Verfügbarkeit: Novel regulatory mechanism of catabolite repression in Escherichia coli

Novel regulatory mechanism of catabolite repression in Escherichia coli:

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Bibliographische Detailangaben
1. Verfasser:	Chen, Yu (VerfasserIn)
Format:	Abschlussarbeit Buch
Sprache:	English
Veröffentlicht:	Heidelberg [2017]
Schlagworte:	Hochschulschrift
Online-Zugang:	Inhaltsverzeichnis Inhaltsverzeichnis
Beschreibung:	II, 106 Seiten Illustrationen, Diagramme 30 cm

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adam_text	TABLE OF CONTENTS ZUAMMENFASSUNG..........................................................................................................................1 SUMMARY........................................................................................................................................ 3 1 INTRODUCTION............................................................................................................................... 5 1.1 DIAUXIE AND THE UNDERLING REGULATORY MECHANISM.........................................................................5 1.1.1 FROM DIAUXIE TO GLUCOSE EFFECT TO CATABOLITE REPRESSION AND INDUCER EXCLUSION..................6 1.1.2 CAMP RECEPTOR PROTEIN (CRP) AND GENE EXPRESSION REGULATION............................................8 1.1.3 THE LACTOSE OPERON...............................................................................................................11 1.1.4 THE PHOSPHOTRANSFERASE SYSTEM (PTS) MEDIATES CARBON SOURCE SIGNAL TO CONTROL CAMP LEVEL IN CELLS...................................................................................................................................13 1.1.5 ADENYLATE CYCLASE AND THE CONTROL OF CELLULAR CAMP LEVELS.................................................17 1.1.6 PTS-INDEPENDENT CATABOLITE REPRESSION, CAMP-CRP INDEPENDENT CATABOLITE REPRESSION AND INDUCER EXCLUSION BY NON-PTS SUBSTRATES......................................................................................19 1.2 MACROMOLECULAR ACTIVITIES DEPEND ON ION ENVIRONMENT............................................................. 20 1.3 CHANGES OF INTRACELLULAR ION ENVIRONMENT......................................................................................23 1.4 AIMS OF THIS WORK.............................................................................................................................. 25 2 MATERIALS AND METHODS..................................................................... 27 2.1 GROWTH MEDIUM AND GROWTH CONDITIONS........................................................................................ 27 2.2 STRAIN CONSTRUCTION............................................................................................................................ 27 2.3 RNA SEQUENCING AND DATA ANALYSIS . ................................................................................................ 30 2.4 FRET REPORTS CONSTRUCTION AND FRET ASSAY.....................................................................................31 2.5 K* MEASUREMENTS.............................................................................................................................. 31 2.6 PROMOTER ASSAYS............................................................................................................................... 32 2.7 INTRACELLULAR CAMP MEASUREMENTS................................................................................................. 33 3 RESULTS.......................................................................................................................................35 3.1 GENE EXPRESSION CHANGES IN CATABOLITE REPRESSION................................................................... 35 3.1.1 GENERAL FEATURES OF E. COLI GENOME AND TRANSCRIPTOME....................................................... 35 3.1.2 GENE EXPRESSION CHANGES IN CATABOLITE REPRESSION............................................................. 36 3.1.3 GENE ONTOLOGY (GO) ENRICHMENT......................................................................................... 38 3.1.4 CLASSIFICATION OF DIFFERENTIALLY EXPRESSED GENES ACCORDING TO THEIR FUNCTIONS .................... 39 3.1.5 EXPRESSION OF THE CRP REGULON............................................................................................ 43 3.2ACYAA AND ACYAA CRPSTRAIN..........................................................................................................45 3.2.1 GROWTH OF ACYAA AND ACYAA C/?PSTRAINS WITH DIFFERENT CARBON SOURCES ............................ 45 3.2.2 GENE EXPRESSION IN ACYAA CRPSTRAIN DURING CATABOLITE REPRESSION...................................46 3.3 CRP-BASED FRET REPORTER....................................................................................................... 50 3.3.1 CRP RESPONDS TO CAMP AND MONOVALENT CATIONS IN VITRO ................................................... 51 3.3.2 IN VIVO GLUCOSE RESPONSE...................................................................................................... 55 3.3.3 CAMP RESPONDS TO OTHER CARBON SOURCES............................................................................ 56 3.3.4 IN VIVO CAMP RESPONSE IN DIFFERENT CONDITION SUGGESTS THAT SUCH RESPONSE DEPENDS ON CARBON SOURCES.............................................................................................................................. 59 3.4 CHANGE OF INTRACELLULAR AND EXTRACELLULAR [CAMP]..........................................................................62 3.5 REGULATION OF INTRACELLULAR K+............................................................................................................65 3.6 THE DYNAMIC RANGE OF CRP RESPONSE TO CAMP DEPENDS ON INTRACELLULAR K+ CONCENTRATION .... 66 3.6.1 CAMP-CRP SPECIFIC PROM OTER...............................................................................................66 3.6.2 THE KINETICS OF CAMP-CRP-SPECIFIC PROMOTERS VARIES WITH GROWTH CONDITION .................... 67 3.6.3 CAMP-CRP-SPECIFIC PROMOTERS ARE MORE SENSITIVE IN K+ UPTAKE DEFICIENT STRAIN THAN IN WILD TYPE IN LOW K+ MEDIUM..................................................................................................................68 3.7 THE POSSIBILITY OF INTERCELLULAR SIGNALING BY CAMP ........................................................................ 69 4 DISCUSSION.............................................................................................................................. 71 4.1 GENERAL CHANGES IN TRANSCRIPTION PROFILE BETWEEN E. COLIGROWN IN GLUCOSE OR AKG MEDIUM. 71 4.2 THE TRANSCRIPTOME CHANGES IN A CRP STRAIN ................................................................................. 73 4.3 THE DISCREPANCY BETWEEN INTRACELLULAR CAMP CONCENTRATION AND CRP REGULATION ................... 75 4.4 K* CONCENTRATION IS LIKELY THE UNKNOWN FACTOR IN CRP REGULATION ............................................... 75 4.5 CRP REGULATION UNDER OSMOTIC STRESS AND NON-OSMOTIC STRESS CONDITION ................................... 77 4.6 THE REGULATION OF INTRACELLULAR K* CONCENTRATION IN E. COLI...........................................................78 4.7 THE VARIATION WITHIN HOMEOSTASIS.................................................................................................. 78 4.8 GROWTH AND ITS REGULATORY MECHANISMS......................................................................................... 79 REFERENCE:....................................................................................................................................83 APPENDIX...................................................................................................................................... 95
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spelling	Chen, Yu Verfasser (DE-588)1153923815 aut Novel regulatory mechanism of catabolite repression in Escherichia coli presented by M.Sc. Yu Chen Heidelberg [2017] II, 106 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Ruperto-Carola University of Heidelberg 2017 Mit einer Zusammenfassung in englischer und deutscher Sprache (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1154621960/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030492284&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Chen, Yu Novel regulatory mechanism of catabolite repression in Escherichia coli
subject_GND	(DE-588)4113937-9
title	Novel regulatory mechanism of catabolite repression in Escherichia coli
title_auth	Novel regulatory mechanism of catabolite repression in Escherichia coli
title_exact_search	Novel regulatory mechanism of catabolite repression in Escherichia coli
title_full	Novel regulatory mechanism of catabolite repression in Escherichia coli presented by M.Sc. Yu Chen
title_fullStr	Novel regulatory mechanism of catabolite repression in Escherichia coli presented by M.Sc. Yu Chen
title_full_unstemmed	Novel regulatory mechanism of catabolite repression in Escherichia coli presented by M.Sc. Yu Chen
title_short	Novel regulatory mechanism of catabolite repression in Escherichia coli
title_sort	novel regulatory mechanism of catabolite repression in escherichia coli
topic_facet	Hochschulschrift
url	http://d-nb.info/1154621960/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030492284&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT chenyu novelregulatorymechanismofcataboliterepressioninescherichiacoli

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