Mitosis and meiosis: Part B
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA ; San Diego, CA ; Oxford ; London
Academic Press
2018
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in cell biology
Volume 145 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xvii, 371 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128141427 |
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| 245 | 1 | 0 | |a Mitosis and meiosis |n Part B |c edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) |
| 250 | |a First edition | ||
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| 300 | |a xvii, 371 Seiten |b Illustrationen, Diagramme | ||
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Datensatz im Suchindex
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|---|---|
| adam_text | Contents
Contributors............................................................xi
Preface...............................................................xvii
CHAPTER 1 Mitotic live-cell imaging at different timescales..............i
Anna-Maria Olziersky, Chris A. Smith, Nigel Burroughs,
Andrew D. McAinsh, Patrick Meraldi
1. Introduction..............................................2
2. Automated High-Resolution Kinetochore Tracking............3
3. Monitoring Chromosome Segregation and Spindle
Organization Over Several Hours..........................12
4. Monitoring Mitotic Cell Fate Over Several Days..........19
5. Conclusion..............................................22
Acknowledgments.............................................22
References..................................................23
CHAPTER 2 Correlating light microscopy with serial block face
scanning electron microscopy to study
mitotic spindle architecture.............................29
Nicholas I. Clarke, Stephen J. Royle
1. Introduction.............................................30
2. Correlating Light With SBF-SEM in Mitotic Cells..........31
3. Visualization of Mitotic Spindle Architecture in 3D......36
4. Discussion.......................................... 40
Acknowledgments.............................................41
References..................................................41
CHAPTER 3 Quantification of three-dimensional spindle
architecture.............................................45
Norbert Lindow, Stefanie Redemann, Florian Briinig,
Gunar Fabig, Thomas Miiller-Reichert, Steffen Prohaska
1. Introduction.............................................46
2. Rationale................................................47
3. Methods..................................................47
4. Instrumentation.........................................61
5. Discussion and Outlook..................................61
Acknowledgments.............................................63
References..................................................63
VI
Contents
CHAPTER 4 Quantitative live and super-resolution microscopy
of mitotic chromosomes.................................65
Nike Walther, Jan Ellenberg
1. Introduction..........................................66
2. FCS-Calibrated Confocal Imaging of mEGFP-Tagged
Proteins on Mitotic Chromosomes in Living Cells........67
3. STED Microscopy of Fixed and Immunostained
mEGFP-Tagged Proteins on Mitotic Chromosomes...........76
4. Materials and Instrumentation.........................82
5. Conclusion and Outlook................................87
Acknowledgments...........................................88
References................................................89
CHAPTER 5 Live imaging of cell division in 3D stem-cell
organoid cultures......................................91
Ana C.F. Bolhaqueiro, Richard H. van Jaarsveld, Bas Ponsioen,
René M. Overmeer, Hugo J. Snipped, Geed J.P.L. Kops
1. Introduction..........................................92
2. Conclusion...........................................104
Acknowledgments..........................................104
References...............................................104
CHAPTER 6 Choosing the right microscope to image mitosis
in zebrafish embryos: A practical guide...............107
Iskra Yanakieva, Marija Matejcic, Caren Norden
1. Introduction.........................................108
2. Overview of Three Microscope Systems Well Suited
for Imaging Mitosis During Zebrafish Development......110
3. Determining the Mitotic Index in Zebrafish Tissues Using
an LSCM...............................................112
4. Imaging Intracellular Components During Mitosis in Zebrafish
Tissues With High Temporal Resolution Using SDCM......116
5. Tracking Cells Over Several Cell Cycles for Lineage
Tracing Using a LSFM..................................121
6. Conclusion...........................................125
Acknowledgments......................................... 125
References...............................................125
CHAPTER 7 Advanced microscopy methods for bioimaging of
mitotic microtubules in plants........................129
Petra Vyplelovâ, Miroslav Ovecka, George Komis, Jozef Samaj
1. Introduction..........................................130
Contents
2. Preparation of Molecular Markers for the Visualization
of Mitotic Microtubule Arrays...........................139
3. Use of Arabidopsis Mutants to Dissect Mitotic Progression
in Plants...............................................139
4. Materials..............................................140
5. Conclusions............................................151
Acknowledgments........................................... 152
References.................................................152
CHAPTER 8 Microsurgery and microinjection techniques
in mitosis research.................................... 159
Charles A. Day, Jessica Hornick, Alyssa Langfald,
Christopher Mader, Edward H. Hinchcliffe
1. Introduction............................................160
2. Cell Culture......................................... 160
3. Imaging/Manipulation Chambers..........................161
4. Manipulation/Injection Microscope Setup................162
5. Manipulator and Injector...............................163
6. Needles................................................163
7. Imaging Microscope.................................... 165
8. Microinjection Protocol................................166
9. Micromanipulation Protocol.............................168
Acknowledgments............................................170
References.................................................170
CHAPTER 9 Analyzing the micromechanics of the cell division
apparatus...............................................173
Yuta Shimamoto, Tarun M. Kapoor
1. Introduction............................................174
2. Optical Trap-Based “Mini-Spindle” Assay................175
3. Microneedle-Based “Whole-Spindle” Assay................181
Acknowledgments............................................188
References.................................................188
CHAPTER 10 Optogenetic reversible knocksideways, laser
ablation, and photoactivation on the mitotic spindle
in human cells..........................................191
Ana Milas, Mihaela Jagric, Jelena Martincic, Iva M. Tolic
1. Introduction............................................192
2. Optogenetic Reversible Knocksideways...................195
3. Construction of Plasmids for Optogenetic Experiments...196
4. Sample Preparation.....................................197
5. Imaging................................................198
Contents
6. Optogenetic Reversible Knocksideways of TACC3
in Metaphase..........................................199
7. Optogenetic Reversible Knocksideways of KIF18A
in Metaphase..........................................203
8. Combination of Different Optical Approaches for Studying
Mitotic Spindle Dynamics..............................204
9. Conclusion............................................209
Acknowledgments......................................... 210
References...............................................210
CHAPTER 11 Live imaging of C. elegans oocytes and early
embryos...............................................217
Kimberley Laband, Benjamin Lacroix, Frances Edwards,
Julie C. Canman, Julien Dumont
1. Introduction..........................................218
2. In Utero Imaging of Oocyte Meiotic Divisions..........221
3. Ex Utero Imaging of Oocyte Meiotic Divisions and Early
Embryo Mitosis........................................227
4. Conclusions...........................................233
Acknowledgments..........................................234
References...............................................234
CHAPTER 12 Combining microscopy and biochemistry to study
meiotic spindle assembly in Drosophila oocytes........237
Pierre Rome, Hiroyuki Ohkura
1. Introduction..........................................238
2. Methods...............................................239
References...............................................247
CHAPTER 13 Methods to study meiosis in insect spermatocytes..249
James R. LaFountain Jr., Christopher S. Cohan
1. Introduction..........................................250
2. Methods for the Study of Meiosis in Populations
of Spermatocytes......................................252
3. Methods for Preparing Spermatocytes for Live-Cell Imaging ..254
4. Method for Ionophoretic Microinjection of Charged
Molecules Into the Cytoplasm of Living Cells..........259
5. Method for Pressure Microinjection of Cell-Permeable
Probes Into Interstitial Space Surrounding Spermatocytes
In Vitro..............................................264
References...............................................267
Contents ix
CHAPTER 14 Live-cell microscopy of meiosis in spermatocytes . 269
Hiroki Shibuya, Yoshinori Watanabe
1. Introduction...........................................270
2. Materials..............................................270
3. Methods................................................272
4. Notes.................................................274
Acknowledgments..........................................276
References...............................................276
CHAPTER 15 Live imaging of cell division in preimplantation
mouse embryos using inverted light-sheet
microscopy.............................................279
Judith Reichmann, Manuel Eguren, Yu Lin, Isabell Schneider,
Jan Ellenberg
1. Introduction..........................................280
2. Preimaging Preparations................................283
3. Live-Cell Visualization................................286
4. Materials,.............................................291
5. Conclusion.............................................291
Acknowledgments...........................................292
Conflict of Interest.................................... 292
References................................................292
CHAPTER 16 Correlated light and electron microscopy of cell
division in large marine oocytes, eggs,
and embryos........................................... 293
Mariia Burdyniuk, Natalia Wesolowska, Michal Fleszar,
Matthia A. Karreman, Pedro Machado, Joana Borrego-Pinto,
Bernhard Ruthensteiner, Yannick Schwab, Péter Lénârt
1. Introduction...........................................294
2. Protocol 1.............................................296
3. Protocol 2.............................................302
4. List of Equipment......................................310
5. List of Reagents.......................................311
Acknowledgments...........................................311
References.............................................. 312
CHAPTER 17 A microscopy-based approach for studying meiosis
in live and fixed human oocytes........................315
Agata P. Zielinska, Melina Schuh
1. Introduction...........................................316
2. Oocyte Collection and Culture..........................317
Contents
3. Studying Meiosis in Live Human Oocytes by Confocal
Microscopy..............................................321
4. Super-Resolution Microscopy of Immunolabeled Samples..327
Acknowledgments............................................333
References.................................................333
CHAPTER 18 Superresolution imaging of the synaptonemal
complex.................................................335
Katharina Schiicker, Markus Sauer, Ricardo Benavente
1. Introduction...........................................336
2. Materials............................................ 338
3. Methods................................................340
4. Conclusions............................................345
Acknowledgments............................................345
References.................................................345
CHAPTER 19 Cryo-electron tomography of SYCP3 fibers under
native conditions.......................................347
Daniel Bollschweiler, Laura Radu, Luca Pellegrini
1. Introduction...........................................348
2. Methods............................................... 350
3. Discussion.............................................369
References.................................................370
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| spelling | Mitosis and meiosis Part B edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) First edition Cambridge, MA ; San Diego, CA ; Oxford ; London Academic Press 2018 xvii, 371 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 145 Methods in cell biology Mitose (DE-588)4170185-9 gnd rswk-swf Meiose (DE-588)4169347-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mitose (DE-588)4170185-9 s Meiose (DE-588)4169347-4 s DE-604 Maiato, Helder (DE-588)116207681X edt Schuh, Melina (DE-588)136935184 edt (DE-604)BV045070188 B Methods in cell biology Volume 145 (DE-604)BV002534878 145 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030461477&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mitosis and meiosis Methods in cell biology Mitose (DE-588)4170185-9 gnd Meiose (DE-588)4169347-4 gnd |
| subject_GND | (DE-588)4170185-9 (DE-588)4169347-4 (DE-588)4143413-4 |
| title | Mitosis and meiosis |
| title_auth | Mitosis and meiosis |
| title_exact_search | Mitosis and meiosis |
| title_full | Mitosis and meiosis Part B edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) |
| title_fullStr | Mitosis and meiosis Part B edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) |
| title_full_unstemmed | Mitosis and meiosis Part B edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) |
| title_short | Mitosis and meiosis |
| title_sort | mitosis and meiosis |
| topic | Mitose (DE-588)4170185-9 gnd Meiose (DE-588)4169347-4 gnd |
| topic_facet | Mitose Meiose Aufsatzsammlung |
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