Speeches for the dead: essays on Plato's Menexenus
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin ; Boston
De Gruyter
[2018]
|
| Schriftenreihe: | Beiträge zur Altertumskunde
Band 368 |
| Schlagworte: | |
| Online-Zugang: | http://www.degruyter.com/search?f_0=isbnissn&q_0=9783110570731&searchTitles=true Inhaltsverzeichnis |
| Beschreibung: | 202 Seiten 23 cm x 15.5 cm |
| ISBN: | 9783110570731 |
Internformat
MARC
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| 245 | 1 | 0 | |a Speeches for the dead |b essays on Plato's Menexenus |c edited by Harold Parker and Jan Maximilian Robitzsch |
| 264 | 1 | |a Berlin ; Boston |b De Gruyter |c [2018] | |
| 264 | 4 | |c © 2018 | |
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| 653 | |a Menexenus | ||
| 653 | |a Plato | ||
| 653 | |a funeral oration | ||
| 653 | |a historiography | ||
| 653 | |a Geschichtsschreibung | ||
| 653 | |a Gefallenenrede | ||
| 653 | |a Menexenos | ||
| 653 | |a Platon | ||
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 655 | 7 | |0 (DE-588)1071861417 |a Konferenzschrift |y 2014 |z Philadelphia |2 gnd-content | |
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Datensatz im Suchindex
| _version_ | 1804178620390834176 |
|---|---|
| adam_text | TABLE OF CONTENTS
SUMMARY I
ZUSAMMENFASSUNG II
TABLE OF CONTENTS IV
ABBREVIATIONS VII
1. INTRODUCTION 1
1.1 PROTEIN IMPORT INTO CHLOROPLASTS 1
1.2 TIC22 AND ITS EVOLUTIONARY CONSERVATION 4
1.4 THE FUNCTION OF TIC62 AND TROL AND THEIR INTERPLAY WITH FNR 6
1.5 METABOLIC PROCESSES CONNECTED TO PHOTOSYNTHESIS 7
1.6 AIMS OF THIS WORK 9
2. MATERIALS 10
2.1 CHEMICALS 10
2.2 ENZYMES 10
2.3 OLIGONUCLEOTIDES 10
2.4 VECTORS AND CONSTRUCTS 12
2.5 MOLECULAR WEIGHT MARKERS AND DNA STANDARDS 13
2.6 ANTIBODIES 13
2.7 STRAINS 13
2.8 PLANT MATERIAL 13
3. METHODS 14
3.1 PLANT METHODS 14
3.1.1 GROWTH OF A THALIANA 14
3.1.2 CROSS FERTILIZATION OF A THALIANA 14
3.1.3 STABLE TRANSFORMATION OF A THALIANA 15
3.1.4 CHLOROPHYLL FLUORESCENCE MEASUREMENTS OF PSII 15
3.2 MICROBIOLOGY METHODS 15
3.2.1 MEDIA AND GROWTH 15
3.2.2 BACTERIA TRANSFORMATION 16
3.3 MOLECULAR BIOLOGY METHODS 16
3.3.1 POLYMERASE CHAIN REACTION (PCR) 16
3.3.2 CLONING STRATEGIES 17
3.3.3 ISOLATION OF DNA PLASMIDS FROM ESCHERICHIA COLI 17
IV
HTTP://D-NB.INFO/1047864185
TABLE OF CONTENTS
3.3.4 PREPARATION OF GENOMIC DNAOF A THALIANA 17
3.3.5 DETERMINATION OF DNA AND RNA CONCENTRATIONS 17
3.3.6 CHARACTERIZATION OF PLANT T-DNA INSERTION LINES 18
3.3.7 DNA SEQUENCING 18
3.3.8 RNA EXTRACTION AND REAL-TIME RT-PCR (QRT-PCR) 18
3.3.9 MICROARRAY ANALYSIS 19
3.4 BIOCHEMICAL METHODS 19
3.4.1 DETERMINATION OF PROTEIN CONCENTRATION 19
3.4.2 PROTEIN EXTRACTION OF A THALIANA 19
3.4.3 SDS-PAGE (POLYACRYLAMIDE GELELECTROPHORESIS) 20
3.4.4 IMMUNODETECTION 20
3.4.5 BLUE NATIVE PAGE 21
3.4.6 IN VITRO TRANSCRIPTION AND TRANSLATION 21
3.4.7 A THALIANA CHLOROPLAST ISOLATION 22
3.4.8 A THALIANA CHLOROPLAST ISOLATION AND FRACTIONATION 22
3.4.9 PISUM SATIVUM CHLOROPLAST ISOLATION AND PROTEIN IMPORT 22
3.4.10 PROTEIN EXPRESSION, PURIFICATION AND CRYSTALLIZATION 23
3.5 METABOLITE ANALYSIS 23
3.6 MICROSCOPY 24
3.7 COMPUTATIONAL ANALYSIS 24
4. RESULTS 25
4.1 CHARACTERIZATION OF TIC22 IN A THALIANA 25
4.1.1 THE TIC22 PROTEIN FAMILY IN A THALIANA 25
4.1.2 GENERATION OF TIC22 MUTANTS IN A THALIANA 29
4.1.3 PHENOTYPIC CHARACTERIZATION OF THE TIC22LLLXLV DOUBLE MUTANT 31
4.1.4 PHOTOSYNTHETIC PERFORMANCE OF WT AND TIC22DM PLANTS 36
4.1.5 MOLECULAR ANALYSIS OF SEPARATED LEAF PARTS 37
4.1.6 IMMUNOBLOT OF LHC PROTEINS IN A THALIANA WT AND TIC22DM
CHLOROPLASTS 41
4.1.7 ANALYSIS OF THE GENE REGULATION IN WT AND TIC22DM BY
DNA-MICROARRAY ANALYSIS 42
4.1.8 GENE SILENCING OF TIC22V BY RNAI 46
4.1.9 ISOLATION OF TIC22V KNOCK-OUT MUTANTS 49
4.1.10 LOCALIZATION OF TIC22V 51
4.1.11 STRUCTURAL ANALYSIS OF SYNECHOCYSTIS SP. TIC22 BY X-RAY
CRYSTALLOGRAPHY 52
4.2 CHARACTERIZATION OF TIC62/TROL AND THEIR RELATION TO FNR 57
4.2.1 GENERATING TIC62TROL DOUBLE MUTANTS 57
V
TABLE OF CONTENTS
4.2.2 LOCALIZATION OF FNR IN TIC62TROL 60
4.2.3 THYLAKOIDAL COMPLEX COMPOSITION IN TIC62TROL 61
4.2.4 PHENOTYPIC AND PHOTOSYNTHETIC ANALYSIS OF TIC62TROL 63
4.2.5 METABOLIC PATHWAYS INFLUENCED IN TIC62TROL PLANTS 63
5. DISCUSSION 67
5.1 CHARACTERIZATION OF TIC22 IN A.THALIANA 67
5.1.1 GENERAL PROPERTIES OF THE TIC22 ISOFORMS IN ATHALIANA 67
5.1.2 TIC22V MIGHT PLAY A ROLE DURING POLLEN DEVELOPMENT 69
5.1.3 THE KNOCK-OUT OF TIC22LLL AND TIC22IV LEADS TO A CHLOROTIC
PHENOTYPE AND ALTERED THYLAKOID
COMPOSITION 69
5.1.4 TIC22DM REVEALS AN ALTERED GENE EXPRESSION 70
5.1.5 TIC22DM REVEALS A REDUCED OE33 CONTENT IN THE MARGINS 71
5.1.6 TIC22DM PLANTS CONTAIN AN ALTERED PLASTID GENE EXPRESSION 71
5.1.7 POTENTIAL FLEXIBLE LOOPS MIGHT AVOID THE CRYSTALLIZATION OF
SYNTIC22 73
5.2 TIC62 AND TROL AND THEIR POTENTIAL ROLE IN PHOTOSYNTHETIC PROCESSES
76
6. REFERENCES 80
7. SUPPLEMENTARY DATA 91
7.1 DNA MICROARRAY ANALYSIS 91
CURRICULUM VITAE 100
DANKSAGUNG 101
EIDESSTATTLICHE VERSICHERUNG 102
ERKLARUNG 102
VI
|
| any_adam_object | 1 |
| author2 | Parker, Harold Robitzsch, Jan Maximilian |
| author2_role | edt edt |
| author2_variant | h p hp j m r jm jmr |
| author_GND | (DE-588)1147206295 |
| author_facet | Parker, Harold Robitzsch, Jan Maximilian |
| building | Verbundindex |
| bvnumber | BV045009173 |
| classification_rvk | FH 28715 |
| ctrlnum | (OCoLC)1047864185 (DE-599)DNB1139378821 |
| dewey-full | 930 |
| dewey-hundreds | 900 - History & geography |
| dewey-ones | 930 - History of ancient world to ca. 499 |
| dewey-raw | 930 |
| dewey-search | 930 |
| dewey-sort | 3930 |
| dewey-tens | 930 - History of ancient world to ca. 499 |
| discipline | Geschichte Philologie / Byzantinistik / Neulatein |
| format | Book |
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| spelling | Speeches for the dead essays on Plato's Menexenus edited by Harold Parker and Jan Maximilian Robitzsch Berlin ; Boston De Gruyter [2018] © 2018 202 Seiten 23 cm x 15.5 cm txt rdacontent n rdamedia nc rdacarrier Beiträge zur Altertumskunde Band 368 Plato v427-v347 Menexenus (DE-588)4365103-3 gnd rswk-swf Menexenus Plato funeral oration historiography Geschichtsschreibung Gefallenenrede Menexenos Platon (DE-588)4143413-4 Aufsatzsammlung gnd-content (DE-588)1071861417 Konferenzschrift 2014 Philadelphia gnd-content Plat. Menexenus (DE-2581)TH000002402 gbd Plato phil. TLG 0059 (DE-2581)TH000002380 gbd Plato v427-v347 Menexenus (DE-588)4365103-3 u DE-604 Parker, Harold edt Robitzsch, Jan Maximilian (DE-588)1147206295 edt Walter de Gruyter GmbH & Co. KG (DE-588)10095502-2 pbl Erscheint auch als Online-Ausgabe, ePDF 978-3-11-057589-7 Erscheint auch als Online-Ausgabe, ePub 978-3-11-057397-8 Beiträge zur Altertumskunde Band 368 (DE-604)BV004237215 368 X:MVB http://www.degruyter.com/search?f_0=isbnissn&q_0=9783110570731&searchTitles=true DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030401145&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Speeches for the dead essays on Plato's Menexenus Beiträge zur Altertumskunde Plato v427-v347 Menexenus (DE-588)4365103-3 gnd |
| subject_GND | (DE-588)4365103-3 (DE-588)4143413-4 (DE-588)1071861417 |
| title | Speeches for the dead essays on Plato's Menexenus |
| title_auth | Speeches for the dead essays on Plato's Menexenus |
| title_exact_search | Speeches for the dead essays on Plato's Menexenus |
| title_full | Speeches for the dead essays on Plato's Menexenus edited by Harold Parker and Jan Maximilian Robitzsch |
| title_fullStr | Speeches for the dead essays on Plato's Menexenus edited by Harold Parker and Jan Maximilian Robitzsch |
| title_full_unstemmed | Speeches for the dead essays on Plato's Menexenus edited by Harold Parker and Jan Maximilian Robitzsch |
| title_short | Speeches for the dead |
| title_sort | speeches for the dead essays on plato s menexenus |
| title_sub | essays on Plato's Menexenus |
| topic | Plato v427-v347 Menexenus (DE-588)4365103-3 gnd |
| topic_facet | Plato v427-v347 Menexenus Aufsatzsammlung Konferenzschrift 2014 Philadelphia |
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