Mitosis and meiosis: Part A
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA ; San Diego, CA ; Oxford ; London
Academic Press
2018
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in cell biology
Volume 144 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xix, 474 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128141441 |
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| 245 | 1 | 0 | |a Mitosis and meiosis |n Part A |c edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) |
| 250 | |a First edition | ||
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Datensatz im Suchindex
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| adam_text | Contents
Contributors...........................................................xiii
Preface................................................................xix
CHAPTER 1 Assays for the spindle assembly checkpoint
in cell culture...........................................1
Chiara Marcozzi, Jonathon Pines
1. Introduction..............................................2
2. Choice of Fluorescent Protein Tag.........................4
3. Choice of Microscope.....................................4
4. Assays...................................................5
5. Materials................................................9
Acknowledgments........................................... 10
References..................................................10
CHAPTER 2 Quantitative methods to measure aneuploidy
and chromosomal instability..............................is
Kristina M. Godek, Duane A. Compton
1. Introduction.............................................16
2. Immunofluorescence Assay for Lagging Chromosomes
in Anaphase..............................................19
3. Chromosome Missegregation Assay..........................21
4. Aneuploid Cell Survival Assay...........................25
5. Concluding Remarks......................................30
Acknowledgments.............................................30
References..................................................30
CHAPTER 3 Dissecting the role of the tubulin code in mitosis...........33
Luisa T. Ferreira, Ana C. Figueiredo, Bernardo Orr,
Danilo Lopes, Helder Maiato
1. Introduction.............................................36
2. Modulation of the Detyrosination/Tyrosination Cycle
in Mammalian Cells.......................................40
3. Analysis of Microtubule Dynamics in Mitosis.............57
4. Identification of MAPs and Motors Binding to
(De)Tyrosinated Microtubules.............................62
5. Conclusions and Outlook......................... 66
Acknowledgments.............................................67
References................................................ 67
V
Contents
CHAPTER 4 Employing CRISPR/Ca$9 genome engineering to
dissect the molecular requirements for mitosis..........is
Kara L. McKinley
1. Introduction............................................76
2. CRISPR/Cas9 Gene Disruption in Human Cells for Acute
and Chronic Protein Elimination.........................80
3. Insertion of Tags Into Endogenous Loci to Report on
and Perturb Gene Function...............................96
4. Conclusions and Outlook................................101
Acknowledgments........................................... 101
References................................................102
CHAPTER 5 Applying the auxin-inducible degradation system
for rapid protein depletion in mammalian cells.........107
Bramwell G. Lambrus, Tyler C. Moyer, Andrew J. Holland
1- Introduction...........................................108
2. Materials Required.....................................115
3. Methods................................................116
4. Functional Analysis....................................129
5. Useful Tips............................................130
6. Troubleshooting .......................................131
7. Conclusion.............................................132
References................................................132
CHAPTER 6 Small molecule tools in mitosis research....................137
Franziska Teusel, Lars Henschke, Thomas U. Mayer
1. Introduction...........................................138
2. Inhibitors of Mitotic Kinases..........................139
3. Inhibitors of Mitotic Phosphatases.....................142
4. Inhibitors of the Cytoskeleton.........................142
5. Motor Protein Inhibitors...............................144
6. Inhibitors of the Ubiquitin Proteasome Pathway.........145
7. Protocols..............................................146
Acknowledgment............................................151
References................................................151
CHAPTER 7 Optogenetic control of mitosis with photocaged
chemical dimerizers....................................157
Huaiying Zhang, David M. Chenoweth, Michael A. Lampson
1. Introduction...........................................158
2. Materials..............................................159
3. Methods................................................161
Contents
VII
4. Notes..................................................162
5. Outlook................................................163
References................................................164
CHAPTER 8 Measuring mitotic forces....................................165
Anna A. Ye, Thomas J. Maresca
1. Introduction...........................................166
2. Selection and Placement of Sensors.....................166
3. Equipment and Materials................................168
4. FRET-BasedTS...........................................171
5. Talin-Vinculin-Based Force Sensor......................177
6. Conclusions............................................180
References................................................182
CHAPTER 9 Employing the one-cell C. elegans embryo to
study cell division processes...........................185
Neil Hattersley, Pablo Lara-Gonzalez, Dhanya Cheerambathur,
J. Sebastian Gomez-Cavazos, Taekyung Kim, Bram Prevo,
Renat Khaliullin, Kian-Yong Lee, Midori Ohta, Rebecca Green,
Karen Oegema, Arshad Desai
1. Introduction...........................................186
2. Engineering Cell Division Genes........................188
3. Monitoring of Cell Division Processes..................210
4. Conclusion/Perspective.................................223
Acknowledgments...........................................224
Appendix..................................................224
References................................................225
CHAPTER 10 A cell-free system of Drosophila egg explants
supporting native mitotic cycles........................233
Jorge de-Carvalho, Ojas Deshpande, Catarina Nabais,
Ivo A. Telley
1. Introduction...........................................234
2. Materials..............................................236
3. Microscope Setup.......................................242
4. Extract Preparation....................................246
5. Strategies for Obtaining Particular Configurations
in Explants.............................................251
6. Chemical Perturbation Experiments......................252
7. Conclusions............................................255
Acknowledgments......................................... 255
References................................................255
Contents
CHAPTER 11 Living Xenopus oocytes, eggs, and embryos
as models for cell division..........................259
Ani Varjabedian, Angela Kita, William Bement
1. Introduction.........................................260
2. Methods..............................................268
3. Construct Preparation................................271
4. Activated Egg Experiments............................273
5. Embryo Experiments...................................276
6. Mounting Samples for Live Imaging....................279
References.............................................281
CHAPTER 12 Analysis of chromosomes from mouse oocytes
and mammalian cultured cells by light
microscopy...........................................287
Mariana C.C. Silva, Gordana Wutz, Kikue Tachibana,
Jan-Michael Peters
1. Introduction.........................................288
2. Diakinesis (Prometaphase I) and Metaphase II Mouse
Oocyte Chromosome Spreads............................289
3. In Situ Fluorescence Microscopy of Metaphase II Eggs
to Assess Ploidy.....................................296
4. Spreading and Giemsa Staining of Chromosomes From
Mammalian Cultured Cells.............................298
5. Immunofluorescence Staining of Spread Chromosomes
From Mammalian Cultured Cells........................300
Acknowledgments.........................................303
References............................................ 304
CHAPTER 13 In vitro reconstitution of lateral to end-on
conversion of kinetochore-microtubule
attachments..........................................307
Manas Chakraborty, Ekaterina V. Tarasovetc,
Ekaterina L. Grishchuk
1. Introduction.........................................308
2. Materials............................................311
3. Methods..............................................318
4. Outlook..............................................324
Acknowledgments.........................................325
References..............................................325
Contents
IX
CHAPTER 14 Isolation of mitotic chromosomes from vertebrate
cells and characterization of their proteome by
mass spectrometry........................................329
Itaru Samejima, William C. Earnshaw
1. Introduction.............................................330
2. Isolation of Mitotic Chromosomes From DT40 Cells........332
3. Sample Preparation for Mass Spectrometry................336
4. Fractionation of a Complex Sample by Off-Line SCX
Column Chromatography....................................339
5. Instrument Settings of Mass Spectrometers...............341
6. The Raw File............................................342
7. Computer Programs for Analyzing Mass Spectrometry Data ...342
8. Data Analysis and Data Presentation.....................344
9. Conclusions.............................................345
Acknowledgments.............................................346
References..................................................346
CHAPTER 15 Purification of kinetochores from the budding
yeast Saccharomyces cerevisiae...........................349
Amitabha Gupta, Rena K. Evans, Lori B. Koch,
Aimee J. Littleton, Sue Biggins
1. Introduction.......................................... 350
2. Overview of Yeast Kinetochores..........................351
3. Isolation of Yeast Kinetochores.........................354
4. Differences in Kinetochore Composition Based
on Purification..........................................362
5. Conclusions.............................................366
Acknowledgments........................................... 367
References..................................................368
CHAPTER 16 Characterization of DNA helicases and nucleases
from meiotic extracts of S. cerevisiae...................371
Rokas Grigaitis, Aitor Susperregui, Philipp Wild,
ioao Matos
1. Introduction........................................... 372
2. Synchronization and Harvesting of Large Meiotic Cultures
of 5. cerevisiae..............................*.........373
3. Purification and Characterization of the STR Complex
From Prophase 1..........................................376
4. Functional Analysis of Sgsl Helicase and Mus81-Mms4
Nuclease From Meiotic Extracts...........................379
Contents
5. Adaptation to Other DNA Processing Enzymes and DNA
Substrates............................................. 387
Acknowledgments............................................387
Author Contributions.......................................387
References.................................................388
CHAPTER 17 Single-nucleus Hi-C of mammalian oocytes
and zygotes..............................................389
Johanna Gassier, Ilya M. Flyanter, Kikue Tachibana
1. Introduction............................................390
2. Materials...............................................392
3. Single-Nucleus Hi-C.....................................395
4. Notes...................................................401
5. Discussion..............................................403
Acknowledgments............................................404
References.................................................405
CHAPTER 18 Induction of fetal primary oocytes and
the meiotic prophase from mouse piuripotent
stem cells...............................................409
Hidetaka Miyauchi, Hiroshi 0htaf Mitinori Saitou
1. Introduction............................................410
2. Preparation of Feeder Cells.............................413
3. The Induction of PGCLCs From Piuripotent Stem Cells.....416
4. Propagation of PGCLCs, and Induction of Fetal
Primary Oocytes and Meiosis From PGCLCs..................419
5. Materials and Reagents..................................422
6. Conclusion..............................................424
Acknowledgments............................................426
References.................................................426
CHAPTER 19 Electrical-assisted microinjection for analysis
of fertilization and cell division in mammalian
oocytes and early embryos................................431
Greg FitzHarris, John Carroll, Karl Swann
1. Introduction............................................432
2. Notes and Technical Suggestions.........................437
3. Materials/Key Equipment.................................438
References.................................................439
Contents xi
CHAPTER 20 Single cell genomics to study DNA and
chromosome changes in human gametes
and embryos.......................................... 441
Robert C. Blanshard, Chongyi Chen, Xiaoliang Sunney Xie,
Eva R. Hoffmann
1. Introduction...........................................442
2. Methods................................................443
3. Conclusions............................................455
Acknowledgments...........................................456
References................................................456
CHAPTER 21 Cytoplasmic removal, enucleation, and
cell fusion of mouse oocytes........................... 459
Hirohisa Kyogoku, Shuhei Yoshida, Tomoya S. Kitajima
1. Introduction...........................................460
2. Isolation of GV-stage Oocytes..........................461
3. Cytoplasmic Removal....................................462
4. Enucleation and Oocyte Fusion........................ 464
5. Spindle Fusion.........................................467
6. Materials..............................................470
7. Discussion.............................................471
Acknowledgments...........................................472
References............................................ 473
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| spelling | Mitosis and meiosis Part A edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) First edition Cambridge, MA ; San Diego, CA ; Oxford ; London Academic Press 2018 xix, 474 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 144 Methods in cell biology Meiose (DE-588)4169347-4 gnd rswk-swf Mitose (DE-588)4170185-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mitose (DE-588)4170185-9 s Meiose (DE-588)4169347-4 s DE-604 Maiato, Helder (DE-588)116207681X edt Schuh, Melina (DE-588)136935184 edt (DE-604)BV045070188 A Methods in cell biology Volume 144 (DE-604)BV002534878 144 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030461469&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Mitosis and meiosis |
| title_auth | Mitosis and meiosis |
| title_exact_search | Mitosis and meiosis |
| title_full | Mitosis and meiosis Part A edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) |
| title_fullStr | Mitosis and meiosis Part A edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) |
| title_full_unstemmed | Mitosis and meiosis Part A edited by Helder Maiato (Instituto de Biologia Molecular e Celular; i3S - Instituto de Investigação e Inovação em Saúde; Faculdade de Medicina, Universidade do Porto, Porto, Portugal), Melina Schuh (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) |
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