Muscle Gene Therapy: Methods and Protocols
Gene therapy offers many conceptual advantages to treat muscle diseases, especially various forms of muscular dystrophies; however, it faces a number of unique challenges, including the need to deliver a therapeutic vector to all muscles throughout the body. In Muscle Gene Therapy: Methods and Proto...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2011
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
709 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Gene therapy offers many conceptual advantages to treat muscle diseases, especially various forms of muscular dystrophies; however, it faces a number of unique challenges, including the need to deliver a therapeutic vector to all muscles throughout the body. In Muscle Gene Therapy: Methods and Protocols, expert researchers in the field present a collection of techniques aimed at bridging the translational gap in muscle gene therapy between the prevalent rodent models and vitally important larger animal models. Divided into three sections, this volume examines basic protocols for optimizing the muscle gene expression cassette and for evaluating the therapeutic outcomes, new developments in muscle gene therapy technology such as adeno-associated viral vector (AAV), oligonucleotide-mediated exon-skipping, and novel RNA-based strategies, and step-by-step guidance on muscle gene delivery in swine, ovine, canine, and non-human primates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, detailed, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Muscle Gene Therapy: Methods and Protocols serves as an invaluable resource for graduate students, post-doctoral fellows, and principle investigators pursuing the crucial advancement of muscle disease gene therapy in the hope of someday curing these debilitating disorders |
| Beschreibung: | 1 Online-Ressource (XIII, 382 p. 75 illus., 13 illus. in color) |
| ISBN: | 9781617379826 |
| DOI: | 10.1007/978-1-61737-982-6 |
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| 520 | |a Gene therapy offers many conceptual advantages to treat muscle diseases, especially various forms of muscular dystrophies; however, it faces a number of unique challenges, including the need to deliver a therapeutic vector to all muscles throughout the body. In Muscle Gene Therapy: Methods and Protocols, expert researchers in the field present a collection of techniques aimed at bridging the translational gap in muscle gene therapy between the prevalent rodent models and vitally important larger animal models. Divided into three sections, this volume examines basic protocols for optimizing the muscle gene expression cassette and for evaluating the therapeutic outcomes, new developments in muscle gene therapy technology such as adeno-associated viral vector (AAV), oligonucleotide-mediated exon-skipping, and novel RNA-based strategies, and step-by-step guidance on muscle gene delivery in swine, ovine, canine, and non-human primates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, detailed, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Muscle Gene Therapy: Methods and Protocols serves as an invaluable resource for graduate students, post-doctoral fellows, and principle investigators pursuing the crucial advancement of muscle disease gene therapy in the hope of someday curing these debilitating disorders | ||
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Datensatz im Suchindex
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|---|---|
| any_adam_object | |
| author2 | Duan, Dongsheng |
| author2_role | edt |
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| author_facet | Duan, Dongsheng |
| building | Verbundindex |
| bvnumber | BV044952387 |
| collection | ZDB-2-PRO |
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| dewey-full | 599.935 611.01816 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 599 - Mammalia 611 - Human anatomy, cytology, histology |
| dewey-raw | 599.935 611.01816 |
| dewey-search | 599.935 611.01816 |
| dewey-sort | 3599.935 |
| dewey-tens | 590 - Animals 610 - Medicine and health |
| discipline | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-61737-982-6 |
| format | Electronic eBook |
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| isbn | 9781617379826 |
| language | English |
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| physical | 1 Online-Ressource (XIII, 382 p. 75 illus., 13 illus. in color) |
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| spelling | Muscle Gene Therapy Methods and Protocols edited by Dongsheng Duan Totowa, NJ Humana Press 2011 1 Online-Ressource (XIII, 382 p. 75 illus., 13 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 709 Gene therapy offers many conceptual advantages to treat muscle diseases, especially various forms of muscular dystrophies; however, it faces a number of unique challenges, including the need to deliver a therapeutic vector to all muscles throughout the body. In Muscle Gene Therapy: Methods and Protocols, expert researchers in the field present a collection of techniques aimed at bridging the translational gap in muscle gene therapy between the prevalent rodent models and vitally important larger animal models. Divided into three sections, this volume examines basic protocols for optimizing the muscle gene expression cassette and for evaluating the therapeutic outcomes, new developments in muscle gene therapy technology such as adeno-associated viral vector (AAV), oligonucleotide-mediated exon-skipping, and novel RNA-based strategies, and step-by-step guidance on muscle gene delivery in swine, ovine, canine, and non-human primates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, detailed, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Muscle Gene Therapy: Methods and Protocols serves as an invaluable resource for graduate students, post-doctoral fellows, and principle investigators pursuing the crucial advancement of muscle disease gene therapy in the hope of someday curing these debilitating disorders Biomedicine Human Genetics Animal Genetics and Genomics Gene Therapy Medicine Human genetics Gene therapy Animal genetics Gentherapie (DE-588)4296957-8 gnd rswk-swf Muskeldystrophie (DE-588)4124922-7 gnd rswk-swf Muskelkrankheit (DE-588)4075131-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Muskelkrankheit (DE-588)4075131-4 s Muskeldystrophie (DE-588)4124922-7 s Gentherapie (DE-588)4296957-8 s Methode (DE-588)4038971-6 s 1\p DE-604 Duan, Dongsheng edt Erscheint auch als Druck-Ausgabe 9781617379819 https://doi.org/10.1007/978-1-61737-982-6 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Muscle Gene Therapy Methods and Protocols Biomedicine Human Genetics Animal Genetics and Genomics Gene Therapy Medicine Human genetics Gene therapy Animal genetics Gentherapie (DE-588)4296957-8 gnd Muskeldystrophie (DE-588)4124922-7 gnd Muskelkrankheit (DE-588)4075131-4 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4296957-8 (DE-588)4124922-7 (DE-588)4075131-4 (DE-588)4038971-6 |
| title | Muscle Gene Therapy Methods and Protocols |
| title_auth | Muscle Gene Therapy Methods and Protocols |
| title_exact_search | Muscle Gene Therapy Methods and Protocols |
| title_full | Muscle Gene Therapy Methods and Protocols edited by Dongsheng Duan |
| title_fullStr | Muscle Gene Therapy Methods and Protocols edited by Dongsheng Duan |
| title_full_unstemmed | Muscle Gene Therapy Methods and Protocols edited by Dongsheng Duan |
| title_short | Muscle Gene Therapy |
| title_sort | muscle gene therapy methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Animal Genetics and Genomics Gene Therapy Medicine Human genetics Gene therapy Animal genetics Gentherapie (DE-588)4296957-8 gnd Muskeldystrophie (DE-588)4124922-7 gnd Muskelkrankheit (DE-588)4075131-4 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Biomedicine Human Genetics Animal Genetics and Genomics Gene Therapy Medicine Human genetics Gene therapy Animal genetics Gentherapie Muskeldystrophie Muskelkrankheit Methode |
| url | https://doi.org/10.1007/978-1-61737-982-6 |
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