1.
ABBILDUNGSVERZEICHNIS 84
7.2. ABKUERZUNGSVERZEICHNIS 86
7.3.
DANKSAGUNG 88
7.4.
ERKLAERUNG 89
4
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| spelling | Gordon, J. E. Verfasser aut Structuresor Why things don’t fall down by J. E. Gordon Boston, MA Springer US 1978 1 Online-Ressource (395 p) txt rdacontent c rdamedia cr rdacarrier I am very much aware that it is an act of extreme rashness to attempt to write an elementary book about structures. Indeed it is only when the subject is stripped of its mathematics that one begins to realize how difficult it is to pin down and describe those structural concepts which are often called' elementary'; by which I suppose we mean 'basic' or 'fundamental'. Some of the omis sions and oversimplifications are intentional but no doubt some of them are due to my own brute ignorance and lack of under standing of the subject. Although this volume is more or less a sequel to The New Science of Strong Materials it can be read as an entirely separate book in its own right. For this reason a certain amount of repetition has been unavoidable in the earlier chapters. I have to thank a great many people for factual information, suggestions and for stimulating and sometimes heated discussions. Among the living, my colleagues at Reading University have been generous with help, notably Professor W. D. Biggs (Professor of Building Technology), Dr Richard Chaplin, Dr Giorgio Jeronimidis, Dr Julian Vincent and Dr Henry Blyth; Professor Anthony Flew, Professor of Philosophy, made useful suggestions about the last chapter. I am also grateful to Mr John Bartlett, Consultant Neurosurgeon at the Brook Hospital. Professor T. P. Hughes of the University of the West Indies has been helpful about rockets and many other things besides. My secretary, Mrs Jean Collins, was a great help in times of trouble. Mrs Nethercot of Vogue was kind to me about dressmaking. Mr Gerald Leach and also many of the editorial staff of Penguins have exercised their accustomed patience and helpfulness. Among the dead, l owe a great deal to Dr Mark Pryor - lately of Trinity College, Cambridge - especially for discussions about biomechanics which extended over a period of nearly thirty years. Lastly, for reasons which must surely be obvious, l owe a humble oblation to Herodotus, once a citizen of Halicamassus Engineering Civil Engineering Civil engineering Baukonstruktion (DE-588)4004821-4 gnd rswk-swf Statik (DE-588)4056987-1 gnd rswk-swf Statik (DE-588)4056987-1 s 1\p DE-604 Baukonstruktion (DE-588)4004821-4 s 2\p DE-604 Erscheint auch als Druck-Ausgabe 9781461590767 https://doi.org/10.1007/978-1-4615-9074-3 Verlag URL des Erstveröffentlichers Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030574726&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Gordon, J. E. Structuresor Why things don’t fall down Engineering Civil Engineering Civil engineering Baukonstruktion (DE-588)4004821-4 gnd Statik (DE-588)4056987-1 gnd |
| subject_GND | (DE-588)4004821-4 (DE-588)4056987-1 |
| title | Structuresor Why things don’t fall down |
| title_auth | Structuresor Why things don’t fall down |
| title_exact_search | Structuresor Why things don’t fall down |
| title_full | Structuresor Why things don’t fall down by J. E. Gordon |
| title_fullStr | Structuresor Why things don’t fall down by J. E. Gordon |
| title_full_unstemmed | Structuresor Why things don’t fall down by J. E. Gordon |
| title_short | Structuresor Why things don’t fall down |
| title_sort | structuresor why things don t fall down |
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