Plant Organogenesis: Methods and Protocols
Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as th...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2013
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
959 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating research field |
| Beschreibung: | 1 Online-Ressource (XVI, 356 p. 41 illus., 24 illus. in color) |
| ISBN: | 9781627032216 |
| DOI: | 10.1007/978-1-62703-221-6 |
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| 520 | |a Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating research field | ||
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| dewey-ones | 580 - Plants |
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| spelling | Plant Organogenesis Methods and Protocols edited by Ive De Smet Totowa, NJ Humana Press 2013 1 Online-Ressource (XVI, 356 p. 41 illus., 24 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 959 Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating research field Life Sciences Plant Sciences Plant Biochemistry Life sciences Plant biochemistry Plant science Botany Pflanzen (DE-588)4045539-7 gnd rswk-swf Organogenese (DE-588)4266352-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzen (DE-588)4045539-7 s Organogenese (DE-588)4266352-0 s b DE-604 De Smet, Ive edt Erscheint auch als Druck-Ausgabe 9781627032209 https://doi.org/10.1007/978-1-62703-221-6 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Plant Organogenesis Methods and Protocols Life Sciences Plant Sciences Plant Biochemistry Life sciences Plant biochemistry Plant science Botany Pflanzen (DE-588)4045539-7 gnd Organogenese (DE-588)4266352-0 gnd |
| subject_GND | (DE-588)4045539-7 (DE-588)4266352-0 (DE-588)4143413-4 |
| title | Plant Organogenesis Methods and Protocols |
| title_auth | Plant Organogenesis Methods and Protocols |
| title_exact_search | Plant Organogenesis Methods and Protocols |
| title_full | Plant Organogenesis Methods and Protocols edited by Ive De Smet |
| title_fullStr | Plant Organogenesis Methods and Protocols edited by Ive De Smet |
| title_full_unstemmed | Plant Organogenesis Methods and Protocols edited by Ive De Smet |
| title_short | Plant Organogenesis |
| title_sort | plant organogenesis methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Plant Sciences Plant Biochemistry Life sciences Plant biochemistry Plant science Botany Pflanzen (DE-588)4045539-7 gnd Organogenese (DE-588)4266352-0 gnd |
| topic_facet | Life Sciences Plant Sciences Plant Biochemistry Life sciences Plant biochemistry Plant science Botany Pflanzen Organogenese Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-62703-221-6 |
| work_keys_str_mv | AT desmetive plantorganogenesismethodsandprotocols |