Oameni şi întâmplări din Bucovina: Vol. 2
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | Romanian |
| Veröffentlicht: |
Câmpulung, Bucovina
Ed. Fundatia Culturală Alexandru Bogza
2003
Iaşi Editura "Bucovina" 2003 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 241 Seiten |
| ISBN: | 9739988733 |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
VORVEROEFFENTLICHUNGEN DER DISSERTATION 3
ABSTRACT 12
ZUSAMMENFASSUNG 14
1 INTRODUCTION 16
1.1 BALANCING IMMUNE
RESPONSES 16
1.2 IMMUNOLOGICAL TOLERANCE 18
1.3 REGULATORY T CELIS 20
1.4 DIFFERENTIATION OF FOXP3* TREGS 21
1.4.1 INTRATHYMIC DIFFERENTIATION 21
1.4.2 EXTRATHYMIC DIFFERENTIATION 22
1.5 PHENOTYPIC FEATURES AND FUNCTIONAL MECHANISM 24
1.6 MUCOSAL TOLERANCE 25
1.6.1 REGULATORY RESPONSES AT GASTRIC
MUCOSAL SITES 25
1.6.2 ORAL TOLERANCE 26
1.7 FUNCTIONS OF TREGS
DURING INFECTION 27
1.8 ENTERIC BACTERIAL PATHOGENS 29
1.8.1 THE GENUS YERSINIA 29
1.8.2 MODE OF INFECTION 30
1.8.3 BREACH OF MUCOSAL
BARRIER 31
1.8.4 IMMUNE RESPONSE TO YERSINIA
INFECTION 32
1.8.5 CELLULAR IMMUNE RESPONSE TO YERSINIA
INFECTION 34
2 AIM OF THE STUDY 37
3 MATERIAL AND METHODS 39
3.1 INSTRUMENTATION 39
3.2 CHEMICALS AND REAGENTS 39
3.3 BUFFERS AND MEDIA 41
3.4 MICE 41
3.4.1 MOUSE INFECTION 42
3.5 BACTERIAL STRAINS, MEDIA AND
GROWTH CONDITIONS 43
3.5.1 CONSTRUCTION OF STRAIN 43
3.6 LYMPHOCYTE PREPARATION 44
3.7 ENUMERATION OF LYMPHOCYTES 44
3.8 FLOW CYTOMETRY 45
3.8.1 STAINING OF GRANULOCYTES FROM PERIPHERAL
BLOOD 45
9
HTTP://D-NB.INFO/1038756456
3.8.2 ANTIBODY STAINING 45
3.9 ADOPTIVE TRANSFER OF NAIVE T CELLS 48
3.9.1 NAIVE T CELL ISOLATION FROM
D011.10 MICE 49
3.9.2 NAIVE T CELL
ISOLATION FROM D011.10XFOXP3
9FP
MICE 49
3.10 GENERATION OF MIXED
BONE MARROW CHIMERIC
MICE 50
3.11 IN VIVO TREG INDUCTION 51
3.11.1 DUERING YPTB INFECTION 51
3.11.2 UPON PARTIAL DEPLETION
OF CD11C* DCS 51
3.12 SCANNING ELECTRON MICROSCOPY 51
3.13 DETECTION OF SS-LACTAMASE ACTIVITY 52
3.13.1 MONITORING SS-LACTAMASE ACTIVITY
UPON IN VITRO
CO-CULTURE 52
3.13.2 ANALYSIS OF SS-LACTAMASE
ACTIVITY UPON
MURINE INFECTION 53
3.14 SUPPRESSION ASSAYS 54
3.15 TREG DEPLETION
IN DEREG MICE 55
3.16 STATISTICS 55
4 RESULTS 56
4.1 DEVELOPMENT OF A SUBLETHAL
INTRAGASTRIC INFECTION MODEL
THAT ALLOWED
CHARACTERIZATION OF IMMUNE RESPONSES TO YPTB IN MICE 56
4.2 YPTB INDUCES LOSS OF CONVENTIONAL DCS 60
4.3 YPTB INFECTION REDUCES
TREG-INDUCING POTENTIAL
OF GUT-DRAINING LNS 61
4.4 YPTB INDUCES ALTERATIONS
OF SURFACE MOLECULE EXPRESSION
IN CDCS 65
4.5 TRANSIENT DEPLETION OF CD11
C*DC IMPAIRS EFFICIENT
IN VIVO TREG
INDUCTION
.67
4.6 TRACKING YPTB YOP INJECTION
INTO EUKARYOTIC CELLS 71
4.7 ENHANCED YOP TRANSLOCATION
INTO CD4*CD25*
TREGS UPON EX VIVO INFECTION..
74
4.8 MONITORING YOP DELIVERY
IN PP, MLN
AND SPIEEN UPON
ORAL INFECTION 75
4.9 EFFECT OF YPTB ON SUPPRESSIVE ACTIVITY OF TREGS 78
4.10 FUNCTIONAL IMPORTANCE OF FOXP3* TREGS DURING
YPTB INFECTION 83
4.10.1 IMPACT OF YPTB
(IP32953) INFECTION ON IMMUNE CELL COMPOSITION 83
4.10.2 YPTB (IP32953)
MODULATES THE INNATE
IMMUNE RESPONSE 85
4.10.3 ALTERATIONS IN THE
COMPOSITION OF THE T CELL COMPARTMENT UPON
YPTB
(IP32953) INFECTION 88
4.10.1 DT MEDIATED TREGS DEPLETION
DOES NOT PROMOTE BACTERIAL
CLEARANCE...
89
5 DISCUSSION 95
5.1 INFECTION-MEDIATED LOSS OF PERIPHERAL TREG INDUCTION 95
5.2 PARTIAL LOSS
OF DCS HINDERE
PERIPHERAL ITREG
GENERATION 100
5.3 MODULATION OF TREGS BY YPTB 101
10
5.3.1 TREGS ARE PREFERENTIAL TARGETED BY YPTB 101
5.3.2 YPTB MEDIATED LOSS
IN IMMUNOSUPPRESSIVE MECHANISM 105
5.4 FUNCTIONAL IMPORTANCE OF TREGS 107
5.4.1 YPTB INFECTION RESULTS
IN LOSS
OF TREGS IN VIVO 107
5.4.1 ABLATION OF TREGS DOES
NOT ALTER BACTERIAL
COLONIZATION 109
6 SUMMARY 112
7 REFERENCES 114
8 ABBREVIATIONS 131
9 ACKNOWLEDGEMENTS 134
11
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| title_full | Oameni şi întâmplări din Bucovina Vol. 2 Dragoş Nisioiu |
| title_fullStr | Oameni şi întâmplări din Bucovina Vol. 2 Dragoş Nisioiu |
| title_full_unstemmed | Oameni şi întâmplări din Bucovina Vol. 2 Dragoş Nisioiu |
| title_short | Oameni şi întâmplări din Bucovina |
| title_sort | oameni si intamplari din bucovina |
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