Mass Spectrometry and Genomic Analysis:
76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Se...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Dordrecht
Springer Netherlands
2001
|
| Schriftenreihe: | Focus on Structural Biology
2 |
| Schlagworte: | |
| Online-Zugang: | UBT01 Volltext |
| Zusammenfassung: | 76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Müller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein Separation 95 Protein Identification Using Peptide Mass Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2. 2. 2. MS/MS Analysis 102 Improvement of the Identification by Chemical Modification of Peptides 106 2. 2. 3 |
| Beschreibung: | 1 Online-Ressource (XV, 149 p) |
| ISBN: | 9780306475955 |
| DOI: | 10.1007/0-306-47595-2 |
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| indexdate | 2024-07-10T08:10:06Z |
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| spelling | Mass Spectrometry and Genomic Analysis edited by J. Nicholas Housby Dordrecht Springer Netherlands 2001 1 Online-Ressource (XV, 149 p) txt rdacontent c rdamedia cr rdacarrier Focus on Structural Biology 2 76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Müller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein Separation 95 Protein Identification Using Peptide Mass Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2. 2. 2. MS/MS Analysis 102 Improvement of the Identification by Chemical Modification of Peptides 106 2. 2. 3 Chemistry Mass Spectrometry Life Sciences, general Analytical Chemistry Human Genetics Animal Anatomy / Morphology / Histology Biochemistry, general Human genetics Analytical chemistry Mass spectrometry Life sciences Biochemistry Animal anatomy Nicholas Housby, J. edt Erscheint auch als Druck-Ausgabe 9780792371731 https://doi.org/10.1007/0-306-47595-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Mass Spectrometry and Genomic Analysis Chemistry Mass Spectrometry Life Sciences, general Analytical Chemistry Human Genetics Animal Anatomy / Morphology / Histology Biochemistry, general Human genetics Analytical chemistry Mass spectrometry Life sciences Biochemistry Animal anatomy |
| title | Mass Spectrometry and Genomic Analysis |
| title_auth | Mass Spectrometry and Genomic Analysis |
| title_exact_search | Mass Spectrometry and Genomic Analysis |
| title_full | Mass Spectrometry and Genomic Analysis edited by J. Nicholas Housby |
| title_fullStr | Mass Spectrometry and Genomic Analysis edited by J. Nicholas Housby |
| title_full_unstemmed | Mass Spectrometry and Genomic Analysis edited by J. Nicholas Housby |
| title_short | Mass Spectrometry and Genomic Analysis |
| title_sort | mass spectrometry and genomic analysis |
| topic | Chemistry Mass Spectrometry Life Sciences, general Analytical Chemistry Human Genetics Animal Anatomy / Morphology / Histology Biochemistry, general Human genetics Analytical chemistry Mass spectrometry Life sciences Biochemistry Animal anatomy |
| topic_facet | Chemistry Mass Spectrometry Life Sciences, general Analytical Chemistry Human Genetics Animal Anatomy / Morphology / Histology Biochemistry, general Human genetics Analytical chemistry Mass spectrometry Life sciences Biochemistry Animal anatomy |
| url | https://doi.org/10.1007/0-306-47595-2 |
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