DNA-Protein Interactions: Principles and Protocols
DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new sta...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology
148 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions |
| Beschreibung: | 1 Online-Ressource (656 p. 146 illus) |
| ISBN: | 9781592592081 |
| DOI: | 10.1385/1592592082 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044952439 | ||
| 003 | DE-604 | ||
| 005 | 20180905 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2001 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592592081 |9 978-1-59259-208-1 | ||
| 024 | 7 | |a 10.1385/1592592082 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-208-1 | ||
| 035 | |a (OCoLC)730584144 | ||
| 035 | |a (DE-599)BVBBV044952439 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 050 | 0 | |a QP624.75.P74 | |
| 082 | 0 | |a 571.6 |2 23 | |
| 082 | 0 | |a 572.8/6 |2 21 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a WD 5360 |0 (DE-625)148202: |2 rvk | ||
| 084 | |a CHE 860f |2 stub | ||
| 084 | |a CHE 820f |2 stub | ||
| 245 | 1 | 0 | |a DNA-Protein Interactions |b Principles and Protocols |c edited by Tom Moss |
| 250 | |a Second Edition | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
| 300 | |a 1 Online-Ressource (656 p. 146 illus) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology |v 148 | |
| 520 | |a DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Cell biology | |
| 650 | 0 | 7 | |a DNS |0 (DE-588)4070512-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Wechselwirkung |0 (DE-588)4064937-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Proteine |0 (DE-588)4076388-2 |D s |
| 689 | 0 | 1 | |a DNS |0 (DE-588)4070512-2 |D s |
| 689 | 0 | 2 | |a Wechselwirkung |0 (DE-588)4064937-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Moss, Tom |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9780896036253 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/1592592082 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030345194 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1385/1592592082 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1385/1592592082 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178542315962368 |
|---|---|
| any_adam_object | |
| author2 | Moss, Tom |
| author2_role | edt |
| author2_variant | t m tm |
| author_facet | Moss, Tom |
| building | Verbundindex |
| bvnumber | BV044952439 |
| callnumber-first | Q - Science |
| callnumber-label | QP624 |
| callnumber-raw | QP624.75.P74 |
| callnumber-search | QP624.75.P74 |
| callnumber-sort | QP 3624.75 P74 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4150 WD 5360 |
| classification_tum | CHE 860f CHE 820f |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-208-1 (OCoLC)730584144 (DE-599)BVBBV044952439 |
| dewey-full | 571.6 572.8/6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects 572 - Biochemistry |
| dewey-raw | 571.6 572.8/6 |
| dewey-search | 571.6 572.8/6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| doi_str_mv | 10.1385/1592592082 |
| edition | Second Edition |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03653nmm a2200613zcb4500</leader><controlfield tag="001">BV044952439</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180905 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2001 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592592081</subfield><subfield code="9">978-1-59259-208-1</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/1592592082</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-208-1</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)730584144</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044952439</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QP624.75.P74</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.8/6</subfield><subfield code="2">21</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5360</subfield><subfield code="0">(DE-625)148202:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 860f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 820f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">DNA-Protein Interactions</subfield><subfield code="b">Principles and Protocols</subfield><subfield code="c">edited by Tom Moss</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">Second Edition</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2001</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (656 p. 146 illus)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield><subfield code="v">148</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Biology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell biology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">DNS</subfield><subfield code="0">(DE-588)4070512-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Wechselwirkung</subfield><subfield code="0">(DE-588)4064937-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">DNS</subfield><subfield code="0">(DE-588)4070512-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Wechselwirkung</subfield><subfield code="0">(DE-588)4064937-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Moss, Tom</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9780896036253</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/1592592082</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030345194</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1592592082</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1592592082</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044952439 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:37Z |
| institution | BVB |
| isbn | 9781592592081 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030345194 |
| oclc_num | 730584144 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (656 p. 146 illus) |
| psigel | ZDB-2-PRO |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology |
| spelling | DNA-Protein Interactions Principles and Protocols edited by Tom Moss Second Edition Totowa, NJ Humana Press 2001 1 Online-Ressource (656 p. 146 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 148 DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions Life Sciences Cell Biology Life sciences Cell biology DNS (DE-588)4070512-2 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s DNS (DE-588)4070512-2 s Wechselwirkung (DE-588)4064937-4 s DE-604 Moss, Tom edt Erscheint auch als Druck-Ausgabe 9780896036253 https://doi.org/10.1385/1592592082 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | DNA-Protein Interactions Principles and Protocols Life Sciences Cell Biology Life sciences Cell biology DNS (DE-588)4070512-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4070512-2 (DE-588)4064937-4 (DE-588)4076388-2 (DE-588)4143413-4 |
| title | DNA-Protein Interactions Principles and Protocols |
| title_auth | DNA-Protein Interactions Principles and Protocols |
| title_exact_search | DNA-Protein Interactions Principles and Protocols |
| title_full | DNA-Protein Interactions Principles and Protocols edited by Tom Moss |
| title_fullStr | DNA-Protein Interactions Principles and Protocols edited by Tom Moss |
| title_full_unstemmed | DNA-Protein Interactions Principles and Protocols edited by Tom Moss |
| title_short | DNA-Protein Interactions |
| title_sort | dna protein interactions principles and protocols |
| title_sub | Principles and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology DNS (DE-588)4070512-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology DNS Wechselwirkung Proteine Aufsatzsammlung |
| url | https://doi.org/10.1385/1592592082 |
| work_keys_str_mv | AT mosstom dnaproteininteractionsprinciplesandprotocols |