High Throughput Protein Expression and Purification: Methods and Protocols
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in us...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2009
|
| Schriftenreihe: | Methods in Molecular Biology
498 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields |
| Beschreibung: | 1 Online-Ressource (XII, 322 p. 57 illus., 6 illus. in color) |
| ISBN: | 9781597451963 |
| DOI: | 10.1007/978-1-59745-196-3 |
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Datensatz im Suchindex
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| spelling | High Throughput Protein Expression and Purification Methods and Protocols edited by Sharon A. Doyle Totowa, NJ Humana Press 2009 1 Online-Ressource (XII, 322 p. 57 illus., 6 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 498 Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields Life Sciences Biochemistry, general Protein Science Protein Structure Life sciences Biochemistry Proteins Proteine (DE-588)4076388-2 gnd rswk-swf Trennverfahren (DE-588)4078395-9 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Reinigungsverfahren (DE-588)4391913-3 gnd rswk-swf Proteine (DE-588)4076388-2 s Genexpression (DE-588)4020136-3 s DE-604 Trennverfahren (DE-588)4078395-9 s Reinigungsverfahren (DE-588)4391913-3 s Doyle, Sharon A. edt Erscheint auch als Druck-Ausgabe 9781588298799 https://doi.org/10.1007/978-1-59745-196-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | High Throughput Protein Expression and Purification Methods and Protocols Life Sciences Biochemistry, general Protein Science Protein Structure Life sciences Biochemistry Proteins Proteine (DE-588)4076388-2 gnd Trennverfahren (DE-588)4078395-9 gnd Genexpression (DE-588)4020136-3 gnd Reinigungsverfahren (DE-588)4391913-3 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4078395-9 (DE-588)4020136-3 (DE-588)4391913-3 |
| title | High Throughput Protein Expression and Purification Methods and Protocols |
| title_auth | High Throughput Protein Expression and Purification Methods and Protocols |
| title_exact_search | High Throughput Protein Expression and Purification Methods and Protocols |
| title_full | High Throughput Protein Expression and Purification Methods and Protocols edited by Sharon A. Doyle |
| title_fullStr | High Throughput Protein Expression and Purification Methods and Protocols edited by Sharon A. Doyle |
| title_full_unstemmed | High Throughput Protein Expression and Purification Methods and Protocols edited by Sharon A. Doyle |
| title_short | High Throughput Protein Expression and Purification |
| title_sort | high throughput protein expression and purification methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Protein Science Protein Structure Life sciences Biochemistry Proteins Proteine (DE-588)4076388-2 gnd Trennverfahren (DE-588)4078395-9 gnd Genexpression (DE-588)4020136-3 gnd Reinigungsverfahren (DE-588)4391913-3 gnd |
| topic_facet | Life Sciences Biochemistry, general Protein Science Protein Structure Life sciences Biochemistry Proteins Proteine Trennverfahren Genexpression Reinigungsverfahren |
| url | https://doi.org/10.1007/978-1-59745-196-3 |
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