Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Erlangen ; Nürnberg
[2018]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 138 Seiten Illustrationen, Diagramme 21 cm |
Internformat
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| 245 | 1 | 0 | |a Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression |c vorgelegt von Julia Freiin von Wittgenstein |
| 264 | 1 | |a Erlangen ; Nürnberg |c [2018] | |
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENT
DANKSAGUNG............................................................................................................................................3
ABSTRACT....................................................................................................................................................5
ZUSAMMENFASSUNG...............................................................................................................................
7
FIGURES...................................................................................................................................................12
TABLES.....................................................................................................................................................
12
ABBREVIATIONS......................................................................................................................................
13
INTRODUCTION..........................................................................................................................................17
I. FUNDAMENTAL ORGANIZATION OF MEMORY
..................................................................................
17
II. CELLULAR CORRELATES OF MEMORY
STORAGE.................................................................................
18
I. SYNAPTIC
PLASTICITY...................................................................................................................18
II. I NTRINSIC
PLASTICITY....................................................................................................................20
III. MOLECULAR PATHWAYS UNDERLYING NEURONAL
PLASTICITY........................................................22
I. POSTSYNAPTIC L T P
.................................................................................................................
22
II. PRESYNAPTIC
LTP...................................................................................................................
24
III. INTRINSIC
PLASTICITY..................................................................................................................
25
IV. CYTOSKELETON AND SYNAPTIC
PLASTICITY.................................................................................27
V. ACTIVITY-DEPENDENT GENE
TRANSCRIPTION...............................................................................29
IV. THE HIPPOCAMPAL NETWORK AND REGION-SPECIFIC PLASTICITY
..............................................
32
V. THE FUNCTION OF SOX11 IN THE
BRAIN.......................................................................................35
VI. AIM OF THE
STUDY....................................................................................................................37
R
ESULTS...................................................................................................................................................38
I. THE SOX11 LOCUS SHOWS TRAITS OF A TISSUE-SPECIFIC ACTIVITY-DEPENDENT
GENE
...................
38
II. SOX11 IS INDUCED BY HIPPOCAMPAL
ACTIVITY..........................................................................40
I. ECS TREATMENT ENHANCES SOX11 EXPRESSION IN THE D G
..............................................
41
II. EXPOSURE TO ENRICHED ENVIRONMENT INDUCES SOX11 IN MATURE DG GCS
....................
42
III. SOX11 IS EXPRESSED BY ACTIVATED DG GRANULE CELLS
......................................................
45
IV. INDUCTION OF SOX11 IS REGION-SPECIFIC
..............................................................................
45
III. AAV-MEDIATED OVEREXPRESSION OF SOX11 EFFICIENTLY GENERATES A
GAIN-OF-FUNCTION
MODEL.........................................................................................................................................
47
I. AAV SEROTYPE 9 SHOWS EFFICIENT SPREADING FROM THE INJECTION SITE
..............................
47
II. AAV SEROTYPE 9 TARGETS PREFERENTIALLY MATURE NEURONAL CELLS
......................................
48
III. AAV-MEDIATED OVEREXPRESSION OF SOX11 LEAVES EXPRESSION OF MOST
DIFFERENTIATION
STAGE-SPECIFIC MARKERS IN DG GRANULE CELLS
INTACT..........................................................50
IV. THE ROLE OF SOX11 IN HIPPOCAMPUS-DEPENDENT LEARNING
.............................................
53
I. SOX11-OE DOES NOT IMPAIR THE FORMATION OF CONTEXT MEMORY
.....................................
53
II. SOX11-OE INCREASES STABILITY AGAINST INTERFERENCE WITH RECENT
SPATIAL MEMORY
......
56
V. SOX11 AS MODULATOR OF DG
PLASTICITY....................................................................................59
I. SOX11-OE INCREASES EXCITABILITY OF DG GRANULE CELLS
...................................................
60
II. SOX11-OE INDUCES STRONGER INHIBITORY INPUT AND REDUCED SYNAPTIC
PLASTICITY ON
CIRCUIT
LEVEL.............................................................................................................................
60
III. 80X11 EFFECTS ON DG PLASTICITY ARE INDEPENDENT FROM ADULT
HIPPOCAMPAL
NEUROGENESIS..........................................................................................................................65
VI. MOLECULAR MECHANISMS OF THE 80X11 EFFECTS ON DG PLASTICITY
...................................
67
I. SOX11-OE MODULATES GENES INVOLVED IN NEURONAL MEMBRANE CONDUCTANCE
...........
67
II. 8 0 X 1 1 -OE ALTERS EXPRESSION OF SEMAPHORIN SIGNALING NETWORKS AND
CYTOSKELETAL
COMPONENTS............................................................................................................................70
III. THE MICROTUBULE-REGULATORY PROTEIN STMN1 IS A TARGET GENE OF 8 0 X
1 1
....................
73
IV. EXPRESSION OF PUTATIVE 80X11 TARGET GENES FOLLOWING STIMULATION BY A
PHYSIOLOGICAL
HIPPOCAMPAL
STIMULUS...........................................................................................................76
VII. GENERATION AND VALIDATION OF A LOSS-OF-FUNCTION MOUSE MODEL TO
INVESTIGATE THE ROLE OF
80X11 IN MATURE
NEURONS......................................................................................................
78
DISCUSSION............................................................................................................................................
81
I. SOX11 IS AN ACTIVITY-DEPENDENT G EN
E....................................................................................81
II. MOLECULAR MECHANISMS OF ACTIVITY-DEPENDENT SOX11 INDUCTION
...........................................
84
III. REGULATION OF REGION-SPECIFIC SOX11
EXPRESSION..............................................................86
IV. IDENTIFYING THE FUNCTION OF 80X11 IN MATURE DG NEURONS
.............................................
89
V. ACTIVITY-DEPENDENT SOX11 EXPRESSION AND DEPRESSIVE
DISORDERS......................................93
MATERIALS AND
METHODS......................................................................................................................
95
I.
MATERIALS.....................................................................................................................................
95
I. CHEMICALS AND
REAGENTS....................................................................................................95
II. BUFFERS AND
SOLUTIONS............................................................................................................96
III. ENZYMES AND BUFFERS FOR P C R
S.........................................................................................
99
IV.
ANTIBODIES..............................................................................................................................
99
V. LIST OF PRIMERS AND EMSA OLIGONUCLEOTIDES
..................................................................
100
VI.
VIRUSES..................................................................................................................................101
II. ANIMAL
PROCEDURES..................................................................................................................101
I. ANIMAL HOUSING AND MOUSE
LINES.....................................................................................
101
II. TAMOXIFEN
TREATMENT..........................................................................................................103
III. ELECTROCONVULSIVE STIMULATION
(ECS)...............................................................................
103
IV. ENRICHED ENVIRONMENT (EE) EXPLORATION
.........................................................................
104
V. STEREOTACTIC VIRUS
INJECTIONS..............................................................................................
104
VI. BRDU
INJECTIONS....................................................................................................................
104
VII. CONTEXTUAL FEAR
CONDITIONING.......................................................................................
105
VIII. MORRIS WATER M A Z E
.........................................................................................................106
III.
ELECTROPHYSIOLOGY...............................................................................................................
107
IV. TISSUE PROCESSING AND
ANALYSIS.......................................................................................
108
I. PERFUSION AND PREPARATION OF BRAIN
SECTIONS...................................................................108
II. IMMUNOFLUORESCENCE
STAINING...........................................................................................108
III. IMAGING AND COUNTING PROCEDURES
..................................................................................
108
IV. DISSECTION OF THE D G
..........................................................................................................109
V. RNA ISOLATION FROM DG
TISSUE...........................................................................................109
VI. REVERSE TRANSCRIPTION PCR FOR CDNA SYNTHESIS
...........................................................
110
VII. REAL-TIME QUANTITATIVE PCR (QPCR)
..........................................................................
110
V. CELL
CULTURE...............................................................................................................................
112
I. MAINTENANCE OF HEK293T
CELLS.......................................................................................
112
II. TRANSFECTION OF HEK293T
CELLS........................................................................................
112
V I. ANALYSIS OF PROMOTER
SEQUENCES.......................................................................................112
I. LUCIFERASE
ASSAY..................................................................................................................112
II. ELECTROMOBILITY SHIFT ASSAY
(EMSA)................................................................................
113
VII.
STATISTICS...............................................................................................................................
114
LITERATURE...................................................................................................................................115
|
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| author | Wittgenstein, Julia von |
| author_GND | (DE-588)116304282X |
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| bvnumber | BV045107442 |
| ctrlnum | (OCoLC)1136269917 (DE-599)DNB1163042498 |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Wittgenstein, Julia von Verfasser (DE-588)116304282X aut Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression vorgelegt von Julia Freiin von Wittgenstein Erlangen ; Nürnberg [2018] 138 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2018 Hirnfunktion (DE-588)4159930-5 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Transkriptionsfaktor (DE-588)4303350-7 gnd rswk-swf Gyrus dentatus (DE-588)4390857-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Transkriptionsfaktor (DE-588)4303350-7 s Gyrus dentatus (DE-588)4390857-3 s Genexpression (DE-588)4020136-3 s Hirnfunktion (DE-588)4159930-5 s DE-604 B:DE-101 application/pdf http://d-nb.info/1163042498/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030497865&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wittgenstein, Julia von Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression Hirnfunktion (DE-588)4159930-5 gnd Genexpression (DE-588)4020136-3 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Gyrus dentatus (DE-588)4390857-3 gnd |
| subject_GND | (DE-588)4159930-5 (DE-588)4020136-3 (DE-588)4303350-7 (DE-588)4390857-3 (DE-588)4113937-9 |
| title | Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression |
| title_auth | Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression |
| title_exact_search | Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression |
| title_full | Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression vorgelegt von Julia Freiin von Wittgenstein |
| title_fullStr | Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression vorgelegt von Julia Freiin von Wittgenstein |
| title_full_unstemmed | Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression vorgelegt von Julia Freiin von Wittgenstein |
| title_short | Characterization of sox11 as a novel activity-dependent gene with dentate gyrus-specific expression |
| title_sort | characterization of sox11 as a novel activity dependent gene with dentate gyrus specific expression |
| topic | Hirnfunktion (DE-588)4159930-5 gnd Genexpression (DE-588)4020136-3 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Gyrus dentatus (DE-588)4390857-3 gnd |
| topic_facet | Hirnfunktion Genexpression Transkriptionsfaktor Gyrus dentatus Hochschulschrift |
| url | http://d-nb.info/1163042498/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030497865&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wittgensteinjuliavon characterizationofsox11asanovelactivitydependentgenewithdentategyrusspecificexpression |
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