Confocal Microscopy: Methods and Protocols
Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging o...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2014
|
| Ausgabe: | 2nd ed. 2014 |
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1075 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques. |
| Beschreibung: | 1 Online-Ressource (XI, 381 p. 130 illus., 51 illus. in color) |
| ISBN: | 9781607618478 |
| DOI: | 10.1007/978-1-60761-847-8 |
Internformat
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| doi_str_mv | 10.1007/978-1-60761-847-8 |
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| spelling | Confocal Microscopy Methods and Protocols edited by Stephen W. Paddock 2nd ed. 2014 New York, NY Springer New York 2014 1 Online-Ressource (XI, 381 p. 130 illus., 51 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1075 Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques. Medicine & Public Health Imaging / Radiology Biological Microscopy Medicine Radiology Microscopy Konfokale Mikroskopie (DE-588)4336446-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Konfokale Mikroskopie (DE-588)4336446-9 s DE-604 Paddock, Stephen W. edt Erscheint auch als Druck-Ausgabe 9781588293510 https://doi.org/10.1007/978-1-60761-847-8 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Confocal Microscopy Methods and Protocols Medicine & Public Health Imaging / Radiology Biological Microscopy Medicine Radiology Microscopy Konfokale Mikroskopie (DE-588)4336446-9 gnd |
| subject_GND | (DE-588)4336446-9 (DE-588)4143413-4 |
| title | Confocal Microscopy Methods and Protocols |
| title_auth | Confocal Microscopy Methods and Protocols |
| title_exact_search | Confocal Microscopy Methods and Protocols |
| title_full | Confocal Microscopy Methods and Protocols edited by Stephen W. Paddock |
| title_fullStr | Confocal Microscopy Methods and Protocols edited by Stephen W. Paddock |
| title_full_unstemmed | Confocal Microscopy Methods and Protocols edited by Stephen W. Paddock |
| title_short | Confocal Microscopy |
| title_sort | confocal microscopy methods and protocols |
| title_sub | Methods and Protocols |
| topic | Medicine & Public Health Imaging / Radiology Biological Microscopy Medicine Radiology Microscopy Konfokale Mikroskopie (DE-588)4336446-9 gnd |
| topic_facet | Medicine & Public Health Imaging / Radiology Biological Microscopy Medicine Radiology Microscopy Konfokale Mikroskopie Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-60761-847-8 |
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