PCR Cloning Protocols:
In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-s...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2002
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology™
192 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented. Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination-essential methods for today's study of functional genomics, gene expression, protein structure-function relationships, protein engineering, and molecular evolution |
| Beschreibung: | 1 Online-Ressource (XIV, 439 p) |
| ISBN: | 9781592591770 |
| DOI: | 10.1385/1592591779 |
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| 520 | |a In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented. Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination-essential methods for today's study of functional genomics, gene expression, protein structure-function relationships, protein engineering, and molecular evolution | ||
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Datensatz im Suchindex
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| any_adam_object | |
| author2 | Chen, Bing-Yuan Janes, Harry W. |
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| discipline | Biologie |
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| spelling | PCR Cloning Protocols edited by Bing-Yuan Chen, Harry W. Janes Second Edition Totowa, NJ Humana Press 2002 1 Online-Ressource (XIV, 439 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 192 In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented. Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination-essential methods for today's study of functional genomics, gene expression, protein structure-function relationships, protein engineering, and molecular evolution Life Sciences Biochemistry, general Cell Biology Science, general Life sciences Science Biochemistry Cell biology Genklonierung (DE-588)4123275-6 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Genklonierung (DE-588)4123275-6 s Methode (DE-588)4038971-6 s DE-604 Polymerase-Kettenreaktion (DE-588)4256726-9 s Chen, Bing-Yuan edt Janes, Harry W. edt Erscheint auch als Druck-Ausgabe 9780896039698 https://doi.org/10.1385/1592591779 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | PCR Cloning Protocols Life Sciences Biochemistry, general Cell Biology Science, general Life sciences Science Biochemistry Cell biology Genklonierung (DE-588)4123275-6 gnd Methode (DE-588)4038971-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4123275-6 (DE-588)4038971-6 (DE-588)4256726-9 (DE-588)4143413-4 |
| title | PCR Cloning Protocols |
| title_auth | PCR Cloning Protocols |
| title_exact_search | PCR Cloning Protocols |
| title_full | PCR Cloning Protocols edited by Bing-Yuan Chen, Harry W. Janes |
| title_fullStr | PCR Cloning Protocols edited by Bing-Yuan Chen, Harry W. Janes |
| title_full_unstemmed | PCR Cloning Protocols edited by Bing-Yuan Chen, Harry W. Janes |
| title_short | PCR Cloning Protocols |
| title_sort | pcr cloning protocols |
| topic | Life Sciences Biochemistry, general Cell Biology Science, general Life sciences Science Biochemistry Cell biology Genklonierung (DE-588)4123275-6 gnd Methode (DE-588)4038971-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Life Sciences Biochemistry, general Cell Biology Science, general Life sciences Science Biochemistry Cell biology Genklonierung Methode Polymerase-Kettenreaktion Aufsatzsammlung |
| url | https://doi.org/10.1385/1592591779 |
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