Green Fluorescent Protein: Applications and Protocols
Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2002
|
| Schriftenreihe: | Methods in Molecular Biology
183 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems. Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques |
| Beschreibung: | 1 Online-Ressource (XIV, 394 p) |
| ISBN: | 9781592592807 |
| DOI: | 10.1385/1592592805 |
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| spelling | Green Fluorescent Protein Applications and Protocols edited by Barry W. Hicks Totowa, NJ Humana Press 2002 1 Online-Ressource (XIV, 394 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 183 Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems. Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd rswk-swf Grün fluoreszierendes Protein (DE-588)4508128-1 gnd rswk-swf Grün fluoreszierendes Protein (DE-588)4508128-1 s Labortechnik (DE-588)4123602-6 s DE-604 Hicks, Barry W. edt Erscheint auch als Druck-Ausgabe 9780896039056 https://doi.org/10.1385/1592592805 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Green Fluorescent Protein Applications and Protocols Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Grün fluoreszierendes Protein (DE-588)4508128-1 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4508128-1 |
| title | Green Fluorescent Protein Applications and Protocols |
| title_auth | Green Fluorescent Protein Applications and Protocols |
| title_exact_search | Green Fluorescent Protein Applications and Protocols |
| title_full | Green Fluorescent Protein Applications and Protocols edited by Barry W. Hicks |
| title_fullStr | Green Fluorescent Protein Applications and Protocols edited by Barry W. Hicks |
| title_full_unstemmed | Green Fluorescent Protein Applications and Protocols edited by Barry W. Hicks |
| title_short | Green Fluorescent Protein |
| title_sort | green fluorescent protein applications and protocols |
| title_sub | Applications and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Grün fluoreszierendes Protein (DE-588)4508128-1 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Labortechnik Grün fluoreszierendes Protein |
| url | https://doi.org/10.1385/1592592805 |
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