Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands: = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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Köln
[2018]
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | Text englisch. Zusammenfassung in deutscher und englischer Sprache |
| Beschreibung: | 84 Blätter Illustrationen |
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| 245 | 1 | 0 | |a Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands |b = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |c vorgelegt von Ronja Katharina Ocker |
| 246 | 1 | 3 | |a Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |
| 246 | 1 | 1 | |a Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |
| 264 | 1 | |a Köln |c [2018] | |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
GLOSSARY...................................................................................................................................................IV
1
INTRODUCTION.....................................................................................................................................1
1.1 BACKGROUND AND OBSERVATIONS LEADING TO THE RESEARCH QUESTIONS OF
THIS THESIS
.......................
1
1.2 AIM OF THE PRESENT
STUDY............................................................................................................
4
2 LITERATURE
REVIEW.............................................................................................................................
5
2.1 GENERAL DESCRIPTION OF PLASMODIUM, LIFE-CYCLE OF THE PARASITE, AND
MALARIA DISEASE
..............
5
2.2 CURRENT APPROACHES FOR MALARIA
DIAGNOSIS................................................................................7
2.2.1 NESTED POLYMERASE CHAIN REACTION
(NPCR).....................................................................
7
2.2.2 LOOP-MEDIATED ISOTHERMAL AMPLIFICATION
(LAMP)..........................................................8
2.3 CHALLENGES CAUSED BY MALARIA IN
THAILAND..............................................................................12
3 MATERIAL AND
METHODS...................................................................................................................
15
3.1 OVERVIEW ON MATERIAL COLLECTION AND GEOGRAPHY OF THE INVESTIGATED
AREA IN THAILAND
.........
15
3.2 MICROSCOPIC EXAMINATION OF THE
SAMPLES................................................................................17
3.2.1 MICROSCOPY AT THE PARTICIPATING HOSPITALS
.....................................................................
17
3.2.1.1 SLIDE
PREPARATION....................................................................................................17
3.2.1.2
STAINING..................................................................................................................
17
3.2.1.3 SCREENING THE SLIDES AT MICROSCOPE FOR MALARIA
DIAGNOSIS....................................18
3.2.2 MICROSCOPY AT REGIONAL MEDICAL SCIENCES CENTER 3
.....................................................
18
3.3 DNA EXTRACTION FOR THE IDENTIFICATION OF PLASMODIAL INFECTIONS BY
PCR AND LAMP...........18
3.4 NESTED PCR ASSAY BASED ON 188 RRNA
GENE..........................................................................19
3.4.1 PREPARATION OF THE PRIMERS FOR
PLASMODIUM DNA DETECTION
......................................
20
3.5 LAMP ASSAY BASED ON 188 RRNA
PLASMODIUM
GENE............................................................21
3.5.1 LAMP PRIMER
SETS.........................................................................................................
22
3.5.2 PREPARATION OF LAMP REACTION BUFFER
...........................................................................
24
3.6 VISUALIZATION OF THE AMPLIFIED PRODUCTS BY GEL ELECTROPHORESIS
............................................
25
3.7 STATISTICAL METHODS
26
4 RESULTS
28
4.1 MICROSCOPIC DETECTION OF PLASMODIUM
SPP............................................................................28
4.2 DNA BASED
DETECTION..............................................................................................................
28
4.2.1 NESTED PC R
....................................................................................................................28
4.2.2 LAMP ASSAYS FOR DNA -PLASMODIUM SPP. DETECTION
.................................................
29
4.2.3 PRE-TRIALS ON LAMP REACTION BUFFER
..............................................................................
30
4.2.3.1 OPTIMIZATION OF LAMP BUFFER DILUTION
................................................................
30
4.2.3.2 EXPERIMENTS WITH DIFFERENT DNTP STOCK CONCENTRATIONS
.....................................
30
4.2.4 PRE-TRIALS FOR THE SPECIES- SPECIFIC LAMP
ASSAYS.........................................................31
4.2.4.1 ESTABLISHING A LAMP ASSAY FOR P FALCIPARUM
..................................................... 31
4.2.4.2 EXPERIMENTS IN ORDER TO ADOPT THE LAMP ASSAY FOR
P VIVAX
..............................
32
4.3 STATISTICAL
ANALYSIS....................................................................................................................33
4.3.1 OVERALL OBSERVED FREQUENCY OF PLASMODIUM SPECIES INFECTIONS
...................................
33
4.3.2 ANALYSIS OF DIAGNOSTIC METHOD SENSITIVITY, SPECIFICITY, NPV AND
PPV.........................34
5
DISCUSSION.....................................................................................................................................
37
5.1 WHY LAMP FOR MALARIA DIAGNOSIS IN
THAILAND?.....................................................................37
5.2 COMPARISON OF MALARIA DIAGNOSTIC TOOLS IN THIS
STUDY............................................................38
5.3 INSIGHTS GAINED THROUGH THIS
STUDY..........................................................................................39
5.4 REVISIONS APPLIED TO THE LAMP ASSAY IN OUR
STUDY...............................................................40
5.5 LAMP FOR
PLASMODIUM DETECTION: PREVIOUS
STUDIES..............................................................41
5.6
CONCLUSIONS..............................................................................................................................47
6
SUMMARY.......................................................................................................................................
49
7
ZUSAMMENFASSUNG........................................................................................................................51
8
REFERENCES....................................................................................................................................
53
9 PRELIMINARY
PUBLICATION...............................................................................................................
66
10
APPENDIX......................................................................................................................................
67
10.1 LIST OF FIGURES
........................................
67
10.2 LIST OF
TABLES.......................................................................................................................
68
11
LEBENSLAUF...................................................................................................................................
84
|
| any_adam_object | 1 |
| author | Ocker, Ronja Katharina 1984- |
| author_GND | (DE-588)1157093426 |
| author_facet | Ocker, Ronja Katharina 1984- |
| author_role | aut |
| author_sort | Ocker, Ronja Katharina 1984- |
| author_variant | r k o rk rko |
| building | Verbundindex |
| bvnumber | BV045145468 |
| ctrlnum | (OCoLC)1136403316 (DE-599)DNB1157128416 |
| discipline | Medizin |
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| spelling | Ocker, Ronja Katharina 1984- Verfasser (DE-588)1157093426 aut Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand vorgelegt von Ronja Katharina Ocker Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand Köln [2018] 84 Blätter Illustrationen txt rdacontent n rdamedia nc rdacarrier Text englisch. Zusammenfassung in deutscher und englischer Sprache Dissertation Universität zu Köln 2017 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1157128416/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030535227&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ocker, Ronja Katharina 1984- Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |
| subject_GND | (DE-588)4113937-9 |
| title | Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |
| title_alt | Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |
| title_auth | Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |
| title_exact_search | Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |
| title_full | Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand vorgelegt von Ronja Katharina Ocker |
| title_fullStr | Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand vorgelegt von Ronja Katharina Ocker |
| title_full_unstemmed | Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand vorgelegt von Ronja Katharina Ocker |
| title_short | Anwendung der LAMP (Loop-Mediated Isothermal Amplification) für die Diagnose von Malaria in klinischen Proben aus zentralen und westlichen Provinzen Thailands |
| title_sort | anwendung der lamp loop mediated isothermal amplification fur die diagnose von malaria in klinischen proben aus zentralen und westlichen provinzen thailands application of lamp loop mediated isothermal amplification for the diagnosis of malaria infections in clinical samples from central and western provinces of thailand |
| title_sub | = Application of LAMP (Loop-Mediated Isothermal Amplification) for the diagnosis of malaria infections in clinical samples from central and western provinces of Thailand |
| topic_facet | Hochschulschrift |
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